SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene4916

Length: 1302 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene4916 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Unassigned protein	-	-	-
FL-Next	tr=Putative uncharacterized protein; Arabidopsis lyrata subsp. lyrata (Lyre-leaved rock-cress).	-	-	41%
Blast2go	hypothetical protein ARALYDRAFT_918735 [Arabidopsis lyrata subsp. lyrata]	-	-	64%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: tr_plants

Fln subject: D7ML57

Fln msg: Unexpected STOP codon at 3' end. Distance to subject end: 261 aas, No stop codon before M and low homology subject, your sequence is shorter than subject: 222 - 648

Fln protein: MSGPITTASMAKAMAEGQATIKHRKTSSSESLAWKDMFDGVAHLSPENHEKFFSRDIKSTDPAEKTVPPKENEAEGENSYETSQITRSPELRLALYIALAHGGLILSLAALYGVGKLLEEYWTPIQWAILCSMPLREIQGSLVEFWTQPLKLGLLETILALPVALCQAMVGTFNDLKAAICRVVRKSKKSKVQSSEENKNKNQQVGFSKLLRWLVSLGCSC*

Protein Length: 223

Fln nts: ATGGGATCCGCAATTCAAAACCTCCGAGAGCCTCGCAGAGGTCA_-_ATGTCAGGGCCGATCACGACAGCTTCAATGGCAAAGGCAATGGCGGAAGGACAAGCAACCATTAAGCACAGAAAAACGTCGAGCTCGGAATCCCTGGCATGGAAAGACATGTTCGATGGAGTGGCGCATCTCAGCCCAGAAAACCATGAGAAATTCTTTTCACGGGACATCAAGAGTACGGATCCCGCAGAGAAAACAGTTCCCCCGAAGGAAAATGAAGCAGAAGGTGAGAACAGCTACGAGACGTCACAGATAACTAGGAGCCCGGAGCTGCGTCTGGCTCTGTATATAGCACTGGCGCACGGGGGACTGATTCTGTCGTTAGCCGCTCTGTACGGCGTGGGAAAGCTGCTCGAGGAGTACTGGACGCCAATTCAATGGGCAATTCTGTGTTCCATGCCGCTGCGGGAGATACAGGGGTCGCTGGTTGAATTTTGGACCCAGCCGTTAAAACTCGGCCTTCTGGAGACCATCCTGGCGCTTCCCGTGGCTCTCTGTCAGGCAATGGTCGGGACCTTTAATGATCTGAAGGCCGCCATTTGCCGGGTTGTCAGAAAGAGCAAGAAGTCTAAGGTCCAGAGCTCAGAGGAAAACAAGAACAAGAATCAGCAGGTCGGGTTTTCGAAACTACTGAGATGGCTGGTTTCCTTGGGCTGTTCATGTTGACCTACGAGCGCCTCGGAGCGTGCGCGTGGGCGATATTTTCATTCGCGGGTTGCATTCTGTACGCCGCGGGCCGGACCGTCCACTTGCAGGACCCGAGCCCTGGTTCGCAAAAGCATGTGGCCTCAACGCTTGCGGCGATTTCGATGGCCCGCCGGCGAAGAGCAGATGATGATTCGCAGTTGCCGATTTGGAGCCGAGCTAGCCGAGGCCTGACCCAGTGCGTGCTCAAGCGCCTGCACACGCTGATAGCCCTTGGGCTTATTACGATGATGATCGTCGGATCCATGGCCGGAATTATGTTCTTTTCCTATAAAATAGGGCTGGAAAGCAGAGACGCGGTCATGTCCATCAAAGTTCATGTTCAGAAGAGTAATTATGCAGAGCGGGTGGGGCTTAAACAATGGATTAACGATAACAATATTCCAGAGTTGATGGAAACTTATTCAGTTAAATTTTACGAGACTGATTTCACAGCAGGTCGATTCGATTGCTCAGCAATACAATATGACTGAATTGGTGCAGTCTTTCAAGCATTTTGTGATCAAATCCAAGCCCAACGAGACTTCAACCACTTCGGCGCTGGCGATACCAC

Fln Alignment:

sp_v1.1_unigene4916___KTSSSESLAWKDMFDGVAHLSPENHEKFFSRDIKSTDPAEKTVPPKENEAEGENSYETSQITRSPELRLALYIALAHGGLILSLAALYGVGKLLEEYWTPIQWAILCSMPLREIQGSLVEFWTQPLKLGLLETILALPVALCQAMVGTFNDLKAAICXXXXXXXXXXXXXXXXXXXXNQQVGFSKLLRWLVSLG
D7ML57______________KSSIPTNLAWQEMFRSASSRKPQD------------PPSSSSSPPRKPSGDGSSSGKTSLSTADSQARLAMYIAMAHAGLVFAICVLYFVGKLLQEYLRPIQWAILCSIPLRGIQETLVDFWSEPLKLGLTEVVLAVPVSVFNVFIGSIVDIK-NLC-----FRVFLRRSKPKRTRKKNGTGFSKLVRWLVSFG

SNPs (tot: 27)

Position	A %	C %	G %	T %	Probability
117		20		80	0.995493
121		20	80		0.995493
124	80	20			0.995493
128	20	60	20		0.99255
130			80	20	0.995493
132		60	20		0.99255
133	40			60	1.0
135	25		75		0.996473
136			25	75	0.996473
137	75	25			0.996473
138	75	25			0.996473
141			25	75	0.996473
142			25	75	0.996473
144	75	25			0.996473
150	75	25			0.996473
151			25	75	0.996473
155			33	66	0.997452
184	25			75	0.996473
187	75			25	0.996473
330		50	50		1.0
418		33		66	0.997452
900			12	87	0.992554
901	87			12	0.992554
903	12			87	0.992554
905		87	12		0.992554
906	12			87	0.992554
914		42		57	1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain