SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene5547

Length: 1485 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene5547 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	hydrolase, hydrolyzing O-glycosyl compounds (EC:3.2.1.20)	-	-	67%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	36%
Blast2go	protein	-	-	76%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Glycoside hydrolase, family 31	IPR000322	-	0.0	76%
Blast2go	Glycoside hydrolase, superfamily	IPR017853	-	0.0	76%

Full-Lengther Next Prediction

Fln status: Putative C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LKI6

Fln msg: STOP codon was not found. Distance to subject end: 38 aas, your sequence is shorter than subject: 494 - 908

Fln protein: RAWWLKLISDFVSNGVDGIWNDMNEPAVFKTVTKTMPESNIHTGDEELGGCQKHSHYHNVYGMLMARSTYEGMLLANPQKRPFVLTRAGYIGSQRYAATWTGDNLSTWEHLHNSIPMALNLGLSGQPLSGPDIGGFAGNATPRLFARWMGIGTMLPFSRGHSEAQTTDHEPWVFGAECEKVCQLALQRRYRLLPHLYTLFYFAHTKGDLVASPLFFADWNDPNLRDVEDTFLLGQLLVSASTAREKGSDSKETVLPKGVWRRFDFDDNHPDLPLLYLGGGSIIPVGPCIQHTGEAKPTDTLTLLVALDEQGNAEGFLFEDGGDGYEYQQGQFLLTYYKAESSSSVVSIQVARTEGLWKRPRRKLNIQLLLGEGAKIEAWGHDGEKVEVEIPSAVEIAELVAESQKQDSLNIAQAKRPPDEQENYMRGAELSRIPVDLRSGDWHLKVVPWIGGRMISMAHIPSGTQWLHSRVEMNGYEEYSGIEYRSAGCSEEYT

Protein Length: 495

Fln nts: CTCGAGCATGGTGGTTGAAATTGATTTCTGATTTTGTTTCAAATGGTGTTGATGGTATATGGAATGATATGAATGAACCGGCTGTCTTCAAGACTGTGACAAAAACTATGCCTGAGAGTAATATTCATACAGGCGATGAAGAACTGGGTGGATGCCAGAAGCATTCTCATTATCACAATGTTTATGGCATGTTGATGGCAAGGTCCACATACGAAGGAATGTTGTTAGCAAATCCCCAAAAACGACCCTTTGTTCTAACTAGGGCTGGATATATAGGCAGTCAAAGGTATGCTGCAACATGGACAGGAGATAATCTTTCAACTTGGGAGCACCTACATAATAGTATTCCCATGGCCCTTAATCTGGGACTTAGTGGACAGCCATTGTCGGGACCTGATATTGGTGGCTTTGCTGGAAATGCAACACCTAGGTTATTTGCACGATGGATGGGAATTGGTACAATGCTTCCATTTTCTCGTGGCCATAGTGAAGCTCAAACAACTGACCATGAGCCATGGGTGTTTGGTGCCGAGTGTGAAAAAGTTTGTCAACTGGCACTTCAGAGGCGATATAGATTGCTACCCCACTTATACACACTCTTTTATTTTGCTCACACAAAAGGAGATCTAGTGGCATCTCCACTTTTTTTTGCTGATTGGAATGATCCAAATCTACGCGATGTAGAAGATACATTTCTACTGGGGCAACTTCTAGTCTCTGCCAGTACTGCACGAGAAAAGGGTTCAGATTCAAAAGAGACGGTACTGCCTAAGGGTGTCTGGAGGCGTTTTGACTTTGACGATAATCATCCTGATCTACCATTGCTGTATTTGGGCGGTGGATCTATTATTCCAGTAGGTCCATGTATTCAACACACAGGTGAAGCAAAGCCCACTGACACACTAACACTCCTTGTGGCCTTAGATGAACAAGGAAATGCTGAAGGATTTCTTTTTGAGGATGGTGGTGATGGATATGAATACCAGCAGGGGCAATTCCTTTTGACATATTATAAAGCTGAATCTTCATCATCTGTTGTTTCTATCCAAGTCGCTAGAACTGAAGGATTATGGAAAAGGCCAAGGCGTAAATTGAATATTCAATTATTGCTTGGTGAGGGTGCCAAGATAGAAGCATGGGGACATGATGGAGAAAAGGTTGAAGTTGAGATTCCTTCTGCGGTTGAGATTGCAGAGCTTGTAGCTGAAAGTCAAAAGCAAGACAGTTTAAATATAGCCCAAGCAAAGCGCCCTCCAGATGAACAAGAGAATTATATGAGGGGAGCAGAACTCTCTAGGATTCCTGTTGATCTTAGGAGTGGTGATTGGCATCTCAAAGTAGTTCCTTGGATTGGAGGTCGTATGATCTCCATGGCCCATATTCCCTCTGGTACCCAGTGGTTGCATAGCAGGGTTGAAATGAATGGGTACGAGGAATACAGTGGCATTGAGTATCGTTCTGCTGGTTGTTCTGAGGAATATACTG

Fln Alignment:

sp_v1.1_unigene5547___WWLKLISDFVSN-GVDGIWNDMNEPAVFKTVTKTMPESNIHTGDEELGGC----------------------------------QKHSHY--------HNVYGMLMARSTYEGML-LANPQKRPFVLTRAGYIGSQRYAATWTGDNLSTWEHLHNSIPMALNLGLSGQPLSGPDIGGFAGNATPRLFARWMGIGTMLPFSRGHSEAQTTDHEPWVFGAECEKVCQLALQRRYRLLPHLYTLFYFAHTKGDLVASPLFFADWNDPNLRDVEDTFLLGQLLVSASTAREKGSDSKETVLPKGVW
B8LKI6______________FWADEISRFHSMVPVDGLWIDMNEVSNFCSGKCTVPTNRSCPGTGLPWECCLDCTNITATQWDVPPYKINASGAGAPLGFKTIATSSVHYNGILEYDAHSLYGFSQAIATHKALQNLLN--KRPFVLTRSTFVGSGSYAAHWTGDNKATWEDLRYSISTILNFGMFGMPMVGADICGFYPDTTEELCGRWIQLGAFYPFSRDHSNLHSKRQELYLWDS-VAKSARKALGLRYRLLPYLYTLNYDAHTTGAPIARPLFFSFPQDPETYGVTKQFLLGP-GVLVSPVLYNSTTSVNAYFPKGSW

SNPs (tot: 26)

Position	A %	C %	G %	T %	Probability
452		42		57	1.0
478		14		85	0.993533
656	88		11		0.923564
662	22		77		0.999999
668	22		11		0.999999
678		25	75		1.0
681	25			75	1.0
683	75		25		1.0
685		25		75	1.0
690	28			71	1.0
692	71		28		1.0
713	66			33	1.0
766		14		85	0.993533
767		14	85		0.993533
768	14		85		0.993533
769		85		14	0.993533
830		40	60		1.0
878	83			16	0.994512
880	16	83			0.994512
881	83	16			0.994512
937		28	71		1.0
1005		11		88	0.923564
1267	90		10		0.90298
1322	90	10			0.90298
1323		10	90		0.90298
1324	90	10			0.90298

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain