SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene6479

Length: 2511 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene6479
TGTCGTGACCTTGGCTGTCACTCAATTGTGGTTAGTTGGACCTGGTCAGGGGCAGGACACCCATAAATCGTCATAATTGGTCTTGTCATGGGACCCAAAGGACGAGTAGATAAATTGACAGTCATTGAGTCCTGCACTTATATTTACTCTGTAATGCCTCTGCCGACTAGACCCAAGAAGTGCAGGCCTCTGAACAGCGAGGAGCTGTGACGTGGATAATTCCTGACCGCTAGATTGACTCTGCTGGAAGGGAGAAGACAGAAGGAATAAGAAGAAAAGGAGGATGTGAAATGGATAATAGTTTGTTTTGACTGCCCTGGCCTGCAAATTTCTTGCCCCATTGAGCATCATTGGTTAAGTTAAGTTACAGTTTGGTTTGAGGTGTAGATAGTGAAACGGGCGTAGTAAGATGTTGCGTGGGCTCTTATGATTGGACTTGTATTTTAACCCCGTGCCCTAAGAATCTACGGAGACAGCACATCTGCGTAAGGGCAAAATCATTTGAGCCAGAATGTCTCAGCCTCTGTCTGCATTTGGTTAGAGACGGGGAAAGGGTTGGGAGTAGCGCTAATAGCCAGCTTAGTAGCTAGCCATATCTAGTGGACATCATCATAAGTTGCAGAGCCTTGGTCTTAAATCTTGAAGGGATTTGCTTGCTTGCTGCCGAATTTGGAGGCAACGAAATGGGGTTGATTTCAGGGATGGTGATCGGGATGCTGCTGGGAATCGCTCTCATGGCAGCTATGAAGAAAGCAATGATGTACCGCAGCATCAAGCGTATTAATAAGGCTGTAGATATTGCAGTTCTTGGGGACCTTGACAGAGATGACTTGAGAAAGCTTTGCGCTGACAATTATCCAGAATGGATATCATTTCCATTGTTTGAGCAGGTGAAGTGGCTTAACAAACAATTGAGCAAAATGTGGCCTTTTGTTGCAGCAGCAGCATCGATGGTCATTAAAGAATCTGTGGAGCCACTTTTGGAACAGTATCGACCTACAGGAATTACTTCTTTAAAATTCAGCAAACTTTTTCTTGGGAATGTAGCTCCAAAAATTGAAGGTATACGAATCCAGAGCTTAAAAAAAGGTCAAATTGTAATGGATTTGGACTTACGTTGGGGTGGCGATCCCAGTATAATCCTTTCAGTTCAAACACTTGGTCCTTCTTTGCCTATACAGTTAAAGGATCTCAAGGTTTTCACTGTAGTGCGTGTGATCTTTCAACTTGCTGAAGATATCCCTTGCATTTCTGCTGTTGTGGTTGCACTTCTTTCTGAGCCTAAACCTAAAATTGATTATACGTTAAAAGCAATCGGAGGGAGCTTAACAGCTATGCCTGGACTTTCAGATATGCTTGAAGATACAGTGGATTCTATTGTGTCAGATACCCTTCAGTGGCCTCACCGAATTGTTGTTCCAATTGGAGGAGTACCTGTCGATACAAGTGAACTTGAGCTTAGACCACAAGGCCGACTTAAAGTTACAGTAGTAAGAGCAAATTCGTTGAAAAACATGGAGATGATTGGAAAATCTGATCCATATGTTGTTTTATATGTTCGGGTTATCTTTAAAGTGAAAACGAAAGTGGTTGACAACAATCTCAATCCTGAGTGGAATGAAACTTTTGACCTAGATGTTGAGGACAAGGAGACTCAAGCACTTGTATTAGAGGTGTTTGATAAAGATGAAGTTGGTCAAGATAAGAAACTTGGTGTCGTAAAATATCTATTGGCTGACTTGGAACCAGAAAATTTGAAGGATCTTACGCTGCAACTCTTGCCATCTCTTGACACACTTAAAGTCAAAGACAAAAAGGATAGGGGATCTATTACTCTCAAGGTCCATTATCATGAATATAGCAAAGAGGAGCAGTTGCTAGCATTCGAAGAGGAACAGAAACTGTTGGAGGCAAGGAAGACACTTAAAGAGGCAGGTATTATTGGCAGCACAGTAGATGCTCTCGATGGAGCTGTAGGAGGAGCAGTATCACTAGTTGGATCTGGTGTGGGACTAGTGGGTTCTGGTCTTGGAGCTGTTGGAAGTGGAATAGGGAAGGCTAGCCGTTTCATGGGTAAAAATATTTCGAACCAATTTGGTCATTCTTCAAAGAAGAACATGAAGCCTATACTCGAAAATGGCAGTGCAAAGTAGTTCTTCCGTTATTTTTGACTCATTCTCTTCTGTAGAGATTGTCTTGCTATAAAGAGTGATATAGAGTAGAAATTTCTATGCCATTCTATCCAACGGTCATTCCCTCTGAACTACCAGTTGTATATCAGTACAGGCTACTTTTCAAAGTTGCATGATCCTTACCAGCAATTGGACCAGTGTATATCCTATATCTTGAGAGGACTGCTGTAAGATTTGTCTCTGTAATATTTAGCAATTTCTTATATTCTGCTTCTGCTCACTTTTTGAACAATGTTCTTTCCTTTAAACAATTGCATTTACTATGTAAATCATTATTCATTCTTCAATAAAATGCCTTTATAATAATTAGGAAGGTTTT

Ace file of the UniGene sp_v1.1_unigene6479

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	NTMC2T4.1; integral membrane single C2 domain protein	-	-	59%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	36%
Blast2go	integral membrane single c2 domain protein	-	-	82%

Source	InterPros	Term	Type	Identity
Blast2go	C2 calcium-dependent membrane targeting	IPR000008	-	82%
Blast2go	C2 calcium/lipid-binding domain, CaLB	IPR008973	-	82%
Blast2go	C2 membrane targeting protein	IPR018029	-	82%
Blast2go	C2 domain	IPR020477	-	82%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LRA0

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 49 aas, putative chimeric sequence, your sequence is shorter than subject: 490 - 536

Fln protein: MGLISGMVIGMLLGIALMAAMKKAMMYRSIKRINKAVDIAVLGDLDRDDLRKLCADNYPEWISFPLFEQVKWLNKQLSKMWPFVAAAASMVIKESVEPLLEQYRPTGITSLKFSKLFLGNVAPKIEGIRIQSLKKGQIVMDLDLRWGGDPSIILSVQTLGPSLPIQLKDLKVFTVVRVIFQLAEDIPCISAVVVALLSEPKPKIDYTLKAIGGSLTAMPGLSDMLEDTVDSIVSDTLQWPHRIVVPIGGVPVDTSELELRPQGRLKVTVVRANSLKNMEMIGKSDPYVVLYVRVIFKVKTKVVDNNLNPEWNETFDLDVEDKETQALVLEVFDKDEVGQDKKLGVVKYLLADLEPENLKDLTLQLLPSLDTLKVKDKKDRGSITLKVHYHEYSKEEQLLAFEEEQKLLEARKTLKEAGIIGSTVDALDGAVGGAVSLVGSGVGLVGSGLGAVGSGIGKASRFMGKNISNQFGHSSKKNMKPILENGSAK*

Protein Length: 491

Fln nts: TGTCGTGACCTTGGCTGTCACTCAATTGTGGTTAGTTGGACCTGGTCAGGGGCAGGACACCCATAAATCGTCATAATTGGTCTTGTCATGGGACCCAAAGGACGAGTAGATAAATTGACAGTCATTGAGTCCTGCACTTATATTTACTCTGTAATGCCTCTGCCGACTAGACCCAAGAAGTGCAGGCCTCTGAACAGCGAGGAGCTGTGACGTGGATAATTCCTGACCGCTAGATTGACTCTGCTGGAAGGGAGAAGACAGAAGGAATAAGAAGAAAAGGAGGATGTGAAATGGATAATAGTTTGTTTTGACTGCCCTGGCCTGCAAATTTCTTGCCCCATTGAGCATCATTGGTTAAGTTAAGTTACAGTTTGGTTTGAGGTGTAGATAGTGAAACGGGCGTAGTAAGATGTTGCGTGGGCTCTTATGATTGGACTTGTATTTTAACCCCGTGCCCTAAGAATCTACGGAGACAGCACATCTGCGTAAGGGCAAAATCATTTGAGCCAGAATGTCTCAGCCTCTGTCTGCATTTGGTTAGAGACGGGGAAAGGGTTGGGAGTAGCGCTAATAGCCAGCTTAGTAGCTAGCCATATCTAGTGGACATCATCATAAGTTGCAGAGCCTTGGTCTTAAATCTTGAAGGGATTTGCTTGCTTGCTGCCGAATTTGGAGGCAACGAA_-_ATGGGGTTGATTTCAGGGATGGTGATCGGGATGCTGCTGGGAATCGCTCTCATGGCAGCTATGAAGAAAGCAATGATGTACCGCAGCATCAAGCGTATTAATAAGGCTGTAGATATTGCAGTTCTTGGGGACCTTGACAGAGATGACTTGAGAAAGCTTTGCGCTGACAATTATCCAGAATGGATATCATTTCCATTGTTTGAGCAGGTGAAGTGGCTTAACAAACAATTGAGCAAAATGTGGCCTTTTGTTGCAGCAGCAGCATCGATGGTCATTAAAGAATCTGTGGAGCCACTTTTGGAACAGTATCGACCTACAGGAATTACTTCTTTAAAATTCAGCAAACTTTTTCTTGGGAATGTAGCTCCAAAAATTGAAGGTATACGAATCCAGAGCTTAAAAAAAGGTCAAATTGTAATGGATTTGGACTTACGTTGGGGTGGCGATCCCAGTATAATCCTTTCAGTTCAAACACTTGGTCCTTCTTTGCCTATACAGTTAAAGGATCTCAAGGTTTTCACTGTAGTGCGTGTGATCTTTCAACTTGCTGAAGATATCCCTTGCATTTCTGCTGTTGTGGTTGCACTTCTTTCTGAGCCTAAACCTAAAATTGATTATACGTTAAAAGCAATCGGAGGGAGCTTAACAGCTATGCCTGGACTTTCAGATATGCTTGAAGATACAGTGGATTCTATTGTGTCAGATACCCTTCAGTGGCCTCACCGAATTGTTGTTCCAATTGGAGGAGTACCTGTCGATACAAGTGAACTTGAGCTTAGACCACAAGGCCGACTTAAAGTTACAGTAGTAAGAGCAAATTCGTTGAAAAACATGGAGATGATTGGAAAATCTGATCCATATGTTGTTTTATATGTTCGGGTTATCTTTAAAGTGAAAACGAAAGTGGTTGACAACAATCTCAATCCTGAGTGGAATGAAACTTTTGACCTAGATGTTGAGGACAAGGAGACTCAAGCACTTGTATTAGAGGTGTTTGATAAAGATGAAGTTGGTCAAGATAAGAAACTTGGTGTCGTAAAATATCTATTGGCTGACTTGGAACCAGAAAATTTGAAGGATCTTACGCTGCAACTCTTGCCATCTCTTGACACACTTAAAGTCAAAGACAAAAAGGATAGGGGATCTATTACTCTCAAGGTCCATTATCATGAATATAGCAAAGAGGAGCAGTTGCTAGCATTCGAAGAGGAACAGAAACTGTTGGAGGCAAGGAAGACACTTAAAGAGGCAGGTATTATTGGCAGCACAGTAGATGCTCTCGATGGAGCTGTAGGAGGAGCAGTATCACTAGTTGGATCTGGTGTGGGACTAGTGGGTTCTGGTCTTGGAGCTGTTGGAAGTGGAATAGGGAAGGCTAGCCGTTTCATGGGTAAAAATATTTCGAACCAATTTGGTCATTCTTCAAAGAAGAACATGAAGCCTATACTCGAAAATGGCAGTGCAAAGTAG___TTCTTCCGTTATTTTTGACTCATTCTCTTCTGTAGAGATTGTCTTGCTATAAAGAGTGATATAGAGTAGAAATTTCTATGCCATTCTATCCAACGGTCATTCCCTCTGAACTACCAGTTGTATATCAGTACAGGCTACTTTTCAAAGTTGCATGATCCTTACCAGCAATTGGACCAGTGTATATCCTATATCTTGAGAGGACTGCTGTAAGATTTGTCTCTGTAATATTTAGCAATTTCTTATATTCTGCTTCTGCTCACTTTTTGAACAATGTTCTTTCCTTTAAACAATTGCATTTACTATGTAAATCATTATTCATTCTTCAATAAAATGCCTTTATAATAATTAGGAAGGTTTT

Fln Alignment:

sp_v1.1_unigene6479___PEWISFPLFEQVKWLNKQLSKMWPFVAAAASMVIKESVEPLLEQY-RPTGITSLKFSKLFLGNVAPKIEGIRIQSLKKGQIVMDLDLRWGGDPSIILSVQTLGPSLPIQLKDLKVFTVVRVIFQ-LAEDIPCISAVVVALLSEPKPKIDYTLKAIGGSLTAMPGLSDMLEDTVDSIVSDTLQWPHRIVVPIGGVPVDTSELELRPQGRLKVTVVRANSLKNMEMIGKSDPYVVLYV--RVIFKVKTKVVDNNLNPEWNETFDLDVEDKETQALVLEVFDKDEVGQDKKLGVVKYLLADLEPENXXXXXXXXXXXXXXXXVKDKKDRGSITLKVHYHEYSKEEQLLAFEEEQKLLEA
B8LRA0______________PLWVKNPDYDRVDWLNTFIHEMWPYLDKAICQIIRDKTKPMIEQYVGKFKIESIEIETLTLGTLPPTLQGMKVYDTQEKELIMEPVLKWAGNPNVIVAVKAFGLRATVQLVDLQVFAIPRVTLKPLVPSFPCFAKIFVSLME--KPHVDFGLKLLGGDLMAIPGLYQFAQEMIKEQVANLYLWPKTLEVPI----LDQRATH-KPVGMLHVKVVRAINLKKKDMLGKSDPYVKLKMTGEKLPSKKTTVKSSNLNPEWNEEFKFVVKDPESQALELSVYDWEKVGSHEKMGIQTYDLKELTPSETKSVTLNLLKSLDPNDPQNAKARGQITIEMTYNPFKEDENSPADDEDSVVEKA

SNPs (tot: 10)

Position	A %	C %	G %	T %	Probability
125			25	75	0.996473
130		25	75		0.996473
134		75	25		0.996473
144	20			80	0.995493
1591	83		16		0.994512
1650		14	85		0.993533
1678	87		12		0.992554
1775	12		87		0.992554
1827	90		10		0.90298
2438		16		83	0.994512

SSRs (tot: 2)

Start position	End position	Sequence	Length
650	662	TTGC TTGC TTGC T	13
936	947	GCA GCA GCA GCA	12

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain