SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2944

Length: 1713 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene2944

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Exostosin-2, putative (EC:2.4.1.223); K02367 glucuronyl/N-acetylglucosaminyl transferase EXT2 [EC:2.4.1.224 2.4.1.225]	-	-	68%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	87%
Blast2go	n-acetylglucosaminyltransferase-like protein	-	-	82%

Source	ECs	Term	Type	e value	Identity
AutoFact	Glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-	EC:2.4.1.224	-	0.0	68%

Source	KEGGs	Term	Type	Identity
AutoFact	Glycosaminoglycan biosynthesis - heparan sulfate	00534		68%
AutoFact	Metabolic pathways	01100		68%
AutoFact	N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase.	EC:2.4.1.225	-	68%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Glycosaminoglycan biosynthesis - heparan sulfate	00534		0.0	68%
AutoFact	Metabolic pathways	01100		0.0	68%

Source	GOs	Term	Type	Identity
Blast2go	cell-cell adhesion	GO:0016337	Biological Process	82%
Blast2go	phloem or xylem histogenesis	GO:0010087	Biological Process	82%
Blast2go	transferase activity, transferring glycosyl groups	GO:0016757	Molecular Function	82%
Blast2go	Golgi apparatus	GO:0005794	Cellular Component	82%

Source	InterPros	Term	Type	e value	Identity
Blast2go	EXTL2, alpha-1,4-N-acetylhexosaminyltransferase	IPR015338	-	0.0	82%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ADK4

Fln msg: Overlapping hits, possible frame ERROR between 524 and 523,

Fln protein: MRVHHRKRSLVKWIRQLLTNMVSFQRKLLLLFCILFALILIVGRAGSFMGWHRHSTELKSPASRGGYTVLINTWRQNSVLKQSVAHYASCSSVDAVRVVWSDTDSPSESLKAYLRKTVRSKAKSNHKPDFRFDLNEEDNLNNRFKPIEGLRTDAIxxSIDDDVVVPCSTLEFAFSVWQSAPDSMVGFVPRMHWVDTKNTDAIRYKYGGWWSVWWMGTYSMVLSKAAFFHQKYLEMYTYHMPASIQEYVSRERDCEDIAMSFLVANATGAPPVWVKGKLFEIGSGGISSLKGHSQRRNKCLNDFVSLYGYMPLVASNFNAVNAQYEWFW*

Protein Length: 330

Fln nts: CGTCGTGACCTTGGCTGTCACTCATTTTTGTTATATCCAGATAACAAGGTGCTGTC_-_ATGCGAGTTCATCACAGGAAACGATCCCTCGTGAAATGGATTCGGCAGCTGCTAACCAACATGGTCTCCTTTCAGAGAAAATTGCTCTTATTGTTCTGTATATTATTTGCGCTCATTTTAATTGTGGGCCGCGCTGGTTCCTTCATGGGATGGCACCGCCATTCCACTGAACTCAAATCCCCTGCCTCCAGGGGGGGGTATACAGTACTAATTAATACATGGAGGCAAAATTCAGTTCTCAAGCAATCGGTGGCACATTATGCATCTTGTAGCAGTGTTGATGCAGTACGTGTTGTGTGGAGTGATACTGATTCACCATCAGAATCTCTCAAGGCATATCTCAGGAAGACTGTGCGTTCGAAAGCAAAAAGTAATCACAAACCTGACTTTAGGTTTGACCTGAACGAGGAGGACAATCTCAACAACAGATTTAAACCAATCGAGGGCTTGAGAACAGATGCAATTxxxxxxTCTATTGATGATGATGTTGTAGTTCCATGTTCCACCCTGGAGTTTGCATTTAGTGTGTGGCAAAGCGCTCCAGATAGCATGGTTGGGTTTGTCCCACGCATGCATTGGGTTGATACAAAGAACACTGATGCTATACGTTATAAATATGGTGGCTGGTGGTCTGTTTGGTGGATGGGGACATATAGCATGGTGCTTTCCAAAGCAGCCTTTTTTCATCAAAAGTATTTGGAAATGTATACTTATCACATGCCAGCATCAATTCAAGAATATGTGAGCAGGGAAAGGGATTGCGAAGATATTGCAATGTCTTTCCTTGTTGCAAATGCAACTGGAGCTCCTCCTGTTTGGGTTAAAGGCAAATTATTTGAAATTGGTTCAGGCGGCATTAGTAGCTTGAAAGGACACAGTCAACGGAGAAATAAATGTCTCAATGACTTTGTTTCTCTATATGGTTATATGCCATTGGTTGCCAGTAATTTTAACGCTGTTAATGCTCAATATGAATGGTTTTGGTAG___CTCTGCTTCAATTTCTTATTGCATTCTTGTTATGATCAGATTTTGTGGGCATTTCGTTTATGCCAGGTATTGAAGTATATTACCTGCCTTGATAAAACATCACTGTTCTTTCTGTACCATTGGACTTGAACGAACTTTTAATTTTTCCCGGTTCTTTTGGTGTAGTCCAACCCTATTTTAAAGATAATCAAGTACTGTTACTTCTTGGAAGATGAATTTTGAAGGGTTCATTTGTTGTTACACCTTTTGTTTAGGTTGGAGACAACCTATGTATTTAATCATGTTGAGACTTCATGTTTTGGAAATTCAGTTTCACTCCATATTTTCAACGTCAGTTTGAATGGTGGGGACTCATCAAACTGTCTAATTATCTCAATACAAATGTATTTGTGCTAAATAGTGTAAAAAGGGTGGTTCCTGTTGTATGCCTGTATTGGTCTCTCATTTTGTTGTCAAAGTGGTTAATGAGAAGATGCACAGGGAGCATCTGCTTTGAGGATACGTTTGAACCGTTATGTGGCACAAGCAGGATATTGTACATATTACGGGCAATACCTATGACACCCTTTAAATGAAATGGATAATGATGGGATTGTAAGGAAAGTGCTTGATTTTCTATTCTTGAAGCGAAAGTTCTCGTACCTTAAATTGTATTGCAATAATCTTGTTATT

Fln Alignment:

sp_v1.1_unigene2944___MRVHHRKRSLVKWIRQLLTNMVSFQRKXXXXXXXXXXXXXXVGRAGSFMGWHRHSTELKSPASRGGYTVLINTWRQNSVLKQSVAHYASCSSVDAVRVVWSDTDSPSESLKAYLRKTVRSKAKSNHKPDFRFDLNEEDNLNNRFKPIEGLRTDAIxSIDDDVVVPCSTLEFAFSVWQSAPDSMVGFVPRMHWVDTKNTDAIRYKYGGWWSVWWMGTYSMVLSKAAFFHQKYLEMYTYHMPASIQEYVSRERDCEDIAMSFLVANATGAPPVWVKGKLFEIGSGGISSLKGHSQRRNKCLNDFVSLYGYMPLVASNFNAVNAQYEWFW
D5ADK4______________MRVHHRNRSLVKWIRQVLTNIGSLKTKLLLSFCMLFALSLIVGRAGSFMGWHRHSTELKFPSSRGGYTVLINTWRRNSVLKQSVAHYASCSSVDAIRVVWSENNPPSESLKAYLRKSVRSKAKSNHKPDFRFDLNEQDNLNNRFKPIEGLRTDAIxSIDDDVVVPCSTLEFAFSVWQSAPYSMVGFVPRMHWVDTKSADAIRYKYGGWWSVWWMGTYSMVLSKAAFFHQKYLEMYTYHMPASIQEYVSRERNCEDIAMSFLVANATGAPPVWVKGKLFEIGSSGISSLKGHSQRRNECLNDFVSLYGYMPLVASNFNAVNVQYEWFW

SNPs (tot: 48)

Position	A %	C %	G %	T %	Probability
91	80	20			0.995493
92	80		20		0.995493
94		20	80		0.995493
96	80			20	0.995493
97			20	80	0.995493
98	20			80	0.995493
100		20	80		0.995493
101			80	20	0.995493
102		80		20	0.995493
103	80		20		0.995493
110	80	20			0.995493
113		80		20	0.995493
114	80	20			0.995493
123			16	83	0.994512
124		83		16	0.994512
126			16	16	0.988813
128			16	83	0.994512
129	16			83	0.994512
131	83	16			0.994512
132			83	16	0.994512
133	83			16	0.994512
135	83		16		0.994512
136	83	16			0.994512
137	83			16	0.994512
138	83		16		0.994512
140		16		83	0.994512
141	16		83		0.994512
146	16			83	0.994512
149		16		83	0.994512
150		16	83		0.994512
151		16		83	0.994512
152		16		83	0.994512
330			85	14	0.993533
458		75	25		0.996473
459		75	25		0.996473
460			25	75	0.996473
529		25		75	0.996473
530		75		25	0.996473
652	88			11	0.923564
658		11	88		0.923564
661	88			11	0.923564
662			11	88	0.923564
663	88		11		0.923564
667		11		88	0.923564
707		90	10		0.90298
708	90		10		0.90298
709			10	90	0.90298
1168		42	57		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain