SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene10331

Length: 1896 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene10331

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	ALATS; ALATS (ALANYL-TRNA SYNTHETASE); ATP binding / alanine-tRNA ligase/ ligase, forming aminoacyl-tRNA and related compounds / nucleic acid binding / nucleotide binding (EC:6.1.1.7); K01872 alanyl-tRNA synthetase [EC:6.1.1.7]	-	-	60%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	24%
Blast2go	cytosolic trna-ala synthetase	-	-	77%

Source	ECs	Term	Type	e value	Identity
Blast2go	Alanine--tRNA ligase.	EC:6.1.1.7	-	0.0	77%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Aminoacyl-tRNA biosynthesis	00970		0.0	77%

Source	ECs	Term	Type	e value	Identity
AutoFact	Alanine--tRNA ligase.	EC:6.1.1.7	-	0.0	60%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Aminoacyl-tRNA biosynthesis	00970		0.0	60%

Source	GOs	Term	Type	Identity
Blast2go	alanyl-tRNA aminoacylation	GO:0006419	Biological Process	77%
Blast2go	alanine-tRNA ligase activity	GO:0004813	Molecular Function	77%
Blast2go	nucleic acid binding	GO:0003676	Molecular Function	77%
Blast2go	ATP binding	GO:0005524	Molecular Function	77%
Blast2go	mitochondrion	GO:0005739	Cellular Component	77%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNN6

Fln msg: W2: There is no M at the beginning, your sequence is longer than subject: 473 - 258

Fln protein: VPTDDRPKYLWNQVHNSVVKAIYDGSKFVHSVSEGEEVGIVLESTSFYAEQGGQIYDTGSCEGPSGTFDVRNVQIFGGYVLHMGSLVGETGSISVGDEVICKVDYARRTSIAPNHTCTHMLNYALKAVLGDHVDQKGSLVVPDKLRFDFSHGKPIPASDLRKIESIVNKQIKDELEVYAKEASLMEAKRIMGLRAVFGEVYPDPVRVVSIGRPVEDLLVDPENPEWGSFSTEFCGGTHLSNTREAKAFALVSEEGIAKGVRRITAFTTVAALQAIEAAASFDAYINNASTLDGIALEKEVASMKSALDSAIIPAAKKAEFRVKISELQDRVKKAQKQAAEANLQAAISTAIEFIDKLTLQNEASCVLRIDVGLDTNAVREALLKIMEKQKDIPVMVFSVDEATNKVLVYAGVPESVQKKGLVVLDWLRKVLEPLKGKGGGGKGGLAQGQGNDATRIEEAMKIARTMAGEKLT*

Protein Length: 474

Fln nts: TGGTTCCGACAGATGACAGGCCCAAGTATTTGTGGAATCAGGTCCATAATAGTGTGGTCAAGGCGATCTATGATGGGTCAAAGTTTGTACACTCTGTGTCAGAAGGCGAGGAAGTTGGTATTGTGCTTGAAAGTACTAGTTTCTATGCAGAGCAGGGAGGACAGATTTATGATACTGGAAGCTGTGAAGGTCCATCTGGAACTTTTGATGTCAGGAATGTCCAAATCTTTGGTGGATATGTGCTTCATATGGGCTCTCTGGTTGGTGAAACTGGCAGTATTTCAGTTGGGGATGAAGTCATTTGCAAGGTAGATTATGCAAGGCGGACTAGTATCGCTCCAAACCATACTTGCACTCATATGCTAAACTATGCACTGAAGGCTGTACTTGGAGATCACGTCGATCAAAAAGGATCCTTGGTGGTTCCAGACAAGTTGCGCTTTGATTTCTCTCATGGTAAACCTATTCCTGCCAGTGATCTGCGAAAAATAGAATCAATTGTGAATAAGCAGATTAAAGATGAGCTAGAAGTATATGCAAAAGAAGCTAGCCTAATGGAGGCAAAACGCATCATGGGGCTTCGTGCTGTGTTTGGTGAAGTCTATCCTGATCCTGTTCGAGTAGTTTCTATTGGAAGACCTGTTGAGGATCTTCTTGTAGATCCGGAGAACCCAGAATGGGGATCATTTTCAACAGAATTTTGTGGTGGAACACACCTATCAAATACAAGAGAGGCAAAAGCATTTGCTCTTGTATCAGAAGAGGGGATTGCAAAGGGTGTGCGGAGGATTACAGCCTTCACAACTGTAGCAGCTCTTCAGGCAATTGAAGCAGCAGCATCCTTTGATGCATATATAAACAATGCCTCAACTCTTGATGGAATTGCATTAGAGAAGGAAGTAGCTTCTATGAAGAGTGCACTGGATTCTGCCATCATTCCAGCAGCTAAAAAGGCAGAGTTCAGGGTGAAAATTTCTGAACTGCAGGATCGAGTTAAAAAGGCTCAAAAGCAAGCTGCAGAAGCTAATCTGCAGGCAGCAATAAGTACAGCGATTGAATTCATTGACAAATTAACTTTGCAGAATGAAGCTTCATGTGTATTACGAATTGATGTGGGTTTGGATACCAATGCTGTCAGAGAAGCACTCCTAAAAATTATGGAGAAGCAGAAGGACATTCCAGTCATGGTGTTTAGTGTAGATGAGGCAACAAATAAAGTCCTTGTCTATGCTGGTGTTCCAGAATCAGTACAAAAGAAAGGTTTAGTAGTATTGGATTGGCTGAGGAAAGTGTTGGAGCCACTGAAGGGAAAGGGTGGCGGTGGCAAAGGTGGATTGGCACAGGGACAGGGGAATGATGCTACTAGAATAGAAGAGGCTATGAAAATTGCTCGGACTATGGCAGGAGAGAAACTGACTTGA___TAACTCAGGTTGACAGTTACATTACTATAGTGAGTAATTAGCAGGTACAAAATGAATATTTTTAATTTCTCAAAGAGATGTAGAAGGATGGATATTATGTGAGGTATGATTATTTTCTCAGAGGTGGAGTGATGGATATTAATAGGTGTGGTAACTCCATTTATTGGCATTTTGCCTCATCGGTATTTATTCCTCTGAGAGTGGTTCTGTGAAAGAAGATTTGCTGCAACTGCTAATTTCAATTTATTTTTCTGCCCTGTATAGATCAACTTCACTCATGAATGGGGTGCATGTAAATGGATATCTTCTTCCTTAAAGGAAGAGTAATTCTTAAAACAATTCTCAAATGTACATCCCAAGTGGATTCACAATGCCCTCCACTGGCTTGTGGGGTAATCTCCAAATTATTTCTTATAGCTTTGAATTGTATTTTATAACAAAATATTTTTTGAAAAAATGTATTTTAAGTTTATAT

Fln Alignment:

sp_v1.1_unigene10331___SKFVHSVSEGEEVGIVLESTSFYAEQGGQIYDTGSCEGPSG--TFDVRNVQIFGGYVLHMG---SLVGETGSISVGDEVICKVDYARRTSIAPNHTCTHMLNYALKAVLGDHVDQKGSLVVPDKLRFDFSHGKPIPASDLRKIESIVNKQIKDELEVYAKEASLMEAKRIMGLRAVFGEVYPDPV------RVVSIGRPVEDLLVDPENPEWGSFSTEFCGGTHLSNTREAKAFALVSEEGIAKGVRRIT
B8LNN6_______________ASFISMVADGERLAVIVDSTILYPQGGGQPADIGFIYDLDERLKFRVQDVRSRNGVVFHYGYFDSPLSEQSTFQKGQEVILSVDEHRRGLNSRLHSAGHLLDVCMHNIGLASLESGKGHHFPDGAFVEYK-GK-IPADDLERKREALEKEAKRLVSIGGKVSAAIVPYE--SAAALCGGELPEYIPKDTTPRIVMLG----DNLGCP------------CGGTHVADISDI-GIIKVSQIRVRKGLTKVS

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
573	10		90		0.90298
575	90	10			0.90298
909		90		10	0.90298
1004	85			14	0.993533
1005	85		14		0.993533
1081			12	87	0.999964
1083		12		87	0.999964
1470		20	80		0.995493
1473	80		20		0.995493
1474		80		20	0.995493
1475	80		20		0.995493
1477	80	20			0.995493
1478	80	20			0.995493
1481	80		20		0.995493
1482	80	20			0.995493
1484	80		20		0.995493
1486		20		80	0.995493
1488		20		80	0.995493
1489	20			80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain