SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene5658

Length: 2831 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene5658
CGTCGTGACCTTGGCTGTCACTCAAATTCCATGGAAAACAGGCCTTTATAGTGCACTTAATTGCACGCTGTGAGACTTATTTAATAGACCGTGTTGCAGAGGGCAGGAAGGGACGGGGGAGAAAGGGCCAAAATGGGAATTGGAATGCTCGCATTTCAGGCGGCAAAGAGATCAAAATCGCTAGCGGGGCGCTTATGGGCCGTGCAGCAGCAGCAGCGAGAGGCGTTGGGACCCAACGGCGGGGTGTGGATGCGGGCAATATCTTCGTCTCCAGCGAGGGAAACGGAAGCGAGCGCAGAGAAAAGAACGCCTGTGGCAGGAGCGAGGGTGGAGATCCCCAAGCCGGAGGAGGCTTTCGAAGTGACCGTGGATGGTTACCCAGTCAAGATTCCGAAGGGCATGACGGTGCTCCAAGCCTGCGAGTTAGCGGGGGTCGATATCCCTCGCTTCTGCTATCATTCCCGCCTCTCTATCGCCGGAAATTGCCGTATGTGTCTCGTCGAGGTCGAGAAGAGTCCCAAGCCCGTGGCATCTTGCGCCATGCCTGCGCTTCCTGGCATGAAGATAAAGACTGATACTCCGATGGCGAAAAAGGCAAGGGAAGGGGTGATGGAGTTTCTGTTGATGAATCATCCGCTGGATTGTCCAATCTGTGACCAAGGAGGGGAGTGCGATCTGCAGGATCAGTCCATGGCTTTCGGATCGGATCGCGGGCGTTTCGTCGAAACAAAAAGATCCGTGGTGGATAAGAATCTCGGTCCTCTCGTCAAGACTGTCATGACCAGGTGTATTCAGTGTACAAGGTGTGTTAGATTTGCTTCTGAAGTTGCTGGAGTACAGGACCTTGGCATGTTGGGACGTGGAAGTGGTGAAGAAATTGGCACGTATGTGGAAAGATTGATGACGAGTGAACTTTCAGGGAATGTGATAGATATCTGTCCTGTTGGAGCCCTTACATCTAAACCATTTGCATTCAAAGCAAGAAACTGGGAACTAAAGGGCACAGAGACCATTGATGTTTCTGATGCAGTTGGATCCAATATTAGAATTGATAGTCGAGGTCCAGAAGTGATGCGGGTTCTTCCTCGCCTAAATGAGGACATAAATGAAGAGTGGATATCAGACAAGACGCGGTTTTCTTATGATGGTCTTAAGCGACAAAGGCTAAATGAGCCAATGATGCGCCGACCTGATGGTCGTCTTCAACCTGTGACCTGGCACGATGCTCTTGAGGTTATTGCAGAGGTTACCCAGCAAGTAAAGCCAGAAGAAATGGTTGCAATTGCTGGGAAACTCTCTGATGTTGAATCCATAATGGCTTTGAAAGATTATATGAACAGAATGGGGTCTGAGAACATATGGTGTGAAGGGAATGGCATGAATCCTCAGGCTGATCTGCGGTCTGAATATCTCTTGAATACAAGCATTGCGGGCCTGGAGAAGGGAGATGCTTTTCTCTTTGTTGGTACAAATCCAAGAGTTGAAGCACCAATGGTGAATGCAAGGATAAGGAAAGCTGTAAGAGCAGGACATGCAAAAGTTGCTTCTGTTGGCCCTCCTACGGACTTAGCTTATGAACATCAGCATTTAGGCATTGGACCAGAAACACTTATTCAGATAGCTGAAGGCAGGCATCCGTTTTGTTCAACTCTGGCTTCTGCAAAATATCCTGTGCTAATTGTTGGGGCTGGTATTTTCGAACGGAAAGATAAAGATGCTATTATGGCTGCAGTTGATATTATTGTCAAGCATGCCAAAGTTATTAGGAATGAATGGAATGGTTTCAATGTCCTTTTACTGAATGCTGCTCAAGCTGGGGGCCTAGATCTCGGTTTTATCCCTGGTTCTGGTTCAAAGATTGGCATTGAAAATGCAAAATTTGTTTACTTGCTAGGAGCAGATGACATTCAGGTTGACAAAATCTCCAAGGATGCATTTGTAGTTTATCAAGGGCACCATGGTGATCGAAGTGTTTACAGGGCTAATATGATTTTACCAGGGGTGGCTTTTTCTGAGAAGGAAGGAACATATGAAAATGCAGAAGGTTGTACTCAGCAGACCATCCCTGCAGTTCCCACAGTTGGTGATTCTAGGGATGACTGGAAAATTCTTAGAGCTTTATCTGAAGTTTCTGGAGTAAAACTCCCATATGATACAGTAAATGCACTTCGTACTCGCATTCGAGCTGTGTCCCCAAATTTGTTTCGAGTTGATGTAGAGGGAACCTGCATCAGCATGGCTTCGAGAACTGAAGCCTGAATTCAACTCAAAAATGAGTCGAAGACCTTTTACCAATGCTATTGATAACTTCTACATGACTGATTCTATCTGTCGGGCCTCAAAAACAATGGCTCAGTGCACTGCCACATTACTCAGCCAGAAGTAATAAGCACTCCTAAGAACTTGTTTTCCAGAGCCATTAAAAATGTGCAGGATCTGCAATCTGCACACTTTGACTATTTATTCTTGTCTTTTCTGATATGTTCATGGGATCTTCTTGACGTGTAAGTGGTAGCAATGGTGACACGATGTGCTGTGCATCTACCTTTCATTGAATAATTTTGTGTGGCTTTTTTGTCTCTTTCATATCATCTTGTGCATTTATTTATTTAAATGCTGAATGACACCTAGCAGAGGAAAAACTATTTAATGGCAGAAGTGGTACTGTGCTTGAATTATTTTTTGGTTTTATTCCATGCAACTTGCATATACCCTATTTATATCTGGTTGGTAGGTGTTCGATTGTGCTGCACAATGGAGACTTTTGCTATTCAATTGTAAGTTGTAACGAGTCAGTGCCTGTTAGTATTATATTATTATTGGCACCGCTC

Ace file of the UniGene sp_v1.1_unigene5658

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	NADH-ubiquinone oxidoreductase 75 kDa subunit n=1 Tax=Picea sitchensis RepID=B8LMM8_PICSI	-	-	95%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	95%
Blast2go	protein	-	-	84%

Source	GOs	Term	Type	Identity
Blast2go	response to oxidative stress	GO:0006979	Biological Process	84%
Blast2go	metabolic process	GO:0008152	Biological Process	84%
Blast2go	oxidoreductase activity	GO:0016491	Molecular Function	84%
Blast2go	mitochondrial membrane	GO:0031966	Cellular Component	84%
Blast2go	chloroplast	GO:0009507	Cellular Component	84%
Blast2go	respiratory chain complex I	GO:0045271	Cellular Component	84%

Source	InterPros	Term	Type	Identity
Blast2go	NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site	IPR000283	-	84%
Blast2go	2Fe-2S ferredoxin-type domain	IPR001041	-	84%
Blast2go	Molybdopterin oxidoreductase	IPR006656	-	84%
Blast2go	NADH:ubiquinone oxidoreductase, subunit G	IPR010228	-	84%
Blast2go	Beta-grasp domain	IPR012675	-	84%
Blast2go	NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding	IPR019574	-	84%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LMM8

Fln msg: Separated hits, possible frame ERROR between 2214 and 2219,

Fln protein: MGIGMLAFQAAKRSKSLAGRLWAVQQQQREALGPNGGVWMRAISSSPARETEASAEKRTPVAGARVEIPKPEEAFEVTVDGYPVKIPKGMTVLQACELAGVDIPRFCYHSRLSIAGNCRMCLVEVEKSPKPVASCAMPALPGMKIKTDTPMAKKAREGVMEFLLMNHPLDCPICDQGGECDLQDQSMAFGSDRGRFVETKRSVVDKNLGPLVKTVMTRCIQCTRCVRFASEVAGVQDLGMLGRGSGEEIGTYVERLMTSELSGNVIDICPVGALTSKPFAFKARNWELKGTETIDVSDAVGSNIRIDSRGPEVMRVLPRLNEDINEEWISDKTRFSYDGLKRQRLNEPMMRRPDGRLQPVTWHDALEVIAEVTQQVKPEEMVAIAGKLSDVESIMALKDYMNRMGSENIWCEGNGMNPQADLRSEYLLNTSIAGLEKGDAFLFVGTNPRVEAPMVNARIRKAVRAGHAKVASVGPPTDLAYEHQHLGIGPETLIQIAEGRHPFCSTLASAKYPVLIVGAGIFERKDKDAIMAAVDIIVKHAKVIRNEWNGFNVLLLNAAQAGGLDLGFIPGSGSKIGIENAKFVYLLGADDIQVDKISKDAFVVYQGHHGDRSVYRANMILPGVAFSEKEGTYENAEGCTQQTIPAVPTVGDSRDDWKILRALSEVSGVKLPYDTVNALRTRIRAVSPNLFRVxxxREPASAWLRELKPEFNSKMSRRPFTNAIDNFYMTDSICRASKTMAQCTATLLSQK*

Protein Length: 753

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATTCCATGGAAAACAGGCCTTTATAGTGCACTTAATTGCACGCTGTGAGACTTATTTAATAGACCGTGTTGCAGAGGGCAGGAAGGGACGGGGGAGAAAGGGCCAAA_-_ATGGGAATTGGAATGCTCGCATTTCAGGCGGCAAAGAGATCAAAATCGCTAGCGGGGCGCTTATGGGCCGTGCAGCAGCAGCAGCGAGAGGCGTTGGGACCCAACGGCGGGGTGTGGATGCGGGCAATATCTTCGTCTCCAGCGAGGGAAACGGAAGCGAGCGCAGAGAAAAGAACGCCTGTGGCAGGAGCGAGGGTGGAGATCCCCAAGCCGGAGGAGGCTTTCGAAGTGACCGTGGATGGTTACCCAGTCAAGATTCCGAAGGGCATGACGGTGCTCCAAGCCTGCGAGTTAGCGGGGGTCGATATCCCTCGCTTCTGCTATCATTCCCGCCTCTCTATCGCCGGAAATTGCCGTATGTGTCTCGTCGAGGTCGAGAAGAGTCCCAAGCCCGTGGCATCTTGCGCCATGCCTGCGCTTCCTGGCATGAAGATAAAGACTGATACTCCGATGGCGAAAAAGGCAAGGGAAGGGGTGATGGAGTTTCTGTTGATGAATCATCCGCTGGATTGTCCAATCTGTGACCAAGGAGGGGAGTGCGATCTGCAGGATCAGTCCATGGCTTTCGGATCGGATCGCGGGCGTTTCGTCGAAACAAAAAGATCCGTGGTGGATAAGAATCTCGGTCCTCTCGTCAAGACTGTCATGACCAGGTGTATTCAGTGTACAAGGTGTGTTAGATTTGCTTCTGAAGTTGCTGGAGTACAGGACCTTGGCATGTTGGGACGTGGAAGTGGTGAAGAAATTGGCACGTATGTGGAAAGATTGATGACGAGTGAACTTTCAGGGAATGTGATAGATATCTGTCCTGTTGGAGCCCTTACATCTAAACCATTTGCATTCAAAGCAAGAAACTGGGAACTAAAGGGCACAGAGACCATTGATGTTTCTGATGCAGTTGGATCCAATATTAGAATTGATAGTCGAGGTCCAGAAGTGATGCGGGTTCTTCCTCGCCTAAATGAGGACATAAATGAAGAGTGGATATCAGACAAGACGCGGTTTTCTTATGATGGTCTTAAGCGACAAAGGCTAAATGAGCCAATGATGCGCCGACCTGATGGTCGTCTTCAACCTGTGACCTGGCACGATGCTCTTGAGGTTATTGCAGAGGTTACCCAGCAAGTAAAGCCAGAAGAAATGGTTGCAATTGCTGGGAAACTCTCTGATGTTGAATCCATAATGGCTTTGAAAGATTATATGAACAGAATGGGGTCTGAGAACATATGGTGTGAAGGGAATGGCATGAATCCTCAGGCTGATCTGCGGTCTGAATATCTCTTGAATACAAGCATTGCGGGCCTGGAGAAGGGAGATGCTTTTCTCTTTGTTGGTACAAATCCAAGAGTTGAAGCACCAATGGTGAATGCAAGGATAAGGAAAGCTGTAAGAGCAGGACATGCAAAAGTTGCTTCTGTTGGCCCTCCTACGGACTTAGCTTATGAACATCAGCATTTAGGCATTGGACCAGAAACACTTATTCAGATAGCTGAAGGCAGGCATCCGTTTTGTTCAACTCTGGCTTCTGCAAAATATCCTGTGCTAATTGTTGGGGCTGGTATTTTCGAACGGAAAGATAAAGATGCTATTATGGCTGCAGTTGATATTATTGTCAAGCATGCCAAAGTTATTAGGAATGAATGGAATGGTTTCAATGTCCTTTTACTGAATGCTGCTCAAGCTGGGGGCCTAGATCTCGGTTTTATCCCTGGTTCTGGTTCAAAGATTGGCATTGAAAATGCAAAATTTGTTTACTTGCTAGGAGCAGATGACATTCAGGTTGACAAAATCTCCAAGGATGCATTTGTAGTTTATCAAGGGCACCATGGTGATCGAAGTGTTTACAGGGCTAATATGATTTTACCAGGGGTGGCTTTTTCTGAGAAGGAAGGAACATATGAAAATGCAGAAGGTTGTACTCAGCAGACCATCCCTGCAGTTCCCACAGTTGGTGATTCTAGGGATGACTGGAAAATTCTTAGAGCTTTATCTGAAGTTTCTGGAGTAAAACTCCCATATGATACAGTAAATGCACTTCGTACTCGCATTCGAGCTGTGTCCCCAAATTTGTTTCGAGTTGAxxxxxxGAGGGAACCTGCATCAGCATGGCTTCGAGAACTGAAGCCTGAATTCAACTCAAAAATGAGTCGAAGACCTTTTACCAATGCTATTGATAACTTCTACATGACTGATTCTATCTGTCGGGCCTCAAAAACAATGGCTCAGTGCACTGCCACATTACTCAGCCAGAAGTAA___TAAGCACTCCTAAGAACTTGTTTTCCAGAGCCATTAAAAATGTGCAGGATCTGCAATCTGCACACTTTGACTATTTATTCTTGTCTTTTCTGATATGTTCATGGGATCTTCTTGACGTGTAAGTGGTAGCAATGGTGACACGATGTGCTGTGCATCTACCTTTCATTGAATAATTTTGTGTGGCTTTTTTGTCTCTTTCATATCATCTTGTGCATTTATTTATTTAAATGCTGAATGACACCTAGCAGAGGAAAAACTATTTAATGGCAGAAGTGGTACTGTGCTTGAATTATTTTTTGGTTTTATTCCATGCAACTTGCATATACCCTATTTATATCTGGTTGGTAGGTGTTCGATTGTGCTGCACAATGGAGACTTTTGCTATTCAATTGTAAGTTGTAACGAGTCAGTGCCTGTTAGTATTATATTATTATTGGCACCGCTC

Fln Alignment:

sp_v1.1_unigene5658___MGIGMLAFQAAKRSKSLAGRLWAVQQQQREALGPNGGVWMRAISSSPARETEASAEKRTPVAGARVEIPKPEEAFEVTVDGYPVKIPKGMTVLQACELAGVDIPRFCYHSRLSIAGNCRMCLVEVEKSPKPVASCAMPALPGMKIKTDTPMAKKAREGVMEFLLMNHPLDCPICDQGGECDLQDQSMAFGSDRGRFVETKRSVVDKNLGPLVKTVMTRCIQCTRCVRFASEVAGVQDLGMLGRGSGEEIGTYVERLMTSELSGNVIDICPVGALTSKPFAFKARNWELKGTETIDVSDAVGSNIRIDSRGPEVMRVLPRLNEDINEEWISDKTRFSYDGLKRQRLNEPMMRRPDGRLQPVTWHDALEVIAEVTQQVKPEEMVAIAGKLSDVESIMALKDYMNRMGSENIWCEGNGMNPQADLRSEYLLNTSIAGLEKGDAFLFVGTNPRVEAPMVNARIRKAVRAGHAKVASVGPPTDLAYEHQHLGIGPETLIQIAEGRHPFCSTLASAKYPVLIVGAGIFERKDKDAIMAAVDIIVKHAKVIRNEWNGFNVLLLNAAQAGGLDLGFIPGSGSKIGIENAKFVYLLGADDIQVDKISKDAFVVYQGHHGDRSVYRANMILPGVAFSEKEGTYENAEGCTQQTIPAVPTVGDSRDDWKILRALSEVSGVKLPYDTVNALRTRIRAVSPNLFRVDxxREPASAWLRELKPEFNSKMSRRPFTNAIDNFYMTDSICRASKTMAQCTATLLSQK
B8LMM8______________MGIGMLAFQAARRSKSLAGRLWAVQQQQQEALGPNGGAWMRAISSSPARGTEASAEKRTPVGGARVEIPKPEEAFEVTVDGHTVKIPKGLTVLQACELAGVDIPRFCYHSRLSIAGNCRMCLVEVEKSPKPVASCAMPALPGMKIKTDTPMAKKAREGVMEFLLMNHPLDCPICDQGGECDLQDQSMAFGSDRGRFIETKRSVVDKNLGPLVKTVMTRCIQCTRCVRFASEVAGVQDLGILGRGSGEEIGTYVERLMTSELSGNVIDICPVGALTSKPFAFKARNWELKGTESIDVTDAVGSNIKIDSRGPEVMRILPRLNEDINEEWISDKTRFCYDGLKRQRLNEPMIRRPDGRLQPVTWHDALEVIAEVTHQVKPEEMVAIAGKLSDVESMMALKDYMNKMGSENIWCEGDGMDPQADLRSDFLLNTSIAGLEKGDAFLFVGTNPRVEAPMVNARIRKAVRAGHAKVASVGPPMDLAYDHQHLGIGPETLIQIAEGRHPFCSTLASAKYPVIIVGAGIFERKDKDAVMAAVDIIAKHAKVIRNEWNGFNVLLLNAAQAGGLDLGLIPGSGSKNGIENAKFVYLLGADDIQVDKISKDAFVVYQGHHGDRSVYRANMILPGVAFSEKEGTYENTEGCTQQTIPAVPTVGDSRDDWKILRALSEISGVKLPYDTVNALRTRIRAVSPNLFRVDxxREPASAWIRELKPEFNSKMSPRPFNKAIDNFYMTDSICRASKTMAQCTAALLSEK

SNPs (tot: 37)

Position	A %	C %	G %	T %	Probability
12	33			66	0.997452
13		33	66		0.997452
14	33		66		0.997452
22			33	66	0.997452
23		66	33		0.997452
24	66			33	0.997452
127			66	33	0.997452
559	83		16		1.0
563	83			16	1.0
566		16		83	1.0
567	83	16			1.0
568	83			16	1.0
569	83			16	1.0
571	83		16		1.0
575	83			16	1.0
576		16		83	1.0
1431	15		84		0.999986
1468	90	10			0.90298
1469	10	90			0.90298
1554	10	90			0.90298
1605	85		14		0.993533
2051		92		7	0.999999
2052			7	92	0.999999
2053		92	7		0.999999
2054	92		7		0.999999
2106	94		3	1	0.964041
2107	96		3		0.993591
2129	1			94	0.964041
2236	87			12	0.992554
2468		8		91	0.999766
2566			73	26	1.0
2618		80		20	0.995493
2657	16		83		0.994512
2660	16		83		0.994512
2661	16			83	0.994512
2662	16		83		0.994512
2721	66		33		0.997452

SSRs (tot: 2)

Start position	End position	Sequence	Length
204	217	GCA GCA GCA GCA GC	14
2600	2612	ATTT ATTT ATTT A	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain