SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene9237

Length: 1600 nt

Origin of reads: Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene9237 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	ribosomal protein S6 kinase, putative (EC:2.7.11.13); K04688 p70 ribosomal S6 kinase [EC:2.7.11.1]	-	-	72%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	77%
Blast2go	protein	-	-	85%

Source	ECs	Term	Type	e value	Identity
AutoFact	Non-specific serine/threonine protein kinase.	EC:2.7.11.1	-	0.0	72%

Source	InterPros	Term	Type	Identity
Blast2go	Protein kinase, catalytic domain	IPR000719	-	85%
Blast2go	Serine/threonine- / dual-specificity protein kinase, catalytic domain	IPR002290	-	85%
Blast2go	Serine/threonine-protein kinase, active site	IPR008271	-	85%
Blast2go	Protein kinase-like domain	IPR011009	-	85%
Blast2go	Protein kinase, ATP binding site	IPR017441	-	85%
Blast2go	Serine/threonine-protein kinase-like domain	IPR017442	-	85%
Blast2go	Tyrosine-protein kinase, catalytic domain	IPR020635	-	85%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9P2E8

Fln msg: Overlapping hits, possible frame ERROR between 1315 and 1308, your sequence is longer than subject: 432 - 192

Fln protein: MVVPICSSLFSEYLLLWCIYSKMVFAGVSGALRGDKCKSFVLRFPESNGQLGYEEIEFSDVFGPVVASSENFCVIPSVQDLQTINRQGRAPKVKVTQNIGVDDFELLKVVGQGAFGKVFQVKRIGTSEIYAMKVMTKDRIMEKNHAEYIKAERDIMTRIEHPFIVQLRYSFQTKYKLYLVLDFINGGHLLFQLRRQRLFSEDLARVYVAEIISAVSYLHQNGIVHRDLKPENILLDSEGHVKLTDFGLAKEVDESGRSNSMCGTPEYMAPEIIQAKGHDKAVDWWSVGILLYEMLTGQRPFTGNNRQKVQNKIIKDKVKLPPFLTSEAHSLLKGLLQKDLSKRLGSGASGSDDIKGHKWxxxxISWKKLEARQIQPAFKPDVKGNCCTANFDCFWTNMPLDDNEPPSTPKFNNDQHHFTNYSYIQLNDTKH*

Protein Length: 433

Fln nts: CCCAAGTTTGGAATGTGCAGACACCTCTGGGCTATTCCAAACTTGGGAAGCCAATACGTAAATCCATCGCCTCTCCTGCACTTTTCCCAGGTTTAGGTCGAAGTGTACCATATGAGTTCAAGCTACACTGTTGCATATGGTCTTTCCTGTAACAGGAAAGACAGGGGCAGGTTCTGTCTTTGGATATTGACCTGCCTTTTCCCACGAATTCCTGAGTTTGCATTGGTGT_-_ATGGTAGTGCCCATTTGCTCAAGTCTGTTCTCAGAGTACCTTCTTCTGTGGTGTATCTATAGCAAAATGGTTTTTGCAGGGGTATCTGGTGCTCTCAGAGGTGACAAGTGCAAGTCTTTCGTTTTAAGATTTCCAGAATCCAATGGACAACTGGGATATGAGGAGATTGAATTTTCAGATGTATTTGGTCCTGTTGTTGCTTCATCAGAGAATTTCTGTGTAATTCCTTCGGTTCAGGATCTTCAAACCATAAATCGACAGGGCAGAGCACCGAAAGTCAAGGTTACTCAAAACATTGGAGTTGACGATTTTGAGCTGCTTAAGGTGGTTGGACAAGGTGCATTTGGTAAGGTGTTCCAGGTTAAAAGAATTGGAACATCGGAGATTTATGCTATGAAAGTTATGACGAAAGACAGAATTATGGAAAAGAATCATGCGGAATACATAAAAGCTGAAAGAGACATTATGACAAGGATTGAGCATCCCTTTATTGTTCAGCTCAGATACTCCTTCCAGACAAAATATAAACTTTATCTTGTATTGGATTTTATAAATGGTGGGCATCTCCTCTTTCAGCTTCGACGACAAAGGCTCTTCAGTGAGGATTTAGCTCGGGTTTACGTTGCGGAAATAATTTCTGCTGTCTCATACCTTCATCAAAATGGGATCGTTCACCGGGATCTTAAACCTGAGAACATTCTTCTAGACTCTGAAGGCCATGTTAAGTTGACTGATTTTGGGCTAGCAAAGGAAGTAGATGAGTCAGGTCGATCCAATAGTATGTGTGGGACTCCAGAATATATGGCACCTGAAATTATTCAAGCTAAAGGCCATGATAAAGCTGTTGATTGGTGGAGTGTTGGAATTCTACTGTATGAGATGCTCACTGGACAGCGTCCCTTCACTGGTAATAACCGCCAGAAGGTACAAAATAAAATCATCAAGGATAAAGTTAAATTGCCACCATTTTTGACCAGTGAAGCCCATTCATTGCTGAAAGGCCTGCTTCAGAAGGATCTAAGCAAGCGGCTAGGCAGTGGAGCAAGTGGGAGTGATGACATCAAAGGACACAAGTGGxxxxxxxxxxxxATTAGCTGGAAGAAACTGGAAGCCAGGCAGATACAGCCAGCATTTAAGCCTGATGTTAAGGGGAATTGTTGCACCGCTAATTTTGATTGTTTCTGGACCAATATGCCTCTGGATGATAATGAACCACCTTCAACTCCGAAATTTAACAATGATCAACATCATTTCACAAACTACTCATATATCCAACTCAATGACACGAAGCATTAG___CCTTCTTTTCCATATAAGGAATCCGCATCCAGCGTTGAAGAAACTAAAATCATTGGGCTTAAATTATAGTTCTGCCA

Fln Alignment:

sp_v1.1_unigene9237___LTDFGLAKEVDESGRSNSMCGTPEYMAPEIIQAKGHDKAVDWWSVGILLYEMLTGQRPFTGNNRQKVQNKIIKDKVKLPPFLTSEAHSLLKGLLQKDLSKRLGSGASGSDDIKGHKWxxxISWKKLEARQIQPAFKPDVKGNCCTANFDCFWTNMPLDDNEPPSTPKFNNDQHHFTNYSYIQLNDTKH*PSFPYKES
A9P2E8______________LTDFGLAKEVDESMRSNSMCGTTEYMAPEILLSKGHNKSADWWSVGILLYEMLTGQPPYTNNNRHKLQEKIIKEKIKLPSYLTSEVHSLLKGLLQKDPSKRIGSGANGSDEIKRHKWxxxINWRKLEAREIQPNFRPNVSGKDCIANFDERWTAMPPQDS-PASTPK---DGAYFQGYTYVAPNPWL---SEPNKES

SNPs (tot: 60)

Position	A %	C %	G %	T %	Probability
144	50	50			1.0
184	50		50		1.0
271		16		83	0.994512
283	83	16			0.994512
301		16	83		0.994512
304		12		75	0.975346
307			14	85	0.993533
308	14			85	0.993533
309		14		85	0.993533
313			66	33	1.0
334	60		40		1.0
364	40	20		40	1.0
398		40	60		1.0
432		40		60	1.0
437		25		75	0.996473
444		25	75		0.996473
450			20	80	0.995493
451	20	80			0.995493
452	20			80	0.995493
456			80	20	0.995493
460	60		40		1.0
476		40	60		1.0
478		40		60	1.0
480			80	20	0.995493
484	50			50	1.0
485			50	50	1.0
499		50	50		1.0
502	25		25	50	0.995533
523	50		50		1.0
562	40		60		1.0
579			16	83	0.994512
668	75			25	0.996473
703	50		50		1.0
750		66		33	1.0
783		44		55	1.0
791		16		83	0.994512
792		50	50		1.0
796		16		83	0.994512
804	60		40		1.0
859	16		83		0.994512
861	83			16	0.994512
862	16		83		0.994512
863		16	83		0.994512
893	33			66	1.0
913		33		66	1.0
927		16		83	0.994512
931		33		66	1.0
934	16	66	16		0.988813
938		16		83	0.994512
944			16	83	0.994512
948		83		16	0.994512
951		16		83	0.994512
954			16	83	0.994512
965		33	66		1.0
991		66		33	1.0
1001	66			33	1.0
1057		33		66	0.997452
1068		33		66	0.997452
1251		66		33	0.997452
1413	66		33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain