SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2425

Length: 2542 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene2425
GCTACTAGTGTTGTATCCTTGAATTCAGATGGGCTTTCTCTTTTGGCATTCAAAGCTGCAATAACTTCAGATCCAATGCATGCACTCCTGGACTGGAATGAGAATGATACAACGCCCTGCAGGTGGACAGGAGTCTCTTGCATGACGCTGGCGGGGAGCTCGGACCCTAGAGTTGTGGGCCTCACTGTAACAGGAAAATCTCTGGAAGGTTATATCCCTTCTGAGTTAGGAGCTCTGGTTTTTCTCCGTAGGTTGAACCTTCACAGCAATAATCTGAATGGCGCAATACCCACCCAGTTATTCAACGCTTCTGCGCTTCATAGTGTATTTCTTTACGAAAACAATCTCACGGGATCTATTCCTGTCGAGGTTACTAGATTGCCCAGACTTCAGAACCTGGATCTCTCGAGCAATTTCCTCAGTGGGTCTATTCCTCAAGAGTTGACGACGTGTAAACTGTTGCAGAGGTTGCTATTACAGAACAATTTCTTCACAGGGCCGATTCCAGCTGGGCTTGGATCAAACCTTAATAATCTAGTGCAGCTAGATCTCTCTAATAATCTTCTGAATGGCTCAATCCCTGGGGATCTCGGTAACCTTTCGTCATTGTCGGGCACTCTCAATCTTTCCTATAACAGGCTTTCGGGCCCAATTCCTCGAACTCTCGGTAAGTTGCCTTTGACTGTTAGTTTAGATTTACAGAACAATAATCTCAGTGGGCCGATTCCACAAGACGGATCTCTTGCAAATCAAGGACCAACCGCCTTTGTGAACAATTCAGGTCTTTGCGGATTTCCCTTAAAAAATCCTTGTAAAAAGTCTGCATCTACACCTCCTAATCTCAATTTTCCTTTACAAGGCCCTTCCTCAGGGGGCCCTACTAATCAAGGGACGAGCCCTACAGTAAGAAAAGGTCTCAAAACTGGTGTAATTGTTGCAATATCGCTGGGAGATGCTGTGGGTATTACCATTATTGGCCTACTGTTTGTCTATTTGTATTGGAAAACTAGGATCTGCCAATCCAAATGGTGCAGCTGTGCAGGAAAAGAGAAGTTGGCAGGAGGAGGGATTACTCAATGTTTTTGTAACAACACGGAGGCTTCTGAAACGGCCTCAGAGAAGGGAGACCAGGGAGATCTCATTCCCATCGACAAAGGTTTCAGCTTTGACTTAGATGAATTGCTCAGAGCTTCTGCTTATGTCTTGGGGAAGAGTGGATTGGGAATTGTGTACAAAGTGGTACTTGGCAATGGGATTCCAGTGGCAGTGAGGAGGTTGGGGGAGGGAGGAGTTCAGAGATTTAAGGAATTTGAGGCTGAAGTTCAAGCCATTGGACGAATTCGACATCCGAACATAGTGAAACTTCGAGCATATTATTGGGCAGCTGATGAGAAGTTGTTGATATCCGATTTCATTCCAAATGGAAGCTTGGCAGTTGCATTGCATGGTAGATCAGGCCATCCAGCTACACCTCTGCCATGGGCGTCTAGGATAAAAATTGCCAAAGGAACTGCACGCGGTCTGGCCTATCTGCATGAGTGCAGTCCTCGTAAATATGTACACGGAGACATTAAGCCCACAAATATTCTGTTGGATAACAATATGCAGCCATATATCTCAGATTTTGGCTTGGGTCGTCTCATTAGCATTGCAGGCAGTGGAGTTGCACCTCAACAAGGTGGAGGACTCCTTGGTGGAGCCTTTCCATCAGGCAAAACCTCAGTTGCTGCTCGTGGAAGCACATTCCCAGGCGGAGTTGTAAGATCGGCTGTAGAATGGCCAACTAATGGTGGAAGTTCACATTCATTCGTGAGTAATTACCAGGCTCCAGAAGCCTCTGTTGCTGGCCATAAATCAACACAGAAATGGGATGTTTATTCATTTGGAGTTGTCCTTTTGGAAATGCTTACAGGTCGATCTCCAGCATTCCAACTTGCATCCTTTGACATGGACTTGGTCACTTGGGTTAGAAAGGCATTCGAAGAGGAACACCCACTGTCAGAAATTGTGGATCCATCACTCCTGCAAGAAGTATATGCCAAGAAAGAAGTACTTGCTGTATTCCATATTGCTCTTGCATGTGTAGAAGCAGATCCTGATGTGCGACCCAGGATGAAGAATATATCTGATTCTCTTGACGCAATTGGAGCTACATCACAAAATTGAGAAGGCTACCAAGCTATGCAACTTGATCATCTGTTTACTCTGTTTTATGGGAGCTATTGCTCCCAACTTTTGACTCGGTATTCTGCTGAGAGTCATATCCAAGTGCAGAATCCATGACACGCAACACATTCTCTCCATCTGCTTTATTCCTGTATCTATAGGAAGCAATCTTCTGTTTCCTGTCGAGACCATTCAAGACCATTCATTGTTAAATTGAGGGTGCAGTTTAGTCCCCATTTTGATTAAAGTATCCTTCTGCTAGCATTTATTTATTTATAATGCAATTTTCAACTATTGTAAAGCAGCAATGCCTCAATTTTCACTTTAATGCATATGTTTCATAATTTCATTTCATTTTGTATTAAAATTAGAATAC

Ace file of the UniGene sp_v1.1_unigene2425 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	leucine-rich repeat family protein	-	-	53%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	39%
Blast2go	protein	-	-	66%

Source	GOs	Term	Type	e value	Identity
Blast2go	catalytic activity	GO:0003824	Molecular Function	0.0	66%
Blast2go	plasma membrane	GO:0005886	Cellular Component	0.0	66%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LN40

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 34 aas, putative chimeric sequence, No stop codon before M and low homology subject, your sequence is longer than subject: 697 - 340

Fln protein: MHALLDWNENDTTPCRWTGVSCMTLAGSSDPRVVGLTVTGKSLEGYIPSELGALVFLRRLNLHSNNLNGAIPTQLFNASALHSVFLYENNLTGSIPVEVTRLPRLQNLDLSSNFLSGSIPQELTTCKLLQRLLLQNNFFTGPIPAGLGSNLNNLVQLDLSNNLLNGSIPGDLGNLSSLSGTLNLSYNRLSGPIPRTLGKLPLTVSLDLQNNNLSGPIPQDGSLANQGPTAFVNNSGLCGFPLKNPCKKSASTPPNLNFPLQGPSSGGPTNQGTSPTVRKGLKTGVIVAISLGDAVGITIIGLLFVYLYWKTRICQSKWCSCAGKEKLAGGGITQCFCNNTEASETASEKGDQGDLIPIDKGFSFDLDELLRASAYVLGKSGLGIVYKVVLGNGIPVAVRRLGEGGVQRFKEFEAEVQAIGRIRHPNIVKLRAYYWAADEKLLISDFIPNGSLAVALHGRSGHPATPLPWASRIKIAKGTARGLAYLHECSPRKYVHGDIKPTNILLDNNMQPYISDFGLGRLISIAGSGVAPQQGGGLLGGAFPSGKTSVAARGSTFPGGVVRSAVEWPTNGGSSHSFVSNYQAPEASVAGHKSTQKWDVYSFGVVLLEMLTGRSPAFQLASFDMDLVTWVRKAFEEEHPLSEIVDPSLLQEVYAKKEVLAVFHIALACVEADPDVRPRMKNISDSLDAIGATSQN*

Protein Length: 698

Fln nts: GCTACTAGTGTTGTATCCTTGAATTCAGATGGGCTTTCTCTTTTGGCATTCAAAGCTGCAATAACTTCAGATCCA_-_ATGCATGCACTCCTGGACTGGAATGAGAATGATACAACGCCCTGCAGGTGGACAGGAGTCTCTTGCATGACGCTGGCGGGGAGCTCGGACCCTAGAGTTGTGGGCCTCACTGTAACAGGAAAATCTCTGGAAGGTTATATCCCTTCTGAGTTAGGAGCTCTGGTTTTTCTCCGTAGGTTGAACCTTCACAGCAATAATCTGAATGGCGCAATACCCACCCAGTTATTCAACGCTTCTGCGCTTCATAGTGTATTTCTTTACGAAAACAATCTCACGGGATCTATTCCTGTCGAGGTTACTAGATTGCCCAGACTTCAGAACCTGGATCTCTCGAGCAATTTCCTCAGTGGGTCTATTCCTCAAGAGTTGACGACGTGTAAACTGTTGCAGAGGTTGCTATTACAGAACAATTTCTTCACAGGGCCGATTCCAGCTGGGCTTGGATCAAACCTTAATAATCTAGTGCAGCTAGATCTCTCTAATAATCTTCTGAATGGCTCAATCCCTGGGGATCTCGGTAACCTTTCGTCATTGTCGGGCACTCTCAATCTTTCCTATAACAGGCTTTCGGGCCCAATTCCTCGAACTCTCGGTAAGTTGCCTTTGACTGTTAGTTTAGATTTACAGAACAATAATCTCAGTGGGCCGATTCCACAAGACGGATCTCTTGCAAATCAAGGACCAACCGCCTTTGTGAACAATTCAGGTCTTTGCGGATTTCCCTTAAAAAATCCTTGTAAAAAGTCTGCATCTACACCTCCTAATCTCAATTTTCCTTTACAAGGCCCTTCCTCAGGGGGCCCTACTAATCAAGGGACGAGCCCTACAGTAAGAAAAGGTCTCAAAACTGGTGTAATTGTTGCAATATCGCTGGGAGATGCTGTGGGTATTACCATTATTGGCCTACTGTTTGTCTATTTGTATTGGAAAACTAGGATCTGCCAATCCAAATGGTGCAGCTGTGCAGGAAAAGAGAAGTTGGCAGGAGGAGGGATTACTCAATGTTTTTGTAACAACACGGAGGCTTCTGAAACGGCCTCAGAGAAGGGAGACCAGGGAGATCTCATTCCCATCGACAAAGGTTTCAGCTTTGACTTAGATGAATTGCTCAGAGCTTCTGCTTATGTCTTGGGGAAGAGTGGATTGGGAATTGTGTACAAAGTGGTACTTGGCAATGGGATTCCAGTGGCAGTGAGGAGGTTGGGGGAGGGAGGAGTTCAGAGATTTAAGGAATTTGAGGCTGAAGTTCAAGCCATTGGACGAATTCGACATCCGAACATAGTGAAACTTCGAGCATATTATTGGGCAGCTGATGAGAAGTTGTTGATATCCGATTTCATTCCAAATGGAAGCTTGGCAGTTGCATTGCATGGTAGATCAGGCCATCCAGCTACACCTCTGCCATGGGCGTCTAGGATAAAAATTGCCAAAGGAACTGCACGCGGTCTGGCCTATCTGCATGAGTGCAGTCCTCGTAAATATGTACACGGAGACATTAAGCCCACAAATATTCTGTTGGATAACAATATGCAGCCATATATCTCAGATTTTGGCTTGGGTCGTCTCATTAGCATTGCAGGCAGTGGAGTTGCACCTCAACAAGGTGGAGGACTCCTTGGTGGAGCCTTTCCATCAGGCAAAACCTCAGTTGCTGCTCGTGGAAGCACATTCCCAGGCGGAGTTGTAAGATCGGCTGTAGAATGGCCAACTAATGGTGGAAGTTCACATTCATTCGTGAGTAATTACCAGGCTCCAGAAGCCTCTGTTGCTGGCCATAAATCAACACAGAAATGGGATGTTTATTCATTTGGAGTTGTCCTTTTGGAAATGCTTACAGGTCGATCTCCAGCATTCCAACTTGCATCCTTTGACATGGACTTGGTCACTTGGGTTAGAAAGGCATTCGAAGAGGAACACCCACTGTCAGAAATTGTGGATCCATCACTCCTGCAAGAAGTATATGCCAAGAAAGAAGTACTTGCTGTATTCCATATTGCTCTTGCATGTGTAGAAGCAGATCCTGATGTGCGACCCAGGATGAAGAATATATCTGATTCTCTTGACGCAATTGGAGCTACATCACAAAATTGAGAAGGCTACCAAGCTATGCAACTTGATCATCTGTTTACTCTGTTTTATGGGAGCTATTGCTCCCAACTTTTGACTCGGTATTCTGCTGAGAGTCATATCCAAGTGCAGAATCCATGACACGCAACACATTCTCTCCATCTGCTTTATTCCTGTATCTATAGGAAGCAATCTTCTGTTTCCTGTCGAGACCATTCAAGACCATTCATTGTTAAATTGAGGGTGCAGTTTAGTCCCCATTTTGATTAAAGTATCCTTCTGCTAGCATTTATTTATTTATAATGCAATTTTCAACTATTGTAAAGCAGCAATGCCTCAATTTTCACTTTAATGCATATGTTTCATAATTTCATTTCATTTTGTATTAAAATTAGAATAC

Fln Alignment:

sp_v1.1_unigene2425___FSFDLDELLRASAYVLGKSGLGIVYKVVLGNGIPVAVRRLGEGGVQRFKEFEAEVQAIGRIRHPNIVKLRAYYWAADEKLLISDFIPNGSLAVALHGRSGHPATPLPWASRIKIAKGTARGLAYLHECSPRKYVHGDIKPTNILLDNNMQPYISDFGLGRLISIAGSGVAPXXXXXXXXGAFPSGKTSVAARGSTFPGGVVRSAVEWPTNGGSSHSFVSNYQAPEASVAGHKSTQKWDVYSFGVVLLEMLTGRSP-AFQLASFDMDLVTWVRKAFEEEHPLSEIVDPSLLQEVYAKKEVLAVFHIALACVEADPDVRPRMKNI
B8LN40______________YTFDLEDLLRASAEVLGKGSVGTAYKAVLEDGTTVVVKRLKDVAANR-KDFEQQMELVGRIRHRNLVPLRAFYYSKDEKLLVYDYMPTGSLSALLHGSRGSGRTPLDWDTRMRIALGAARGISHIHEEGGGKFTHGNIKSSNVLLTTDLDGCVSDFGLVPLFSAAAAA-----------------------------------------------NRIAGYRAPEV-IETRKVTQKSDVYSFGVLLLELLTGKAPNQASLNDEGIDLPRWVQSVVREEW-TAEVFDVELMRYQNIEEEMVQLLQIAMACVAAVPDQRPRMQDV

SNPs (tot: 15)

Position	A %	C %	G %	T %	Probability
1094	33			66	0.997452
1437		75		25	0.996473
1479		80		20	0.995493
1497		80		20	0.995493
1705	75		25		0.996473
1785		40	60		1.0
1854		20		80	0.995493
1863		80	20		0.995493
1867		80	20		0.995493
1869	80	20			0.995493
1872		20	80		0.995493
1876		83	16		0.994512
1877			16	83	0.994512
1879	83	16			0.994512
1880	16	83			0.994512

SSRs (tot: 1)

Start position	End position	Sequence	Length
100	113	ATAA ATAA ATAA AT	14

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain