SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene3058

Length: 2020 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene3058
ATGGGGACCAACATCAATATTCGTGTGACCTTGGCTGTCACGTCGTAATAAAGTCGAAGATGGGGATTGAGATTCAACAAACTCTTGTTGGCCTAGTGGCGAAAGAAGATTAGGCACCTGGGCACAATCGAAGGGAGCTCCGGAACATTGCTTGGGACACTAGGTGGTGTGATATAATGGCATTCATTTCAGCATATCGACGCCATCTATCTATATCTACTGCATCTTCCTTGATGCATGGCATATCATTTACATACAGCAGATCACTTTCGACGGCTTTTAGAGAGGAGAGGGATACATTTGGTCCCATCAATGTACCGGCAGACAAATTATGGGGTGCACAGACTCAGCGGTCACTGCAAAACTTTGAAATTGGTGGAGAGCGTGAGCAGATGCCTGAACAAATTATACAATCATTTGGTGTTCTGAAAAAATGCGCTGCTAAGGTTAATATGGAGTATGGTCTTGACCCAACCATTGGGAAAGCGATAATGCAAGCTGCTCAAGAAGTTGCTGAAGGAAAGCTGAACAGTCATTTCCCTTTGGTCATTTGGCAAACTGGTAGTGGGACTCAAAGTAACATGAACGCTAACGAGGTGATTGCAAATCGTGCTGCTGAAATTCTTGGAAAGAAACGTGGTGAAAAATGTGTACATCCAAATGACCATGTCAATATGTCTCAATCTTCTAATGATACATTTCCCACTGTTATGCACATTGCTGCAGCAGTGGAAATGAACTCAGAATTCATACCAAGCTTACGGCAGCTTCTTTCTACATTGGAAGCTAAGAGCAATGAATTTAAGGATATAGTGAAAATTGGACGTACACATACCCAAGATGCAACACCGCTAACTCTTGGTCAAGAGTTTAGTGGTTATGCTACCCAGGTGAAATACGGCATAGAACGTGTTACAAATTCATTGCCTCGTATATACCAGCTTGCACAAGGAGGGACTGCAGTTGGAACTGGTCTCAATACTAAAAAAGGGTTTGACATTAAAATTGCTGCAGCTGTTGCTGAAGAAACACAGCTACCATTTATTACAGCTGAGAACAAGTTTGAAGCTTTGGCAGCACATGATGCTTTTGTTGAAACCAGTGGAGCTGTGAACACTGTTTCAGTTTCTCTAATGAAGATAGCAAATGATATACGTTTCCTAGGAAGTGGGCCTCGCTGTGGACTAGGTGAACTGCTATTACCTGAAAACGAACCTGGCAGCAGCATCATGCCTGGAAAGGTGAACCCTACTCAATGTGAGGCTTTGACAATGGTTTGTGCTCAGGTTATGGGAAACCATGTTGCAATTACAGTAGGGGGATCAAATGGTCATTTTGAGCTTAATGTTTTTAAGCCATTGATCGCAAGTGCTCTACTTCGGTCTTTGACATTGCTAGGAGATGCTGCAGCTTCATTTGAAAAGAATTGTGTAAGGGGAATTCAGGCCAACCATCGAAGAATAGGACAGCTTGTCCGAGAGTCACTGATGCTTGTTACAGCTCTGAATCCTAAAATTGGGTATGACAAAGCAGCAGCAACTGCAAAAAAGGCCCATAAAGAGGGATCAACCCTGAAGGAAGCTGCACTGGAGCTTGGAGTTCTGACGTCTGAAGAGTTTGATGAATTGGTCGTGCCGGAGAAGATGATTGGACCATCAGATTAAGTTTTGTATATTTTATTTGTTTTGGCATTTACTGCCATAAAATTTGATTGTCAATGATGTGAACATCATAAATAAATAAGAGGCATCAACTGTCAATTTTGATCTGTCCCGACAGTGTTCCTCCATTTTCATGAAAGCTGTTCGATCAAGTTTACGAGTTAAGTGTTCAGCTTTCAGTTATTCAATAAAACTTGCTCTTTCCATCCGTTGAGTTCATTTAAGTTGCTTGTAGTGTGGAGCATGTTTCCTATGTAGCTTATATGCGTTTTTAAAATATTATACTTCAGATAATTATTTAATAATGTCAACACGCCAACTATAATTGCGCATTACTAATTCAAGGAGTTTTTACCTGC

Ace file of the UniGene sp_v1.1_unigene3058

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	FUM1; FUM1 (FUMARASE 1); catalytic/ fumarate hydratase (EC:4.2.1.2); K01679 fumarate hydratase, class II [EC:4.2.1.2]	-	-	79%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	98%
Blast2go	fum1 (fumarase 1) catalytic fumarate hydratase	-	-	91%

Source	ECs	Term	Type	e value	Identity
AutoFact	Fumarate hydratase.	EC:4.2.1.2	-	0.0	79%

Source	KEGGs	Term	Identity
AutoFact	Citrate cycle (TCA cycle)	00020	79%
AutoFact	Carbon fixation pathways in prokaryotes	00720	79%
AutoFact	Biosynthesis of phenylpropanoids	01061	79%
AutoFact	Biosynthesis of terpenoids and steroids	01062	79%
AutoFact	Biosynthesis of alkaloids derived from shikimate pathway	01063	79%
AutoFact	Biosynthesis of alkaloids derived from terpenoid and polyketide	01066	79%
AutoFact	Biosynthesis of plant hormones	01070	79%
AutoFact	Metabolic pathways	01100	79%
AutoFact	Biosynthesis of secondary metabolites	01110	79%

Source	GOs	Term	Type	Identity
Blast2go	response to oxidative stress	GO:0006979	Biological Process	91%
Blast2go	pollen tube development	GO:0048868	Biological Process	91%
Blast2go	response to salt stress	GO:0009651	Biological Process	91%
Blast2go	protein binding	GO:0005515	Molecular Function	91%
Blast2go	catalytic activity	GO:0003824	Molecular Function	91%
Blast2go	mitochondrion	GO:0005739	Cellular Component	91%

Source	InterPros	Term	Type	Identity
Blast2go	Fumarate lyase	IPR000362	-	91%
Blast2go	Fumarate hydratase, class II	IPR005677	-	91%
Blast2go	L-Aspartase-like	IPR008948	-	91%
Blast2go	Fumarase C, C-terminal	IPR018951	-	91%
Blast2go	Fumarate lyase, conserved site	IPR020557	-	91%
Blast2go	Lyase 1, N-terminal	IPR022761	-	91%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NVX1

Fln msg:

Fln protein: MAFISAYRRHLSISTASSLMHGISFTYSRSLSTAFREERDTFGPINVPADKLWGAQTQRSLQNFEIGGEREQMPEQIIQSFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNSHFPLVIWQTGSGTQSNMNANEVIANRAAEILGKKRGEKCVHPNDHVNMSQSSNDTFPTVMHIAAAVEMNSEFIPSLRQLLSTLEAKSNEFKDIVKIGRTHTQDATPLTLGQEFSGYATQVKYGIERVTNSLPRIYQLAQGGTAVGTGLNTKKGFDIKIAAAVAEETQLPFITAENKFEALAAHDAFVETSGAVNTVSVSLMKIANDIRFLGSGPRCGLGELLLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPLIASALLRSLTLLGDAAASFEKNCVRGIQANHRRIGQLVRESLMLVTALNPKIGYDKAAATAKKAHKEGSTLKEAALELGVLTSEEFDELVVPEKMIGPSD*

Protein Length: 497

Fln nts: ATGGGGACCAACATCAATATTCGTGTGACCTTGGCTGTCACGTCGTAATAAAGTCGAAGATGGGGATTGAGATTCAACAAACTCTTGTTGGCCTAGTGGCGAAAGAAGATTAGGCACCTGGGCACAATCGAAGGGAGCTCCGGAACATTGCTTGGGACACTAGGTGGTGTGATATA_-_ATGGCATTCATTTCAGCATATCGACGCCATCTATCTATATCTACTGCATCTTCCTTGATGCATGGCATATCATTTACATACAGCAGATCACTTTCGACGGCTTTTAGAGAGGAGAGGGATACATTTGGTCCCATCAATGTACCGGCAGACAAATTATGGGGTGCACAGACTCAGCGGTCACTGCAAAACTTTGAAATTGGTGGAGAGCGTGAGCAGATGCCTGAACAAATTATACAATCATTTGGTGTTCTGAAAAAATGCGCTGCTAAGGTTAATATGGAGTATGGTCTTGACCCAACCATTGGGAAAGCGATAATGCAAGCTGCTCAAGAAGTTGCTGAAGGAAAGCTGAACAGTCATTTCCCTTTGGTCATTTGGCAAACTGGTAGTGGGACTCAAAGTAACATGAACGCTAACGAGGTGATTGCAAATCGTGCTGCTGAAATTCTTGGAAAGAAACGTGGTGAAAAATGTGTACATCCAAATGACCATGTCAATATGTCTCAATCTTCTAATGATACATTTCCCACTGTTATGCACATTGCTGCAGCAGTGGAAATGAACTCAGAATTCATACCAAGCTTACGGCAGCTTCTTTCTACATTGGAAGCTAAGAGCAATGAATTTAAGGATATAGTGAAAATTGGACGTACACATACCCAAGATGCAACACCGCTAACTCTTGGTCAAGAGTTTAGTGGTTATGCTACCCAGGTGAAATACGGCATAGAACGTGTTACAAATTCATTGCCTCGTATATACCAGCTTGCACAAGGAGGGACTGCAGTTGGAACTGGTCTCAATACTAAAAAAGGGTTTGACATTAAAATTGCTGCAGCTGTTGCTGAAGAAACACAGCTACCATTTATTACAGCTGAGAACAAGTTTGAAGCTTTGGCAGCACATGATGCTTTTGTTGAAACCAGTGGAGCTGTGAACACTGTTTCAGTTTCTCTAATGAAGATAGCAAATGATATACGTTTCCTAGGAAGTGGGCCTCGCTGTGGACTAGGTGAACTGCTATTACCTGAAAACGAACCTGGCAGCAGCATCATGCCTGGAAAGGTGAACCCTACTCAATGTGAGGCTTTGACAATGGTTTGTGCTCAGGTTATGGGAAACCATGTTGCAATTACAGTAGGGGGATCAAATGGTCATTTTGAGCTTAATGTTTTTAAGCCATTGATCGCAAGTGCTCTACTTCGGTCTTTGACATTGCTAGGAGATGCTGCAGCTTCATTTGAAAAGAATTGTGTAAGGGGAATTCAGGCCAACCATCGAAGAATAGGACAGCTTGTCCGAGAGTCACTGATGCTTGTTACAGCTCTGAATCCTAAAATTGGGTATGACAAAGCAGCAGCAACTGCAAAAAAGGCCCATAAAGAGGGATCAACCCTGAAGGAAGCTGCACTGGAGCTTGGAGTTCTGACGTCTGAAGAGTTTGATGAATTGGTCGTGCCGGAGAAGATGATTGGACCATCAGATTAA___GTTTTGTATATTTTATTTGTTTTGGCATTTACTGCCATAAAATTTGATTGTCAATGATGTGAACATCATAAATAAATAAGAGGCATCAACTGTCAATTTTGATCTGTCCCGACAGTGTTCCTCCATTTTCATGAAAGCTGTTCGATCAAGTTTACGAGTTAAGTGTTCAGCTTTCAGTTATTCAATAAAACTTGCTCTTTCCATCCGTTGAGTTCATTTAAGTTGCTTGTAGTGTGGAGCATGTTTCCTATGTAGCTTATATGCGTTTTTAAAATATTATACTTCAGATAATTATTTAATAATGTCAACACGCCAACTATAATTGCGCATTACTAATTCAAGGAGTTTTTACCTGC

Fln Alignment:

sp_v1.1_unigene3058___MAFISAYRRHLSISTASSLMHGISFTYSRSLSTAFREERDTFGPINVPADKLWGAQTQRSLQNFEIGGEREQMPEQIIQSFGVLKKCAAKVNMEYGLDPTIGKAIMQAAQEVAEGKLNSHFPLVIWQTGSGTQSNMNANEVIANRAAEILGKKRGEKCVHPNDHVNMSQSSNDTFPTVMHIAAAVEMNSEFIPSLRQLLSTLEAKSNEFKDIVKIGRTHTQDATPLTLGQEFSGYATQVKYGIERVTNSLPRIYQLAQGGTAVGTGLNTKKGFDIKIAAAVAEETQLPFITAENKFEALAAHDAFVETSGAVNTVSVSLMKIANDIRFLGSGPRCXXXXXXXXXXXXXSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPLIASALLRSLTLLGDAAASFEKNCVRGIQANHRRIGQLVRESLMLVTALNPKIGYDXXXXXXXXXXXEGSTLKEAALELGVLTSEEFDELVVPEKMIGPSD
A9NVX1______________MAIISAYRRHLSISTASSLMHNISFTYSRSLSTAFREERDTFGPINVPADKLWGAQTQRSLQNFEIGGEREQMPEQIIQSFGVLKKCAAKVNMDYGLDPTIGKAIMQAAQEVAEGKLNSHFPLVIWQTGSGTQSNMNANEVIANRAAEILGRKRGEKCVHPNDHVNMSQSSNDTFPTVMHIAAAVEMNSKFIPSLRQLHSTLEAKSNEFKDIVKIGRTHTQDATPLTLGQEFSGYATQVKYGIERVTNTLPRIYQLAQGGTAVGTGLNTKKGFDIKIAAAVAEETQLPFLTAENKFEALAAHDAFVETSGAVNTVSVSLMKIANDIRFLGSGPRCGLGELLLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVAITVGGSNGHFELNVFKPLIASALLRSLTLLGDAAASFEKNCVRGIQANHRRIGQLVRESLMLVTALNPKIGYDKAAATAKKAHKEGSTLKEAALQLGVLTSEEFDELVVPEKMIGPSD

SNPs (tot: 7)

Position	A %	C %	G %	T %	Probability
764	90		9		0.999842
767			84	16	1.0
770			88	12	1.0
1038		88		11	0.923564
1595			91	8	1.0
1772			11	88	1.0
1906	10		89		0.999919

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain