SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene5986

Length: 1102 nt

Origin of reads: Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene5986 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Serine/threonine kinase-like protein n=3 Tax=Andropogoneae RepID=B6SUE5_MAIZE	-	-	8.0e-30	34%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	41%
Blast2go	33 kda secretory expressed	-	-	5.23932e-32	50%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Gnk2-homologous domain	IPR002902	-	5.23932e-32	50%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LLG0

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 23 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 229 - 384

Fln protein: PVTAVDGKSDPSIYQLCNVNKYANGTRYESNVKTVLKYLVNAAAPRGGFATYSYGEVNGLLQCRGDVSEQDCQKCSAAASEMAFEDCPNAIGARIQLEFCFIRYEKYSFATELDTSDVYGLQNVNSNSDVKGFNHTLGILMKTLADDAPLNTKRFATGSRVVNSAVTIYGLEMCFRSVAGPDCGVCLREAIRDMNECCSAWVGAQVYLGSCTVRFETYPFYNQRAFLP*

Protein Length: 230

Fln nts: AGAACATCAGTTAATCTGTTTGTGTCTATCCATTTGTAACCGTGGAAGATGGAAGGCATCATAAGGTTGTGGCGTTGCATCACACTATTGTTGTTATTTGGTGTGCAGCACCTTAGCGTA_-_ATGACCAGTGACAGCCGTAGATGGGAAATCGGATCCGTCTATATACCAACTCTGCAACGTCAATAAATACGCCAATGGCACTCGTTACGAGAGCAACGTCAAGACAGTATTGAAGTATCTGGTGAATGCCGCGGCCCCGAGAGGCGGATTCGCAACATACTCATATGGCGAAGTCAACGGCCTTCTCCAATGCCGCGGCGACGTCTCTGAACAGGACTGTCAAAAGTGCTCCGCTGCGGCATCAGAAATGGCTTTTGAAGACTGTCCCAACGCAATTGGCGCTCGGATTCAGTTGGAATTTTGCTTCATTCGTTATGAGAAATACAGTTTTGCCACTGAACTCGATACCAGCGACGTCTATGGTCTTCAGAATGTTAACAGCAACAGTGATGTTAAAGGTTTCAATCACACTCTCGGTATTCTCATGAAGACTCTGGCAGACGACGCTCCCTTGAACACGAAAAGATTCGCCACTGGATCGCGGGTGGTGAATTCTGCTGTGACCATTTATGGGTTGGAAATGTGTTTCAGAAGTGTTGCAGGGCCCGACTGTGGTGTTTGTTTGAGAGAAGCGATTAGAGATATGAATGAGTGTTGCTCCGCATGGGTTGGAGCTCAGGTTTATCTGGGTAGTTGCACTGTACGCTTCGAGACATATCCCTTCTATAACCAGCGCGCCTTCCTGCCATGAGATTGACGTAACTCACTGAGTCGGTGCCTGCATTCAAATAATAAAGGCGGCCTCCTCTGTCTGCTTTTGCTTCTTATATTACCTGTAATATCTGCGCTTCTACCACAACGTAACCCATTCACTCCCTGTGTGCTGTCAACTAAATAAAGGTTCCCGACTGTATAGCCTGCATGTTGCTGTCAAATAAATAAAGGCTTCCTAATATATCGCCTACTGGTTATTATATTTCCTCCACCGTACGCTTCTATCGAATGGGAGTTGTGTCTAAACGCCTATTGAGATCTCAATGTA

Fln Alignment:

sp_v1.1_unigene5986___SDPSIYQLCNVNKYANGTRYESNVKTVLKYLVNAAAPRGGFATYSYGE----VNGLLQCRGD-VSEQDCQKCSAAASEMAFEDCPNAIGARIQLEFCFIRYEKYSFATELDTSDVYGLQNVNS--NSDVKGFNHTLGILMKTLADDA--PLNTKRFATGSRVVNSAVTIYGLEMCFRSVAGPDCGVCLREAIRDMNECCSAWVGAQVYLGSCTVRFETYPFYN
B8LLG0______________SELRLHACNNASSYADGSTFSSNLNRVMGDLVKNA-PQTGFNTSFYGESSNRVYGLLQCIGNNISQKDCSICSQRARDNVRQLCGNDIGGQEWRNNCFLRYENYSFFSKLDT-DGDELENANDIVTTNLSNFQTTTSNLLSYLSDQAYYPAN-KGFAQGTVEDPSVGTIYGLVQCLRDLSIKDCRSCLQSARNRIYKCCFTQQGAQSLLGSCTVRYEIYPFFN

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
147	66	33			0.997452
388	80		20		0.995493
443	16		83		0.994512
654	90			10	0.90298
659		10		90	0.90298
660	90	10			0.90298
661		20		80	0.999996
664		90	10		0.90298
814			83	16	0.999991
823	16		83		0.999991
829		16		83	0.999991
843		20		80	0.999996
844		77	22		0.999999
850	22	77			0.999999
877		28		71	1.0
923	80			20	0.995493
936			80	20	0.995493
943	75		25		0.996473
944	25		75		0.996473
946		75		25	0.996473
952		75		25	0.996473
967		25	75		0.996473
973		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain