SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene8405

Length: 2163 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene8405
ATGGGGATCTCATTCTTATTCATGGTGATCGAGGGAAGTTTATTGTCGAAACAGTTGTTATAGCTTTGTTTGGGTGAGAAAACTATTATAATTCGTCAATGCAACAAAGACGAAATCCTAATTTGATATCACCGATATCAATCTAGCTCGTTTTCCTACAGATCTAGTCTGATCGGTACCGATACTGATTTCCCTGCAGATCTAGTCTCATCAGTTCTGAAATTTACTAATACGGAAAGTTAAGACGCCGTTTTATATTTTGGGTATATTGAAAGTAGTTTTCTGATCATAGATATGGCACTGAAGAAGGCGTCGAAGCCGGTTTTCCGACTCTTCTCAGCGGAATGCAATGCTCGGCGGACATTCTTTCGGTCGTTGGCGAATTCAGTGGATCAGGGTTTGCAGAGAGATTGCCTTCACCAATCAGGATCTTCGAAATCTCACTGGGGTTCGAATTCGGGGACTTATATGAGGTGGGCTTCGGCGCTCGTCCTCAGTGCTGGTTTGGGTTTACTGCAATTTCCGGAATTCAAAAGTGATTTTAGTAATTCAAACGAAGATCAACGAACGGAAGGACGCACAAACAAATTTATTCTTGGAGATTCATACAGGAGACGACTATTTTTCAACTACGAGAGGAGAATAAGGATGCGAAGTTCTCCTGAAAAGGTATTTGAATACTTTGCATCATTTAGAACTCCTGATGGAGAGTTATTTATGACTCCTGCTGACCTGGTGCGAGCAGTAGTTCCTGTATTTCCACCACCAGATTCTAATATTGTCAGGGATGGATCTTTGAGAGGGGAACGATCTCCTGGAGAACTTCATTGCCCTCAATCAGAGTTATTCATGCGGTTTGATACTAATGGGGATGGCCTTATCTCCTTTCCAGAGTATATTTTTTTCGTAACACTCTTGAGTATTCCTGAATCGGATATTTTAGCTACGATCAAAATGTTTGATCTGGACCGCAATGGGGAGATGGAGAAAGAAGAATTCAAGAAAGTAATGAAATTTTTGCGAGCTAATAACAGGCAAGGGTCTGCTCAACGTGATGGCCTTCGTATAGGACTTAAAGTCGACAAATCTGTGGATAATGGAGGTCTTGTGGAGTATTTTTTTGGTAAAGGACGGGAAGGACCGTCTTCAGCTTATGAACTTTGCACAGTTTGTCAAGGATTTGCACAAGGAAATTACACGTCTAGAGTTTTTGCACTATGATCATCAGGGATGTGGTAGCATATCAGCCAGAGATTTTGCGTTGTCGATGGTTGCTGCAGCAGATGTGATATGTGTAAATCGGTACTTGGATCGGGTAGATGATCTCAGCAAGGACCCCAGTCTGAGTAATATACACATTACACGTGAGGAATTTGAGAACTTTGCTGAGTTGCGCAAGAAACTGAAGCCTCTATCATTGGCCATATCCAGTTATGGAAAAGTTTATGGAATCTTGACCAAGTCTGATTTTCGACGAGCAGCATCTCATGTTTGTGACGTTGACGTCACAGGCAACATGGTTGATATCATATTTTATATTTTTGACAAAAATAACGATGGCAACCTTAGCACGGATGAATTTTTGGCAGTATTAGAGCGGCGTGAAAAAGATATTGCATACCCTATAGAGTCTGGGATTATTCACTCGCTGACTTCTTGGTGGAAATGTGCGAAAGATTGTCGTTCGGCATGGAATTATTAGTGATTTGAAGAACCATTCATTATTATCCCGTGCAAATCAAGGAATTTTACAATGAGGTTTAATGAGACTCCACAATTGCAGCAGGTGCAGGACTTGTGACAGCTACTTGTGTACATTGGAAGGATCAAGCAAATCATATTATCATTGGAAAATCCAGTCATGGTGTCTGGCAATCCAAGAAATTGCCTTCAAGCCCTAGTGACTTAGGCTGGAGGCAGAAAGACCAGCAATGGCTCTTTTCAAATGGTCTTTCGGGAAGTCTTCATTATGGCATCTAACAATTCACATGCTTTCACATTGCCAATTATTTAGAATGCCTAGTGGGTTTAATCGGAGTTCTTGGAGATGACTTGTCGAGTACTGGAAACNGTCTCATGTAAAATATCAATTTTTTGATGTCATCTTTAAACGTAAAAAAACTAATATGTGCTCGCTAGTTAAGAGTGTAACTTAATATCACA

Ace file of the UniGene sp_v1.1_unigene8405 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	calcium ion binding protein, putative	-	-	66%
FL-Next	tr=Os02g0613000 protein; Oryza sativa subsp. japonica (Rice).	-	-	71%
Blast2go	calcium-binding ef hand family protein	-	-	78%

Source	InterPros	Term	Type	Identity
Blast2go	Calcium-binding EF-hand	IPR002048	-	78%
Blast2go	EF-hand-like domain	IPR011992	-	78%
Blast2go	EF-Hand 1, calcium-binding site	IPR018247	-	78%
Blast2go	EF-HAND 2	IPR018249	-	78%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: tr_plants

Fln subject: Q0DZK7

Fln msg: Overlapping hits, possible frame ERROR between 1128 and 1118,

Fln protein: MALKKASKPVFRLFSAECNARRTFFRSLANSVDQGLQRDCLHQSGSSKSHWGSNSGTYMRWASALVLSAGLGLLQFPEFKSDFSNSNEDQRTEGRTNKFILGDSYRRRLFFNYERRIRMRSSPEKVFEYFASFRTPDGELFMTPADLVRAVVPVFPPPDSNIVRDGSLRGERSPGELHCPQSELFMRFDTNGDGLISFPEYIFFVTLLSIPESDILATIKMFDLDRNGEMEKEEFKKVMKFLRANNRQGSAQRDGLRIGLKVDKSVDNGGLVEYxxxxxDGKDRLQLMNFAQFVKDLHKEITRLEFLHYDHQGCGSISARDFALSMVAAADVICVNRYLDRVDDLSKDPSLSNIHITREEFENFAELRKKLKPLSLAISSYGKVYGILTKSDFRRAASHVCDVDVTGNMVDIIFYIFDKNNDGNLSTDEFLAVLERREKDIAYPIESGIIHSLTSWWKCAKDCRSAWNY*

Protein Length: 471

Fln nts: ATGGGGATCTCATTCTTATTCATGGTGATCGAGGGAAGTTTATTGTCGAAACAGTTGTTATAGCTTTGTTTGGGTGAGAAAACTATTATAATTCGTCAATGCAACAAAGACGAAATCCTAATTTGATATCACCGATATCAATCTAGCTCGTTTTCCTACAGATCTAGTCTGATCGGTACCGATACTGATTTCCCTGCAGATCTAGTCTCATCAGTTCTGAAATTTACTAATACGGAAAGTTAAGACGCCGTTTTATATTTTGGGTATATTGAAAGTAGTTTTCTGATCATAGAT_-_ATGGCACTGAAGAAGGCGTCGAAGCCGGTTTTCCGACTCTTCTCAGCGGAATGCAATGCTCGGCGGACATTCTTTCGGTCGTTGGCGAATTCAGTGGATCAGGGTTTGCAGAGAGATTGCCTTCACCAATCAGGATCTTCGAAATCTCACTGGGGTTCGAATTCGGGGACTTATATGAGGTGGGCTTCGGCGCTCGTCCTCAGTGCTGGTTTGGGTTTACTGCAATTTCCGGAATTCAAAAGTGATTTTAGTAATTCAAACGAAGATCAACGAACGGAAGGACGCACAAACAAATTTATTCTTGGAGATTCATACAGGAGACGACTATTTTTCAACTACGAGAGGAGAATAAGGATGCGAAGTTCTCCTGAAAAGGTATTTGAATACTTTGCATCATTTAGAACTCCTGATGGAGAGTTATTTATGACTCCTGCTGACCTGGTGCGAGCAGTAGTTCCTGTATTTCCACCACCAGATTCTAATATTGTCAGGGATGGATCTTTGAGAGGGGAACGATCTCCTGGAGAACTTCATTGCCCTCAATCAGAGTTATTCATGCGGTTTGATACTAATGGGGATGGCCTTATCTCCTTTCCAGAGTATATTTTTTTCGTAACACTCTTGAGTATTCCTGAATCGGATATTTTAGCTACGATCAAAATGTTTGATCTGGACCGCAATGGGGAGATGGAGAAAGAAGAATTCAAGAAAGTAATGAAATTTTTGCGAGCTAATAACAGGCAAGGGTCTGCTCAACGTGATGGCCTTCGTATAGGACTTAAAGTCGACAAATCTGTGGATAATGGAGGTCTTGTGGAGTATxxxxxxxxxxxxxxxGACGGGAAGGACCGTCTTCAGCTTATGAACTTTGCACAGTTTGTCAAGGATTTGCACAAGGAAATTACACGTCTAGAGTTTTTGCACTATGATCATCAGGGATGTGGTAGCATATCAGCCAGAGATTTTGCGTTGTCGATGGTTGCTGCAGCAGATGTGATATGTGTAAATCGGTACTTGGATCGGGTAGATGATCTCAGCAAGGACCCCAGTCTGAGTAATATACACATTACACGTGAGGAATTTGAGAACTTTGCTGAGTTGCGCAAGAAACTGAAGCCTCTATCATTGGCCATATCCAGTTATGGAAAAGTTTATGGAATCTTGACCAAGTCTGATTTTCGACGAGCAGCATCTCATGTTTGTGACGTTGACGTCACAGGCAACATGGTTGATATCATATTTTATATTTTTGACAAAAATAACGATGGCAACCTTAGCACGGATGAATTTTTGGCAGTATTAGAGCGGCGTGAAAAAGATATTGCATACCCTATAGAGTCTGGGATTATTCACTCGCTGACTTCTTGGTGGAAATGTGCGAAAGATTGTCGTTCGGCATGGAATTATTAG___TGATTTGAAGAACCATTCATTATTATCCCGTGCAAATCAAGGAATTTTACAATGAGGTTTAATGAGACTCCACAATTGCAGCAGGTGCAGGACTTGTGACAGCTACTTGTGTACATTGGAAGGATCAAGCAAATCATATTATCATTGGAAAATCCAGTCATGGTGTCTGGCAATCCAAGAAATTGCCTTCAAGCCCTAGTGACTTAGGCTGGAGGCAGAAAGACCAGCAATGGCTCTTTTCAAATGGTCTTTCGGGAAGTCTTCATTATGGCATCTAACAATTCACATGCTTTCACATTGCCAATTATTTAGAATGCCTAGTGGGTTTAATCGGAGTTCTTGGAGATGACTTGTCGAGTACTGGAAACtGTCTCATGTAAAATATCAATTTTTTGATGTCATCTTTAAACGTAAAAAAACTAATATGTGCTCGCTAGTTAAGAGTGTAACTTAATATCACA

Fln Alignment:

sp_v1.1_unigene8405___SNEDQRTEGRTNKFILGDSYRRRLFFNYERRIRMRSSPEKVFEYFASFRTPDGELFMTPADLVRAVVPVFPPPDSNIVRDGSLRGERSPGELHCPQSELFMRFDTNGDGLISFPEYIFFVTLLSIPESDILATIKMFDLDRNGXXXXXXXXXXXXFLRANNRQGSAQRDGLRIGLKVDKSVDNGGLVEYxxxxDGKDRLQLMNFAQFVKDLHKEITRLEFLHYDHQGCGSISARDFALSMVAAADVICVNRYLDRVDDLSKDPSLSNIHITREEFENFAELRKKLKPLSLAISSYGKVYGILTKSDFRRAASHVCDVDVTGNMVDIIFYIFDKNNDGNLSTDEFLAVLERREKDIAYPIESGIIHSLTSWWKCAK
Q0DZK7______________AEEEEREEKR--RFLFGDSYRRRVFFNYEKRIRTRSPPEKIFEYFASIRNPEGEVYMLPADLMRAVVPVFPPSESKIVREGSLRGERNPGELHCAPSEFFMLFDTNGDGLISFAEYIFFVTLLSIPESSFNIAFKMFDLDHSGEIDKEEFKKVMALMRSYHRQGAAHRDGLRFGLKVGQSVENGGLVEYxxxxDGNEQLRYDKFSNFLKQLHDEIVRLEFSHYDVKSSKTISVKDFALSMVASADMNHINKLLDRVDDFDDYPDLKDLRITFEEFKAFADLRRKLEPFAMAIFSYGKVNGLLTKQDLKRAATHVCEVDLTDKVVDVIFLVFDANRDGSLSADEFLRALQRRESDIRQPASSGLMGVFTCLLNCTK

SNPs (tot: 53)

Position	A %	C %	G %	T %	Probability
734		14		85	0.993533
735	14		85		0.993533
754	14		85		0.993533
756		85		14	0.993533
758			14	85	0.993533
759		85		14	0.993533
770		14	85		0.993533
778	14		85		0.993533
792		14		85	0.993533
793			14	85	0.993533
805	85			14	0.993533
812		14		85	0.993533
819		85	14		0.993533
825	14		71		0.984337
846		14		85	0.993533
881	20	80			0.995493
904	25		75		0.996473
1401		22	77		0.999999
1466	25		75		1.0
1477			28	71	1.0
1500		33		66	1.0
1531		80		20	0.999996
1550		88		11	0.923564
1561		11		88	0.923564
1677		57		42	1.0
1745		14		85	0.993533
1795		71		28	1.0
1849		80		20	0.995493
1932		20		80	0.995493
1979			14	14	0.984337
1981		83	16		0.994512
1985	83		16		0.994512
1986	16			83	0.994512
1988	16		83		0.994512
1990		16		83	0.994512
1991	83	16			0.994512
1992	16	83			0.994512
1993	16	83			0.994512
1995	83		16		0.994512
2002			16	83	0.994512
2124	50	50			1.0
2127	83			16	0.994512
2128	83		16		0.994512
2130		83	16		0.994512
2132			16	83	0.994512
2138	20			80	0.995493
2139	20	80			0.995493
2141	80		20		0.995493
2143		80	20		0.995493
2144	40	60			1.0
2145		80		20	0.995493
2146	20	60	20		0.99255
2148	20	20		60	0.99255

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain