SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene8088

Length: 1627 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene8088

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Malic enzyme n=1 Tax=Picea sitchensis RepID=B8LRQ8_PICSI	-	-	94%
FL-Next	sp=Malic enzyme; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	94%
Blast2go	protein	-	-	88%

Source	ECs	Term	Type	e value	Identity
Blast2go		EC:1.1.1.4-	-	0.0	88%

Source	GOs	Term	Type	Identity
Blast2go	metabolic process	GO:0008152	Biological Process	88%
Blast2go	cobalt ion binding	GO:0050897	Molecular Function	88%
Blast2go	zinc ion binding	GO:0008270	Molecular Function	88%
Blast2go	malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity	GO:0004473	Molecular Function	88%
Blast2go	chloroplast	GO:0009507	Cellular Component	88%

Source	InterPros	Term	Type	Identity
Blast2go	Malic oxidoreductase	IPR001891	-	88%
Blast2go	Malic enzyme, N-terminal	IPR012301	-	88%
Blast2go	Malic enzyme, NAD-binding	IPR012302	-	88%
Blast2go	Malic enzyme, conserved site	IPR015884	-	88%
Blast2go	NAD(P)-binding domain	IPR016040	-	88%

Full-Lengther Next Prediction

Fln status: N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LRQ8

Fln msg: Distance to subject end: 189 aas, your sequence is shorter than subject: 449 - 581

Fln protein: MATMSCLMRVSSMNKPIKSTLISDGVIHLRRRDIVQNIPGSYRIFSRVAGFRTSGETMNGDCKPNAAATVAGGVHDVYGEDRASEEQVITPWSRTVASGYVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQVKRLMHLIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTIFRRPQGLYISLKEKGKVHDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPITIDVGTNNEMLLNDEFYIGMKQKRATGQEYDDLLDEFMYAAKQAFGEKVLIQFEDFANHNAFKLLEKYRSTHLVFNDDIQGTASVVLAGLVASLKLTGGTLADHTFLFLGAGEAGTGIAELIALEITKQGKISLNEARKRIWLVDSKGLIVKSRMESLQPFKKPWAHEHEPCKG

Protein Length: 450

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTCTCCGTTGAGCCGATGGGTTTTCCTGCCTGCCAATGGTTTACCATTGGAGAACTATCTTCCCCAAGTAAACCATTGGACATTTGAAGCGGAAGGCCTTTGGGTTGAAATCAACCCACCGTTCCATCCATGTTCATACTGTTGAATTAGAGGGACACGACTGGTACTGCGGCTCGTGTCTGGTCTACCTAACAACTCAAATATATAGAATACAACATTGACCAACTCGGGAAGGAAAGCCACAGCCAATCCC_-_ATGGCAACCATGTCATGCCTGATGCGCGTTTCGTCGATGAATAAGCCTATAAAATCCACCCTTATATCAGATGGAGTCATACATCTTCGGAGGCGTGACATAGTGCAAAACATACCGGGCAGCTATAGAATATTTAGCAGAGTTGCAGGATTTCGCACTTCAGGCGAAACCATGAATGGAGATTGCAAGCCCAATGCTGCTGCCACTGTTGCAGGAGGCGTCCATGATGTCTATGGCGAAGATAGGGCTTCAGAAGAGCAGGTTATCACTCCTTGGAGCCGAACAGTTGCCAGTGGTTATGTATTGCTGAGAGATCCTCGCTACAACAAAGGTTTGGCATTCACAGATGACGAAAGGGATTATCACTATGTACGAGGTCTTCTACCGCCTGCTCACATGTCACAAGAACTTCAAGTGAAGCGTTTAATGCATCTTATTCGTCAATACAATACACCCTTGCAGAAGTACATGGCTTTGATGGATCTTGAGGAGAGGAACGAAAGGTTATTTTACAAGCTCATGATTGATCATGTCGAAGAGTTGCTTCCTATAGTCTATACACCCACAGTGGGAGAAGCTTGCCAAAAATATGGAACTATTTTTAGGCGTCCACAGGGCCTTTACATAAGCTTAAAAGAAAAGGGCAAGGTTCATGATGTTTTGAGGAACTGGCCTGAAAAGAACATTAAGGTCATTGTTGTTACTGATGGTGAACGCATTTTGGGACTGGGGGACCTTGGATGCCAGGGTATGGGGATCCCTGTTGGAAAACTAGCACTCTACACGGCACTTGGTGGAATCCGCCCTTCTGAGTGCCTTCCAATAACAATTGACGTGGGGACTAACAATGAGATGCTGCTAAATGATGAGTTCTACATAGGAATGAAGCAGAAGCGTGCTACAGGCCAGGAATATGATGATCTTCTTGATGAGTTTATGTATGCCGCCAAACAAGCTTTTGGAGAGAAAGTCCTTATCCAGTTTGAAGATTTTGCGAATCACAATGCCTTCAAATTGCTTGAAAAGTATAGATCAACACACTTGGTCTTCAATGATGATATTCAGGGAACAGCTTCAGTGGTGCTTGCAGGCCTTGTCGCATCATTAAAATTGACTGGTGGAACATTGGCTGACCACACCTTCCTATTCCTTGGTGCTGGTGAGGCAGGGACAGGTATTGCAGAGCTTATAGCATTGGAAATAACCAAACAGGGTAAAATCTCATTGAATGAAGCTCGCAAGAGGATATGGTTGGTGGACTCTAAGGGACTCATTGTGAAGTCAAGAATGGAGTCTCTTCAACCCTTCAAGAAGCCATGGGCACATGAGCATGAACCCTGCAAAGGA

Fln Alignment:

sp_v1.1_unigene8088___MNGDCKPNAAATVAGGVHDVYGEDRASEEQVITPWSRTVASGYVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQVKRLMHLIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTIFRRPQGLYISLKEKGKVHDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPITIDVGTNNEMLLNDEFYIGMKQKRATGQEYDDLLDEFMYAAKQAFGEKVLIQFEDFANHNAFKLLEKYRSTHLVFNDDIQGTASVVLAGLVASLKLTGGTLADHTFLFLGAGEAGTGIAELIALEITKQGKISLNEARKRIWLVDSKGLIVKSRMESLQPFKKPWAHEHEPCKG
B8LRQ8______________MNGDSKSTGAATLAGGVHDVYGEDRASEEQIVTPWSRTVASGNVLLRDPRYNKGLAFTDDERDYHYVRGLLPPAHMSQELQEKRLMHIIRQYNTPLQKYMALMDLEERNERLFYKLMIDHVEELLPIVYTPTVGEACQKYGTIFRRPQGLYISLKEKGKVYDVLRNWPEKNIKVIVVTDGERILGLGDLGCQGMGIPVGKLALYTALGGIRPSECLPITIDVGTNNEKLLNDEFYIGLRQKRATGQEYDDLLNEFMYAAKKAYGEKVLIQFEDFANHNAFNLLAKYRSTHLVFNDDIQGTASVVLSGLVASLKLTGGTLADHTFLFLGAGEAGTGIAELIALEMTKQGKISLNEARKRIWLVDSKGLIVKSRMESLQHFKKPWAHEHEPCKG

SNPs (tot: 30)

Position	A %	C %	G %	T %	Probability
175		25		75	0.996473
260	20	80			0.995493
262		80	20		0.995493
321			14	85	0.993533
344		75	25		0.996473
361	14		85		0.993533
362	14			71	0.984337
364			14	71	0.984337
365		71	28		1.0
408	85			14	0.993533
409			85	14	0.993533
410	12	87			0.992554
411		12		87	0.992554
412	88	11			0.923564
413			11	88	0.923564
414	77			22	0.999999
415		11	88		0.923564
417	88		11		0.923564
419	77	11	11		0.968132
420	11			88	0.923564
421		11		88	0.923564
422	11			88	0.923564
423	77		22		0.999999
426	88	11			0.923564
447		12		87	0.992554
472	12		87		0.992554
802	88	11			0.923564
871	76	7			0.999983
1177	83		16		0.999991
1529			42	57	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain