SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2237

Length: 1068 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene2237 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	Polcalcin Jun o, putative (EC:1.3.1.74); K13448 calcium-binding protein CML	-	-	1.0e-34	54%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	50%
Blast2go	calmodulin-related protein	-	-	0.0	71%

Source	GOs	Term	Type	Identity
Blast2go	response to cold	GO:0009409	Biological Process	71%
Blast2go	regulation of flower development	GO:0009909	Biological Process	71%
Blast2go	cytoplasm	GO:0005737	Cellular Component	71%
Blast2go	nucleus	GO:0005634	Cellular Component	71%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNH6

Fln msg: Overlapping hits, possible frame ERROR between 398 and 317,

Fln protein: MGCPSFLHRKKTPADTPRRQSQMSNxxxxxxxxxxxxxxxxxxxxxxxxxxxxxLKLSLSRRSDGVSDKKVDKLSVGVGAGTSGSAVPSNADLEDVFKKFDANGDGKISPSELRDLMRSLGGNATDEEVGAMVLEADLNRDGFIDLSEFVALNTDHTVSSSRRIQDLQDAFNMFDKDRNGLISPNELHEVLKSLQEPCTLSDCQNMIKGVDSNGDGQVNFDEFKAMMTNPSDNSWTTVP*

Protein Length: 241

Fln nts: ATGGGGATTGTGGGTCAGCCGCTTTAGGAATTCAAAGTTGCAGAGAAGATATAGCTTCCGCATCAAGAATTATATGCTTGCTTTTTCTTATATTAAAGGGGATACTGGCAAACGAGGAAATTCAATTATCACGGAGCTGAGCTTTATTGGGACATTGAAGCGTTGTAATTGTGCAGAGATCATTGTAATTGTGCAGAGATCCGGGAAGGATAGAAATTTTCAATCTGGCGAGTATTAAA_-_ATGGGTTGCCCGTCGTTTCTTCACCGGAAGAAAACACCGGCCGACACACCCCGTCGACAATCACAAATGTCGAATxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxCTGAAACTGTCGCTCAGCCGAAGAAGCGATGGCGTGTCGGACAAGAAAGTCGATAAGCTTTCCGTCGGTGTCGGTGCGGGCACGTCCGGTTCTGCGGTGCCGAGTAATGCAGATCTGGAGGACGTGTTCAAGAAATTCGACGCCAACGGCGACGGGAAGATTTCTCCGTCGGAGCTGAGAGATTTGATGAGGTCGTTGGGTGGGAATGCGACGGATGAAGAGGTTGGGGCTATGGTTTTGGAGGCAGATTTGAACAGAGACGGTTTCATCGACCTGTCGGAGTTTGTGGCTCTCAACACCGACCACACAGTAAGCAGTTCGCGCAGAATCCAGGACTTGCAGGATGCATTCAATATGTTCGACAAGGACCGGAATGGATTAATCTCTCCCAATGAACTTCATGAAGTTTTGAAGAGCCTGCAAGAGCCTTGCACTCTCAGTGACTGCCAGAATATGATCAAAGGCGTCGATTCAAATGGCGATGGGCAGGTCAACTTTGATGAATTCAAGGCCATGATGACCAACCCATCTGATAATTCGTGGACTACCGTGCCTTAA___TTGCTTCGACAGCTAGCTTTAAGTGTTCTCTCTCGCATTGATATGATATTCCTTTATATGTTTCGACTCTCAATATCTCTCGCATGTATATATTCCTTTATATGTTTCAG

Fln Alignment:

sp_v1.1_unigene2237___MGCPSFLHRKKTPADTPRRQSQMSNIFxxxxxxxxxxxxxxxxxxxxxxxxxxXXXXXXXXXXSDGVSDKKVDKLXXXXXXXXXXXXXXSN----ADLEDVFKKFDANGDGKISPSELRDLMRSLGGNATDEEVGAMVLEADLNRDGFIDLSEFVALNTDHTVSSSRRIQDLQDAFNMFDKDRNGLISPNELHEVLKSLQEPCTLSDCQNMIKGVDSNGDGQVNFDEFKAMMTNPSDNSWTT
B8LNH6______________MGCPSLLHRKKTPADTSRRQSAMSNMFxxxxxxxxxxxxxxxxxxxxxxxxxxRRRKLSLSGRNDVVSDGKIDELGVGVGVDVGVDASRSAVPSAAELEDVFKKFDANGDGRISRSELSDLMKSLGGNVTEEEVGAMVSEADLDGDGYIDLSSFVALNTDQTVSSSRRVQDLKDAFNMFDRDGNGSISPSELHHVLTSLQEHCTIGDCHNMIKDVDSNGDGQVSFDEFMAMMTNTSHNSWTT

SNPs (tot: 5)

Position	A %	C %	G %	Probability
778	90		10	0.90298
891	90		10	0.90298
948		12	87	0.992554
949	87	12		0.992554
989	80		20	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain