SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene8268

Length: 1715 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene8268

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Subtilisin-like protein n=1 Tax=Picea abies RepID=Q40764_PICAB	-	-	77%
FL-Next	tr=Subtilisin-like protein; Picea abies (Norway spruce) (Picea excelsa).	-	-	94%
Blast2go	subtilisin-like protein	-	-	65%

Source	GOs	Term	Type	e value	Identity
Blast2go	catalytic activity	GO:0003824	Molecular Function	0.0	65%
Blast2go	apoplast	GO:0048046	Cellular Component	0.0	65%

Source	InterPros	Term	Type	Identity
Blast2go	Peptidase S8/S53, subtilisin/kexin/sedolisin	IPR000209	-	65%
Blast2go	Peptidase S8, subtilisin-related	IPR015500	-	65%
Blast2go	Peptidase S8, subtilisin, Ser-active site	IPR023828	-	65%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: Q40764

Fln msg: Overlapping hits, possible frame ERROR between 125 and 122, Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 508 - 779

Fln protein: L*RCS*DGVDIVSISIGVRSSNQADFMNDAIAIGAFHATxxxILVVSSAGNEGPDSQTVVNAAPWIITVGATSIDRDFQSNVVLGNGKIIQGKGITFSNLSQSAVHPLVYGGSIPAKSCDRPFDPVAASNCLLDCLDANKTKGKIVVCIANETATSRGYMMAEIEDFTGAVGVIVVDNLQLYEAFEYGTFAGTAVSKSSATEIFSYINSTRNPVATITPSTVVANEKPAPVVASFSSRGPGALTQNILKPDISAPGLNIIAAWDPPNTFIDTVLSETMSTAFYMMSGTSVAVPHVSGAAAFLKSMNPTWSSSVIRSALMTTAIVRNNMGNLLTNASDIDGTPFDFGAGVVNPIGALQPGLVYETSTDDYFHFLCNYGLDAENIKLIAANKTYRCPSGAKVDMISNMNYPSIAISKLNVVNGSTIVSRSVTNISPDLAPTYKVTIGAPPGLSVKVSPEILQFSQTSKKLSFDVVFTPTKVATKGYVFGSLVWSDGKHNVRTPFAVNLV*

Protein Length: 509

Fln nts: GCCTTTGACGATGCAGTTAAGATGGAGTGGATATTGTATCCATCTCAATTGGAGTTCGGTCCAGTAATCAGGCCGATTTTATGAACGATGCAATTGCCATAGGAGCATTTCATGCTACTxxxxxxxxxATTCTGGTTGTATCCTCTGCAGGAAACGAGGGACCGGATTCCCAAACAGTGGTTAATGCTGCACCATGGATTATTACAGTTGGAGCGACCTCAATCGACCGGGATTTCCAGTCAAACGTTGTGTTAGGCAACGGTAAGATTATTCAGGGTAAGGGGATCACATTTTCGAATCTCAGCCAATCGGCCGTGCACCCGCTTGTGTACGGAGGAAGCATACCTGCCAAGTCCTGTGACCGACCCTTTGACCCAGTAGCGGCAAGCAATTGTCTTCTAGATTGCTTAGATGCAAACAAAACGAAAGGAAAGATTGTAGTCTGTATTGCAAATGAGACAGCTACATCGAGGGGATACATGATGGCAGAAATCGAGGACTTCACTGGAGCAGTAGGCGTGATTGTCGTGGACAACTTACAGCTATATGAAGCATTTGAGTACGGTACCTTTGCAGGGACTGCAGTAAGTAAATCGAGTGCAACAGAGATATTTTCTTACATAAACTCAACCAGAAATCCTGTTGCAACAATTACTCCATCAACGGTGGTTGCAAATGAGAAGCCGGCTCCTGTTGTTGCATCTTTTTCATCAAGAGGTCCCGGAGCGCTGACCCAGAACATTCTCAAGCCAGACATCAGCGCACCAGGTCTGAACATCATTGCTGCATGGGATCCTCCCAATACATTTATAGACACAGTTCTTTCAGAGACGATGTCTACCGCATTTTACATGATGTCAGGAACATCCGTAGCAGTTCCTCATGTTAGCGGAGCTGCTGCATTTTTAAAATCTATGAATCCTACTTGGAGCTCCTCTGTTATCAGATCAGCCCTGATGACAACAGCCATTGTAAGGAACAATATGGGAAATTTGTTGACTAATGCATCAGATATCGATGGGACGCCATTTGATTTTGGAGCGGGAGTAGTAAATCCAATTGGAGCTCTGCAACCAGGACTTGTATATGAAACATCAACCGATGATTACTTCCACTTTCTATGTAATTACGGTCTAGACGCTGAGAATATCAAGCTCATCGCTGCAAATAAAACCTATAGGTGTCCATCCGGTGCAAAGGTGGATATGATCAGTAACATGAACTATCCAAGTATAGCCATTTCCAAGTTAAATGTTGTGAATGGGAGCACAATTGTAAGCAGAAGCGTCACAAACATCAGTCCTGATCTCGCACCCACTTACAAGGTTACCATCGGTGCACCTCCGGGTCTGAGTGTGAAGGTCTCTCCAGAAATTCTTCAATTCTCTCAAACTTCCAAGAAGCTATCTTTCGACGTAGTTTTTACACCAACCAAGGTTGCCACCAAAGGATATGTTTTCGGTTCGCTCGTTTGGAGCGATGGAAAGCATAACGTTCGTACTCCTTTTGCAGTGAATCTGGTGTGACCAACCTTATAGAGGGTCAAGTAATAAAACAAACGAAACCAGCAAGGCTACTTATTGCTTGCATAGACCCTAAAGTGATAAACAAGCGAAACTTGCTTTTTGTTAGATATAATTATCTCTCGCAATTTCTTGCACAGTCATCAATATAATGGTAAAGAGGAAAAAATGAGTTTCTAAATTTGTTTTTTTG

Fln Alignment:

sp_v1.1_unigene8268___DGVDIVSISIGVRSSNQADFMNDAIAIGAFHATQxGILVVSSAGNEGPDSQTVVNAAPWIITVGATSIDRDFQSNVVLGNGKIIQGKGITFSNLSQSAVHPLVYGGSIPAKSCDRPFDPVAASNCLLDCLDANKTKGKIVVCIANETATSRGYMMAEIEDFTGAVGVIVVDNLQLYEAFEYGTFAGTAVSKSSATEIFSYINSTRNPVATITPSTVVANEKPAPVVASFSSRGPGALTQNILKPDISAPGLNIIAAWDPPN-TFIDTVLSETMSTAFYMMSGTSVAVPHVSGAAAFLKSMNPTWSSSVIRSALMTTAIVRNNMGNLLTNASDIDGTPFDFGAGVVNPIGALQPGLVYETSTDDYFHFLCNYGLDAENIKLIAANKTYRCPSGAKVDMISNMNYPSIAISKLNVVNGSTIVSRSVTNISPDLAPTYKVTIGAPPGLSVKVSPEILQFSQTSKKLSFDVVFTPTKVATKGYVFGSLVWSDGKHNVRTPFAVNLV
Q40764______________DGVDIVSISIGVRSSNQADFVKDAIAIGAFHATQxGILVVSSAGNEGPDSQTVVNAAPWIFTVGATSIDREFLSNVVLGNGKIIKGKGITMSNLSHSAVHPLVYAGSIPDKSSY----PVAASNCLLDSLDASKAKGNVVVCIANDTAASRYIMKLAVQD-AGGIGMVVVEDIQIFEAFDYGTFPATAVSKTSATEIFSYIKSNRNPVATITLTEVVTNYIPAPVIASFSSRGPGGLTQNILKPDISAPGVNIIAAWNPPNQSDEDTVVSEMTPSTFNMMSGTSVAVPHVTGAAAFVKSINPTWSSSAIRSALMTTAIVRNNMGKLLTNESDIPGTPFDFGAGVVNPIGALQPGLVYETSIDDYFHFLCNYGLDSENIKIIAANESYKCPSGVNADLISNMNYPSIAISKLGIKNGSTTISRSVTNFVPEQAPTYKVTIDAPPGLNVKVSPEILHFSKTSKKLSFNVVFTPTNVATKGYAFGTLVWSDGKHNVRSPFAVNMV

SNPs (tot: 31)

Position	A %	C %	G %	T %	Probability
460		75		25	0.996473
461	75		25		0.996473
463		75	25		0.996473
556		33		66	0.997452
722		66		33	0.997452
806	50		50		1.0
825		50		50	1.0
849	50			50	1.0
860	50			50	1.0
933		50		50	1.0
939	16		83		0.994512
940		50		50	1.0
968		75	25		0.996473
986	50		50		1.0
990	75		25		0.996473
992	25			75	0.996473
995	25		50	25	0.995533
996	50			50	1.0
998	25		50	25	0.995533
1006	25	75			0.996473
1013		50		50	1.0
1018	66	33			0.997452
1061		50		50	1.0
1099		33		66	1.0
1166	33		66		0.997452
1206	50	50			1.0
1415		80		20	0.995493
1487	33		66		0.997452
1518	14	85			0.993533
1535	83			16	0.994512
1622		33		66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain