SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene8257

Length: 1628 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene8257 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	D2/4-type cyclin (Fragment) n=1 Tax=Populus trichocarpa RepID=A8Y907_POPTR	-	-	46%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	52%
Blast2go	d-type cyclin	-	-	67%

Source	InterPros	Term	Type	Identity
Blast2go	Cyclin, C-terminal	IPR004367	-	67%
Blast2go	Cyclin, N-terminal	IPR006671	-	67%
Blast2go	Cyclin-like	IPR013763	-	67%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9Y7

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 99 aas, putative chimeric sequence, your sequence is shorter than subject: 236 - 347

Fln protein: MAPSFDCVANAYIESCEDQEKLRQNAQILAQSGENDVDEPVSMLVQRETHYMLPEDYLQRLRNRTLDVNVRREAVGWILKVHSFYNFGPPTAYLAVNYLDRFLSRHRMPQGVKAWMIQLMAVACLSLAAKMEETQVPFPSDLQREDARFIFDARTIQRMELLILSTLQWGMRSITPFSFIDYFAYRAVQGHGHDATPPKAVMSRAIELILSTTEEIDFMEYRPSAIAAAALFFVV*

Protein Length: 237

Fln nts: CGTCGTGACCTTGGATGGCACTCATTTATCTGTGTCTGTCTGCCTGCCCTCTGGGCTTTTGGGATTCGGAGATGTGAAGAAAATGAGGTGGGAAGGGAGTAGATAACCAGAAGAAGATGAGCAAGATCTGGATTTGATACCAGCCAGCC_-_ATGGCGCCAAGCTTCGATTGTGTGGCCAATGCTTACATCGAATCCTGTGAAGACCAGGAGAAGCTCAGACAAAATGCCCAAATCTTGGCCCAATCTGGCGAGAACGATGTTGATGAACCAGTCTCCATGTTGGTACAAAGAGAGACCCATTACATGCTTCCAGAGGACTATTTGCAGAGGCTCCGGAATCGAACTCTCGACGTCAATGTTCGTCGAGAGGCCGTGGGCTGGATTCTGAAGGTGCATTCTTTCTACAATTTCGGGCCTCCAACGGCCTACCTGGCTGTCAATTACCTGGATCGATTTCTCTCGAGGCACAGAATGCCGCAAGGTGTGAAGGCTTGGATGATTCAGCTCATGGCCGTGGCCTGCTTGTCTCTCGCGGCTAAGATGGAGGAGACCCAGGTTCCATTCCCTTCGGACTTGCAGAGGGAGGACGCCAGATTCATCTTTGATGCTCGCACTATTCAAAGAATGGAGCTTCTGATCTTGAGTACATTGCAATGGGGAATGCGCTCCATCACGCCATTCTCATTTATCGATTACTTTGCTTACAGGGCTGTTCAAGGTCATGGCCATGATGCTACTCCTCCAAAAGCTGTGATGTCCAGAGCCATTGAACTCATTCTCAGTACCACAGAGGAAATAGATTTCATGGAGTATAGGCCATCGGCAATAGCTGCAGCAGCCCTGTTTTTTGTTGTCTAG___TCTGAAGAGGTGGTCGCCTCTGCTAAGCAGTCCATTACAAAAGGGCTTTGTCCTCCTCCATTACTGATGTTGATAAAGACAAAATGTTTGGATGTTACAATCTGATTCAAGAGACCATCATAGAAGGAGGCTGCTACTGGACTCCAATGTCCCTACAATCCACTGATAAAACTCCAGTGGGAGTGCTGGATGCAGCAGCCTGTTTAAGCAATACACCCACCTCAAGCTACAGCGTGAAGCCATATGCCTCTGTTACAGCAGCCAAGAGGAGAAAATTGAATGAAATCTGCAGTGCATTGTTGGTATCTCAAGCCCATCCCTGTTGACACAGTAGGAGGTCCCCAGCTTCGGTTGTCGAGATTGGAGAGTGGGATCTGGTAGGGTTTTAATGGAGCAGCAGATGCAAAACCCTATATAGATATATAATCGACTGTAAATGGGGTATAGAGTACTTGCTTAGCCTGCAAATTCCTGTCCCTTCCTCGCCTATTTGACCAACTGGTTTGGCCCAGACTGATTTTGTCTTGTTTTAGAGGAGGGTTTAACTCAGTTTGAATAATGGAGGATGATGATGACAAATTACCCATAATATCAGTGAAGAAACTCATACACTGGACAGGCCAACCTTCCAAATATGTGTTTAGAAAACCTTTCTCTTCTTTTTATGTCACACTCATCCAATCAAACAGGCCCACCTTTCCATATATCTCAGGTGGTTAGGTGAAACAATTGTTATCCTTCTTACCCAGGTTCACATCCCTGCTACCCCAT

Fln Alignment:

sp_v1.1_unigene8257___MAPSFDCVAN----------AYIESCEDQEKLRQNAQILAQSGE--------NDVDEPVSMLVQRETHYMLPEDYLQRLRNRTLDVNVRREAVGWILKVHSFYNFGPPTAYLAVNYLDRFLSRHRMPQGVKAWMIQLMAVACLSLAAKMEETQVPFPSDLQREDARFIFDARTIQRMELLILSTLQWGMRSITPFSFIDYFAYRAVQGHGHDATPPKAVMSRAIELILSTTEEIDFMEYRPS
D5A9Y7______________MAPSFDCVPNLLCAEDASVVAWDDDDDDMDENVNNESICLHSSDLEGFPDLPTEDDESISFLVEKECDHMPQDGYLQRFQSRTLDVSVRQDGLSWILKVHAYYNFGPLTAYLAINYLDRFLSSYQMPQG-KAWMLQLLSVSCLSLAAKMEETHVPLLLDLQIEDAKYVFEARTIERMELLILTTLKWRLRSITPFSFLHYFVHRA----AGDQSPPRALITRSIELIVATIRVIHLVGHRPS

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
1570		12	87		0.992554
1571	87	12			0.992554
1585	66	33			1.0
1586		33	66		1.0
1588			66	33	1.0
1606	75	25			0.996473
1607		25	75		0.996473
1608	75	25			0.996473
1609		25		75	0.996473
1611	25			25	0.995533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain