SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2254

Length: 1036 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene2254 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	dolichyl-P-Man Man; K03847 alpha-1,6-mannosyltransferase [EC:2.4.1.130]	-	-	51%
FL-Next	Isoform 4 of Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase OS=Arabidopsis thaliana GN=ALG12	-	-	62%
Blast2go	transferring glycosyl groups	-	-	63%

Source	ECs	Term	Type	e value	Identity
AutoFact		EC:2.4.1.13-	-	0.0	51%

Source	GOs	Term	Type	Identity
Blast2go	ER-associated protein catabolic process	GO:0030433	Biological Process	63%
Blast2go	protein amino acid terminal N-glycosylation	GO:0006496	Biological Process	63%
Blast2go	alpha-1,6-mannosyltransferase activity	GO:0000009	Molecular Function	63%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: sp_plants

Fln subject: A8MR93-4

Fln msg: Overlapping hits, possible frame ERROR between 194 and 142, Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 249 - 449

Fln protein: S*PWLSLSVFPVHWYFTSALPRSMLAAFPLCFLGVILERRIREYMLxxxxxxxxxxxxxxxxxxxFIILAIPLLNLSAAIAATRIYNNRKKAIWKWAYIGMICSLFASLGFSAIMSAASYANYPAGYALDVLHHKDFNRTSSCVVHIDSFAAMNGISRFCENDFLWRYSKEEDLSVEEIIAKNFTYLINERSRIPEFKCLLAISGFSRLVVQLSFPPVRLLTEPKVFVHGNIRSEGIEDIRWPGCPYS*

Protein Length: 250

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGTGTATTTCCAGTGCATTGGTATTTTACGTCAGCTCTTCCACGATCAATGTTGGCGGCATTCCCACTATGCTTTCTAGGTGTAATCCTTGAGAGAAGGATACGAGAGTACATGTTAxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTTATAATTTTGGCAATTCCCTTGCTCAACCTCTCTGCAGCGATTGCAGCTACAAGAATTTACAACAATAGGAAGAAAGCAATCTGGAAGTGGGCATACATTGGCATGATATGCTCACTCTTTGCAAGTTTAGGGTTCTCTGCAATAATGAGTGCTGCATCATATGCAAACTATCCAGCTGGCTATGCACTAGATGTTCTGCATCATAAAGATTTTAACAGAACTTCAAGCTGTGTGGTTCACATTGATTCATTTGCAGCAATGAATGGAATTTCTCGTTTTTGTGAAAATGATTTTCTTTGGAGGTATTCTAAGGAAGAAGATCTTTCTGTTGAAGAAATCATTGCCAAAAATTTCACATATCTTATCAATGAACGGTCAAGAATACCCGAGTTTAAATGCTTATTGGCTATCTCTGGATTTTCTAGGCTAGTTGTTCAGCTCTCCTTCCCACCTGTTCGCCTGTTAACTGAGCCCAAAGTTTTTGTCCATGGAAATATAAGAAGTGAAGGTATTGAAGATATAAGATGGCCTGGTTGTCCTTATTCATAGTAGGAGAACCACACGATTGTCAACTTGTCTGATAATATTACTATAGTTGAAGTGTTTAATCTTAACTTTTCAAGAACGATATTTTTCCAGCAGCGCAGTGAAAGATTGCAATTATTGAATCATATTTAAAGGCTACTCTTTTGTAGTGGGAGTCTTTGTCATGCTTAGCTTAGAATTTTTATTGTAATAACATTTAATTTATTGGGTCGATAGATACTCTACAGTCCAGTATACGAGGGAGATGTTGTCACATTAATTTAATTTTTTAAATATGTGTTCAATTAAACTC

Fln Alignment:

sp_v1.1_unigene2254___VHWYFTSALPRSMLAAFPLCFLGVILERRIREYMLPVxxxxxxxxxxxxxxxxSSGFIILAIPLLNLSAAIAATRIYNNRKKAIWKWAYIGMICSLFASLGFSAIMSAASYANYPAGYALDVLHH--KDFNRTSSCVVHIDSFAAMNGISRFCENDFLWRYSKEEDLSVEEIIAKNFTYLINERSRIPEFKCLLAISGFSRLVVQLSFPPVRLLTEPKVFVHGNIRSEGIEDIRWPGC
A8MR93-4____________IHWYFTSALPRSLLVAYPLSLLGTLVDRRVPFFIVPVxxxxxxxxxxxxxxxx---FIISSVPMFNLSAAVAASRIYNNRKKTIWKLVNMVMLAFFAISAGCTVVTFMASYYNYPSGYALKRLHQIGHPANVAGEEWVHIDTFGAMNGISRFCEDDFPWRYSKEEEIVVEELRNRNFTYLVNEHSSVDGYKCLFYEEGFERLELRRGFPPIVLVKKAKVYLHREMKKEDPFHKKWPGC

SNPs (tot: 56)

Position	A %	C %	G %	T %	Probability
56	20		80		0.995493
105	80		20		0.995493
296	88		11		0.923564
315		70		30	1.0
351	11			88	0.923564
354	88			11	0.923564
358	88	11			0.923564
359	88		11		0.923564
362			11	88	0.923564
407	83		16		0.999991
410			16	83	0.999991
413	8	75	16		0.99999
414	16	83			0.999991
415		16		75	0.99999
419	72	27			1.0
422	72		27		1.0
423			27	72	1.0
424		58		33	1.0
425		83	16		0.999991
428	83			16	0.999991
432		22		77	0.999999
442	77	22			0.999999
444		20	80		0.999996
445		80	20		0.999996
447		20		80	0.999996
448			20	80	0.999996
589		16	83		0.994512
592	83			16	0.994512
607	60		40		1.0
737	83		16		0.994512
738	16			83	0.994512
747			16	83	0.994512
751		16		83	0.994512
753	16	83			0.994512
755		83	16		0.994512
854		33	66		1.0
855	33	66			1.0
856			33	66	1.0
860	33			66	1.0
861			33	66	1.0
864		33	66		1.0
865	33			66	1.0
866		33	66		1.0
869	66	33			1.0
884	75			25	0.996473
885		75	25		0.996473
887	75		25		0.996473
889	75		25		0.996473
891	75	25			0.996473
898	75		25		0.996473
900	75			25	0.996473
903		25	75		0.996473
909	75		25		0.996473
1021		33		66	0.997452
1022		33	66		0.997452
1023	66	33			0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain