SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene6588

Length: 1757 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene6588 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	3-hydroxy-3-methylglutaryl coenzyme A reductase n=1 Tax=Taxus x media RepID=Q6WP38_9CONI	-	-	88%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	87%
Blast2go	3-hydroxy-3-methylglutaryl-coenzyme a reductase	-	-	91%

Source	InterPros	Term	Type	Identity
Blast2go	Hydroxymethylglutaryl-CoA reductase, class I/II	IPR002202	-	91%
Blast2go	Hydroxymethylglutaryl-CoA reductase, eukaryotic/arcaheal type	IPR004554	-	91%
Blast2go	Hydroxymethylglutaryl-CoA reductase, class I/II, NAD/NADP-binding	IPR009023	-	91%
Blast2go	Hydroxymethylglutaryl-CoA reductase, class I/II, substrate-binding	IPR009029	-	91%
Blast2go	Hydroxymethylglutaryl-CoA reductase, class I/II, catalytic domain	IPR023074	-	91%
Blast2go	Hydroxymethylglutaryl-CoA reductase, class I/II, conserved site	IPR023076	-	91%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: C0PRN6

Fln msg: Overlapping hits, possible frame ERROR between 269 and 269, your sequence is shorter than subject: 437 - 580

Test code: 1

Test Code was used to find complete genes when there was not found a reliable orthologue. The best ORF (Open Reading Frame) is shown, and only ORFs > 200pb were analyzed. Your ORF will be more reliable if a stop codon was found before the start codon. A Test Code value > 0.95 means the ORF is probably coding. A Test Code value < 0.74 means the ORF is probably non-coding. Test Code values in between 0.74 and 0.95 mean it is uncertain whether the ORF is coding or not.

Fln protein: EVAVKPSPPKALLKGIALGDNEDDDIAAAVCNGTMASHSLESTLGDCKRSASVRRKALEMMTGRSLDGLPLEGFDYGSILGQCCELPVxxVQIPVGVAGPLLLDGLEYMVPMATTEGCLVASTNRGCKAIHMSGGATSVLLKDGMTRAPVVRFASAKRAADLKYYVEDPDNYEKLSVIFNSTSRFARLQGIQCGIAGRNLYMRFSCFTGDAMGMNMVSKGVQNVLDFLQIAFPDMDVISVSGNFCADKKPTALNWIEGRGKSVVCEAVITKEVVSKALKTTVPALLELNMLKNLTGSALAGAMGGFNAHASNIVSAIFIATGQDPAQNVESSHCITMMEASNGGEDLHVSVTMPCIEVGTVGGGTQLASQAACLNLLGVKGANALSPGANAQRLARIVAGAVLAGELSLMSALASGQLVKSHMKYNRSSKDIKAIS*

Protein Length: 438

Fln nts: AAGAAGTTGCTGTTAAGCCCAGTCCTCCTAAAGCCCTGCTCAAGGGAATTGCATTGGGGGATAATGAGGATGATGATATTGCAGCTGCTGTTTGCAATGGGACGATGGCTTCTCATTCTCTGGAGTCAACTCTAGGGGACTGTAAGAGATCTGCTTCGGTGAGAAGAAAGGCCTTGGAAATGATGACTGGGAGATCTCTCGATGGGTTGCCCTTGGAGGGATTTGATTATGGATCCATTCTTGGCCAATGCTGTGAACTGCCTGTTxxxxxxGTGCAAATCCCTGTGGGAGTTGCAGGGCCTCTTCTGCTTGATGGGCTCGAGTACATGGTACCCATGGCCACGACAGAGGGCTGCTTGGTTGCAAGCACCAACAGAGGCTGCAAGGCTATTCATATGTCTGGCGGTGCTACCAGTGTCCTTCTCAAAGATGGAATGACCAGGGCCCCTGTGGTTCGATTCGCCTCTGCTAAGAGAGCTGCAGATCTCAAATATTACGTTGAGGATCCCGATAACTACGAAAAATTATCTGTGATCTTCAACAGCACCAGCAGATTTGCCAGGTTGCAAGGCATACAATGTGGAATTGCAGGGAGGAATCTGTACATGAGGTTTTCTTGCTTCACTGGGGATGCCATGGGGATGAATATGGTGTCCAAGGGTGTGCAGAATGTTTTGGATTTCCTTCAAATTGCGTTTCCTGACATGGATGTTATCAGTGTTTCTGGAAATTTCTGTGCAGACAAGAAACCCACAGCCTTGAACTGGATTGAAGGGCGTGGGAAATCAGTAGTTTGTGAGGCTGTCATCACCAAGGAAGTTGTTAGCAAAGCCTTGAAGACTACCGTTCCAGCTTTGCTGGAGCTGAATATGCTCAAGAACTTAACTGGATCTGCACTAGCTGGTGCCATGGGGGGTTTCAATGCCCATGCATCCAACATTGTCTCTGCTATTTTTATTGCGACCGGCCAAGATCCTGCCCAAAATGTTGAGAGCTCTCATTGTATTACAATGATGGAGGCTTCCAATGGTGGTGAGGATCTGCATGTATCAGTCACAATGCCTTGTATTGAGGTGGGAACAGTTGGAGGAGGAACCCAGTTGGCTTCACAAGCAGCTTGCTTGAATTTGCTTGGAGTGAAAGGAGCAAATGCACTATCCCCTGGTGCAAATGCTCAGCGCTTGGCCAGAATTGTAGCAGGAGCAGTTCTGGCTGGAGAGCTGTCTCTCATGTCTGCTTTGGCTTCAGGCCAGCTGGTGAAGAGCCATATGAAGTACAATAGATCAAGCAAGGATATCAAAGCAATCTCCTGAGACCTCCTGGTCCATAGAACACAAGAAATTCAGAATAGTTGTTCTTCATGTGATTTGTTTACATAGCAATAAACTTCTTTGATCAGCTAGGATCTCCATTTGCTGAATGTTGTCCAGGGACAGATGGAAGGGGCAATCTCTGGACCTATACTTAAGGAAGAGGGTGCTAGACATGCTACTTTCTCCTCAGAGTGGAAGGAACAGCCCCATTCATGTAGACTCTATTACTCCAAACTTTGCAGTGCTCTAATGGGGAGAGTGAGGCATGGGCTGATAAAGCTGTTTTTGAAAGGTGGGAGTGAAATCATTGATGGCCTTATAGTTCATTTCTTTGGAAGGTCTCTAACTCTCTGTATTTCATAGAATGAGCAATTGCCTAAGAATGTTGATCATTGGGATATGTATGATCACTATTTTTAATACTGACAAAAGATGCTCCTCTAAG

Fln Alignment:

sp_v1.1_unigene6588___EVAVKPSPPKALLKGIALGDNEDDDIAAAVCNGTMASHSLESTLGDCKRSASVRRKALEMMTGRSLDGLPLEGFDYGSILGQCCELPVGYVQIPVGVAGPLLLDGLEYMVPMATTEGCLVASTNRGCKAIHMSGGATSVLLKDGMTRAPVVRFASAKRAADLKYYVEDPDNYEKLSVIFNSTSRFARLQGIQCGIAGRNLYMRFSCFTGDAMGMNMVSKGVQNVLDFLQIAFPDMDVISVSGNFCADKKPTALNWIEGRGKSVVCEAVITKEVVSKALKTTVPALLELNMLKNLTGSALAGAMGGFNAHASNIVSAIFIATGQDPAQNVESSHCITMMEASNGGEDLHVSVTMPCIEVGTVGGGTQLASQAACLNLLGVKGANALSPGANAQRLARIVAGAVLAGELSLMSALASGQLVKSHMKYNRSSKDIKAIS
C0PRN6______________EVAIKPSPPQVQLKGISLGDNEDDDIATAVCNGTVASYSLEATLGDCTRSARVRRKALEMMTGRSLDGLPLEGFDYGSILGQCCELPVGYVQIPVGVAGPLVLDGLEYMVPMSTTEGCLVASTNRGCKAIHMSGGATSVLLRDGMTRAPVVRFSTAKRAADLKYYIEDPNNSENLSVIFNRTSRFARLQGLQCGIAGRNLYIRFSCFTGDAMGMNMVSKGVQNVLDFLQNEFPDMDVISVSGNFCADKKPAAVNWIEGRGKSVVCEAVITEAVVRKVLKTDVPALLELNMLKNLTGSALAGAMGGFNAHASNIVSAIFIATGQDPAQNVESSHCITMMEASNGGKDLHISVTMPCIEVGTIGGGTQLASQAACLNLLGVKGANVQSPGANSQRLARIVAGAVLAGELSLMSALAAGQLVKSHMKYNRSSKDIKAIT

SNPs (tot: 12)

Position	A %	C %	G %	T %	Probability
230	83		16		0.994512
278	83	16			0.994512
279		16	83		0.994512
282		83		16	0.994512
349			80	20	0.995493
355		80	20		0.995493
356	20	80			0.995493
357		20		80	0.995493
361	20			80	0.995493
363	80		20		0.995493
368	80	20			0.995493
371	80	20			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain