SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2724

Length: 2729 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene2724
CGTCGTGACCTTGGCTGTCACTCATCTAGGGTAGTCTTTTCAGAGGATCTTAAGAGCTCAGAGAGGAAGATATTTGATCCTCAAGACAAGTCTCTCCAGAGGTGGAATAGATTTTTTGTTGTCTCGTGCATAATTGCAGTGGCGTCGTGACCTTGGCTGTCACTCATATTTGCCAGTTGTTGATGATTTATTAAGATGCCTTGATATAGATTGGAACTTGGCAGTTACTGCGACTGTTCTTCGGACTCTTCTAGATACCTTTTATTTAGTTCACATGGCCTTCCAGTTTCGAACAGCCTTCATAGCTCCATCTTCTAGGGTTTTTGGAAGAGGAGAGCTTGTGATAGATCCTGTGCAAATTGCAAAGCGGTACTTTTTGACATCCTTCGCAATAGATATTCTTGCGGTCTTGCCTCTTCCTCAGGTTGTTATCTGGATATACATCCGAAGTAGGACAGGTTCAGCTGTCTTGAATGTGAAGAATTTTTTGAAGTTTATTGTCTTCTTTCAATATTTCCCAAGATTGTATCGTATATATCCACTTACGTTGGAGGTGGTAAAGACGGCCGGAGTTTTTACTGAAACGGCATGGGCTGGGGCTGCATACAATTTGATTCTATACATGCTTGCAAGTCATGTTGTTGGAGCTTGCTGGTATCTACTTTCAGTGGAGCGAGAAGATAGTTGTTGGCGAAAATCATGCCACAATAATTCTTCAGTGTGTAAAGTTGATTACTTGTACTGTGGGAATGACAATGATGATGGTTTCACCCACTGGAGTAATAATAGCTACAACATTTTGTATGTTACCGACTGTCCTGCGACTGACAGTGGAGATGGACCATTCAACTTTGGAATATATTCTAGTGCTCTTACTTCGCGGATTATTGCATCAAGAAAGTTTGTGCAGAAGTATTTTTACTGCTTATGGTGGGGTCTGCAAAACCTAAGCACACTTGGCCAGGGTCTGCAAACAACCAAGTACGTTGGAGAGGTGGTCTTTTCAATAACCCTTGCAGTCCTGGGGCTCATATTGTTTGCACTTCTCATCGGTAATATGCAGACCTATTTGCAATCTTTGACAATTAGGCTGGAAGAAATGAGAGTAAAGAGGCGTGATACAGAGCAATGGATGCGCCATCGTTTGCTTCCAGAGGAACTTAAGAAACGTGTTCGTCGGTATGACCAATATAAGTGGCTTGAAACAAGAGGAGTTGATGAAGAGAACTTAGTTCAGAGCCTTCCCAAGGACCTGAGACGTGACATCAAACGGCATCTCTGTCTGGGCTTGGTCAAACGGGTGCCTTTGTTTGGAAGCATGGATGACCGTTTGCTAGATGCAGTATGCGAGCGTCTGAAGCCATCCCTTTACACAGAAGATACTTACATTGTAAGAGAGGGAGATCCTGTTGATGAGATGCTTTTCATTATTCGGGGTCATTTAGATAGCGTGACAACAGGCGGAGGGAGAACTGGGTTTTTTAATCGTGGCGCTCTTAAACAAGGGGACTTTTGTGGGGAGGAACTACTCACATGGGCTTTGGATCCCAAATCAGGTGCAAACCTTCCCTCTTCTACTAGAACAGTGAGAGCTTTGTCCGAGGTAGAGGCATTTGCTTTGAGAGCAGAGGATCTGAAGTTTGTTGCAACCCAGTTCAGGCGTTTGCACAGCAAACAGATTCAACATACATTTCGTTATTACTCTCAACATTGGCAAACATGGGCTGCTTGTTTCATTCAAGCAGCTTGGCGCAGACACTGCAAACGGAAAAATGATGAATTCCGCCGTAGAGAGGAGCTAAAATTACGGACTGCTATGACAGCAAACAGTGGTGCTGCTTCTTCCAGTCTTGGAGCAACTATTTATGTATCCCGTTTTGCTACTAATGCTCTTCGTGGAGTTCAAAGATTGCGTAATACGAAGTTACCAGAACCAACGACACCACTACTTAAACTTCAAAAGCCATCAGAGCCCGACTTCAGCGCTGAAGATTCCTAATAGCCTCTTCATTTTGCATCGCACAAGGACGCAAGGATTTTTCTTCTAATAACAAAATGTTGGCTAGTCATTGACGAGCATTTGGAGAGAATGGAAATCTCCATCCTGTGTCTTTTCTGCCCAAATTTGGAGATGTGCTGGAAAAAGAAAATAGTTGGTATTTTATGTGAAGTCTCAAGTCAATATTCTGTACAGAATGACAGTCAGCATGCTTTCTCCTTATGATCAGGCGTTTAAGATCAATCTTTATGTATATTTCCTTTCAGCGCTGGCATGTTTTGGTCCTTGTAGATCCCTTGTGTTGTAAGTGTCAGAAATAAGCTTTTTCACGTCTCCCAGGATGTTTTGGTCTTCTTACCTATATAGCTGATTTAATCGTGTCTAATCAGCATAAAATGATCTTGAAAATTTCAGCACCACAAAGCAGATGCAAACATGGGAGTAAAAGCCCTTTCGTTCTTGGCTATAAGCTTTTTATGGCTTTATCTCATAGGTTGCTGATGACTGAGCAGTTTGGAGACCATTTGAATGTAGAAATATCTGAACTGGCACTAATACCTTTGCTTGATCGCGATAACATAAATTATAATTTCACCGAATTCATATGTTAATTATATTTCATCCATCTTTACATTTACACCTCCTCTTTGGCTCACTCGGCTTTTATCATTATCATCCTTCTTTTGGATGCACATAATTTCTGAAAAGATTTCTTTGAAATTTTACAGAG

Ace file of the UniGene sp_v1.1_unigene2724

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	similar to ATCNGC5 (CYCLIC NUCLEOTIDE GATED CHANNEL 5); calmodulin binding / cyclic nucleotide binding / potassium channel	-	-	62%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	27%
Blast2go	cyclic nucleotide and calmodulin-regulated ion channel	-	-	78%

Source	GOs	Term	Type	e value	Identity
Blast2go	calmodulin binding	GO:0005516	Molecular Function	0.0	78%
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	78%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LPV4

Fln msg: query STOP codon is far from subject stop. Distance to subject end: 21 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 575 - 659

Fln protein: MAFQFRTAFIAPSSRVFGRGELVIDPVQIAKRYFLTSFAIDILAVLPLPQVVIWIYIRSRTGSAVLNVKNFLKFIVFFQYFPRLYRIYPLTLEVVKTAGVFTETAWAGAAYNLILYMLASHVVGACWYLLSVEREDSCWRKSCHNNSSVCKVDYLYCGNDNDDGFTHWSNNSYNILYVTDCPATDSGDGPFNFGIYSSALTSRIIASRKFVQKYFYCLWWGLQNLSTLGQGLQTTKYVGEVVFSITLAVLGLILFALLIGNMQTYLQSLTIRLEEMRVKRRDTEQWMRHRLLPEELKKRVRRYDQYKWLETRGVDEENLVQSLPKDLRRDIKRHLCLGLVKRVPLFGSMDDRLLDAVCERLKPSLYTEDTYIVREGDPVDEMLFIIRGHLDSVTTGGGRTGFFNRGALKQGDFCGEELLTWALDPKSGANLPSSTRTVRALSEVEAFALRAEDLKFVATQFRRLHSKQIQHTFRYYSQHWQTWAACFIQAAWRRHCKRKNDEFRRREELKLRTAMTANSGAASSSLGATIYVSRFATNALRGVQRLRNTKLPEPTTPLLKLQKPSEPDFSAEDS*

Protein Length: 576

Fln nts: CGTCGTGACCTTGGCTGTCACTCATCTAGGGTAGTCTTTTCAGAGGATCTTAAGAGCTCAGAGAGGAAGATATTTGATCCTCAAGACAAGTCTCTCCAGAGGTGGAATAGATTTTTTGTTGTCTCGTGCATAATTGCAGTGGCGTCGTGACCTTGGCTGTCACTCATATTTGCCAGTTGTTGATGATTTATTAAGATGCCTTGATATAGATTGGAACTTGGCAGTTACTGCGACTGTTCTTCGGACTCTTCTAGATACCTTTTATTTAGTTCAC_-_ATGGCCTTCCAGTTTCGAACAGCCTTCATAGCTCCATCTTCTAGGGTTTTTGGAAGAGGAGAGCTTGTGATAGATCCTGTGCAAATTGCAAAGCGGTACTTTTTGACATCCTTCGCAATAGATATTCTTGCGGTCTTGCCTCTTCCTCAGGTTGTTATCTGGATATACATCCGAAGTAGGACAGGTTCAGCTGTCTTGAATGTGAAGAATTTTTTGAAGTTTATTGTCTTCTTTCAATATTTCCCAAGATTGTATCGTATATATCCACTTACGTTGGAGGTGGTAAAGACGGCCGGAGTTTTTACTGAAACGGCATGGGCTGGGGCTGCATACAATTTGATTCTATACATGCTTGCAAGTCATGTTGTTGGAGCTTGCTGGTATCTACTTTCAGTGGAGCGAGAAGATAGTTGTTGGCGAAAATCATGCCACAATAATTCTTCAGTGTGTAAAGTTGATTACTTGTACTGTGGGAATGACAATGATGATGGTTTCACCCACTGGAGTAATAATAGCTACAACATTTTGTATGTTACCGACTGTCCTGCGACTGACAGTGGAGATGGACCATTCAACTTTGGAATATATTCTAGTGCTCTTACTTCGCGGATTATTGCATCAAGAAAGTTTGTGCAGAAGTATTTTTACTGCTTATGGTGGGGTCTGCAAAACCTAAGCACACTTGGCCAGGGTCTGCAAACAACCAAGTACGTTGGAGAGGTGGTCTTTTCAATAACCCTTGCAGTCCTGGGGCTCATATTGTTTGCACTTCTCATCGGTAATATGCAGACCTATTTGCAATCTTTGACAATTAGGCTGGAAGAAATGAGAGTAAAGAGGCGTGATACAGAGCAATGGATGCGCCATCGTTTGCTTCCAGAGGAACTTAAGAAACGTGTTCGTCGGTATGACCAATATAAGTGGCTTGAAACAAGAGGAGTTGATGAAGAGAACTTAGTTCAGAGCCTTCCCAAGGACCTGAGACGTGACATCAAACGGCATCTCTGTCTGGGCTTGGTCAAACGGGTGCCTTTGTTTGGAAGCATGGATGACCGTTTGCTAGATGCAGTATGCGAGCGTCTGAAGCCATCCCTTTACACAGAAGATACTTACATTGTAAGAGAGGGAGATCCTGTTGATGAGATGCTTTTCATTATTCGGGGTCATTTAGATAGCGTGACAACAGGCGGAGGGAGAACTGGGTTTTTTAATCGTGGCGCTCTTAAACAAGGGGACTTTTGTGGGGAGGAACTACTCACATGGGCTTTGGATCCCAAATCAGGTGCAAACCTTCCCTCTTCTACTAGAACAGTGAGAGCTTTGTCCGAGGTAGAGGCATTTGCTTTGAGAGCAGAGGATCTGAAGTTTGTTGCAACCCAGTTCAGGCGTTTGCACAGCAAACAGATTCAACATACATTTCGTTATTACTCTCAACATTGGCAAACATGGGCTGCTTGTTTCATTCAAGCAGCTTGGCGCAGACACTGCAAACGGAAAAATGATGAATTCCGCCGTAGAGAGGAGCTAAAATTACGGACTGCTATGACAGCAAACAGTGGTGCTGCTTCTTCCAGTCTTGGAGCAACTATTTATGTATCCCGTTTTGCTACTAATGCTCTTCGTGGAGTTCAAAGATTGCGTAATACGAAGTTACCAGAACCAACGACACCACTACTTAAACTTCAAAAGCCATCAGAGCCCGACTTCAGCGCTGAAGATTCCTAATAGCCTCTTCATTTTGCATCGCACAAGGACGCAAGGATTTTTCTTCTAATAACAAAATGTTGGCTAGTCATTGACGAGCATTTGGAGAGAATGGAAATCTCCATCCTGTGTCTTTTCTGCCCAAATTTGGAGATGTGCTGGAAAAAGAAAATAGTTGGTATTTTATGTGAAGTCTCAAGTCAATATTCTGTACAGAATGACAGTCAGCATGCTTTCTCCTTATGATCAGGCGTTTAAGATCAATCTTTATGTATATTTCCTTTCAGCGCTGGCATGTTTTGGTCCTTGTAGATCCCTTGTGTTGTAAGTGTCAGAAATAAGCTTTTTCACGTCTCCCAGGATGTTTTGGTCTTCTTACCTATATAGCTGATTTAATCGTGTCTAATCAGCATAAAATGATCTTGAAAATTTCAGCACCACAAAGCAGATGCAAACATGGGAGTAAAAGCCCTTTCGTTCTTGGCTATAAGCTTTTTATGGCTTTATCTCATAGGTTGCTGATGACTGAGCAGTTTGGAGACCATTTGAATGTAGAAATATCTGAACTGGCACTAATACCTTTGCTTGATCGCGATAACATAAATTATAATTTCACCGAATTCATATGTTAATTATATTTCATCCATCTTTACATTTACACCTCCTCTTTGGCTCACTCGGCTTTTATCATTATCATCCTTCTTTTGGATGCACATAATTTCTGAAAAGATTTCTTTGAAATTTTACAGAG

Fln Alignment:

sp_v1.1_unigene2724___RKFVQKYFYCLWWGLQNLSTLGQGLQTTKYVGEVVFSITXXXXXXXXXXXXXXNMQTYLQSLTIRLEEMRVKRRDTEQWMRHRLLPEELKKRVRRYDQYKWLETRGVDEENLVQSLPKDLRRDIKRHLCLGLVKRVPLF-GSMDDRLLDAVCERLKPSLYTEDTYIVREGDPVDEMLFI
B8LPV4______________KSFWIRYISCIYWSITTLSTVGYGDIHAVNTREMIFIIFYVFFNLGLTAYLIGNMTNLVVQGTSRTMQFRNKIRAASNFGNRNDLPPKLKEQILSYMCLKF-RAEELQQQKVMEELPKSIRTSISRCLFIETVETVYLFQGVSTEFILDLVTEMHAEYFPPKEDILLQNETPSDIYLLV

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
1284		83		16	0.994512
1865	80		20		0.995493
2155	90			10	0.90298
2156	90		10		0.90298
2157			10	90	0.90298
2158	90		10		0.90298
2190	83	16			0.999991
2191			16	83	0.999991
2198		83	16		0.999991
2200		16	83		0.999991
2201	83	16			0.999991
2202	83			16	0.999991
2205	83		16		0.999991
2207	83			16	0.999991
2212			83	16	0.999991
2273	8		91		0.999807
2277	8	8	82		0.999136
2278	8	91			0.999807
2279	91	8			0.999807
2283			8	91	0.999807
2643	14	85			0.993533

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain