SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene9611

Length: 2290 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene9611
CGTCGTGACCTTAACCAGCACACACTTGGATTTAAATGAAAAGATCTCAACAAGCAGATTTTCGGATTTAAATGAAAAGATCCTAGAAGCAGAGTCTTGGATTTAAATTAAAAGATCTTAACCAGCAGAGTCTTGCAATTTATAGATCTGAGGGAAGAATTGACGGAATCACGATGAAGCCGTTTGTGGTTCTGGTGCTCCTGCTGCACTGTGCTTGCGTTCTCAGCGAACGGAGATTTCCGACGGAAATCGATCGCGGGGCAAAAGCGGCGTCCGGCGGAGTTGACAGTGTGGCTTCTTCTATGGGATTCATTGTGAAGCCTACTTCGGAGAATTGGCCGCGGCGGGATTCGAACTCGAACCTGAAGGCTAATAGTTCGTCTACGTGTGAGGAAAGCTATGGATTCTTGCCCTGCACCGAGTCTGCGTTGGGGAATCTGTTCCTGCTCGTGGTTTACGGATACATAATGTTCGTGGCGGCGTCTCTTCTATCGGATGGAAGTGAGCTTCTGTTGGTTGTGATGGGGCCTGGGATCATCGGAGGCTTGTTTCTGCCTGTGCTCGGAGCTTTTCCAGATGCCCTTCTCATACTAGTGTCTGGTCTATCTGGAAGTAAGACAACTGCGCAATCACAAGTGCTGATTGGAATGGGGTTGCTTGCTGGTTCAACTGTAATGTTATTGACAGCATTGTGGGGGGCATGTACGATTGTCGGCAAATGTGATCTTTCTGAAGATAATGTTGCCATTGATGGGAAGGATACAAAAAGATTCAGCCTTTTTGGTTCAGGTATTACTACAGATAAAGCAACTACTAAGGCTGCACAAATTATGGTGGTGTCTCTCATACCATTCATTATTGTAGAGATCCCTCAAGCTATTGACTCATCTTCGGCTAAACGAATAGGGGTTATGGTAGGATGTATTGCTGCAATTATATGCTTACTATCATATTGTACATATCAGATCTTACAACCATGGATTCAGAAAAGAAGAGTGGCATATGCGAAGCAAAAACATATAATATCAGCTATCATGAAAGCTGTGCATTTGAAAGGATGGGGAAGGCTCCTCACAGAAGATGGCCAACCTGATCAGGATGTCATTAAAAGTGTATTCGAAAAGCTTGACAATGACAATGATGGATCTTTGACAAAACATGAATTACGTGGGCTTTTATATGGGCTTGAATTGCAAGGTCCAGATTCTTCAACAAATGATGCCGTTAACAAAGTAATGGCAGAATTTGATTCCTCGAGAGATGACAAGCTTAGCCTAGAAGAGTTCAAAGTTGGAATGAGTTCATGGCTTAGGATTGCAATCTCCACAGTTGGTCATCCAAAACCAAATAACTACTCAAAGAAATTTATATCTGATTTTCAATCGAAAACAAAGGAGGAACTTGAGGCTCTTCAGAATGAGAATGAGGAGGAAGTTGCTGAAGTTGATAATCCATACAAAGTTTATCTGAAGGCAGTAATGCTGATGCTGGGAGGTGCCATTTTAGCGGGCATATTTGCAGATCCCCTTGTAGATGCTGTTGACAATTTTTCTGATGCAACAAGCATCCCTTCATTCTTCATCTCATTCATAATAATGCCTTTGGCTACAAACTCCAGTGAGGGTGTTTCAGCAATTATTTTTGCAAGCCGGAAGAAAAAGCGCACTGCTTCTTTGACTTATTCTGAGATCTATGGTGCAGTGACCATGAACAACACGTTATGTTTGGGAGTCTTCTTGGCCATTATTTATTTCCGTGGCTTGGATTGGGACTTCTCAGCTGAGGTGCTAGTAATTGTACTTGTTGTTTTAGTGATGGGCCTTTTAAGCAGCTTTCGTACCACTTTCCCTCTGTGGATGAGTTCGATTGCCTTCATTTTTTATCCCTTAACGTTAGCCATAGTTTACGTCCTAGATTACGTACTAGGATGGTCATAAAGAGAGTACAGATGGTTTTCGCATGTCGTGTCATAGATAAAATTTTGTATAGAAGTACATCAGTTTGTCTTGGGTGCGAAAATGTTTTCTTTTGCAATGGTATTTATGTCAATAAGTGTAATCTTATATGGTTATATGCACAAAGAAGTTGTATGTTCCAAAACAATGAACTCGTTGGATTGAACGTAGTCCACGGAGTTGTACAATTTTACTGAATATTACTTGTCCACTGGCCCTATCTTGACAGCCTACGTCTATGCAATGAGCTTTCATTTATTTTGTTCGTACCAAATTTTTGGGATGTGTTTCCATACAATGATGGCCAGTAACCTATTTGACCTGAATCAATGAAA

Ace file of the UniGene sp_v1.1_unigene9611

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	sodium/calcium exchanger family protein / calcium-binding EF hand family protein	-	-	50%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	87%
Blast2go	sodium calcium exchanger family protein	-	-	74%

Source	GOs	Term	Type	Identity
Blast2go	plant-type vacuole	GO:0000325	Cellular Component	74%
Blast2go	plasma membrane	GO:0005886	Cellular Component	74%
Blast2go	vacuolar membrane	GO:0005774	Cellular Component	74%

Source	InterPros	Term	Type	Identity
Blast2go	Calcium-binding EF-hand	IPR002048	-	74%
Blast2go	Sodium/calcium exchanger membrane region	IPR004837	-	74%
Blast2go	EF-hand-like domain	IPR011992	-	74%
Blast2go	EF-Hand 1, calcium-binding site	IPR018247	-	74%
Blast2go	EF-HAND 2	IPR018249	-	74%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5A885

Fln msg: your sequence is longer than subject: 588 - 233

Fln protein: MKPFVVLVLLLHCACVLSERRFPTEIDRGAKAASGGVDSVASSMGFIVKPTSENWPRRDSNSNLKANSSSTCEESYGFLPCTESALGNLFLLVVYGYIMFVAASLLSDGSELLLVVMGPGIIGGLFLPVLGAFPDALLILVSGLSGSKTTAQSQVLIGMGLLAGSTVMLLTALWGACTIVGKCDLSEDNVAIDGKDTKRFSLFGSGITTDKATTKAAQIMVVSLIPFIIVEIPQAIDSSSAKRIGVMVGCIAAIICLLSYCTYQILQPWIQKRRVAYAKQKHIISAIMKAVHLKGWGRLLTEDGQPDQDVIKSVFEKLDNDNDGSLTKHELRGLLYGLELQGPDSSTNDAVNKVMAEFDSSRDDKLSLEEFKVGMSSWLRIAISTVGHPKPNNYSKKFISDFQSKTKEELEALQNENEEEVAEVDNPYKVYLKAVMLMLGGAILAGIFADPLVDAVDNFSDATSIPSFFISFIIMPLATNSSEGVSAIIFASRKKKRTASLTYSEIYGAVTMNNTLCLGVFLAIIYFRGLDWDFSAEVLVIVLVVLVMGLLSSFRTTFPLWMSSIAFIFYPLTLAIVYVLDYVLGWS*

Protein Length: 589

Fln nts: CGTCGTGACCTTAACCAGCACACACTTGGATTTAAATGAAAAGATCTCAACAAGCAGATTTTCGGATTTAAATGAAAAGATCCTAGAAGCAGAGTCTTGGATTTAAATTAAAAGATCTTAACCAGCAGAGTCTTGCAATTTATAGATCTGAGGGAAGAATTGACGGAATCACG_-_ATGAAGCCGTTTGTGGTTCTGGTGCTCCTGCTGCACTGTGCTTGCGTTCTCAGCGAACGGAGATTTCCGACGGAAATCGATCGCGGGGCAAAAGCGGCGTCCGGCGGAGTTGACAGTGTGGCTTCTTCTATGGGATTCATTGTGAAGCCTACTTCGGAGAATTGGCCGCGGCGGGATTCGAACTCGAACCTGAAGGCTAATAGTTCGTCTACGTGTGAGGAAAGCTATGGATTCTTGCCCTGCACCGAGTCTGCGTTGGGGAATCTGTTCCTGCTCGTGGTTTACGGATACATAATGTTCGTGGCGGCGTCTCTTCTATCGGATGGAAGTGAGCTTCTGTTGGTTGTGATGGGGCCTGGGATCATCGGAGGCTTGTTTCTGCCTGTGCTCGGAGCTTTTCCAGATGCCCTTCTCATACTAGTGTCTGGTCTATCTGGAAGTAAGACAACTGCGCAATCACAAGTGCTGATTGGAATGGGGTTGCTTGCTGGTTCAACTGTAATGTTATTGACAGCATTGTGGGGGGCATGTACGATTGTCGGCAAATGTGATCTTTCTGAAGATAATGTTGCCATTGATGGGAAGGATACAAAAAGATTCAGCCTTTTTGGTTCAGGTATTACTACAGATAAAGCAACTACTAAGGCTGCACAAATTATGGTGGTGTCTCTCATACCATTCATTATTGTAGAGATCCCTCAAGCTATTGACTCATCTTCGGCTAAACGAATAGGGGTTATGGTAGGATGTATTGCTGCAATTATATGCTTACTATCATATTGTACATATCAGATCTTACAACCATGGATTCAGAAAAGAAGAGTGGCATATGCGAAGCAAAAACATATAATATCAGCTATCATGAAAGCTGTGCATTTGAAAGGATGGGGAAGGCTCCTCACAGAAGATGGCCAACCTGATCAGGATGTCATTAAAAGTGTATTCGAAAAGCTTGACAATGACAATGATGGATCTTTGACAAAACATGAATTACGTGGGCTTTTATATGGGCTTGAATTGCAAGGTCCAGATTCTTCAACAAATGATGCCGTTAACAAAGTAATGGCAGAATTTGATTCCTCGAGAGATGACAAGCTTAGCCTAGAAGAGTTCAAAGTTGGAATGAGTTCATGGCTTAGGATTGCAATCTCCACAGTTGGTCATCCAAAACCAAATAACTACTCAAAGAAATTTATATCTGATTTTCAATCGAAAACAAAGGAGGAACTTGAGGCTCTTCAGAATGAGAATGAGGAGGAAGTTGCTGAAGTTGATAATCCATACAAAGTTTATCTGAAGGCAGTAATGCTGATGCTGGGAGGTGCCATTTTAGCGGGCATATTTGCAGATCCCCTTGTAGATGCTGTTGACAATTTTTCTGATGCAACAAGCATCCCTTCATTCTTCATCTCATTCATAATAATGCCTTTGGCTACAAACTCCAGTGAGGGTGTTTCAGCAATTATTTTTGCAAGCCGGAAGAAAAAGCGCACTGCTTCTTTGACTTATTCTGAGATCTATGGTGCAGTGACCATGAACAACACGTTATGTTTGGGAGTCTTCTTGGCCATTATTTATTTCCGTGGCTTGGATTGGGACTTCTCAGCTGAGGTGCTAGTAATTGTACTTGTTGTTTTAGTGATGGGCCTTTTAAGCAGCTTTCGTACCACTTTCCCTCTGTGGATGAGTTCGATTGCCTTCATTTTTTATCCCTTAACGTTAGCCATAGTTTACGTCCTAGATTACGTACTAGGATGGTCATAA___AGAGAGTACAGATGGTTTTCGCATGTCGTGTCATAGATAAAATTTTGTATAGAAGTACATCAGTTTGTCTTGGGTGCGAAAATGTTTTCTTTTGCAATGGTATTTATGTCAATAAGTGTAATCTTATATGGTTATATGCACAAAGAAGTTGTATGTTCCAAAACAATGAACTCGTTGGATTGAACGTAGTCCACGGAGTTGTACAATTTTACTGAATATTACTTGTCCACTGGCCCTATCTTGACAGCCTACGTCTATGCAATGAGCTTTCATTTATTTTGTTCGTACCAAATTTTTGGGATGTGTTTCCATACAATGATGGCCAGTAACCTATTTGACCTGAATCAATGAAA

Fln Alignment:

sp_v1.1_unigene9611___MAEFDSSRDDKLSLEEFKVGMSSWLRIAISTVGHPKPNNYSKKFISDFQSKTKXXXXXXXXXXXXXXXXXDNPYKVYLKAVMLMLGGAILAGIFADPLVDAVDNFSDATXXXXXXXXXXXMPLATNSSEGVSAIIFASRKKKRTASLTYSEIYGAVTMNNTLCLGVFLAIIYFRGLDWDFSAEXXXXXXXXXXXXXXSSFRTTFPLWMSSIAFIFYPLTLAIVYVLDYVLGWS
D5A885______________MAEFDSSRDEKLSLEEFKAGMSSWLKIATSTVGRSKSNNYSRKFLSSFQSRSKEEVEALENADEEDVTEVDNRKKIYLKAVMLMLGGAIIAGIFADPLVAAVDNFSDATSIPSFFISFIIMPLATNSSEGVSALIFASRKKKRTASLTFSEIYGAVTMNNTLCLGVFLAIIYIRGLDWDFSAEVLVIVLVVLVMGLLGSFRTTFPLWMSFIAFLFYPLTLAIVYVLDYVLGWS

SNPs (tot: 32)

Position	A %	C %	G %	T %	Probability
228		83		16	0.994512
273	16		83		0.994512
287	83		16		0.994512
332	90	10			0.90298
334	90			10	0.90298
335	90	10			0.90298
336			10	90	0.90298
339	10		90		0.90298
342			90	10	0.90298
343		90		10	0.90298
347			90	10	0.90298
349			90	10	0.90298
351		10		90	0.90298
354	10		90		0.90298
526	10		90		0.90298
706	90			10	0.90298
708		10	90		0.90298
713	10			90	0.90298
714		90		10	0.90298
778		88		11	0.923564
1200		10		90	0.90298
1854	9		90		0.999842
1857	9		86	4	0.999642
1858	90	9			0.999842
1861	90		9		0.999842
1862		9	85		0.999726
2160		57	42		1.0
2161		6		93	0.999998
2185	8		91		1.0
2186	5	94			0.998894
2211	18			9	0.999994
2213	20			80	0.999996

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain