SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2689

Length: 1332 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene2689 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	GrpE protein homolog n=1 Tax=Picea sitchensis RepID=A9NPB5_PICSI	-	-	50%
FL-Next	sp=GrpE protein homolog; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	50%
Blast2go	protein	-	-	76%

Source	GOs	Term	Type	Identity
Blast2go	copper ion binding	GO:0005507	Molecular Function	76%
Blast2go	thylakoid	GO:0009579	Cellular Component	76%
Blast2go	chloroplast	GO:0009507	Cellular Component	76%

Source	InterPros	Term	Type	Identity
Blast2go	GrpE nucleotide exchange factor	IPR000740	-	76%
Blast2go	GrpE nucleotide exchange factor, head	IPR009012	-	76%
Blast2go	GrpE nucleotide exchange factor, coiled-coil	IPR013805	-	76%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NPB5

Fln msg: Separated hits, possible frame ERROR between 898 and 954,

Fln protein: MTAALASAALLLANTGVADIYVSRKFGISFKPRNSLKKTTEPFFHRVGCSFVTPADALPQRRQLPFAASDEVAETEPSELSEVENNDEEIEENDADTSDDADVEQEPPSLMQMLKQSYKQAIAVNDESKIAEVETQLQSMEDERNTLSQQALCLREELATEKERFLRLNADFDNFRKRSEKERLSLASNVQAEVIERLLPILDSFELAKTQIKIETEGEEKLNNSYQSIYKQISEVMRALGVSVVETIGKLFDPMLHEAVxxxxxxxxxxxxxxxxxxxxGFILGDRLLRPAMVKVSAGPGPVKPAEPSTEMVDPSEVTAEAPEETAETPEETTRITDDPLPSADASPAES*

Protein Length: 353

Fln nts: AGCAGTGGGTATCAACGCAGAGTACATGGGGACGGATGATTTTTCTACTCTATGAGGTAGAGGGGTTCAAATTCTCTCCGGCTACACGTTCGTAATATATTTCAACAACTACGCC_-_ATGACAGCTGCACTAGCAAGTGCAGCGCTTTTGTTAGCCAACACTGGTGTAGCAGATATCTATGTATCGCGGAAATTCGGAATTTCATTTAAACCACGGAATTCCTTGAAGAAAACGACCGAACCTTTCTTTCACAGGGTTGGATGCTCCTTCGTTACTCCCGCCGATGCACTCCCACAGCGACGCCAGTTGCCGTTCGCGGCGTCTGACGAAGTTGCAGAAACTGAACCTTCTGAGCTGTCAGAGGTGGAAAATAATGATGAGGAGATTGAGGAAAATGATGCTGATACCTCTGATGATGCAGATGTTGAACAAGAACCTCCGTCACTTATGCAAATGTTGAAACAGTCCTACAAGCAAGCCATTGCAGTTAATGATGAAAGTAAAATTGCAGAGGTAGAGACTCAGCTTCAGTCTATGGAAGATGAGAGGAATACTTTGTCACAACAAGCATTGTGTTTAAGAGAAGAGTTGGCAACTGAAAAGGAACGGTTCCTTCGTCTCAATGCAGACTTCGACAATTTCAGGAAAAGGTCAGAGAAAGAACGGCTATCTCTTGCATCTAATGTTCAGGCGGAAGTTATTGAGAGGCTCTTGCCCATTCTGGATAGTTTCGAACTAGCGAAAACACAGATCAAGATAGAAACAGAAGGAGAGGAAAAACTAAACAATAGCTACCAGAGTATATATAAACAGATTAGTGAAGTCATGAGAGCATTGGGTGTCTCTGTTGTAGAGACAATTGGCAAGCTATTTGATCCCATGCTCCATGAAGCAGTTATxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCGGCTTCATCCTTGGAGATAGGCTTTTGCGCCCCGCTATGGTGAAAGTTTCAGCTGGTCCAGGCCCTGTAAAGCCTGCTGAGCCTTCTACAGAAATGGTTGATCCATCAGAAGTGACAGCTGAAGCACCAGAAGAGACAGCTGAAACACCAGAAGAAACCACTCGAATCACTGATGATCCACTGCCATCGGCAGATGCCTCTCCGGCTGAATCTTAA___TCGAATTCATAGAATGGCTCTATTTCTCATAGTAAATTTCTGTAAATACGAGTTGAAACATGAGTTTCCAATATAGAATGGCCTGTTCTTATCTTCTCTCCACATTAAAAGTCATGAAATGGATGCGTCTGTAATATATATATATATATATATTAGAGTTTAT

Fln Alignment:

sp_v1.1_unigene2689___KTTEPFFHRVGCSFVTPADALPQRRQLPFAASDEVAETEPSXXXXXXXXXXXXXXXXXDTSDDADVEQEPPSLMQMLKQSYKQAIAVNDESKIAEVETQLQSMEDERNTLSQQALCLREELATEKERFLRLNADFDNFRKRSEKERLSLASNVQAEVIERLLPILDSFELAKTQIKIETEGEEKLNNSYQSIYKQISEVMRALGVSVVETIGKLFDPMLHEAVMxxxxxxxxxxxxxxxxxxxGFILGDRLLRPAMVKVSAGPGPVKPAEPSTEMVDPS
A9NPB5______________KSSGRFTNRATVGFVPLKPV--NRRRSTFVVYQKVAETESKQVEEESDDELEESDTED--SKAATAEQKPASLVPTLLQAYKEAIANSDDTRVSDIEVQLQAIEDEKNALSKLVAALTEEASRGKDKLLRLNADFDNFRKRAEKERLSLASNIQGDVIESLLPMVDDFERAKTQIKIQTEGEEKIDKSYQGIYKQFVEIMKGLHVNVVDTVGKPFDPMLHEAILxxxxxxxxxxxxxxxxxxxGFILGDKLLRPAMVKVSAGPGPAKDAEDSANDAEHS

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
647		75		25	0.996473
763	90	10			0.90298
764	90			10	0.90298
765	10	90			0.90298
1044		14		85	0.993533
1045	14		85		0.993533
1087		14		85	0.993533
1108	14			85	0.993533
1214	88	11			0.923564
1215		11	88		0.923564
1217		11		88	0.923564
1218	22		77		0.999999
1239	12			87	0.992554
1243	87	12			0.992554
1244		12		87	0.992554
1245		12	12	75	0.975346
1247		87		12	0.992554
1251		85	14		0.993533
1254		14	85		0.993533
1255	14			85	0.993533
1256	85	14			0.993533
1258	14	85			0.993533
1279		80		20	0.995493

SSRs (tot: 1)

Start position	End position	Sequence	Length
11	30	AT AT AT AT AT AT AT AT AT AT	20

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain