SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene10730

Length: 2373 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene10730
CGTCGTGACCTTGGCTGTCACTCAAGTTAACAGTATATTAAAGGCTGGTGGCTCTCGGCATTTGGTTCCTTTGAAAGGGCCATTTCAATCACCTTCAAAAAGTTCTGCATGGCAATCAAGCGGCATTACATCCTCACGGGGTAAGAATTTTGGAATGGATCAGGGTCTTGGTACAAAATCTGGTAAACTAGTTCATTCTGCTGATGCCTTTCCATTGCCAACCAATGTTGTTATTTCAACTAAGTCTACACAGAACATGGGTTCTGCTTGGAATAGAACGGATTGGACAAAACCTTGGGGTAAAGAAAGCAATACCGTTGCAGAGGATCCTTCCAGACAAGAAAATAATGGTACCAGTGGTAAGGGGGAGAAGGGTTCTGGCAACTTCGAACCACAGAAACTTGCAGGAAATGTTACCGTCAAGAGTCCTGGAATCAACCAGTCACAACCTCCTGTGGGAATTCAATTGCAACCATTATCGCCGGTGTCAAATCAGACCAATTCGTGGGAACATCATACTACAAGCTCTTTGACATCATGCTTGACAGCTTCTTCTGCAGATGCTGGAAGTCCACTGGAGCAAAAGAGTCATACAGATGATGGAAAGAAATGTGTCTCACCAGTGTCATCATCTCCAAGTCATATTTGTTTTGGATCAGCTGTACCTGTCTTACTTCCTCCTGTAGATACGATGCCAATTATCACTAACTCAGTTCCAGATTGTATGTCCCAAGCTATGAAAAGTTCATACAATAACATGTCTCCCCCTGCTACTATATCACCACATAACTTGTTGCCAATGGTTGTTAGCTCTTCTGAGGAACTTTTAGCTCCACTAAATCCCGTCTCCTTGCACATTCCTGTTTCTGAATCAATGAGCAACTTTTCATTGGGCCCTGAAACTAAACTTGTCTCTGGCACACAAACACCTCGTGTCTCGTCCCCTATTTGTGGACCTGTTTCTGCTCCAATACAGAGACCTGCACCCATTGAGTCTCCCATCTCTAAACTACTGCCTTCAGAACTTGTTGCTGAAGTGCATGTTGCTGGAGATCAAATCCATCCAATCACGGAGCCTTGGGGTTTGCGGTCATTGTCTCTAGATGGTGGAGATGGTGTATTCAAATGGCAAATGTGGGACACACCGCAGCTTGGACAGGGCAACCTTCCCAATGAAGCAAGCCTCCTTCTTGGTTACCTGTTGGTAATGGCATGCTAGAACAGGAAAAAGATTTTCTGCTCCCACTTCCCGAAAGAAATTTACTTTCTCCATTTGAACCAGAAAGTCGTCTTCCCTCTTGTCTGTTTACTCCGCTACAGAACCCCAACGACATCACTCCAAGTAGTGAAGACTACATAGACGTAGCATCAGGTTCAAGTAGTAATAGCTTGTGGTTGAATCAGCCAGCTTTTGATTCTGCTGAAGGAAATTGGAGTCATGTTGACCAGCATCGGGTATCACCCAGTTTTTCGAATGGAGTTACCCAGGGAGAAGTGCAAGATTCTGTGGTCACCACTGATCATCATTCATATGAGCAAATTCCAACAAGTGCCTGGCTCAAGTCTGGGATAGATGGCATGGAGAAAGGTAAATGGTTGGCAGAAACAGCAGTGGGAAGTTCATCCTGGTCAAGTACAATAAAGGCACCAACAAGGCCACATATTGGAGGCATTTATACCACTCCTGATGTACAGTCCGTTTGGTCATACAAGTAACTTTGTTGTTGCATGACTTGAACTTCTCCATTTTGATCCTCTGATTATTCCATGTTGGAACTTTATTGAGCACTGTTGTCCTTTGAATTATCAAGATAACCGTGCTGGGTAATCTTGCTTCTGTTGTCAGTAATGTAAGGTCACTCTGTATGGGGGATAAGATGATTTGAATACATACAGAGATTTGCATGGAGATCATTTGTATGAGGCTGGAAATTATGGCCTGCTGATGGCTATCATGAAAGGAATGAAGAGTACAAAGGGTTCAGTTTTCTGAATCAGATGTTCAGGATGCATTTGATGTCATTTTCATTTTTCTCATGGATCATGCTCTCTGCTTAAATATATACCATGCAGAAAGAGTTTCTAGAATGTTTCCAAGACCTTATTCTACTTTGCAACACAGCGAACCCATCGTGTTCATCATTTACATTTGATTTTCCAATCGAGGAGATGATTTTATCGTCCATGATTCTGTATCTTGCAAGCACAATTGTCATCATTTTTCATTTTGTATTTCCTTCACCTGTGAAGAACATGCTGCTTGTGTTCTTTGCCATATTCAGAATCTTGGAGCTGAGTATTGGTTGCAAAAATACCTAACGAAAAGGTACCCCCAAGACCTAAGCAAAATATAACCCCTTAGTAGGTAATATG

Ace file of the UniGene sp_v1.1_unigene10730 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Unassigned protein	-	-	0.0	-
FL-Next	tr=Putative uncharacterized protein; Ricinus communis (Castor bean).	-	-	0.0	28%
Blast2go	conserved hypothetical protein [Ricinus communis]	-	-	4.44857e-18	43%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: tr_plants

Fln subject: B9T651

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 150 aas, putative chimeric sequence, your sequence is shorter than subject: 406 - 883

Fln protein: VVTLAVTQVNSILKAGGSRHLVPLKGPFQSPSKSSAWQSSGITSSRGKNFGMDQGLGTKSGKLVHSADAFPLPTNVVISTKSTQNMGSAWNRTDWTKPWGKESNTVAEDPSRQENNGTSGKGEKGSGNFEPQKLAGNVTVKSPGINQSQPPVGIQLQPLSPVSNQTNSWEHHTTSSLTSCLTASSADAGSPLEQKSHTDDGKKCVSPVSSSPSHICFGSAVPVLLPPVDTMPIITNSVPDCMSQAMKSSYNNMSPPATISPHNLLPMVVSSSEELLAPLNPVSLHIPVSESMSNFSLGPETKLVSGTQTPRVSSPICGPVSAPIQRPAPIESPISKLLPSELVAEVHVAGDQIHPITEPWGLRSLSLDGGDGVFKWQMWDTPQLGQGNLPNEASLLLGYLLVMAC*

Protein Length: 407

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAGTTAACAGTATATTAAAGGCTGGTGGCTCTCGGCATTTGGTTCCTTTGAAAGGGCCATTTCAATCACCTTCAAAAAGTTCTGCATGGCAATCAAGCGGCATTACATCCTCACGGGGTAAGAATTTTGGAATGGATCAGGGTCTTGGTACAAAATCTGGTAAACTAGTTCATTCTGCTGATGCCTTTCCATTGCCAACCAATGTTGTTATTTCAACTAAGTCTACACAGAACATGGGTTCTGCTTGGAATAGAACGGATTGGACAAAACCTTGGGGTAAAGAAAGCAATACCGTTGCAGAGGATCCTTCCAGACAAGAAAATAATGGTACCAGTGGTAAGGGGGAGAAGGGTTCTGGCAACTTCGAACCACAGAAACTTGCAGGAAATGTTACCGTCAAGAGTCCTGGAATCAACCAGTCACAACCTCCTGTGGGAATTCAATTGCAACCATTATCGCCGGTGTCAAATCAGACCAATTCGTGGGAACATCATACTACAAGCTCTTTGACATCATGCTTGACAGCTTCTTCTGCAGATGCTGGAAGTCCACTGGAGCAAAAGAGTCATACAGATGATGGAAAGAAATGTGTCTCACCAGTGTCATCATCTCCAAGTCATATTTGTTTTGGATCAGCTGTACCTGTCTTACTTCCTCCTGTAGATACGATGCCAATTATCACTAACTCAGTTCCAGATTGTATGTCCCAAGCTATGAAAAGTTCATACAATAACATGTCTCCCCCTGCTACTATATCACCACATAACTTGTTGCCAATGGTTGTTAGCTCTTCTGAGGAACTTTTAGCTCCACTAAATCCCGTCTCCTTGCACATTCCTGTTTCTGAATCAATGAGCAACTTTTCATTGGGCCCTGAAACTAAACTTGTCTCTGGCACACAAACACCTCGTGTCTCGTCCCCTATTTGTGGACCTGTTTCTGCTCCAATACAGAGACCTGCACCCATTGAGTCTCCCATCTCTAAACTACTGCCTTCAGAACTTGTTGCTGAAGTGCATGTTGCTGGAGATCAAATCCATCCAATCACGGAGCCTTGGGGTTTGCGGTCATTGTCTCTAGATGGTGGAGATGGTGTATTCAAATGGCAAATGTGGGACACACCGCAGCTTGGACAGGGCAACCTTCCCAATGAAGCAAGCCTCCTTCTTGGTTACCTGTTGGTAATGGCATGCTAG___AACAGGAAAAAGATTTTCTGCTCCCACTTCCCGAAAGAAATTTACTTTCTCCATTTGAACCAGAAAGTCGTCTTCCCTCTTGTCTGTTTACTCCGCTACAGAACCCCAACGACATCACTCCAAGTAGTGAAGACTACATAGACGTAGCATCAGGTTCAAGTAGTAATAGCTTGTGGTTGAATCAGCCAGCTTTTGATTCTGCTGAAGGAAATTGGAGTCATGTTGACCAGCATCGGGTATCACCCAGTTTTTCGAATGGAGTTACCCAGGGAGAAGTGCAAGATTCTGTGGTCACCACTGATCATCATTCATATGAGCAAATTCCAACAAGTGCCTGGCTCAAGTCTGGGATAGATGGCATGGAGAAAGGTAAATGGTTGGCAGAAACAGCAGTGGGAAGTTCATCCTGGTCAAGTACAATAAAGGCACCAACAAGGCCACATATTGGAGGCATTTATACCACTCCTGATGTACAGTCCGTTTGGTCATACAAGTAACTTTGTTGTTGCATGACTTGAACTTCTCCATTTTGATCCTCTGATTATTCCATGTTGGAACTTTATTGAGCACTGTTGTCCTTTGAATTATCAAGATAACCGTGCTGGGTAATCTTGCTTCTGTTGTCAGTAATGTAAGGTCACTCTGTATGGGGGATAAGATGATTTGAATACATACAGAGATTTGCATGGAGATCATTTGTATGAGGCTGGAAATTATGGCCTGCTGATGGCTATCATGAAAGGAATGAAGAGTACAAAGGGTTCAGTTTTCTGAATCAGATGTTCAGGATGCATTTGATGTCATTTTCATTTTTCTCATGGATCATGCTCTCTGCTTAAATATATACCATGCAGAAAGAGTTTCTAGAATGTTTCCAAGACCTTATTCTACTTTGCAACACAGCGAACCCATCGTGTTCATCATTTACATTTGATTTTCCAATCGAGGAGATGATTTTATCGTCCATGATTCTGTATCTTGCAAGCACAATTGTCATCATTTTTCATTTTGTATTTCCTTCACCTGTGAAGAACATGCTGCTTGTGTTCTTTGCCATATTCAGAATCTTGGAGCTGAGTATTGGTTGCAAAAATACCTAACGAAAAGGTACCCCCAAGACCTAAGCAAAATATAACCCCTTAGTAGGTAATATG

Fln Alignment:

sp_v1.1_unigene10730___ICFGSAVPVLLPPVDTMPIITNSVPDCMSQAMKSSYNNMSPPATISPHNLLPMVVSSSEELLAPLNPVSLHIPVSESMSNFSLGPETKLVS--GTQTPRVSSPICGPVSAPIQRPAPIESPISKLLPSELVAEVHVAGDQIHPITEPWXXXXXXXXXXXGVF-----KWQMWDTPQLGQGNL
B9T651_______________ISFGLPSPFTIP---TYPSVSSS----SSLGFSPPIEGIFPRGVDGPHEIIPEEPELFEDPCYVPDPISLLGPVSESLADFQFDLGTGFTSDIGLERPHALKNL--STSPEVSKPSPIESPLSRL----RVADEKHNGSNWFPTTPKAQDSHNLPMDDVHVHANEKGTWQMWNSP-LGQDGL

SNPs (tot: 65)

Position	A %	C %	G %	T %	Probability
291			16	83	0.994512
292	16	83			0.994512
293			16	83	0.994512
294	85			14	0.993533
297	85	14			0.993533
299		90	10		0.90298
300		10		90	0.90298
302	10			90	0.90298
303	10			90	0.90298
304			10	90	0.90298
305		90	10		0.90298
310		10		90	0.90298
312	10		90		0.90298
313		10		90	0.90298
314	90		10		0.90298
383	85			14	0.993533
387	85		14		0.993533
424			75	25	1.0
502		10		90	0.90298
503		10		90	0.90298
524	10			90	0.90298
609	85		14		0.999981
757	90			10	0.90298
768		10	90		0.90298
770		11		88	0.923564
771	11		88		0.923564
887		14		85	0.993533
890	14	85			0.993533
915	14		85		0.993533
920		14		85	0.993533
922		85	14		0.993533
925	14		85		0.993533
927			14	85	0.993533
930	16			83	0.994512
931		16	83		0.994512
932	16		83		0.994512
933	16			83	0.994512
934		83		16	0.994512
935	66		33		1.0
938	80		20		0.995493
943	14			71	0.984337
944		71	14	14	0.984337
946	71	28			1.0
948	14			85	0.993533
949			28	71	1.0
950	14			71	0.984337
951		71	28		1.0
952		71		28	1.0
953		14	14	71	0.984337
954	28			71	1.0
959	81	18			0.999994
962	90		10		0.90298
967	90		10		0.90298
969	90	10			0.90298
974	11		88		0.923564
975		88	11		0.923564
983	88		11		0.923564
989	88		11		0.923564
991		88		11	0.923564
992		11		88	0.923564
994		11		88	0.923564
995			11	88	0.923564
998			11	88	0.923564
1065		75		25	1.0
1105	83	16			0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain