SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene10063

Length: 2376 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene10063
TACTTCATGGGATACATCTTCGGAGGCGTGACATAGTGCAAAACATACCGGGCAGCTATAGAATATTTAGCAGGTGCTTTCCGCGTGAGGACCCTTAGGGACAATCGGATGGGCTGCCCTGCCACCTACCTTCCTGCAGTCTGTCTATCTATAAATACAATACCAAGGCCTTTGCTGTAATTTGAATCAATCACACCAGCCTGGTCACTCACCATATCTTCAATCTGTGTGGCAGAGTTGCAGGATTTCGCACTTCAGGCGAAACCATGAATGGAGATTGCAAGCCCAATGCTGCTGCCACTGTTGCAGGAGGCGTCCATGATGTCTATGGCGAAGATAGGGCTTCAGAAGAGCAGGTTATCACTCCTTGGAGCCGAACAGTTGCCAGTGGTTATGTATTGCTGAGAGATCCTCGCTACAACAAAGGTTTGGCATTCACAGATGACGAAAGGGATTATCACTATGTACGAGGTCTTCTACCGCCTGCTCACATGTCACAAGAACTTCAAGTGAAGCGTTTAATGCATCTTATTCGTCAATACAATACACCCTTGCAGAAGTACATGGCTTTGATGGATCTTGAGGAGAGGAACGAAAGGTTATTTTACAAGCTCATGATTGATCATGTCGAAGAGTTGCTTCCTATAGTCTATACACCCACAGTGGGAGAAGCTTGCCAAAAATATGGAACTTATTTTTAGGCGTCCACAGGGCCTTTACATAAGCTTAAAAGAAAAGGGCAAGGTTCATGATGTTTTGAGGAACTGGCCTGAAAAGAACATTAAGGTCATTGTTGTTACTGATGGTGAACGCATTTTGGGACTGGGGGACCTTGGATGCCAGGGTATGGGGATCCCTGTTGGAAAACTAGCACTCTACACGGCACTTGGTGGAATCCGCCCTTCTGAGTGCCTTCCAATAACAATTGACGTGGGGACTAACAATGAGATGCTGCTAAATGATGAGTTCTACATAGGAATGAAGCAGAAGCGTGCTACAGGCCAGGAATATGATGATCTTCTTGATGAGTTTATGTATGCCGCCAAACAAGCTTTTGGAGAGAAAGTCCTTATCCAGTTTGAAGATTTTGCGAATCACAATGCCTTCAAATTGCTTGAAAAGTATAGATCAACACACTTGGTCTTCAATGATGATATTCAGGGAACAGCTTCAGTGGTGCTTGCAGGCCTTGTCGCATCATTAAAATTGACTGGTGGAACATTGGCTGACCACACCTTCCTATTCCTTGGTGCTGGTGAGGCAGGGACAGGTATTGCAGAGCTTATAGCATTGGAAATAACCAAACAGGGTAAAATCTCATTGAATGAAGCTCGCAATAGGATATGGTTGGTGGACTCTAAGGGACTCATTGTGAAGTCAAGAATGGAGTCTCTTCAACCCTTCAAGAAGCCATGGGCACATGAGCATGAACCCTGCAAAGGATTATTGGATGCTGTGAAGTCAATCAGGCCTACAATTTTGATCGGTTCATCGGGGGCTGGAAGAACTTTCACAAAGGAAGTGATTGAAACTGTTTCCTCTTTCAATGAGAAGCCAGTGATTTTGGCCCTTTCTAATCCAACCTCTCAATCTGAATGTACAGCAGAAGAAGCATACACTTGGAGTAAGGGCCGTGCAATATTTGCAAGTGGGAGTCCATTTGATCCTGTCAGTATTGATGGGAAGACCTTTATTCCTGGCCAGTCCAACAATGCCTATATTTTCCCCGGATTGGGTTTAGGCTTGGTAATTTCAGGAACAATTCGTGTGCATGAAGATATGCTATTGGCAGCCTCTGAGGCTTTAGCTGAACAAGTTACCAAAGAGCATCTCGATCAAGGGCTCATTTACCCACCATTTCGAAATATCAGAAAAATATCAGCCCACATAGCAGCAAGAGTTGCTGCAAAATCCTATGAATTAGGTTTGGCAACATACCTTCCTCGTCCTGCTGATCTGGTAGCTTTTGCTGAGAGCTGTATGTACAGTCCAACTTATAGGAATTATAAATGAGATAAAATTTCTCACAGCCCAAACTGGGCAGAGTTTGTTTGACGTTCAATTTAAACTGAAAAGTAGAACTGGGGTAATAAATGAGGAAGAGTCTGCAAAAACTATTTCAAGAATATGCTAATGTCAGTGTTTTGATGGCAATTTTAAAGAATCTACGCCATTGATTTTGTTAATGTAGCAATTCAATTCCTTGTTCATTGGAATGGAGGAACATTACTTGCCATGGTCGGAGGGATGAGAATGGAAATTCTTACTTGAGAGGAACGAAAGTAGAGAGATATCTTTTTTATATGAAACAGACTTCAGAGTTCCTTGAAAAGAAACAACCTTATTTTTAATATAAGTATTTATTCGTTAATGAGT

Ace file of the UniGene sp_v1.1_unigene10063

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Malic enzyme n=1 Tax=Picea sitchensis RepID=B8LRQ8_PICSI	-	-	95%
FL-Next	sp=Malic enzyme; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	86%
Blast2go	protein	-	-	90%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:1.1.1.4-	-	0.0	90%

Source	GOs	Term	Type	Identity
Blast2go	malate metabolic process	GO:0006108	Biological Process	90%
Blast2go	oxidation-reduction process	GO:0055114	Biological Process	90%
Blast2go	metal ion binding	GO:0046872	Molecular Function	90%
Blast2go	NAD binding	GO:0051287	Molecular Function	90%
Blast2go	malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity	GO:0004473	Molecular Function	90%
Blast2go	chloroplast	GO:0009507	Cellular Component	90%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Malic enzyme, N-terminal	IPR012301	-	0.0	90%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LRQ8

Fln msg: Overlapping hits, possible frame ERROR between 743 and 694,

Fln protein: MNGDCKPNAAATVAGGVHDVYGEDRASEEQVITPWSRTVASGYVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQVKRLMHLIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTxxxxxxxxxxxxxxxxxxVHDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPITIDVGTNNEMLLNDEFYIGMKQKRATGQEYDDLLDEFMYAAKQAFGEKVLIQFEDFANHNAFKLLEKYRSTHLVFNDDIQGTASVVLAGLVASLKLTGGTLADHTFLFLGAGEAGTGIAELIALEITKQGKISLNEARNRIWLVDSKGLIVKSRMESLQPFKKPWAHEHEPCKGLLDAVKSIRPTILIGSSGAGRTFTKEVIETVSSFNEKPVILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVSIDGKTFIPGQSNNAYIFPGLGLGLVISGTIRVHEDMLLAASEALAEQVTKEHLDQGLIYPPFRNIRKISAHIAARVAAKSYELGLATYLPRPADLVAFAESCMYSPTYRNYK*

Protein Length: 584

Fln nts: TACTTCATGGGATACATCTTCGGAGGCGTGACATAGTGCAAAACATACCGGGCAGCTATAGAATATTTAGCAGGTGCTTTCCGCGTGAGGACCCTTAGGGACAATCGGATGGGCTGCCCTGCCACCTACCTTCCTGCAGTCTGTCTATCTATAAATACAATACCAAGGCCTTTGCTGTAATTTGAATCAATCACACCAGCCTGGTCACTCACCATATCTTCAATCTGTGTGGCAGAGTTGCAGGATTTCGCACTTCAGGCGAAACC_-_ATGAATGGAGATTGCAAGCCCAATGCTGCTGCCACTGTTGCAGGAGGCGTCCATGATGTCTATGGCGAAGATAGGGCTTCAGAAGAGCAGGTTATCACTCCTTGGAGCCGAACAGTTGCCAGTGGTTATGTATTGCTGAGAGATCCTCGCTACAACAAAGGTTTGGCATTCACAGATGACGAAAGGGATTATCACTATGTACGAGGTCTTCTACCGCCTGCTCACATGTCACAAGAACTTCAAGTGAAGCGTTTAATGCATCTTATTCGTCAATACAATACACCCTTGCAGAAGTACATGGCTTTGATGGATCTTGAGGAGAGGAACGAAAGGTTATTTTACAAGCTCATGATTGATCATGTCGAAGAGTTGCTTCCTATAGTCTATACACCCACAGTGGGAGAAGCTTGCCAAAAATATGGAACTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGTTCATGATGTTTTGAGGAACTGGCCTGAAAAGAACATTAAGGTCATTGTTGTTACTGATGGTGAACGCATTTTGGGACTGGGGGACCTTGGATGCCAGGGTATGGGGATCCCTGTTGGAAAACTAGCACTCTACACGGCACTTGGTGGAATCCGCCCTTCTGAGTGCCTTCCAATAACAATTGACGTGGGGACTAACAATGAGATGCTGCTAAATGATGAGTTCTACATAGGAATGAAGCAGAAGCGTGCTACAGGCCAGGAATATGATGATCTTCTTGATGAGTTTATGTATGCCGCCAAACAAGCTTTTGGAGAGAAAGTCCTTATCCAGTTTGAAGATTTTGCGAATCACAATGCCTTCAAATTGCTTGAAAAGTATAGATCAACACACTTGGTCTTCAATGATGATATTCAGGGAACAGCTTCAGTGGTGCTTGCAGGCCTTGTCGCATCATTAAAATTGACTGGTGGAACATTGGCTGACCACACCTTCCTATTCCTTGGTGCTGGTGAGGCAGGGACAGGTATTGCAGAGCTTATAGCATTGGAAATAACCAAACAGGGTAAAATCTCATTGAATGAAGCTCGCAATAGGATATGGTTGGTGGACTCTAAGGGACTCATTGTGAAGTCAAGAATGGAGTCTCTTCAACCCTTCAAGAAGCCATGGGCACATGAGCATGAACCCTGCAAAGGATTATTGGATGCTGTGAAGTCAATCAGGCCTACAATTTTGATCGGTTCATCGGGGGCTGGAAGAACTTTCACAAAGGAAGTGATTGAAACTGTTTCCTCTTTCAATGAGAAGCCAGTGATTTTGGCCCTTTCTAATCCAACCTCTCAATCTGAATGTACAGCAGAAGAAGCATACACTTGGAGTAAGGGCCGTGCAATATTTGCAAGTGGGAGTCCATTTGATCCTGTCAGTATTGATGGGAAGACCTTTATTCCTGGCCAGTCCAACAATGCCTATATTTTCCCCGGATTGGGTTTAGGCTTGGTAATTTCAGGAACAATTCGTGTGCATGAAGATATGCTATTGGCAGCCTCTGAGGCTTTAGCTGAACAAGTTACCAAAGAGCATCTCGATCAAGGGCTCATTTACCCACCATTTCGAAATATCAGAAAAATATCAGCCCACATAGCAGCAAGAGTTGCTGCAAAATCCTATGAATTAGGTTTGGCAACATACCTTCCTCGTCCTGCTGATCTGGTAGCTTTTGCTGAGAGCTGTATGTACAGTCCAACTTATAGGAATTATAAATGA___GATAAAATTTCTCACAGCCCAAACTGGGCAGAGTTTGTTTGACGTTCAATTTAAACTGAAAAGTAGAACTGGGGTAATAAATGAGGAAGAGTCTGCAAAAACTATTTCAAGAATATGCTAATGTCAGTGTTTTGATGGCAATTTTAAAGAATCTACGCCATTGATTTTGTTAATGTAGCAATTCAATTCCTTGTTCATTGGAATGGAGGAACATTACTTGCCATGGTCGGAGGGATGAGAATGGAAATTCTTACTTGAGAGGAACGAAAGTAGAGAGATATCTTTTTTATATGAAACAGACTTCAGAGTTCCTTGAAAAGAAACAACCTTATTTTTAATATAAGTATTTATTCGTTAATGAGT

Fln Alignment:

sp_v1.1_unigene10063___MNGDCKPNAAATVAGGVHDVYGEDRASEEQVITPWSRTVASGYVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQVKRLMHLIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTxxxxxxxxxxxxxxxxxVHDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPITIDVGTNNEMLLNDEFYIGMKQKRATGQEYDDLLDEFMYAAKQAFGEKVLIQFEDFANHNAFKLLEKYRSTHLVFNDDIQGTASVVLAGLVASLKLTGGTLADHTFLFLGAGEAGTGIAELIALEITKQGKISLNEARNRIWLVDSKGLIVKSRMESLQPFKKPWAHEHEPCKGLLDAVKSIRPTILIGSSGAGRTFTKEVIETVSSFNEKPVILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVSIDGKTFIPGQSNNAYIFPGLGLGLVISGTIRVHEDMLLAASEALAEQVTKEHLDQGLIYPPFRNIRKISAHIAARVAAKSYELGLATYLPRPADLVAFAESCMYSPTYRNYK
B8LRQ8_______________MNGDSKSTGAATLAGGVHDVYGEDRASEEQIVTPWSRTVASGNVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQEKRLMHIIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTxxxxxxxxxxxxxxxxxVYDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPITIDVGTNNEKLLNDEFYIGLRQKRATGQEYDDLLNEFMYAAKKAYGEKVLIQFEDFANHNAFNLLAKYRSTHLVFNDDIQGTASVVLSGLVASLKLTGGTLADHTFLFLGAGEAGTGIAELIALEMTKQGKISLNEARKRIWLVDSKGLIVKSRMESLQHFKKPWAHEHEPCKGLLDAVKSIRPTILIGSSGVGRTFTKEVIETVSSFNEKPVILALSNPTSQSECTAEEAYTWSKGRAIFASGSPFDPVSINGKTFIPGQSNNAYIFPGLGLGLVISGTIRVHEDMLLAASEALAQQVSKEHLEQGLIYPPFRNIRKISAHIAAGVAAKSYELGLATRLPRPADLVAFAESCMYSPVYRNYK

SNPs (tot: 27)

Position	A %	C %	G %	T %	Probability
143		25		75	0.996473
181	90			10	0.90298
185			30	70	1.0
186			30	70	1.0
474		11	88		0.923564
478			11	88	0.923564
481			11	88	0.923564
486		88	11		0.923564
491	11			88	0.923564
493	88			11	0.923564
495	88		11		0.923564
508			10	90	0.90298
511	90		10		0.90298
513		10	90		0.90298
514		10		90	0.90298
515			88	11	0.923564
516	88			11	0.923564
517	88		11		0.923564
520			88	11	0.923564
521			12	87	0.992554
523		12		87	0.992554
525	75	12		12	0.975346
527		12	87		0.992554
528	12	87			0.992554
1345			80	20	0.999996
1657			25	75	0.996473
2346	60		40		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain