SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2409

Length: 1049 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene2409

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Isoamylase 3, chloroplastic n=2 Tax=Arabidopsis RepID=ISOA3_ARATH	-	-	65%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	31%
Blast2go	isoamylase isoform 3	-	-	79%

Source	ECs	Term	Type	e value	Identity
Blast2go	Isoamylase.	EC:3.2.1.68	-	0.0	79%

Source	GOs	Term	Type	Identity
Blast2go	starch catabolic process	GO:0005983	Biological Process	79%
Blast2go	isoamylase activity	GO:0019156	Molecular Function	79%
Blast2go	chloroplast starch grain	GO:0009569	Cellular Component	79%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ABA9

Fln msg: No stop codon before M and low homology subject, your sequence is longer than subject: 213 - 175

Fln protein: MNGESGRDGCNDNYSWNCGFEGETRDPAINALRSRQMKNFHLALMVSQGTPMILMGDEYAHTRYGNNNSYGHDTAMNHFQWNQLDARKESHFRFFAETIRFHRQHPLLGRETFLTKADITWHEKNWDNFDSRFLAFSLHEGKYGGGDLYIAFNAHDYFVKSVVPPPPSGKCWYRVVDTNLPSPDDFVVGGVPGVKKVYEMAPYSGVVLHAKP*

Protein Length: 214

Fln nts: CGTCGTGACCTTGGCTGTCACGCTACTTTTGCTACACGTATAGCAGGGTCTTCTGATTTGTATCAGATAAATGCACGCAAGCCATATCACAGCATCAATTTTGTTATTGCACATGATGGATTTACTTTATATGATCTTGTTGCTTATAACTCGAAGCACAATGAA_-_ATGAATGGAGAAAGTGGAAGGGATGGATGCAATGATAATTACAGCTGGAATTGTGGTTTTGAAGGTGAGACAAGGGATCCTGCCATCAATGCTCTCCGTTCACGTCAGATGAAAAATTTCCATTTAGCATTAATGGTTTCACAGGGAACACCTATGATTTTAATGGGAGATGAGTATGCTCACACTCGATATGGAAATAATAACAGTTATGGTCATGATACTGCCATGAACCATTTCCAGTGGAATCAGCTAGATGCAAGAAAGGAGAGTCACTTCAGGTTTTTTGCTGAGACTATTAGATTTCACCGACAACATCCTCTTTTGGGGCGTGAGACATTTCTAACCAAGGCTGATATCACTTGGCACGAGAAGAATTGGGATAATTTTGATAGCAGGTTTCTTGCATTTTCATTGCATGAAGGAAAATACGGAGGTGGAGATCTTTACATAGCTTTCAATGCACATGATTATTTTGTAAAATCTGTCGTGCCTCCACCACCCTCAGGAAAATGCTGGTATCGAGTGGTGGATACCAATCTTCCATCTCCAGATGACTTCGTTGTTGGAGGAGTTCCAGGCGTCAAAAAAGTTTATGAGATGGCTCCATATTCTGGAGTTGTCCTTCATGCAAAACCCTAAACTGAAATTCTAAAATATTACCAGTGCAAATCTTTGCAATCACTGCATTACAATTATTTTGGCCATATTCCCATGTGCTTAAAGCTTATTTAAGGTTCTACACAGACATATTTAGTCTGGCAAATGCCAATGGTATTGATCATGTCGAAGTGTCTATCTGAGAGCCTATTGCATGAACGTATGGACATGTGCCCAGATTTTCCCTTATTATTATATGAATTTCTAAACTTATTCAATAGTTTGAA

Fln Alignment:

sp_v1.1_unigene2409___MGDEYAHTRYGNNNSYGHDTAMNHFQWNQLDARKESHFRFFAETIRFHR--------QHPLLGRETFLTKADITWH-----EKNWDNFDSRFLAFSL------HEGKYGGGDLYIAFNAHDYFVKXXXXXXXXGKCWYRVVDTNLPSPDDF-----XXXXXXXXXXXYEMAPYSGVVLHAK
D5ABA9______________MGDEYGQTKGGSMLLNDRKP----FNWDALKTE------FAVETVRFIRFLSSFRTYRRDLLQRKTFAKIENLNWHGPIPDQPQWEDPNSSFLAISLKPERNENESRSTVGDLYIAFNSQKASVSATLPQLPDGMLWHRLVDTSLPFPKTFSMEGMATDMLGISFSTYQIQPHSCVLFEAR

SNPs (tot: 28)

Position	A %	C %	G %	T %	Probability
491		11		88	0.923564
494		11	88		0.923564
495	11		88		0.923564
497		88		11	0.923564
570	10			90	0.90298
573	10	90			0.90298
575			10	90	0.90298
576			10	90	0.90298
577	10			90	0.90298
578	10			90	0.90298
579	10	90			0.90298
581		10		90	0.90298
582	10			90	0.90298
584		90	10		0.90298
586			10	90	0.90298
588	90			10	0.90298
589	90		10		0.90298
596		33		66	1.0
681	20		80		0.995493
1015	66			33	0.997452
1016		33		66	0.997452
1017			33	66	0.997452
1020			33	66	0.997452
1022	66		33		0.997452
1023	33			66	0.997452
1024	66		33		0.997452
1031			33	66	0.997452
1032		33		66	0.997452

Back

Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain