SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene4968

Length: 2087 nt

Origin of reads: Biogeco_1 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene4968
TTGATTCTAGGCTACAGATTCTTCTTTGACATGTGTGATAAGAATGGAGATGGCCGAATATCAGAAGATGAGGTTAAGGAGGTTATTGTGCTGAGTGCTTCTGCAAACAAACTGGCAAAGCTGAAGGAGAATGCTGCAGAATATGCTGGTCTCATCATGGAGGAGCTTGATCCAGATCATTTAGGATACATTGAGATATGGCAGCTTGAAGCTCTGCTACAAGGCATGGTGAACACTTATGGGAACGAAGGATGTGTAAAGCACAGCAAAACTTTGAGTAAGACTATGATTCCCAAGCGTTGGAGAAGTCCCATTTACAGATTCCTTGAGGTGGCAAAGGATTTTGTCCACGAGAACTGGAAGCGCATCTGGATCATCTTACTGTGGATAGCCTTGAATGCTTGTCTGTTCACATGGAAATTTATGCAATACAAGCACAAAGCTGCATTCCAGGTGATGGGTTACTGTGTTTGTGTAGCCAAGGGGGCTGCTGAGACGTTGAAGCTTAACATGGCTCTCATTCTCTTCCCTGTATGTCGCAACACAATCACCTGGCTCAGAAAGACCAGGGCTAGTTCAATTGTCCCGTTTGATGACAATATCAACTTTCATAAGGTGATGGCGGTGGGAATTACAACTGGAGTATTCATTCATGGGGTGGTGCATCTCTCCTGCGACTTCCCCAGAATTGTTTCATGTCCTGACCTCAAGTTTATGGATACAATAGGACGTGGATTCCACTATAGAAAACCAACTTATCAAGATCTGATAACCAGCACAGTGGGCTTAACTGGAATTATTATCGTGTTCCTTATGGCCTTCTCCTTCACGCTGGCCACTCATAGTTTCAGGAGAAACGTAGTCAAACTGCCATGGCCTCTGCATAAGCTAGCTGGCTTCAATGCTTTCTGGTATGCTCATCATTTGTTTATTGCGGTCTACACCCTGCTTATCATCCACTCCATCTTCATATATCTCACACAAGAATGGTACCAGAAAACGGCTTGGATGTACATGGCCATACCCATACTTCTGTACATCGGCGAGCGATTGCTCAGATTTTTCCGTGAAAATACCAACAAAGTTAATCTCGTGAAGGCTGCCATATATCCTGGTAATGTTCTTGCTTTGCATATGACCAAGCCTATGGGATTCAAGTACAAAAGTGGGATGTATTTGTTTATAAAGTGTCCAGATGTCTCTCCATTTGAATGGCATCCTTTTCTATTACCTCAGCTCCCGGAGACGATTACTTGAGTGTCCACATCCGAACCATGGGTGATTGGACAACAGAACTTAAGAGGATATTTTCTAGGGTTTGTGAACCCCCTACTGCCAATAAAAGTGGTCTACTGAGGGCTGACACTACACTGGTAGCTGACAACGGAGTCCCAGTATGCAGATTTCCCAAGCTTTACATCGATGGACCTTATGGAGCTCCAGCCCAAAATTACAACAAATATGATATTCTGTTACTCGTGGGTTTGGGAATTGGGGCAACGCCTTTCATCAGCATTCTTAAAGATCTTCTGAACAACATCAAGTCTAACGAAGAACAGGCTGAAGCTGGAAATGATGTGAGCAAATTCAAACCCAGTCCAACACGAAGTCCTGAAGGAAGTAATTACAAAGGACGAGTACCGGCCCGGGCATACTTCTACTGGGTTACTAGAGAACAAGGATCCTTTGAATGGTTCAAAGGTGTCATGAACGAAGTGGCAGAGAGCGATCACAACAGAGTGATCGAAATGCACAATTATTTAACGAGCGTGTACGAAGAAGGCGACGCGCGTTCGGCTCTCATAGCCATGGTTCAATCCCTGCATCATGCCAAAAATGGATTCGACATTGTTTCAGGCAGCAGAATTCGAACACACTTTGCGAGGCCAAATTGGAACAGAGTCTTCACCCAACTGTCCACAGCCCACAAGGGTGCTCGAATAGGGGTATTTTACTGTGGGCCGCCTACTCTTACAAAGCAATTACGTTCACTTTCGCAGCAATTTACCCATAACTCAAGCACTCGTTTTGAATTTCACAAGGAGAATTTCTAGCAGAATGGAATTTTTTCCAGATTGTCATTCGTG

Ace file of the UniGene sp_v1.1_unigene4968 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	respiratory burst oxidase, putative (EC:1.6.3.1); K13447 respiratory burst oxidase [EC:1.6.3.- 1.11.1.-]	-	-	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	62%
Blast2go	respiratory burst oxidase	-	-	77%

Source	ECs	Term	Type	e value	Identity
AutoFact	Oxidoreductases, Acting on NADH or NADPH, With a oxygen as acceptor.	EC:1.6.3.-	-	0.0	54%
AutoFact	Oxidoreductases, Acting on a peroxide as acceptor, Peroxidases.	EC:1.11.1.-	-	0.0	54%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Glutathione metabolism	00480		0.0	54%

Source	InterPros	Term	Type	Identity
Blast2go	Cytochrome b245, heavy chain	IPR000778	-	77%
Blast2go	FAD-binding 8	IPR013112	-	77%
Blast2go	Ferric reductase, NAD binding	IPR013121	-	77%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABF5

Fln msg: Overlapping hits, possible frame ERROR between 1242 and 1221, your sequence is longer than subject: 675 - 358

Fln protein: MCDKNGDGRISEDEVKEVIVLSASANKLAKLKENAAEYAGLIMEELDPDHLGYIEIWQLEALLQGMVNTYGNEGCVKHSKTLSKTMIPKRWRSPIYRFLEVAKDFVHENWKRIWIILLWIALNACLFTWKFMQYKHKAAFQVMGYCVCVAKGAAETLKLNMALILFPVCRNTITWLRKTRASSIVPFDDNINFHKVMAVGITTGVFIHGVVHLSCDFPRIVSCPDLKFMDTIGRGFHYRKPTYQDLITSTVGLTGIIIVFLMAFSFTLATHSFRRNVVKLPWPLHKLAGFNAFWYAHHLFIAVYTLLIIHSIFIYLTQEWYQKTAWMYMAIPILLYIGERLLRFFRENTNKVNLVKAAIYPGNVLALHMTKPMGFKYKSGMYLFIKCPDVSPFEWHxxxxxxxxxDDYLSVHIRTMGDWTTELKRIFSRVCEPPTANKSGLLRADTTLVADNGVPVCRFPKLYIDGPYGAPAQNYNKYDILLLVGLGIGATPFISILKDLLNNIKSNEEQAEAGNDVSKFKPSPTRSPEGSNYKGRVPARAYFYWVTREQGSFEWFKGVMNEVAESDHNRVIEMHNYLTSVYEEGDARSALIAMVQSLHHAKNGFDIVSGSRIRTHFARPNWNRVFTQLSTAHKGARIGVFYCGPPTLTKQLRSLSQQFTHNSSTRFEFHKENF*

Protein Length: 676

Fln nts: TTGATTCTAGGCTACAGATTCTTCTTTGAC_-_ATGTGTGATAAGAATGGAGATGGCCGAATATCAGAAGATGAGGTTAAGGAGGTTATTGTGCTGAGTGCTTCTGCAAACAAACTGGCAAAGCTGAAGGAGAATGCTGCAGAATATGCTGGTCTCATCATGGAGGAGCTTGATCCAGATCATTTAGGATACATTGAGATATGGCAGCTTGAAGCTCTGCTACAAGGCATGGTGAACACTTATGGGAACGAAGGATGTGTAAAGCACAGCAAAACTTTGAGTAAGACTATGATTCCCAAGCGTTGGAGAAGTCCCATTTACAGATTCCTTGAGGTGGCAAAGGATTTTGTCCACGAGAACTGGAAGCGCATCTGGATCATCTTACTGTGGATAGCCTTGAATGCTTGTCTGTTCACATGGAAATTTATGCAATACAAGCACAAAGCTGCATTCCAGGTGATGGGTTACTGTGTTTGTGTAGCCAAGGGGGCTGCTGAGACGTTGAAGCTTAACATGGCTCTCATTCTCTTCCCTGTATGTCGCAACACAATCACCTGGCTCAGAAAGACCAGGGCTAGTTCAATTGTCCCGTTTGATGACAATATCAACTTTCATAAGGTGATGGCGGTGGGAATTACAACTGGAGTATTCATTCATGGGGTGGTGCATCTCTCCTGCGACTTCCCCAGAATTGTTTCATGTCCTGACCTCAAGTTTATGGATACAATAGGACGTGGATTCCACTATAGAAAACCAACTTATCAAGATCTGATAACCAGCACAGTGGGCTTAACTGGAATTATTATCGTGTTCCTTATGGCCTTCTCCTTCACGCTGGCCACTCATAGTTTCAGGAGAAACGTAGTCAAACTGCCATGGCCTCTGCATAAGCTAGCTGGCTTCAATGCTTTCTGGTATGCTCATCATTTGTTTATTGCGGTCTACACCCTGCTTATCATCCACTCCATCTTCATATATCTCACACAAGAATGGTACCAGAAAACGGCTTGGATGTACATGGCCATACCCATACTTCTGTACATCGGCGAGCGATTGCTCAGATTTTTCCGTGAAAATACCAACAAAGTTAATCTCGTGAAGGCTGCCATATATCCTGGTAATGTTCTTGCTTTGCATATGACCAAGCCTATGGGATTCAAGTACAAAAGTGGGATGTATTTGTTTATAAAGTGTCCAGATGTCTCTCCATTTGAATGGCATxxxxxxxxxxxxxxxxxxxxxxxxxxxGACGATTACTTGAGTGTCCACATCCGAACCATGGGTGATTGGACAACAGAACTTAAGAGGATATTTTCTAGGGTTTGTGAACCCCCTACTGCCAATAAAAGTGGTCTACTGAGGGCTGACACTACACTGGTAGCTGACAACGGAGTCCCAGTATGCAGATTTCCCAAGCTTTACATCGATGGACCTTATGGAGCTCCAGCCCAAAATTACAACAAATATGATATTCTGTTACTCGTGGGTTTGGGAATTGGGGCAACGCCTTTCATCAGCATTCTTAAAGATCTTCTGAACAACATCAAGTCTAACGAAGAACAGGCTGAAGCTGGAAATGATGTGAGCAAATTCAAACCCAGTCCAACACGAAGTCCTGAAGGAAGTAATTACAAAGGACGAGTACCGGCCCGGGCATACTTCTACTGGGTTACTAGAGAACAAGGATCCTTTGAATGGTTCAAAGGTGTCATGAACGAAGTGGCAGAGAGCGATCACAACAGAGTGATCGAAATGCACAATTATTTAACGAGCGTGTACGAAGAAGGCGACGCGCGTTCGGCTCTCATAGCCATGGTTCAATCCCTGCATCATGCCAAAAATGGATTCGACATTGTTTCAGGCAGCAGAATTCGAACACACTTTGCGAGGCCAAATTGGAACAGAGTCTTCACCCAACTGTCCACAGCCCACAAGGGTGCTCGAATAGGGGTATTTTACTGTGGGCCGCCTACTCTTACAAAGCAATTACGTTCACTTTCGCAGCAATTTACCCATAACTCAAGCACTCGTTTTGAATTTCACAAGGAGAATTTCTAG___CAGAATGGAATTTTTTCCAGATTGTCATTCGTG

Fln Alignment:

sp_v1.1_unigene4968___VYTLLIIHSIFIYLTQEWYQKTAWMYMAIPILLYIGERLLRFFRENTNKVNLVKAAIYPGNVLALHMTKPMGFKYKSGMYLFIKCPDVSPFEWHPxxxxxxxGDDYLSVHIRTMGDWTTELKRIFSRVCEPPTANKSGLLRADTTLVADNGVPVCRFPKLYIDGPYGAPAQNYNKYDILLLVGLGIGATPFISILKDLLNNIKSNEEQAEAGNDVSKFKPSPTRSPEGSNYKGRVPARAYFYWVTREQGSFEWFKGVMNEVAESDHNRVIEMHNYLTSVYEEGDARSALIAMVQSLHHAKNGFDIVSGSRIRTHFARPNWNRVFTQLSTAHKGARIGVFYCGPPTLTKQLRSLSQQFTHNSSTRFEFHKENF
D5ABF5______________VYTLLIVHGVFLYLTHKWYKKTTWMYLAVPVLLYGGERILRAFRSGSKPVQILKVAIYPGNVLTLHMSKPQGFRYKSGQYMFVNCPAVSSFEWHPxxxxxxxGDNYLSVHIRTLGDWTRELKNSFAEVCEPPMGGKSGLLRADYMQGKNNP----RFPRLLIDGPYGAPAQDYKKYDVLLLVGLGIGATPFISILKDIVNHMKVSEEERNIEN-VGKQKKKTFNT-----------VRAFFYWVTREEGSFDWFKGVMNEVAEIDQKGIIELHNYCTSVYEEGDARSALIAMLQALNHAKHGVDIVSGTRVCTHFAKPNWRNVFKSITLKHKDSRVGVFYCGPAALTDDLRQLSLDFTRKTSTRFDFHKENF

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
703	25		75		1.0
739	87		12		0.992554
835	33		66		1.0
1603		50	50		1.0
1621	66		33		0.997452
1977	20			20	0.99255
1978	80	20			0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain