SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene4641

Length: 2084 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene4641
CGTCGTGACCTTGGCTGTCACTCAAGTATTGGTCTACGGGTTATAGTGGATGTTGTCTACAACCATTTGCATGCAAGTGGACCAAATGATAAGGACTCTATCCTTGACAAGAAAAATCTATAGGTTGTTCCTGGTTATTATTTACGAAGAAACACAGATGGGCTTGTGGAAAACAGTACATGTGTGAACAACACCGCCAGTGAGCATTACATGGTGAATCGATTGATAATTGATGATCTTCTTCTATGGGCAACAGAGTATAAGGTTGATGGATTTCGGTTTGACCTTATGGGACATCTGATGAAAAATACCATGGTTAGTGCAAGAAATGCACTTGATAGGTTATCAAGGGAGAAAAATGGCGTGGATGGTACTAAGATTTACATGTATGGAGAAGGATGGGATTTTGGTGAGGTTGCACACAATGGACGAGGGATAAATGCATCCCAATTAAATCTCACGGGAACAGGGATTGGGAGTTTTAACGATCGCATACGTGACTCAGTTCTTGGTGGTTCTCCATTTGGTCCTCCTCTTCAACAAGGTTTTGTGACAGGCTTATCTTTACAGCCAAACAAGCACAACCATGGAAATAAGGATGATGTAACAAAGTATCTTGCATCAACAACTGATTGGATTCATGTTGGATTGGCTGGGAATTTAAGAGAGTTTGTATTCACTAACCATGAAGGACTAGAGGTAAAGGGATCAGAAGTGCAAACTCATGATGGAGTACCTGTTGGTTATGCAGGTAGTCCAAAAGAAACAATAAATTATGTTTCTGCACATGATAATGAAACGTTATTCGACATCATCATGCTAAAGGCTGCAAATGAAGTTCCATTGGATGAAAGGTGTAGGATAAATCATTTGGCGACAAGTATTGTGGCACTGTCACAGGGTATACCTTTTTTCCATGCAGGTGATGACATGTTACGCTCTAAATCTCTGGATCGCGATTCATATAACTCTGGTGATTGGTTCAACAGGTTAGACTTCACGTACGAATCAAATAATTGGGGAGTTGGATTGCCTCCAAAAGAAAGAATGGAAATAATTGGCCTTTGATGAAGGAACTTCTCGGAAATCCATCTTTGAGACCAGGGAAGCATCATATTCTTTCTGCTCTTGGAAACTTCCAAAACCTGTTGAGAATCAGACGGTCATCGCCTCTATTTCGGCTGGGGACAGTAAATGCTATTCAAGCAAGAGTTTGTTTCCACAACACTGGGCCATCATGGATCCCAGGGGCCATTGTTATGAGCATTAAGGATGGTGACAAAGGAATGCCTGGTTTAACTCAGCTTGATCCCTTGTACCGAAGGGTAGTTCTAATATTTAATGCCCGTCCCAGTGAATTTGTTGCTCCAATTTCTGAACTGAAATCATTGCCACTTACCCTCCATCCAATACAGGAGGCATCATCTGATGCACGGGTAAAAACTTCTTTCTATGATGCAAGCACTGGTACATTCAAGATTCCGCCAAGGACAACCTCTGTATTTGTGGAAGCCCGTTAGGCACGTGTCTCCTTTCACTGTTTCTCCATGAATAAGGAAATCAAAGCAGCTATAAAAGCACCCCACTGGTATTTACAGAAACATCATGTAAGTAGCTTCAGGAATATAATGGCATACATTTGGATTGTTTATTTGTGATGCACATCATTTAATTTATCAATTTTGTATTTCAAGACTGGTTATGTAAAATGAACTTTAGTTGGAAATCGGAACAGCAACTGTCAACTACGTTTATGGGGAATAAAACATTCTAAAAGATGCGAGGTTACTCAATTGTGGAGGGAGCATCTGGGTATTGTTTATATTCCTTAATTTAGTGATGGACAATCCCTTATTGACTTGTACCCAGTTTCTGTGAAATAATTGCCGTCGAATTTTCATACTTCTGTCAAAATCCAGGGATCTCACAAAAATTAGCTGCAATGTAATCTTTACGGAAAGGATAACCAATCCAACTCTCAATCACCAATATATGTTTCAGATATGAATGATTTTCGTTACGAGAACATCTAGCATCAATGAAGTGTAAATCACAAAGCACCAAGGTGATAGCTTTGTGTAACT

Ace file of the UniGene sp_v1.1_unigene4641 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pullulanase, putative n=1 Tax=Ricinus communis RepID=B9T3G1_RICCO	-	-	73%
FL-Next	sp=Pullulanase 1, chloroplastic; Arabidopsis thaliana (Mouse-ear cress).	-	-	71%
Blast2go	alpha- -glucosidase	-	-	81%

Source	GOs	Term	Type	e value	Identity
Blast2go	hydrolase activity	GO:0016787	Molecular Function	0.0	81%

Source	InterPros	Term	Type	Identity
Blast2go	Glycoside hydrolase, subgroup, catalytic domain	IPR013781	-	81%
Blast2go	Alpha amylase	IPR015902	-	81%
Blast2go	Glycoside hydrolase, superfamily	IPR017853	-	81%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: sp_plants

Fln subject: Q8GTR4

Fln msg: Overlapping hits, possible frame ERROR between 1053 and 1020, your sequence is shorter than subject: 507 - 965

Fln protein: RRDLGCHSSIGLRVIVDVVYNHLHASGPNDKDSILDKKNL*VVPGYYLRRNTDGLVENSTCVNNTASEHYMVNRLIIDDLLLWATEYKVDGFRFDLMGHLMKNTMVSARNALDRLSREKNGVDGTKIYMYGEGWDFGEVAHNGRGINASQLNLTGTGIGSFNDRIRDSVLGGSPFGPPLQQGFVTGLSLQPNKHNHGNKDDVTKYLASTTDWIHVGLAGNLREFVFTNHEGLEVKGSEVQTHDGVPVGYAGSPKETINYVSAHDNETLFDIIMLKAANEVPLDERCRINHLATSIVALSQGIPFFHAGDDMLRSKSLDRDSYNSGDWFNRLDFTYESNNxxxxxxxxxxxxxNWPLMKELLGNPSLRPGKHHILSALGNFQNLLRIRRSSPLFRLGTVNAIQARVCFHNTGPSWIPGAIVMSIKDGDKGMPGLTQLDPLYRRVVLIFNARPSEFVAPISELKSLPLTLHPIQEASSDARVKTSFYDASTGTFKIPPRTTSVFVEAR*

Protein Length: 508

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAGTATTGGTCTACGGGTTATAGTGGATGTTGTCTACAACCATTTGCATGCAAGTGGACCAAATGATAAGGACTCTATCCTTGACAAGAAAAATCTATAGGTTGTTCCTGGTTATTATTTACGAAGAAACACAGATGGGCTTGTGGAAAACAGTACATGTGTGAACAACACCGCCAGTGAGCATTACATGGTGAATCGATTGATAATTGATGATCTTCTTCTATGGGCAACAGAGTATAAGGTTGATGGATTTCGGTTTGACCTTATGGGACATCTGATGAAAAATACCATGGTTAGTGCAAGAAATGCACTTGATAGGTTATCAAGGGAGAAAAATGGCGTGGATGGTACTAAGATTTACATGTATGGAGAAGGATGGGATTTTGGTGAGGTTGCACACAATGGACGAGGGATAAATGCATCCCAATTAAATCTCACGGGAACAGGGATTGGGAGTTTTAACGATCGCATACGTGACTCAGTTCTTGGTGGTTCTCCATTTGGTCCTCCTCTTCAACAAGGTTTTGTGACAGGCTTATCTTTACAGCCAAACAAGCACAACCATGGAAATAAGGATGATGTAACAAAGTATCTTGCATCAACAACTGATTGGATTCATGTTGGATTGGCTGGGAATTTAAGAGAGTTTGTATTCACTAACCATGAAGGACTAGAGGTAAAGGGATCAGAAGTGCAAACTCATGATGGAGTACCTGTTGGTTATGCAGGTAGTCCAAAAGAAACAATAAATTATGTTTCTGCACATGATAATGAAACGTTATTCGACATCATCATGCTAAAGGCTGCAAATGAAGTTCCATTGGATGAAAGGTGTAGGATAAATCATTTGGCGACAAGTATTGTGGCACTGTCACAGGGTATACCTTTTTTCCATGCAGGTGATGACATGTTACGCTCTAAATCTCTGGATCGCGATTCATATAACTCTGGTGATTGGTTCAACAGGTTAGACTTCACGTACGAATCAAATAATxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAATTGGCCTTTGATGAAGGAACTTCTCGGAAATCCATCTTTGAGACCAGGGAAGCATCATATTCTTTCTGCTCTTGGAAACTTCCAAAACCTGTTGAGAATCAGACGGTCATCGCCTCTATTTCGGCTGGGGACAGTAAATGCTATTCAAGCAAGAGTTTGTTTCCACAACACTGGGCCATCATGGATCCCAGGGGCCATTGTTATGAGCATTAAGGATGGTGACAAAGGAATGCCTGGTTTAACTCAGCTTGATCCCTTGTACCGAAGGGTAGTTCTAATATTTAATGCCCGTCCCAGTGAATTTGTTGCTCCAATTTCTGAACTGAAATCATTGCCACTTACCCTCCATCCAATACAGGAGGCATCATCTGATGCACGGGTAAAAACTTCTTTCTATGATGCAAGCACTGGTACATTCAAGATTCCGCCAAGGACAACCTCTGTATTTGTGGAAGCCCGTTAGGCACGTGTCTCCTTTCACTGTTTCTCCATGAATAAGGAAATCAAAGCAGCTATAAAAGCACCCCACTGGTATTTACAGAAACATCATGTAAGTAGCTTCAGGAATATAATGGCATACATTTGGATTGTTTATTTGTGATGCACATCATTTAATTTATCAATTTTGTATTTCAAGACTGGTTATGTAAAATGAACTTTAGTTGGAAATCGGAACAGCAACTGTCAACTACGTTTATGGGGAATAAAACATTCTAAAAGATGCGAGGTTACTCAATTGTGGAGGGAGCATCTGGGTATTGTTTATATTCCTTAATTTAGTGATGGACAATCCCTTATTGACTTGTACCCAGTTTCTGTGAAATAATTGCCGTCGAATTTTCATACTTCTGTCAAAATCCAGGGATCTCACAAAAATTAGCTGCAATGTAATCTTTACGGAAAGGATAACCAATCCAACTCTCAATCACCAATATATGTTTCAGATATGAATGATTTTCGTTACGAGAACATCTAGCATCAATGAAGTGTAAATCACAAAGCACCAAGGTGATAGCTTTGTGTAACT

Fln Alignment:

sp_v1.1_unigene4641___GLRVIVDVVYNHLHASGPNDKDSILDKKNL*VVPGYYLRRNTDGLVENSTCVNNTASEHYMVNRLIIDDLLLWATEYKVDGFRFDLMGHLMKNTMVSARNALDRLSREKNGVDGTKIYMYGEGWDFGEVAHNGRGINASQLNLTGTGIGSFNDRIRDSVLGGSPFGPPLQQGFVTGLSLQPNKHNHGNKDDVTKYLASTTDWIHVGLAGNLREFVFTNHEGLEVKGSEVQTHDGVPVGYAGSPKETINYVSAHDNETLFDIIMLKAANEVPLDERCRINHLATSIVALSQGIPFFHAGDDMLRSKSLDRDSYNSGDWFNRLDFTYESNNWxxxxxxxxxxxNNWPLMKELLGNPSLRPGKHHILSALGNFQNLLRIRRSSPLFRLGTVNAIQARVCFHNTGPSWIPGAIVMSIKDGDKGMPGLTQLDPLYRRVVLIFNARPSEFVAPISELKSLPLTLHPIQEASSDARVKTSFYDASTGTFKIPPRTTSVFVEAR
Q8GTR4______________GLNVVLDVVYNHLHASGPHDKESVLDK----IVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWxxxxxxxxxxxHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESR

SNPs (tot: 112)

Position	A %	C %	G %	T %	Probability
258	12		87		0.992554
302	85			14	0.993533
304	14		85		0.993533
347		14		85	0.993533
348		14		85	0.993533
349	14		85		0.993533
350	14	85			0.993533
351			14	85	0.993533
354		14		85	0.993533
355	14	85			0.993533
359		14		85	0.993533
360			14	85	0.993533
361			16	83	0.994512
365	83		16		0.994512
366		83		16	0.994512
367			16	83	0.994512
369	83	16			0.994512
372		16		83	0.994512
376	16			83	0.994512
377			16	83	0.994512
378			16	83	0.994512
383		16		83	0.994512
384	83		16		0.994512
387		83	16		0.994512
399	80		20		0.995493
400	20	80			0.995493
401	80		20		0.995493
402	80			20	0.995493
403	80		20		0.995493
405		20		80	0.995493
406	20			80	0.995493
408	80	20			0.995493
409		20		80	0.995493
412	80		20		0.995493
413			20	80	0.995493
415	80	20			0.995493
417	80	20			0.995493
421		20		80	0.995493
425	25	75			0.996473
426	75		25		0.996473
428		75	25		0.996473
432	75		25		0.996473
433	75	25			0.996473
434		25		75	0.996473
437	75		25		0.996473
438	25	75			0.996473
674	90		10		0.90298
703		90	10		0.90298
707	10			90	0.90298
711	90	10			0.90298
712	90	10			0.90298
714	10			90	0.90298
715			10	90	0.90298
717			90	10	0.90298
718	90	10			0.90298
719	10		90		0.90298
721	90		10		0.90298
724	90		10		0.90298
747	75			25	1.0
752	87			12	0.992554
755	87		12		0.992554
756		87		12	0.992554
757			12	87	0.992554
760		87	12		0.992554
762		12		87	0.992554
763	12			87	0.992554
764	14	85			0.993533
770	85		14		0.993533
772		14	85		0.993533
793	16		16		0.988813
800			16	83	0.994512
803	16			83	0.994512
804	16			83	0.994512
805			16	83	0.994512
811		75	25		0.996473
815		75	25		0.996473
840			75	25	0.996473
894	75		25		0.996473
969	80			20	0.995493
970			20	80	0.995493
978	20			80	0.995493
979			20	80	0.995493
982	20	80			0.995493
983	20			80	0.995493
988	20			80	0.995493
990	80		20		0.995493
992	80	20			0.995493
1159	25	75			0.996473
1160	75		25		0.996473
1162		75	25		0.996473
1165	25	75			0.996473
1166			25	75	0.996473
1167		25	75		0.996473
1170	25			75	0.996473
1173	75			25	0.996473
1174	75	25			0.996473
1176	75	25			0.996473
1177	75		25		0.996473
1178	75			25	0.996473
1179		25	75		0.996473
1202	66	33			0.997452
1203	66	33			0.997452
1204		33		66	0.997452
1752		66	33		0.997452
1757		33		66	0.997452
1758		33	66		0.997452
1759	33	66			0.997452
1761			33	66	0.997452
1762			33	66	0.997452
1765	33			66	0.997452
1766		33		66	0.997452
1771			33	66	0.997452

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain