SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene3584

Length: 1523 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene3584 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	zinc finger (MYND type) family protein / F-box family protein	-	-	63%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	56%
Blast2go	zinc finger family protein	-	-	70%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Tetratricopeptide-like helical	IPR011990	-	0.0	70%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9T6

Fln msg: Separated hits, possible frame ERROR between 657 and 861, query STOP codon is far from subject stop. Distance to subject end: 16 aas,

Fln protein: MRTRRACYPSPDEAIAFAAGSCRQKRRLMSTGASSKGNDAFENPFDVLHDDLLISIMAALSTTANSPADLANAKMTCKRFCSAASNRKVLSQASMAALTVKASKWNEDSDKFLKLCADAGNREACYMLGMIRFYCLMNRGSGLGLMAEAAIASHPCALYSLAVIKFNGSGGFRKDKVLKAGVVLCAKAAAIGHLDAMRELGHCLQDGYGVKxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSDFGCSAASPANFHAINAFLVEWFALHPPAQGLRLCSHSQCGRPETRKHEFRRCSACGSVHYCSRACQALDWKMKHRYDCNPAENWDEQ*

Protein Length: 371

Fln nts: CTGAAACTCTGTTTGAACAAG_-_ATGCGTACCAGAAGAGCATGCTATCCGTCTCCTGATGAAGCAATCGCCTTCGCTGCGGGATCGTGCAGACAGAAGAGACGATTAATGTCAACTGGGGCTAGTTCGAAAGGAAATGACGCTTTCGAGAATCCGTTTGATGTTCTTCATGACGATCTCCTGATTTCGATAATGGCCGCCCTCAGCACTACAGCTAACTCTCCAGCCGATCTCGCAAATGCAAAAATGACATGTAAAAGATTCTGTTCGGCAGCATCCAATCGAAAGGTTCTTAGCCAGGCGTCCATGGCAGCCCTCACTGTAAAGGCTTCAAAATGGAACGAAGATTCAGACAAATTTTTGAAACTTTGTGCCGACGCAGGCAATCGAGAAGCCTGTTACATGCTTGGCATGATTCGATTCTATTGCCTGATGAACAGAGGATCGGGCTTAGGTTTAATGGCCGAAGCAGCAATTGCATCACACCCATGCGCCCTGTATTCTCTGGCCGTTATAAAATTCAATGGAAGCGGAGGATTCAGAAAGGACAAAGTCCTAAAGGCTGGAGTTGTTCTATGTGCCAAGGCTGCCGCCATAGGACACTTAGATGCGATGAGAGAGCTTGGCCACTGTTTGCAGGACGGGTATGGCGTTAAAAGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAAGCGATTTTGGGTGCAGCGCAGCATCGCCTGCAAATTTTCATGCAATAAACGCATTCCTGGTAGAATGGTTTGCATTGCATCCGCCGGCACAGGGGTTAAGACTCTGCTCTCATTCTCAATGCGGGAGGCCAGAGACCAGAAAACATGAATTTAGGAGATGTTCTGCATGTGGGTCTGTGCATTATTGCTCCCGGGCTTGCCAGGCTTTGGACTGGAAAATGAAGCACAGATATGACTGCAATCCTGCGGAAAATTGGGATGAACAGTAA___AAGTTGAAAATCGTTGAAGAAAGATGACCACGATATGACAATTTTTAAATAGTTTGTTTCTCTAGTGGCATCGTTAGTAAAAGGGTCTTCTCGGGCAAATTCTGACATGACCCTTGTGCGCAGAGAGAGAAACATAGGGGTTGGGCATTCCCGCTTGGAATACAATAATTAGCTGAGTCTTTGAATTATGTTCCAGCTCAACAACACTGTACTCCAAATTTGAAAATGATGAAAAAAAACCCCTATGTACAATTTCTTCGTAGAATCTCAGTGAGTTTATGAACTTTTTAGCGTTTCTGATGCATGAATCAACGATTAAGAAAGTATTGTACAGTTTGAGATCCATGGTTGATCTTTTTTGGTTATACTGTCTTGCAATGATTTCAAGTTTTA

Fln Alignment:

sp_v1.1_unigene3584___MRTRRA-CYPSPDEAIAFAAGSCRQKRRLMSTGASSKGNDAFENPFDVLHDDLLISIMAALSTTANSPADLANAKMTCKRFCSAASNRKVLSQASMAALTVKASKWNEDSDKFLKLCADAGNREACYMLGMIRFYCLMNRGSGLGLMAEAAIASHPCALYSLAVIKFNGSGGFRKDKVLKAGVVLCAKAAAIGHLDAMRELGHCLQDGYGVKRxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSDFGCSAASPANFHAINAFLVEWFALHPPAQGLRLCSHSQCGRPETRKHEFRRCSACGSVHYCSRACQALDWKMKHRYDCNPAENW
D5A9T6______________MQTRRGTCYSSPAPAAPSTGCRRESKRRRENSPCHKRKRQVRGNHFDDIPDDLLVSILSKLSSTSRRPADFISALLTCRRFHAMGFQPFVLVKASTSAFAVKASSWCEGSHRFIKKCADAGNVEACYTLGMIRFYCLQNRGRGASLMAKAAIASHASALYSLAIIQFNGSGGGKKDKDLKAGVALCARAAFLGHIDALRELGHCLQDGYGISKxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxSDFGCNVPPPES-HPAAKFMVEWFKKRPPCAGLRLCSYAGCGRPETRKHEFRRCSVCGTVNYCSRACQALDWKIRHKHTCMPIDDW

SNPs (tot: 42)

Position	A %	C %	G %	T %	Probability
440		72		27	1.0
548	90		10		0.90298
644		16		83	0.999991
671		18		81	0.999994
673		20		80	0.999996
719	11		88		0.923564
768	80			20	0.995493
772		80		20	0.995493
774	20			80	0.995493
775	80	20			0.995493
777			16	83	0.994512
779		16	83		0.994512
784		83	16		0.994512
790		16		83	0.994512
792	16	83			0.994512
793	83	16			0.994512
1025	80		20		0.995493
1046		33		66	1.0
1092		14		85	0.993533
1281	85			14	0.993533
1282			14	85	0.993533
1285	14			85	0.993533
1286		14		85	0.993533
1288		85		14	0.993533
1291			14	85	0.993533
1293	14		85		0.993533
1318	16			83	0.994512
1370	87			12	0.992554
1371	87		12		0.992554
1373		87	12		0.992554
1374			87	12	0.992554
1380	11			88	0.923564
1381		88	11		0.923564
1382		11	88		0.923564
1383	88	11			0.923564
1388		11	88		0.923564
1389		11		88	0.923564
1392		11		88	0.923564
1393			12	87	0.992554
1394	12			87	0.992554
1396		87	12		0.992554
1451	66		33		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain