SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene6202

Length: 1501 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene6202 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	multidrug resistance pump, putative	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	65%
Blast2go	multidrug resistance	-	-	77%

Source	GOs	Term	Type	e value	Identity
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	77%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKL5

Fln msg: Separated hits, possible frame ERROR between 639 and 653, your sequence is shorter than subject: 380 - 500

Fln protein: RRDLGCHSIFSPPILKLFGQSDEISDLAGKFAIWMLPQLFAYALNFPMQKFLQSQRKVMAMAWIAAVALVIHIFLSWLFILKLGWGLPGAAITLNLAWVIVDVGQLIYIMSGAFPGSWTGMSRLAFQDLPAFIRLSLASAVMLCLEFWYLMILIVLVGRLKNPEIAVDSISICMNLNGWEMMIALGFNAAVSVRVSNELGAGRPKAGQFAVVxxxxxxSLMIGITCMIIIFGTRNEFPVAFTNSKEVMHAVSKLATLLALTMLLNSVQPVLSGVAVGAGWQWLVAYINLGCYYIVGVPLGLILGYKLDFGAMGIWGGMIGGTVLQTLILMFITYRTNWDREASQAEDRIKLWGGSVASIKDSLRKDTGRDDREQLSSEV*

Protein Length: 381

Fln nts: CGTCGTGACCTTGGCTGTCACTCAATTTTTTCACCGCCCATATTGAAGCTTTTTGGGCAATCTGATGAGATTTCTGACTTGGCTGGGAAGTTTGCCATCTGGATGCTTCCTCAGTTATTTGCCTATGCACTAAATTTTCCAATGCAGAAGTTCCTTCAATCGCAGAGAAAGGTCATGGCCATGGCCTGGATCGCTGCAGTGGCTTTAGTTATACACATTTTCTTGAGCTGGCTCTTTATTTTGAAGCTGGGATGGGGTTTGCCAGGAGCAGCCATAACTCTCAACCTTGCATGGGTGATAGTTGATGTGGGGCAGCTGATATATATAATGTCGGGTGCCTTTCCTGGTTCATGGACTGGAATGTCACGGTTGGCATTCCAGGATTTGCCTGCCTTCATCAGGCTTTCCTTGGCTTCTGCTGTGATGCTATGCCTGGAGTTCTGGTATCTTATGATATTGATCGTTTTAGTTGGGCGCCTGAAAAATCCAGAAATTGCAGTAGACTCAATCTCCATTTGTATGAATTTGAACGGATGGGAAATGATGATAGCCCTCGGATTCAATGCTGCTGTCAGTGTGAGAGTGTCGAATGAACTTGGAGCTGGGCGCCCAAAGGCTGGACAGTTTGCAGTAGTGGTxxxxxxxxxxxxxxxGTCACTAATGATAGGAATAACATGTATGATCATTATTTTCGGCACCAGAAATGAATTTCCAGTGGCTTTTACGAATAGCAAGGAGGTTATGCATGCAGTATCCAAGCTAGCAACTTTGCTTGCACTGACAATGCTTCTCAACAGTGTGCAGCCAGTTCTATCCGGGGTGGCTGTAGGAGCAGGATGGCAATGGTTGGTGGCATATATAAATTTAGGATGCTATTACATTGTTGGGGTTCCTCTTGGTCTGATCTTGGGATACAAATTGGACTTTGGAGCAATGGGTATTTGGGGTGGCATGATTGGAGGAACAGTACTGCAGACTCTAATTCTTATGTTTATCACATATAGGACTAACTGGGACAGAGAGGCTTCACAAGCTGAAGATCGGATTAAACTTTGGGGAGGTTCAGTAGCTTCAATCAAAGACAGTTTAAGGAAGGACACTGGAAGAGATGACAGAGAGCAGTTATCTTCTGAAGTATGAAATGAGGATTGCTACACTGTTACTCTGAAGTGATGCATGTACTCTAATGCATCCACATCACCAGTTTCAGCAGATAATAGGCATTTTTAGGTGTATTTGAAGAGGACAGTGAATTCATGGTGAATTTGATTATGGTGTCTTCTTCCACAATCAAGGACAAAGAGAGTCATTTTGTCAAGCTACATTCAATTGTCTAACAAATTTTTAATTCCCCCATTCGTTGTTTTCATGGAATCACCCTGTTCTTTTATTGCAGGCTTTGACTTTTACAAATTCTGATTAATGGTATTCTATTTCCTATAACATCATGTAACTATCAGGTGATGTTGAAAAATATACAACCTTCTGCCACATTCAAGAGCA

Fln Alignment:

sp_v1.1_unigene6202___IFSPPILKLFGQSDEISDLAGKFAIWMLPQLFAYALNFPMQKFLQSQRKVMAMAWIAAVALVIHIFLSWLFILKLGWGLPGAAITLNLAWVIVDVGQLIYIMSGAFPGSWTGMSRLAFQDLPAFIRLSLASAVMLCLEFWYLMILIVLVGRLKNPEIAVDSISICMNLNGWEMMIALGFNAAVSVRVSNELGAGRPKAGQFAVVVxxxxxSLMIGITCMIIIFGTRNEFPVAFTNSKEVMHAVSKXXXXXXXXXXLNSVQPVLSGVAVGAGWQWLVAYINLGCYYIVGVPLGLILGYKLDFXXXXXXXXXXXGTVLQTLILMFITYRTNWDREASQAEDRIKLWGGS
B8LKL5______________IFATPILVLIGQQKDIADMAGKFAVWMLPQLFAYAMNFPLAKFLQAQSKVMVMSIIAGCALVVHVLLSWILIFKVGWGLPAAALVLNLSWWIIVLAQIGYVFSGACRDAWAGFSWSAFSNLGAFVRLSLASGVMLCLEAWYLYLLILLAGYLKNPTIAVDAISICMNLMGWEFMIAIGFNIAISVRVSNELGAGRPRAAKFSVIVxxxxxTFLVGVVLMAIIFITRNEFAVAFTDSKVVMSAVAQLAPLLAFTMLLNSVQPVLSGVAIGAGWQALVAYINLACYYLFGVPLGCLLGYYFDLGVEGIWAGMISGTLLQTIILCIITYRTKWNKEANQAKARITLWGGA

SNPs (tot: 9)

Position	A %	C %	G %	T %	Probability
1122		85		14	0.993533
1123		85	14		0.993533
1124	12			87	0.992554
1128	87	12			0.992554
1129	12			87	0.992554
1135		85	14		0.993533
1139		14	85		0.993533
1143		14		85	0.993533
1144			14	85	0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain