SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene3258

Length: 1900 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene3258

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	DNA binding protein, putative	-	-	3.00004e-41	41%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	60%
Blast2go	dna binding	-	-	0.0	67%

Source	GOs	Term	Type	e value	Identity
Blast2go	cytosol	GO:0005829	Cellular Component	0.0	67%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Domain of unknown function DUF296	IPR005175	-	0.0	67%
Blast2go	AT hook, DNA-binding motif	IPR017956	-	0.0	67%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5A9Z4

Fln msg: Separated hits, possible frame ERROR between 1137 and 1143, query STOP codon is far from subject stop. Distance to subject end: 28 aas,

Fln protein: MNERRDFPGLHTSGPTSFLHPGFQQHHHLHGSTNLNAAAAANNNAISNVNPFYIPQGGIASNATLRFMSSPPSSLAIGNANPNNSPPEGPAASPGPHNPLPPRMGMNMGFRSEPLKRKRGRPRKYSSDGVGLGLSPLTPSSQSLSEKRARGSGSGAGSNKKVQLAALGSSGHGFTPHIITIEDGEDVSKKISAFTQQAPWAVCVLSANGTISSVTLHSGMSGGNVTYEGKYEILSLSGSFVLNEAGGKQTRTGKLTVAxxxGTDGSVVGGEVAGLLIAASPVQVVVGTFQADAKKGFGRTENSEPSGATLPASISGHPPSPVGLQSRTEGFGGLRTDEGSYSEHATGGGGYSLNSEIMHNMNMQSMDWGPQFDAEARSNAEATMTGGS*

Protein Length: 390

Fln nts: ACGTCGTGACCTTGGCTGTCACTCAATTTTTTTAATGCCCTGCGGATCTTTCTGTCTGAGCTTTTACACCACTGTCTACTCAGAAGAGCAACATAATTTTTTTGTCCTCCCGCGGTGGTGGACGGTTCGAGCCCCCGCGGCGGCTCTCAGCATTCGAAACGTTACAATCCGGTGGCCGCAGCAGCTAAGATCAAGATCAGCTTCTTTTCACACTAAAATTTACTCTTATTCAGATCTGGAATCTCTGCTGCTTTCAGTAATGCTCTAGAAAACACACAAGAAATTCCATATAACTACACTGAGATTTATAAAGAAATTCAAGCCTGTAAGCTGAATTAAGCAGAGTACAGTAGAGTATTT_-_ATGAATGAAAGGAGAGATTTCCCCGGCCTTCATACTTCAGGGCCGACTTCATTCTTACACCCAGGATTCCAACAGCATCATCACCTCCACGGCTCCACGAATCTCAATGCTGCCGCTGCTGCTAATAACAATGCTATTTCTAATGTCAATCCATTCTACATCCCACAGGGAGGCATTGCCAGTAACGCTACACTGCGCTTCATGTCCAGTCCTCCTTCATCTTTAGCAATTGGCAATGCCAATCCAAATAATTCTCCTCCAGAAGGGCCTGCTGCTTCACCGGGTCCTCATAATCCATTACCTCCTAGGATGGGAATGAATATGGGGTTTCGTTCAGAGCCTTTGAAGAGAAAGAGAGGAAGACCCAGGAAATACAGTAGTGATGGAGTAGGTTTGGGACTATCTCCATTGACTCCCAGCTCTCAGTCATTATCCGAAAAACGAGCCCGCGGCTCCGGCTCCGGCGCCGGCTCCAACAAGAAAGTTCAGTTGGCTGCACTTGGGAGTTCGGGTCATGGATTTACTCCACACATCATCACAATTGAAGACGGGGAGGATGTGTCGAAGAAGATATCAGCGTTTACGCAGCAAGCACCTTGGGCAGTGTGTGTGCTCTCAGCTAATGGAACAATTTCAAGTGTGACTCTTCATTCAGGGATGTCAGGTGGCAATGTAACGTATGAGGGAAAATATGAAATTCTTTCATTATCTGGCTCTTTTGTACTAAATGAAGCTGGAGGAAAGCAAACACGAACTGGTAAGCTGACTGTAGCTCTxxxxxxAGGGACAGATGGCAGTGTTGTTGGTGGTGAAGTGGCAGGTCTTCTTATAGCTGCAAGTCCTGTACAGGTAGTTGTTGGTACTTTCCAAGCTGATGCGAAGAAGGGCTTTGGACGAACTGAAAATAGCGAGCCTTCCGGTGCCACTTTGCCAGCGTCTATATCAGGGCATCCGCCTTCCCCAGTAGGTTTACAGTCAAGGACCGAGGGTTTTGGTGGCCTGAGAACAGATGAAGGGAGCTATTCTGAACATGCTACTGGAGGAGGTGGTTATAGTCTGAATTCTGAGATCATGCATAATATGAATATGCAGTCCATGGACTGGGGGCCTCAGTTTGACGCTGAGGCAAGGAGTAATGCTGAAGCAACAATGACAGGTGGGTCTTGA___CTAAATGTTTGTGATCAGTTGTGATCTTGGGCATTTTTTTTTAGGACAATTACGGTAGGCTTCTTTGACAAGTTTGATCTTAACCACAGATGATGTCTTAATGGCACGTGCATGTATATTCTTGAAACATCTCCTAGAAGGGGTGTAATCTCTGCAATTGTTATTTCCAACATTACCAAATATTACTCTCCCACTTCAAATTTCTCGGGTGTTGAGAGGGTTTTAATATCTCATTCAAAATGCAAATTTATAACTTTGGTAGGGTTATATCAGGAACAATAAATTTTGGGTTTCTTTATTTAATGACATTCATGTGCTCTTTTCATGGTGAATTTTTTCTGGCAAACATGATCTTGAATAAAATGCCTTTGTCC

Fln Alignment:

sp_v1.1_unigene3258___SEPLKRKRGRPRKYSSD--GVXXXXXXXXXXXXXXXEKXXXXXXXXXXXNKKVQLAALGSSGHGFTPHIITIEDGEDVSKKISAFTQQAPWAVCVLSANGTISSVTLHS-GMSGGNVTYEGKYEILSLSGSFVLNEAGGKQTRTGKLTVALxxGTDGSVVGGEVAGLLIAASPVQVVVGTFQADAKKGFGRTENSEPSGATLPASISGHPPSP-VGLQSRTEGFGGLRTDEGSYSEHATGGGGYSLNSEIMHNMNMQSMDW-GPQFDAEARSNAEATMTG
D5A9Z4______________SETLKRKRGRPRKYGTDVDGFGNVGLGLSSPSSPFSDKKGRGS------GKKAQMVALGCAGHGFIPHVITIAAGEDVCKKIMAFMQHGPWAVCVLSANGAISNVTLRQPAMSGGTVTYEGRFEILSLSGSFLLTDTGGTHTRTGGLSVSLxxGSDGRVIGGGVGGLLMAASPVQVVVGTFLVDNKKDLGRVGNGEPSMGTLSGSMSGGSPSPSIAVQSRAETSAGTNLRKGGEGEQNTGGGTYNMQPQVLTMMSLQSMDWGGAHFEAETKSDTETALTG

SNPs (tot: 6)

Position	A %	C %	G %	T %	Probability
1095	16		83		0.999991
1116	88			11	0.923564
1439	96		3		0.993591
1440	96		3		0.993591
1441			4	96	0.99411
1903		66	33		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain