SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene10668

Length: 1165 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene10668

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Short-chain type dehydrogenase/reductase n=1 Tax=Picea abies RepID=SDR1_PICAB	-	-	55%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	57%
Blast2go	short-chain type	-	-	70%

Source	GOs	Term	Type	Identity
Blast2go	metabolic process	GO:0008152	Biological Process	70%
Blast2go	response to arsenic-containing substance	GO:0046685	Biological Process	70%
Blast2go	oxidoreductase activity	GO:0016491	Molecular Function	70%

Source	InterPros	Term	Type	Identity
Blast2go	Short-chain dehydrogenase/reductase SDR	IPR002198	-	70%
Blast2go	Glucose/ribitol dehydrogenase	IPR002347	-	70%
Blast2go	NAD(P)-binding domain	IPR016040	-	70%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABB4

Fln msg: Unexpected STOP codon in 5 prime region,

Fln protein: QRRREGERTTVEMKKPLEGRVAVVTGASRGLGAEIALHLAADGANVVVNYLNNADKAEQIASAINSSRGHMAAIAVRADVSSSGDVTRLFDKAEEIFGVVHIVVNNAGIIDSTCATIAETSPEAWDATFSANCRAPFLTCKEAARRLLPHGGGRIINISGTLVATPRPGFGAYTASKAAMETMTHILARELEGTGVTANCVAPGPIATDMFFAVRDEESVKRIAMSPPLERLGDCKDIARVVAFLARPESESINGQILRVNGGLA*

Protein Length: 267

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTTAGGGTACCAATAGGTTGTTGACAACGACGGAGGGAAGGGGAGAGGACTACTGTGGAGATGAAGAAGCCACTCGAGGGTCGAGTGGCGGTCGTGACCGGCGCCTCTCGCGGCCTGGGCGCCGAGATTGCCCTTCACCTGGCTGCCGACGGCGCCAATGTTGTGGTCAACTACCTGAACAACGCAGACAAAGCAGAACAGATCGCCTCTGCTATCAATTCATCCCGTGGTCACATGGCGGCCATTGCCGTGAGAGCCGATGTTTCAAGCTCTGGGGATGTGACCCGGCTATTCGACAAGGCGGAGGAAATTTTCGGAGTAGTTCACATAGTAGTCAATAATGCAGGGATCATTGACTCCACCTGCGCAACGATAGCGGAGACCAGCCCGGAGGCGTGGGACGCCACTTTCAGCGCCAATTGCAGAGCCCCTTTTCTCACCTGCAAAGAGGCCGCCAGGAGGCTCCTCCCTCACGGCGGCGGGCGGATCATCAACATCTCGGGCACTCTGGTGGCCACGCCCAGGCCAGGCTTCGGGGCTTACACGGCGAGCAAAGCAGCAATGGAGACCATGACTCATATCCTGGCGAGGGAGCTTGAGGGTACGGGAGTCACGGCTAACTGCGTAGCCCCTGGTCCTATTGCTACCGACATGTTCTTCGCCGTTAGGGATGAGGAGTCCGTTAAGCGCATTGCCATGTCCCCTCCTCTGGAACGGCTCGGCGATTGCAAGGACATTGCTCGCGTGGTTGCTTTCTTGGCTCGCCCTGAGAGTGAGTCCATCAATGGCCAAATCCTTCGCGTTAATGGAGGTCTGGCCTGA___GTTTCATTTCCATTCTTGCTATTAAAACACGAAATTTCTCAGCTTTGTTTTGTGCCTGAGTTTCATTTCCTTTCTTGCTATTAAAACACGAAATTTCTCAGCTTTGTTTTGTTTTTCTATATGCTTGTTCTGTTAATTGCTTAAAGCCCTAAGGGTACATGTAAAATGGTTACCCTAATGGTAAATCCCTGGCCTGTATTTGCAAGCATTAACTTTGAAAAATTTAGTGTTTTTCTTTGTATTACGAAGTTGTATGGATCCTGATAGGTACCATGACCATATGTCAAACTTAATCTACTCCACTTCGTTACACATT

Fln Alignment:

sp_v1.1_unigene10668___QRRREGERTTVEMKKPLEGRVAVVTGASRGLGAEIALHLAADGANVVVNYLNNADKAEQIASAIN---SSRGHMAAIAVRADVSSSGDVTRLFDKAEEIFGVVHIVVNNAGIIDSTCATIAETSPEAWDATFSANCRAPFLTCKEAARRLLPHGGGRIINISGTLVATPRPGFGAYTASKAAMETMTHILARELEGTGVTANCVAPGPIATDMFFAVRDEESVKRIAMSPPLERLGDCKDIARVVAFLARPESESINGQILRVNGG
D5ABB4_______________EQKKRSEEEKRGMTMPLEGRVAIVTGASRGIGREIALHLAEKGAKVVVNYSSNQGPAEEVASAINNLASSGDGVRAIVCKADVAEPSQVAQLFDTAEHAFGPLHIVVNNAGVTDSKYPTLAQTSDEDWDRIFQVNCKGAFLCSREAAKRVVRGGGGRIINMSSSLVGMPIPGFGAYTASKAAMEMMTRILAQELRGTKITANCVAPGAVATDLFLAGRSKAAVEAVAESSPFERLGKVEDVAPVVAFLASDQGEWVNGQVVRVNGG

SNPs (tot: 22)

Position	A %	C %	G %	T %	Probability
189			25	75	0.996473
191			25	75	0.996473
222	50		50		1.0
294	50	50			1.0
310	50		50		1.0
342		50		50	1.0
408			66	33	0.997452
409	66			33	0.997452
415		66		33	0.997452
416		66	33		0.997452
417	33	66			0.997452
427		33	66		0.997452
512	25	50			0.995533
513			50	25	0.995533
516		75	25		0.996473
618	14		85		0.993533
619		85		14	0.993533
621	85		14		0.993533
763	88			11	0.923564
910		87		12	0.992554
994		83		16	0.994512
1045			80	20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain