SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene3310

Length: 1721 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene3310 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Atrophin-1 multi-domain protein	-	-	7.0e-06	24%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	85%
Blast2go	mucin-like protein	-	-	0.0	72%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Ribosomal protein S35, mitochondrial	IPR021036	-	0.0	72%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LM25

Fln msg: Separated hits, possible frame ERROR between 949 and 988, your sequence is shorter than subject: 363 - 397

Fln protein: TRQTLLSSSRNFLRILASANYNTGRNNDGKDGESDPKDPWDQAWVENPSPSSAGEEEDPWGMDSSWSTGLTEDHFEGEAVGRPVAEGEVVGPPATAASGRRERGRAGAGIDPNDEEWARLEKEYKENRKFVDSIDQKKTETFILLKQVREPGLRGSYLKDSEKQEMYKLHKENPEVYNVERLAKDFRIMRQRVHAILWLKEDEEEMEKRMGRPLDDAVEQLLDTCPELFKSADREFHVAELPLRPDFKVMPEDWDGTAKDPDEKLWEISQREDEMLYQEFVQRMNFNKAQIAGKVKCHKYSRRRPPEGWKFTIEExxxxxxxxxxxxxxRFVGLPDGSRRPLNDLEKMFLKRETNRPRRKIV*

Protein Length: 364

Fln nts: AACTCGCCAAACCCTCTTATCCTCCTCACGCAATTTCCTGCGAATCTTGGCCTCGGCAAACTACAACACGGGTAGAAACAACGACGGCAAAGACGGAGAATCGGACCCAAAGGATCCATGGGATCAGGCCTGGGTTGAAAACCCTAGCCCTAGTTCGGCAGGGGAGGAGGAAGATCCATGGGGAATGGACTCTTCCTGGTCGACAGGATTAACTGAAGATCACTTTGAGGGGGAAGCTGTCGGGCGCCCGGTCGCTGAAGGTGAAGTCGTGGGGCCACCGGCCACCGCCGCGAGCGGCAGAAGGGAGCGGGGCCGAGCCGGTGCGGGCATTGATCCTAATGATGAAGAATGGGCGAGGCTCGAAAAAGAATATAAAGAAAACAGAAAATTTGTGGACAGCATCGATCAGAAGAAGACTGAAACTTTTATTTTGTTGAAGCAGGTGAGGGAGCCGGGGCTCAGGGGTTCTTATTTGAAGGACTCGGAGAAGCAGGAGATGTACAAACTGCACAAGGAGAATCCCGAGGTTTACAATGTGGAAAGGTTGGCAAAGGATTTCAGGATCATGCGCCAGAGGGTCCACGCGATACTGTGGCTGAAGGAGGACGAGGAGGAAATGGAGAAGAGGATGGGCCGTCCACTTGATGATGCCGTGGAGCAGCTGCTGGATACGTGTCCGGAATTATTTAAATCTGCAGATCGCGAATTTCATGTAGCAGAGCTTCCTCTTCGTCCAGATTTCAAAGTTATGCCTGAGGACTGGGATGGCACTGCAAAAGATCCCGATGAAAAGTTGTGGGAGATATCTCAGAGGGAGGACGAAATGCTCTATCAGGAATTTGTGCAGCGTATGAATTTCAACAAAGCACAGATTGCTGGTAAGGTTAAATGTCATAAATATAGTCGAAGGAGACCTCCAGAAGGATGGAAGTTCACAATAGAGGAGCTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxGAGGTTTGTTGGTTTACCTGATGGGTCACGAAGACCTCTTAATGATTTGGAAAAGATGTTTTTGAAAAGAGAAACAAATCGGCCTCGACGCAAAATTGTATAACTGATGCTATGATATGCTCAAGTAAGACTTCATCCAATTTCCAGTAGGGTCAGATCTGCATAAGCTAGCAAAGAACCTGTACAGAATGAATGCCCATGGGTATATTTCAGAAACTTTGAATAATCAAATTTTGTTGACCTGTAGAGATGCATTGCTTTTTAATTCTCATCAATGGTGGGCTGTGATAGCGAGATGCACAAATATTTTTCAATAATGTGCTAAACATTAAATATGTTTTGGAGATATATGCAGCTGCTTATTTCCAACTCTAGGATTTTAGATTTCCCATTTTACATGGACTCGGGACGCTGCATCGCTATCCCTTCGGCGTGTTGCTCAGATAGAGTTTCCTACAATTTTGGCGTTTGTATTACTGCGAAACTGAAAGGCTGGCCAATAGACAAACCTGAACTTTTAGAGAAAGTGGGTAATAATTCTCTATGGCTAGATTTCGTATGGATCAGGTTGTGCTGTTTTTAAGAGGTGTTCACCCATTCAAAAACTTAACAATGAACATTCAGACACATTATAGATAGCACAAATCCATTATCTTGTTTTACAGCATCTATGATTGTTTAGTGAATCCTGTCACAGGAGTGGTAATTCTACAAAGGTGAGGTTAGATAATAAAC

Fln Alignment:

sp_v1.1_unigene3310___TRQTLLSSSRNFLRILASANYNTGRNNDGKDGESDPKDPWDQAWVENPSPS----------SAGEEEDPWGMDSSWSTGLTEDHFEGEAVGRPVAEGEVVGPPATXXXXXXXXXXXXXGIDPNDEEWARLEKEYKENRKFVDSIDQKKTETFILLKQVREPGLRGSYLKDSEKQEMYKLHKENPEVYNVERLAKDFRIMRQRVHAILWLKEDEEEMEKRMGRPLDDAVEQLLDTCPELFKSADREFHVAELPLRPDFKVMPEDWDGTAKDPDEKLWEISQREDEMLYQEFVQRMNFNKAQIAGKVKCHKYSRRRPPEGWKFTIEELxxxxxxxxxxxxxRFVGLPDGSRRPLNDLEKMFLKRETNRPRRKIV
B8LM25______________TPQTSLFSPGKFLQIFASKNYSAGRKNDGEGGESEPKDPWDQAWVENPSPSPPWEGKGEEEEEEEEEGAWGMDSSWSTGLTEDHFEGEAVGRPVAEGEVVGRPAPTEGGRKQQGRAGAGIDPDDEEWARLGREYRENKKFVDSIDQKMTETFILLKQVREPGLRGSYLKDSEKQEMYKLHKENPEVYTVERLAKDFRIMRQRVHAILWLKEDEEEMEKKMGRPLDDAVEQLLDTCPELFKSADREFHVAELPLRPDFKVMPEDWDGTAKDPDEKLWEISQREDEMLYQEFVQRMNFNKEQIAGKVKCHKYSRRRPPEGWKFTIEELxxxxxxxxxxxxxRFVGLPDGSRRPLNELEKMFLKRETNRPRRKIV

SNPs (tot: 62)

Position	A %	C %	G %	T %	Probability
187	75		25		0.996473
228		25		75	0.996473
369			87	12	0.992554
373	87		12		0.992554
375	87		12		0.992554
388			12	87	0.992554
389	87		12		0.992554
392	14			85	0.993533
394		85	14		0.993533
396	85	14			0.993533
397	85		14		0.993533
1227		81		18	0.999994
1228			18	72	0.999994
1229	18	81			0.999994
1230	9	72	18		0.999994
1233	20	80			0.999996
1234		20		80	0.999996
1235	20			80	0.999996
1236		80	20		0.999996
1240	20		80		0.999996
1246			20	80	0.999996
1247	20			80	0.999996
1249	80		20		0.999996
1324		83		16	0.994512
1352	83	16			0.994512
1353		16		83	0.994512
1354		16		83	0.994512
1355	16	83			0.994512
1376		87	12		0.992554
1377	12			87	0.992554
1379		87	12		0.992554
1382		87	12		0.992554
1384			25	75	1.0
1385		12		87	0.992554
1386	87		12		0.992554
1387	25		75		1.0
1388		12	87		0.992554
1389	87		12		0.992554
1390			12	87	0.992554
1392	87	12			0.992554
1393	87	12			0.992554
1394	12		12	75	0.975346
1395	12		87		0.992554
1396		87	12		0.992554
1397		75	12	12	0.975346
1399	14		85		0.993533
1401	85		14		0.993533
1402	14	85			0.993533
1413		83		16	0.994512
1414		83	16		0.994512
1415	16		83		0.994512
1416		83	16		0.994512
1418		83	16		0.994512
1419	16	83			0.994512
1421	16			83	0.994512
1422			16	83	0.994512
1424		16		83	0.994512
1425	16		83		0.994512
1426	16	83			0.994512
1427		83	16		0.994512
1432	16		83		0.994512
1435	16		83		0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain