SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene7965

Length: 1208 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene7965 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	LEW1; LEW1 (LEAF WILTING 1); dehydrodolichyl diphosphate synthase; K11778 cis-prenyltransferase, dehydrodolichyl diphosphate synthase [EC:2.5.1.-]	-	-	7.0e-20	36%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	92%
Blast2go	unknown [Picea sitchensis]	-	-	0.0	59%

Source	ECs	Term	Type	e value	Identity
AutoFact	Transferases, Transferring alkyl or aryl groups, other than methyl groups, Transferring alkyl or aryl groups, other than methyl groups.	EC:2.5.1.-	-	7.0e-20	36%

Source	KEGGs	Term	e value	Identity
AutoFact	Ubiquinone and other terpenoid-quinone biosynthesis	00130	7.0e-20	36%
AutoFact	Cysteine and methionine metabolism	00270	7.0e-20	36%
AutoFact	Tryptophan metabolism	00380	7.0e-20	36%
AutoFact	Porphyrin and chlorophyll metabolism	00860	7.0e-20	36%
AutoFact	Terpenoid backbone biosynthesis	00900	7.0e-20	36%
AutoFact	Carotenoid biosynthesis	00906	7.0e-20	36%
AutoFact	Biosynthesis of terpenoids and steroids	01062	7.0e-20	36%
AutoFact	Metabolic pathways	01100	7.0e-20	36%
AutoFact	Biosynthesis of secondary metabolites	01110	7.0e-20	36%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LK53

Fln msg: your sequence is shorter than subject: 255 - 279

Fln protein: RRRDIWNILALFCWHTLHLIFIVIAYIATIIEKSRSLLFPKESLKKSRASQRKKPRIVGVVIESEEVETEMSKVCKLLMWLADIGVQHVSLYDMEGLLKQSKRSLEKTLSNLNTHIQLISTWEEHEKWMSESQITEQPIMTVELLSICDGKEGIAKAARYLCANTLQKINSDGQHIDLKLTEADINRGLEAAGCAGPEPDLLLVFGFTRCLLGFPAWRLPFTEIIYMGMFNSLTLDSLLNAVDEFSTKNQRYGK*

Protein Length: 256

Fln nts: AGGAGGAGAGATATATGGAATATTTTGGCCTTGTTCTGCTGGCATACTCTTCACTTGATATTTATTGTCATTGCATATATTGCAACTATTATTGAAAAATCAAGGTCTCTTTTGTTTCCCAAGGAATCTTTGAAAAAATCTAGGGCTTCACAAAGAAAGAAGCCTCGAATTGTGGGCGTGGTGATAGAAAGTGAAGAAGTTGAAACTGAAATGTCCAAAGTTTGTAAGCTTCTTATGTGGCTAGCTGATATTGGTGTGCAACATGTGTCTCTTTATGATATGGAAGGGCTATTGAAGCAATCTAAGAGGAGTCTTGAGAAGACCTTGAGTAATCTGAACACACACATACAATTAATCAGCACATGGGAGGAACATGAAAAATGGATGTCCGAATCTCAAATTACAGAACAGCCAATAATGACGGTAGAACTCCTTTCTATCTGTGATGGAAAAGAGGGTATTGCAAAGGCTGCCAGATATCTCTGTGCAAATACTCTGCAGAAAATCAATTCAGATGGACAGCACATAGATTTAAAGCTGACAGAGGCTGATATCAATAGAGGATTGGAAGCAGCTGGATGTGCAGGGCCAGAACCAGATCTTCTCTTAGTTTTTGGCTTCACTCGGTGCCTGTTGGGGTTCCCTGCATGGAGGCTTCCATTCACAGAGATCATCTATATGGGAATGTTCAATTCCCTGACATTGGACTCTTTACTGAATGCAGTGGACGAGTTCTCAACAAAGAATCAGAGATATGGAAAATGA___TTACCAGATTTGTATAATATTGTCAAGATGACCATGAATGTTTTCTATGAGCTTGGCAATATGATACTAATAATCCATAAATAAATTCCTGACTAACAGAAACAGGAAGATGTATTACAAAGATGTTTTTAATTTAGGGCCTCCATCTTGACACCCACCTTCAGATTCTCGCTTATTTTGCTATCACTAGCCTTCGTAAGCTTGGCTGAATAGATTGACAACATTCTATTTGTTGATTTGGAGATGCTTTTAGCATTCCCCTTGCATACTTTTATGATTTATCACCATACATTTCTCAGAGCCATTGGCCATCTTCAATCACTTGTGGACGTTAAAATTGAAGTTGAGATTATGGTACTACTTGGCACTATGCTACATTCTTAATTATTAAATTTAAAATTGGGATTATAATATTACATGGCATTCGCAGTGAGTGACAGGCC

Fln Alignment:

sp_v1.1_unigene7965___RDIWNILALFCWHTLHLXXXXXXXXXXXIEKSRSLLFPKESLKKSRASQRKKPRIXXXXXXXXXXXXXXXXVCKLLMWLADIGVQHVSLYDMEGLLKQSKRSLEKTLSNLNTHIQLISTWEEHEKWMSESQITEQPIMTVELLSICDGKEGIAKAARYLCANTLQKINSDGQHIDLKLTEADINRGLEAAGCAGPEPDLLLVFGFTRCLLGFPAWRLPFTEIIYMGMFNSLTLDSLLNAVDEFSTKNQRYGK
B8LK53______________REIWNILALFCWHALHLTFIVIAYVATIINKSWSLLFAKESLEKSRASQRKKPQIVGVVIESEEAETEMSKVCKLLMWLADVGVQHVSLYDMEGLLKQSKRSLEKTLGNLNTQIQLVSTWEEHEKWTSESQSTEQPIMTVELLSICDGKEGIAKAARYLCANTLQKINSEGQHIDLKLTEADINRGLEAAGCAGPEPDLLLVFGFTRCLLGFPAWRLPFTEIIYMGMLKSLTLDSLLNAVDEFSTKNQRYGK

SNPs (tot: 13)

Position	A %	C %	G %	T %	Probability
223		10		90	0.90298
228	80	20			0.999996
275	90		10		0.90298
280		90	10		0.90298
286		11	88		0.923564
287		11		88	0.923564
288	11		77		0.968132
289	11		88		0.923564
296	88	11			0.923564
299		11		88	0.923564
485		90		9	0.999872
1051		7		92	0.999664
1055	4	8		83	0.998844

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain