SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene4206

Length: 2207 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene4206
ATGGGGATTCTGCAAAATATTTGTCCTGTACAGATCTTAAAACTAAGGCGAGGGCGGGGCCGGGGTAAAGCACGAGCGTTGGTTAACAGCTCAGCGATGGCCGTCCTATCTCGCTACATCAAGTGGTGGATTGCATTGAATTGCTACATGACTTTGCGGTGTCCGCTTCCGAGGGTTTCCATAGAATTCTGCACTTCTGAGATAAATGGGTTGGGCTGTGATCTAGCGGTAGGCGAATACGACACCCAAGAAATTCCGTCCCTCCTGTCGCCTGGTTCTTGTCTGAAAAACACGATCGATGGCATTCCTGATATTGAAATTGTTCATCATTTGCGTTGGATTTACGGTGATCGCCGAAGGGAAATATGTGGAAGTACGACTGCAAGAATCAGCAGATGTTTCTCCAGACATAAGCAATGCATGCCAGGCTTCTCTGTTTCCGGACGACTGCAAGTCATCTTTGATGATCAGCTCTGTGGCGACCAAGCGAGGCGCTGGAGCTGTGGAAATAATCAAAGCAGCCATGGGTTTGTCCTCTGATGGGGTCACCCATTGTTATCATCTCTCCGAAGCTATTCTGTCTATTGCCCTTGCTTCTGCTAATTTCAACATGACAGTAGCTGCTCGCAATTGCCTGGAGACTCTGAGTGCTGCCATTGCAACCATTTCCAGAAGCATTGCTGCTCCTTTACAGCTGACAGTTAAAGACGCCCAAACCTGGATCAGCGCCGCTCTCACCTATCAATATGGTTGTTACTCTGCTTTCCACGGTCTCAATTCAACGCGACTGGTGAGAAACACAGCCGATCAGATTAATGGAGTGATGAAGATAACCAGCACCGCTCTTGGGATGCTGGACGCCTTGAATACTTATGGCCACAACATGAAATCTTGGAAACCTCCACAGACAGAGAGATCCAAGAACTTCTCAACTAGGTTTCCAGATCACCAGCAAAGCCAAGACCTTCTGGATGTGCTGCTCAAAGTGGGGGAACTACCGGTCCCTAACATAACAGTATCAAAGGTAGAAGGGGAGCTTGAGTCTGAGCAATATTCTTCCACCATTCAAGCTGCTGTAGACAATGCCCCTGATTACTCCGCGCAGAGGTTCATAATCTACATAAAAGCTGGGGTTTATAAAGAAATCGTGAGAATTCCACCGAGCAAGACGAATCTCATGTTTGTCGGAGATGGCATGGATCGAACTATCGTCACCGGATCATTGTCTGCCCAGATTCCCGGCGTCGGCACACACGGCTCTGCAACTGTCGGGGTGAACGCGGACGGTTTTGTGGCGCGGGACATTGCATTCGAGAATACTGCGGGGCCGGAGATGCATCAGGCTGTCGCCCTCAGAGTCGATAGCGATCTCTCAGCATTCGAGAGGTGCGCCTTTCTCGGACACCAGGACACCCTATACGCCCACGCCCTCCGCCAGTTTTATCGGAATTGCAGGATCGAAGGCAACGTCGACTTCATATTTGGAAACGCAGCGGCCATCTTCCACAACTGCTCCATTCTCGTCCGCTCTCGCCAGGTGCCGTCTAACCTTTCTGAAGCGAACCCCATAACTGCCCACGGGCGATTGGATCCGGGTCAGACTACTGGCTTCGTATTCGAGAATTGCAGCATCGATGGTACGGAGGAATACATGGCAGAATTCTACGGCAATCCGAAAATGCACAAAGCCTACCTGGGTCGGCCGTGGAAAGCGTATTCACGGACCATTTTCATGCGGTCCTACATGGGGCCAGTTATTCAGCCGGACGGGTGGCTGCCTTGGGATGGAACCTTTGCCCTGGACACGCTGTACTATGGAGAGTTTGAAAATTACGGGCCGGGTGCCGGCAATGTCTCTGCCAGAGTGCCATGGTCTAGTGAGATTCCTCCAGGAGCAGTGGGTATGTATTCTATCCAGAACTTCATCGAGGGAGACCTGTGGCTACCCCAGATTCATATGTGATTCCCATTTAGCTTGCTGTCTGTCAGCACCACCCTCTGAATCTTCAATATAGTAGTAATATAATGCTGCAAAAGACCATCTCTGGAAACTATTCATTGTAATTAGAGTTACGGTTGAGGCTGTTTGGCAGAGTTCTTTTTACATACATTCTCTGATTAGCTCTGTAATTATATGTATGATTATGCCTGTAAAAATTCTATTATCAAATTGTCACCAATGGATATATTGTTTTTCAGTTCACTTT

Ace file of the UniGene sp_v1.1_unigene4206

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectinesterase n=1 Tax=Picea sitchensis RepID=A9NUI7_PICSI	-	-	55%
FL-Next	sp=Pectinesterase; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	56%
Blast2go	pectinesterase family protein	-	-	63%

Source	GOs	Term	Type	e value	Identity
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	63%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NUI7

Fln msg: Unexpected STOP codon in 5 prime region,

Fln protein: MAFLILKLFIICVGFTVIAEGKYVEVRLQESADVSPDISNACQASLFPDDCKSSLMISSVATKRGAGAVEIIKAAMGLSSDGVTHCYHLSEAILSIALASANFNMTVAARNCLETLSAAIATISRSIAAPLQLTVKDAQTWISAALTYQYGCYSAFHGLNSTRLVRNTADQINGVMKITSTALGMLDALNTYGHNMKSWKPPQTERSKNFSTRFPDHQQSQDLLDVLLKVGELPVPNITVSKVEGELESEQYSSTIQAAVDNAPDYSAQRFIIYIKAGVYKEIVRIPPSKTNLMFVGDGMDRTIVTGSLSAQIPGVGTHGSATVGVNADGFVARDIAFENTAGPEMHQAVALRVDSDLSAFERCAFLGHQDTLYAHALRQFYRNCRIEGNVDFIFGNAAAIFHNCSILVRSRQVPSNLSEANPITAHGRLDPGQTTGFVFENCSIDGTEEYMAEFYGNPKMHKAYLGRPWKAYSRTIFMRSYMGPVIQPDGWLPWDGTFALDTLYYGEFENYGPGAGNVSARVPWSSEIPPGAVGMYSIQNFIEGDLWLPQIHM*

Protein Length: 556

Fln nts: ATGGGGATTCTGCAAAATATTTGTCCTGTACAGATCTTAAAACTAAGGCGAGGGCGGGGCCGGGGTAAAGCACGAGCGTTGGTTAACAGCTCAGCGATGGCCGTCCTATCTCGCTACATCAAGTGGTGGATTGCATTGAATTGCTACATGACTTTGCGGTGTCCGCTTCCGAGGGTTTCCATAGAATTCTGCACTTCTGAGATAAATGGGTTGGGCTGTGATCTAGCGGTAGGCGAATACGACACCCAAGAAATTCCGTCCCTCCTGTCGCCTGGTTCTTGTCTGAAAAACACGATCG_-_ATGGCATTCCTGATATTGAAATTGTTCATCATTTGCGTTGGATTTACGGTGATCGCCGAAGGGAAATATGTGGAAGTACGACTGCAAGAATCAGCAGATGTTTCTCCAGACATAAGCAATGCATGCCAGGCTTCTCTGTTTCCGGACGACTGCAAGTCATCTTTGATGATCAGCTCTGTGGCGACCAAGCGAGGCGCTGGAGCTGTGGAAATAATCAAAGCAGCCATGGGTTTGTCCTCTGATGGGGTCACCCATTGTTATCATCTCTCCGAAGCTATTCTGTCTATTGCCCTTGCTTCTGCTAATTTCAACATGACAGTAGCTGCTCGCAATTGCCTGGAGACTCTGAGTGCTGCCATTGCAACCATTTCCAGAAGCATTGCTGCTCCTTTACAGCTGACAGTTAAAGACGCCCAAACCTGGATCAGCGCCGCTCTCACCTATCAATATGGTTGTTACTCTGCTTTCCACGGTCTCAATTCAACGCGACTGGTGAGAAACACAGCCGATCAGATTAATGGAGTGATGAAGATAACCAGCACCGCTCTTGGGATGCTGGACGCCTTGAATACTTATGGCCACAACATGAAATCTTGGAAACCTCCACAGACAGAGAGATCCAAGAACTTCTCAACTAGGTTTCCAGATCACCAGCAAAGCCAAGACCTTCTGGATGTGCTGCTCAAAGTGGGGGAACTACCGGTCCCTAACATAACAGTATCAAAGGTAGAAGGGGAGCTTGAGTCTGAGCAATATTCTTCCACCATTCAAGCTGCTGTAGACAATGCCCCTGATTACTCCGCGCAGAGGTTCATAATCTACATAAAAGCTGGGGTTTATAAAGAAATCGTGAGAATTCCACCGAGCAAGACGAATCTCATGTTTGTCGGAGATGGCATGGATCGAACTATCGTCACCGGATCATTGTCTGCCCAGATTCCCGGCGTCGGCACACACGGCTCTGCAACTGTCGGGGTGAACGCGGACGGTTTTGTGGCGCGGGACATTGCATTCGAGAATACTGCGGGGCCGGAGATGCATCAGGCTGTCGCCCTCAGAGTCGATAGCGATCTCTCAGCATTCGAGAGGTGCGCCTTTCTCGGACACCAGGACACCCTATACGCCCACGCCCTCCGCCAGTTTTATCGGAATTGCAGGATCGAAGGCAACGTCGACTTCATATTTGGAAACGCAGCGGCCATCTTCCACAACTGCTCCATTCTCGTCCGCTCTCGCCAGGTGCCGTCTAACCTTTCTGAAGCGAACCCCATAACTGCCCACGGGCGATTGGATCCGGGTCAGACTACTGGCTTCGTATTCGAGAATTGCAGCATCGATGGTACGGAGGAATACATGGCAGAATTCTACGGCAATCCGAAAATGCACAAAGCCTACCTGGGTCGGCCGTGGAAAGCGTATTCACGGACCATTTTCATGCGGTCCTACATGGGGCCAGTTATTCAGCCGGACGGGTGGCTGCCTTGGGATGGAACCTTTGCCCTGGACACGCTGTACTATGGAGAGTTTGAAAATTACGGGCCGGGTGCCGGCAATGTCTCTGCCAGAGTGCCATGGTCTAGTGAGATTCCTCCAGGAGCAGTGGGTATGTATTCTATCCAGAACTTCATCGAGGGAGACCTGTGGCTACCCCAGATTCATATGTGATTCCCATTTAGCTTGCTGTCTGTCAGCACCACCCTCTGAATCTTCAATATAGTAGTAATATAATGCTGCAAAAGACCATCTCTGGAAACTATTCATTGTAATTAGAGTTACGGTTGAGGCTGTTTGGCAGAGTTCTTTTTACATACATTCTCTGATTAGCTCTGTAATTATATGTATGATTATGCCTGTAAAAATTCTATTATCAAATTGTCACCAATGGATATATTGTTTTTCAGTTCACTTT

Fln Alignment:

sp_v1.1_unigene4206___LILKLFIICVGFT---VIAEGKYVEVRLQESADVSPDISNACQASLFPDDCKSSLMISSVATKRGAGAVEIIKAAMGLSSDGVTHCYHLSEXXXXXXXXXXNFNMTVAARNCLETLXXXXXXXXXXXXXPLQ-LTVKDAQTWISAALTYQYGCYSAFHGLNSTRLVRNTADQINGVMKITSTALGMLDALNTYGHNMKSWKPPQTERSKNFSTRFPDHQQSQDLLDVLLKVGELPVPNITVSKVEGELESEQYSSTIQAAVDNAPDYSAQRFIIYIKAGVYKEIVRIPPSKTNLMFVGDGMDRTIVTGSLSA-QIPGV-GTHGSATVGVNADGFVARDIAFENTAGPEMHQAVALRVDSDLSAFERCAFLGHQDTLYAHALRQFYRNCRIEGNVDFIFGNAAAIFHNCSILVRSRQVPSNLSEANPITAHGRLDPGQTTGFVFENCSIDGTEEYMAEFYGNPKMHKAYLGRPWKAYSRTIFMRSYMGPVIQPDGWLPWDGTFALDTLYYGEFENYGPGAGNVSARVPWSSEIPPGAVGMYSIQNFIEGDLWLP
A9NUI7______________LITILLALCAQFSSANYLSAGSGHKKHLVKKSSASHSIDLACQASQYPDLCKSSLQANSNISEN-AGAEEIIGAAMVLSSDKTTQSYLHSKQLLNTSD---NRNLTGAVKDCLEFLEGSIRYIAKSRTQQLNPRNIKDVKIWMSAALSHQYDCSSALKYVNTTQMVGRSMQELVIVMNFTSNALSMVDALDTYGKDMVIWRPPKTERSSKLSSTADYSHHYNKIWDVL-EVDDL-VSDVTVSKDES-------SMSIQQAVNSAPDYSERRFVIRIKAGVYEEIVRIPPTKTNLMFVGDGMDRTVITGSMRVPSLPGVPSTYDSATVAVNADGFLARDIAFENAAGPVSQQAVALRVDSDLSAFYNCALLGHQDTLYTHTLRQFYRNCRIEGTVDFIFGNSAAIFENCLILVRPRQVNASKGSSDAVTAHGRTDPAQPTGFVFHNCTINGTEEYMKEYYSNPKIYKAYLGRPWKMYSRVIFMNSYLGELIVPEGWMPWTGDFALDTLYYGEYQNYGPGA-KVSGRVPWSNQIPKINAGKYSINSFIQGDEWLP

SNPs (tot: 32)

Position	A %	C %	G %	T %	Probability
177	16		83		0.994512
179			16	83	0.994512
180			16	83	0.994512
181		83	16		0.994512
182		83	16		0.994512
245		87		12	0.992554
246	87		12		0.992554
247		87	12		0.992554
248		87	12		0.992554
249		87	12		0.992554
333	10			90	0.90298
338			11	88	0.923564
339			11	88	0.923564
1151			10	90	0.90298
1311	40		60		1.0
1332	16		83		0.994512
1425		83	16		0.994512
1644	10		90		0.90298
1645			90	10	0.90298
1646	90		10		0.90298
2134		33		66	1.0
2158	72	9	18		0.999994
2165			10	80	0.959698
2167	88		11		0.991528
2168	11			88	0.991528
2169		88		11	0.991528
2171	88		11		0.991528
2172	88		11		0.991528
2173		12		87	0.992554
2174		12		87	0.992554
2183		40		60	1.0
2199		25		75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain