SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene10741

Length: 1718 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene10741

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	dead box ATP-dependent RNA helicase, putative	-	-	61%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	34%
Blast2go	dead box atp-dependent rna	-	-	80%

Source	GOs	Term	Type	Identity
Blast2go	auxin biosynthetic process	GO:0009851	Biological Process	80%
Blast2go	ATP-dependent helicase activity	GO:0008026	Molecular Function	80%
Blast2go	nucleic acid binding	GO:0003676	Molecular Function	80%
Blast2go	ATP binding	GO:0005524	Molecular Function	80%

Source	InterPros	Term	Type	Identity
Blast2go	RNA helicase, ATP-dependent, DEAD-box, conserved site	IPR000629	-	80%
Blast2go	Helicase, C-terminal	IPR001650	-	80%
Blast2go	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	80%
Blast2go	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	80%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKZ7

Fln msg: STOP codon was not found. Distance to subject end: 12 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 424 - 504

Fln protein: QNDKDFEGKSFEDLGLSEWAVQVCRELGMKKPTPVQQHCIPQIIKGKDVMGLAQTGSGKTAAFALPILHKLAENPYGVFALVITPTRELAFQLAEQFKAIGSPLRLRCTVVIGGMDMISQAQALMQRPHVVIATPGRIKSLLEDYPDVPKVFSRAKFLVLDEADRLLDVGFEEELKVVFQCLPTERQTLLFSATMTGDLRALHDLSGDKAYFYEAYEGFKTVESLNQQYVFVPENVKDVYLMHIVSTMEDNEIRSAIVFVSTCRTCHLLSLLLDELGLEAVALHSLKSQSARLAALQRFKSGQVPVLLATDVASRGLDIPTVDLVINYDIPRYPRDYVHRVGRTARAGRGGQAVSIVTQYQVKLVHDIEAVVGKQLEEYDCKEDDVLKGITQVYKAKRMATMRMVDDGFEAKAKERKEMKLKML

Protein Length: 425

Fln nts: TTTTTGAAGCGTCGTGACCTTGGCTGTCACTCATTTTCGTTCCATCTCTGCACATCACCTGAAAAGCGAACTTTTCAAGTGTCCGACCTTACAACATCGCAAACGTATTTTCGTCCCGCCTCTGTACATCGGCTGAAAAGCGCAAAGCGCACAGGATGCTACAACACCCAGCATAATCATGGACAAAATTTTCCATCAGTAGTCTAAGAGTAGAATATATATGTCTATGTATGCACATGGGGGAACTAGCATAATCATGGACAAGATTTTCCTTCAGTAGTCTAAGAGTAGAATATATCTGTCTATGTATGCACATGGAGGAACCAGAGGAAGAAACGGTACCAACGGCAGCAGTTCCATCCCTTTTCTCCAAAGGGGCACTGAAGAAAAACAAGAAGAATAAATTCTCCAATCCAGACCCTCCACAGACCAAGCTCAAACTAACAAAACGATAAGGACTTTGAGGGTAAGAGCTTTGAGGATTTGGGTTTATCAGAATGGGCTGTACAGGTATGCAGGGAGCTCGGGATGAAGAAGCCCACCCCGGTTCAACAGCACTGTATTCCTCAAATTATCAAAGGAAAAGACGTGATGGGTTTGGCCCAGACTGGCAGTGGTAAAACGGCCGCCTTTGCACTGCCCATACTGCATAAATTAGCGGAGAATCCATATGGCGTTTTTGCCCTGGTCATCACTCCTACCAGGGAGTTGGCTTTTCAGCTGGCCGAACAATTCAAAGCCATTGGATCACCTCTGCGTCTGCGGTGCACGGTCGTTATCGGTGGGATGGATATGATCAGTCAGGCCCAAGCCTTGATGCAGCGACCCCATGTTGTGATCGCCACCCCTGGGCGTATCAAGTCTCTCCTCGAGGACTATCCTGATGTCCCCAAAGTGTTTTCCAGGGCAAAGTTTCTTGTTTTGGATGAAGCAGATCGTTTACTGGATGTGGGTTTTGAAGAAGAACTGAAGGTAGTATTTCAGTGTTTGCCCACTGAACGTCAAACTCTTCTCTTTTCAGCGACAATGACTGGTGATCTAAGAGCCTTGCATGATTTATCTGGAGACAAAGCATACTTTTATGAGGCATACGAGGGATTTAAGACAGTTGAGTCATTGAACCAGCAATACGTTTTTGTTCCTGAAAATGTGAAGGATGTATATCTCATGCACATTGTTTCAACTATGGAAGATAATGAGATCAGATCTGCCATTGTTTTTGTTTCCACCTGCAGGACTTGCCATCTGCTTAGTTTATTGTTGGATGAACTTGGTTTGGAGGCAGTGGCATTGCATTCTCTCAAATCACAATCTGCAAGGCTTGCTGCTCTTCAGCGTTTCAAATCTGGGCAGGTTCCGGTTCTGTTAGCAACTGATGTAGCAAGTCGTGGTCTTGACATACCAACTGTTGATCTTGTCATCAATTATGATATACCAAGGTATCCTCGAGATTATGTTCATCGTGTAGGTCGTACAGCGAGAGCTGGAAGGGGAGGACAAGCTGTGAGCATAGTGACTCAGTATCAAGTGAAGCTTGTACATGATATAGAGGCTGTCGTTGGAAAACAGTTGGAGGAGTATGACTGTAAAGAGGATGATGTGCTGAAAGGTATCACACAGGTATATAAAGCCAAAAGGATGGCAACAATGAGGATGGTTGATGATGGTTTTGAGGCAAAAGCAAAAGAAAGGAAAGAAATGAAACTTAAAATGTTGGT

Fln Alignment:

sp_v1.1_unigene10741___KSFEDLGLSEWAVQVCRELGMKKPTPVQQHCIPQIIKGKDVMGLAQTGSGKTAAFALPILHKLAENPY-----GVFALVITPTRELAFQLAEQFKAIGSPLRLRCTVVIGGMDMISQAQALMQRPHVVIATPGRIKSLLEDYPDVPKVFSRAKFLVLDEADRLLDVGFEEELKVVFQCLPTERQTLLFSATMTGDLRALHDLSGDKAY-FYEAYEGFKTVESLNQQYVFVPENVKDVYLMHIVSTMEDNEIRSAIVFVSTCRTCHXXXXXXXXXXXXXXXXHSLKSQSARLAALQRFKSGQVPVLLATDVASRGLDIPTVDLVINYDIPRYPRDY
B8LKZ7_______________KNFGDAGFPDYVIEEIVKAGFTEPTPIQAQGWPMALKGRDLIGIAETGSGKTLAYLLPAIVHVNAQPYLAPGDGPIVLVLAPTRELAVQIQQEAAKFGASSKIKNTCIYGGAPKGPQVRDLSKGVEIVIATPGRLIDMLESQ---HTNLRRVTYLVLDEADRMLDMGFEPQIKKIVSQIRPDRQTLYWSATWPKEVELLARQSLHNPYKVIIGSSDLKANHAIEQIVEIVSEHEKYTRLIQLLEEIMDGS--RLLIFLETKKGCDQVTRKLRMDGWPALSIHGDKSQAERDWVLSEFKAGKSPIMTATDVAARGLDVKDIKCVINYDFPGSMEDY

SNPs (tot: 3)

Position	A %	C %	G %	T %	Probability
516		20		80	0.995493
624	66		33		0.997452
1400	62			37	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain