SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene10069

Length: 1716 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene10069 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	D8-sphingolipid desaturase n=1 Tax=Jatropha curcas RepID=A4UIY7_9ROSI	-	-	58%
FL-Next	tr=D8-sphingolipid desaturase; Jatropha curcas.	-	-	68%
Blast2go	fatty acid	-	-	71%

Source	GOs	Term	Type	Identity
Blast2go	lipid metabolic process	GO:0006629	Biological Process	71%
Blast2go	oxidoreductase activity	GO:0016491	Molecular Function	71%
Blast2go	iron ion binding	GO:0005506	Molecular Function	71%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: tr_plants

Fln subject: A4UIY7

Fln msg: Separated hits, possible frame ERROR between 416 and 436,

Fln protein: MEELRSRKISLEELKAHDKEGDLWVSIHGKVYDVTEWLHKHPGGDLPVLNLGGQDVTDAFIAYHPGSAWKLLDQFLIxxxxxxxxVSEISKDYRKILGDLKTAGMFQHSLSDYVLTVLFMVAMMGACIVGVVFYEGFWVHVGCGIILGLVWIQSAFIGHDSGHSELVGHKKIDKFIQLFAGNGLTGIGMGWWKRSHNVHHISCNSLDFDPDLQYVPLFAVSSGYFGSLHSYYYDKQMNFDGLARFLVSYQHWTFYPTMAFARVLLFMQSIVLHLSKNRVPNKGLDIAGLLFFWTWFSLLVSCLPSWPERIAFVLSSFAVTGIQHVQFCLNHFSAESYVGRPCSTSWIGDQARGTIDIQCGPWMDWFHGGLQFQVEHHMFPRLPRYNLRKISPLVKSFCAKHQLPYTMVTFWEANVRTVATLRAAALEARDASQTTPKNLLWEALNTHG*

Protein Length: 450

Fln nts: GAGTGATCATAAATTCCATGTCAAAGGAGTAGTAGACATCTGGGATTCATGTGAAGAGTGGTCATAAATTCCAGTTTAGCCCGTGATCTAAACAGATAGCTTTATATATCAGAGACCTAATTGAAAAAGCCAGGAGCCCCTGCTATTGGATCTGAGATTAGTCCGCCATAGCAGTGACCGCC_-_ATGGAAGAGCTTAGGAGCAGGAAAATCAGCTTAGAAGAGCTCAAAGCCCACGACAAAGAAGGAGACTTATGGGTTTCAATACACGGGAAAGTGTACGACGTTACAGAATGGCTACACAAGCACCCAGGGGGGGATCTGCCTGTGTTAAACCTAGGAGGCCAAGATGTCACAGATGCTTTCATTGCCTATCATCCAGGCTCTGCATGGAAGCTTTTGGATCAGTTCTTGATTGGxxxxxxxxxxxxxxxxxxxxxGGTCTCTGAGATCTCCAAGGACTACAGGAAGATTCTGGGGGATCTGAAGACGGCTGGGATGTTTCAGCATTCTTTGTCTGATTATGTCTTGACAGTGCTATTTATGGTGGCAATGATGGGGGCCTGTATTGTGGGTGTGGTGTTTTATGAAGGGTTTTGGGTACATGTGGGATGTGGGATTATATTGGGGTTGGTATGGATTCAGAGTGCTTTTATAGGCCATGATTCAGGCCACTCTGAGCTTGTGGGTCATAAGAAAATTGATAAGTTTATTCAGTTATTTGCAGGTAATGGCTTGACCGGGATTGGTATGGGCTGGTGGAAGAGAAGTCATAATGTCCATCATATTTCTTGCAATAGTTTAGACTTTGATCCTGATCTTCAATATGTACCCTTGTTTGCAGTCTCCTCTGGTTATTTTGGTTCTTTGCATTCTTATTATTATGACAAGCAAATGAATTTTGATGGCCTTGCTAGGTTTCTTGTAAGTTACCAGCATTGGACATTTTATCCTACCATGGCCTTTGCCAGGGTACTTCTGTTTATGCAGTCCATTGTATTACATTTGTCCAAAAATAGGGTACCCAACAAGGGTTTAGATATTGCAGGGCTTTTGTTCTTCTGGACTTGGTTTTCGTTGCTCGTGTCCTGCCTGCCTTCCTGGCCGGAAAGAATTGCCTTCGTGCTCTCCAGCTTCGCGGTGACCGGAATCCAGCATGTCCAGTTCTGTTTGAATCATTTTTCGGCGGAGAGCTATGTCGGAAGGCCGTGCAGCACATCGTGGATTGGAGATCAGGCGAGGGGAACGATCGATATACAGTGTGGCCCGTGGATGGATTGGTTTCACGGGGGATTGCAGTTTCAGGTGGAGCACCATATGTTCCCGCGGTTGCCTCGCTACAATCTCAGAAAGATTTCTCCCCTTGTGAAATCGTTCTGTGCCAAGCACCAATTGCCGTACACCATGGTCACCTTCTGGGAGGCCAATGTGAGAACCGTTGCCACTCTGCGCGCTGCAGCACTCGAAGCCCGAGACGCCTCTCAGACGACACCCAAAAATCTGTTGTGGGAGGCTCTTAATACCCATGGCTAG___CATTGGGTGCGCCTGTCCAGAGTGTCTATGGCCAGCATAGTGCGATGATTAGGTGGGCCTGTTAAGTCACTCTCAGACATTAAGTTATTTGTTTAAATCTCATTTTATAGGGTTGTTATTCTTGAATGTAATAATTCTCTACTTTATACTGTTGTTATTTTTTAATATAATCATGATATGACTTTACCA

Fln Alignment:

sp_v1.1_unigene10069___ISLEELKAHDKEGDLWVSIHGKVYDVTEWLHKHPGGDLPVLNLGGQDVTDAFIAYHPGSAWKLLDQFLIGxxxxxxxVSEISKDYRKILGDLKTAGMFQHSLSDYVLTVLFMVAMMGACIVGVVFYEGFWVHVGCGIILGLVWIQSAFIGHDSGHSELVGHKKIDKFIQLFAGNGLTGIGMGWWKRSHNVHHISCNSLDFDPDLQYVPLFAVSSGYFGSLHSYYYDKQMNFDGLARFLVSYQHWTFYPTMAFARVLLFMQSIVLHLSKNRVPNKGLDIAGLLFFWTWFSLLVSCLPSWPERIAFVLSSFAVTGIQHVQFCLNHFSAESYVGRPCSTSWIGDQARGTIDIQCGPWMDWFHGGLQFQVEHHMFPRLPRYNLRKISPLVKSFCAKHQLPYTMVTFWEANVRTVATLRAAALEARDASQTTPKNLLWEALNTHG
A4UIY7_______________ISEEELKKHTKSGDLWISIQGKVYNVTGWTKDHPGGELPLLHLSGQDVTDAFVAYHPGTAWQYLDKFFTGxxxxxxxVSEVSKDYRKLLAEFTKMGLFEKKGHVTLVTLCVVAMLLSFSIYGILCSDSTWVHLLSGGLIGFLWIQSGWIGHDSGHYQVMTSPKINRLVQILTGNCLAGISIAWWKWNHNTHHIACNSLEFDPDLQHMPFFAVSSKLFSSLKSYFYERKMNFDSVCRFLVSYQHWTFYPVMCFARLNLFAQSFLLLLSKRRVPNRGQEILGVLVFWIWYPLLVSCLPNWGERVMFVLASFAVTGIQHVQFCLNHFSSNVYVGLPSGNDWFEKQTKGTLNINCSSWMDWFHGGLQFQIEHHLFPRLPRCQLRKISPFVKELCKKHHLPYDSASFWKANVMTIRTLSAAALQARDLANPVPKNLVWEAVNTHG

SNPs (tot: 11)

Position	A %	C %	G %	T %	Probability
1482	20		80		0.995493
1587		16		83	0.994512
1588	16		83		0.994512
1589			83	16	0.994512
1615	20			80	0.995493
1618	75	25			0.996473
1619		25	75		0.996473
1621		25		75	0.996473
1625		33		66	0.997452
1626		33	66		0.997452
1627	33			66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain