SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2208

Length: 3484 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene2208
ATGGGGGGATTCCACGTTCCTCTACTTCCACATTCCCCAAAGGGTGGGTGTAATTGATACAAATATTTGCTTTGAGCACCATCCACTCATTTGCCCCCAACAACCTAATCTTCCCCTTTAAACCCTACCAAAACCCTACATATGCGCACGAAGGAAAATATCTTCGAACTCTTCCTCCCCCTCAACCAAAACCCTGCATATCCCGACGAAGGAAAATATCTTCGAACTCTTCCTCCCCCTCAAGTCTCCACGTTGTCCGCAGATCGACAGCTTGGGGAAGCCCTGCACTGTTGGGTTGGAGAGTGAGTTTGAAGTTAGCTACTAATCCACAATGGCGGGACGTCTTGGAGGAGCGCTTTTGCTGTACCCTACAGTTTCAACCATCAACAACATTTCGTGTAAAAATCTCAATCCTTGCTTCGCCTCTCAAGCATTCACGCTTTCTCCGAGTGCTTTTTCTTTAAATTTCCATATGTTTAGTATTGTAAGGAACGCCAAATTTACCCTGAGGCATTTCAATCCAAGTTCCAGAAAGAATAATTCAGTTGTTTTGGGTACCAGAAATCACTCAAGCCGCTCGTTTCGTTCTGAACGGGGTCGGGACGACGAAACTATCCCAGCTTGGCGAAGAACCCCTAAAACAACGACTTTTAACGCAAAAAGTACAAATAGACCAGAATCCAAAAGTCTGGTTGAAGAGGAACTGGATATGGATAACTTGTTTGGAAAACGGGGTGCATCTATGTCTGGTCGGTCGAATCCTGGTAGAAATGATTATAATGATGGATCGTCGAGGAATTATAGAGGCAATGATGTCTCTGCAAGGGGCAGACAAGGAGGGAAAGGCTCTTTTAATGGGGTCTCTCCAAGGGGCAGGCGAGGAGGGAATAGCTCGTTTAATGGGGTCTCTGCAAGGGGCAGACGAGGTGGGGGTGTCTTGTCGGACAGAAGGGGCTCTAATCCATCTGGTAGGCAAGCACTGGAAAGTAGTGATGACGAGGATGAAGATGAATCTGAAGATGTCTGGGAGAACGAAACTTCTTCGTCTCCTCGAAGATACCACAAAACAAATGATTTTGACAAAATAAATAGTAATAGAAGGCAGACCCAATCCAAACGTCCGGCTGAAGAGGAACTGGATATGGATAACTCGTCGTAACCGGGGTATAACTAAGTCTGGTCGGCCGAAGCGCGGCAGAAATAATGATAATGATGGATTATCAAGGACTTCTAGAGGTAATGGGGTCTCTGCAAGAGGCGGACGAGGTAGTGGGGTCTCTGCAAGAGGCTCTAAATCCTCTGGTAGGCAAGTACTGAAGATCAGTGATGACGAAGACGAAGACGAGTCTGAAGATGAAGATAAAGATGATGAATTTGATGCTGTTACGGATGTTACATCGAGTAGCGAAGATGATGATGAATTTGATTCAGCGAAGCCTGTGGGGCACAAGCAGCCAATGTTGAGTGCTGTTCAAAAAGCGGATCCAAAGACAAGCAGTGTGAAGGTTACTGGGGATGCTAATTATTTGAGCCAGTCAAGATTTGACCAGCACAATATCTCACCATTGACACTCAAGGCAACCAATGCAGTGGGGTATGAGCGCATGACCATTGTGCAAGAAGCAACTCTTCCATCTATTCTCAAAGGCAAGGATGTGTTGGCCAAGGCTAAAACTGGCACCGGCAAGACAGTAGCATTTCTGCTTCCTGCTATTGAGGTGATAGTGAAGTCACCACCGGTCCTCCGAGATGAGAAAAGTCCGCCTATTCTTGTTCTTGTCATTTGTCCCACTCGGGAACTTGCAAGTCAAGCTGCTGCCGAGGCCAATGTTTTGTTGAAATTCCATGCAAATATTGGTGTACAAGTAGTTACTGGGGGCACTAACTTACCAAAAGAACAGAAACGACTACAAACAAATCCTTGCCAGATATTAGTGGCCACTCCTGGAAGGCTTCGGGACCATATTGAGAATACCCCTGGTTTCTCAAGGCGTTTGTTGGGTGTGAAAGTGCTTGTACTTGATGAAGCTGACCATTTGCTAGATATGGGGTTCCGTAAGGATCTTGATAAGATAATTGCTGCTGTGCCTAAAGAGAGGCAGACACTCCTATTTTCAGCTACAGTTCCAGAAGAGGTTCACAAAGTATCCCAGATTGCTTTAAAACGGGATCATGAGTTTATTAATACAGTTGAAGAGGGCAGTGAGGAAACACACTCAAAGGTTTGTCAGTCATCAATTATTGCTTCACATGAGAGGCATTTCAACATTGTTTATGCTGTTCTGAAAGGGCACATCTCACAGGATCCTGACTACAAGGTCCTTATTTTTTGTACAACTGCAAGGGTTACTAGACTTGTGGCTGAACTTCTGGCGGAGTTGAAATTTAATGTCCGTGAGATTCACTCAAGAAAGTCTCAGGGTTATCGGACACGTATCTCTAATGAGTTTAGGATTTCAAAAGGTCTTATACTTGTTACTTCAGATGTCTCAGCTCGTGGAGTTGATTATCCTGATGTCACTCTTGTTGTTCAGATGGGAATACCTTCTGACCGACAACAGTACATGACACCGACTTGGCAGAACTGGACGCAAGGGTAAGGAGGGACAGGGTATTTTGTTACTTGCGCCATGGGAGGAGTACTTCTTAAATAGTGTGAAAGATTTACCCATCACAAAGGATCCAGTACCTCTTCTAGATTCAGAAACGAATTTGAAGGTGCAGCGAGCACTATCCCATATTGACTTGAACGATAAAGAATCTGCATATCAAGCATGGCTAGGATATTACAATTCTGTGAAACAGATTGGACGAGATAAGAGTAGGCTTGTTGAACTTGCAAATGAATTTAGTCGTTCTATGGGATTGGACAAACCACCAGCAATTGCAACATCAACCCTGTCCAAAATGGGCCTGAAGAATGTTCCAGGAATACGGACAAAATAGTGGGCAAGTTTGGATGGTGCATTATTCTGCTACAGTCATGATAATGGCCTTCTGAGAGCAGTCTGCTTCACACCGATCATTGGGCATGGAATCTAGCCATGGAATTTCCCTCACTCTACCAAGTCAGCTTGTCTCAGTGCTAAGCTATCACAAGTGTCCCTCGGATTTCATTTGCACTTATTATATGGGGCAAGATGCGGAAGTGTGTACATTCATAGAGGCTTTTGTAATGTGTCAATGTTCCCATAGAGAAGCGGGGATTGTTTGAATCAATCCTTACTATTTCAAGAATGTAATGTTTGGAGAAATACGATGCAGTCAAACCAGACCTTTGCAATTTTGGTGTTCCAAAACTATAATTTGAATTTTGAAGCTCCAATTCCAGTGCAGAACCTTTGGGCTGTTGCACATTAGTATTCTTTAATAGGACCTTGTTTAGTGGACTTGAACATTGTCAGTCAGGTAATCTATTTTGTTTTACTATCTGTAGTTATTATTATTGTCCAATTCAAATTGCTCTGTATTTCCTCGTCTTCAC

Ace file of the UniGene sp_v1.1_unigene2208

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	DEAD box RNA helicase (RH26)	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	33%
Blast2go	rna helicase	-	-	85%

Source	GOs	Term	Type	Identity
Blast2go	response to water deprivation	GO:0009414	Biological Process	85%
Blast2go	response to cold	GO:0009409	Biological Process	85%
Blast2go	response to salt stress	GO:0009651	Biological Process	85%
Blast2go	RNA binding	GO:0003723	Molecular Function	85%

Source	InterPros	Term	Type	Identity
Blast2go	Helicase, C-terminal	IPR001650	-	85%
Blast2go	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	85%
Blast2go	Helicase, superfamily 1/2, ATP-binding domain	IPR014001	-	85%
Blast2go	RNA helicase, DEAD-box type, Q motif	IPR014014	-	85%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKI3

Fln msg: query STOP codon too far from subject stop. Distance to subject end: 42 aas, putative chimeric sequence, your sequence is shorter than subject: 381 - 477

Fln protein: MLSAVQKADPKTSSVKVTGDANYLSQSRFDQHNISPLTLKATNAVGYERMTIVQEATLPSILKGKDVLAKAKTGTGKTVAFLLPAIEVIVKSPPVLRDEKSPPILVLVICPTRELASQAAAEANVLLKFHANIGVQVVTGGTNLPKEQKRLQTNPCQILVATPGRLRDHIENTPGFSRRLLGVKVLVLDEADHLLDMGFRKDLDKIIAAVPKERQTLLFSATVPEEVHKVSQIALKRDHEFINTVEEGSEETHSKVCQSSIIASHERHFNIVYAVLKGHISQDPDYKVLIFCTTARVTRLVAELLAELKFNVREIHSRKSQGYRTRISNEFRISKGLILVTSDVSARGVDYPDVTLVVQMGIPSDRQQYMTPTWQNWTQG*

Protein Length: 382

Fln nts: ATGGGGGGATTCCACGTTCCTCTACTTCCACATTCCCCAAAGGGTGGGTGTAATTGATACAAATATTTGCTTTGAGCACCATCCACTCATTTGCCCCCAACAACCTAATCTTCCCCTTTAAACCCTACCAAAACCCTACATATGCGCACGAAGGAAAATATCTTCGAACTCTTCCTCCCCCTCAACCAAAACCCTGCATATCCCGACGAAGGAAAATATCTTCGAACTCTTCCTCCCCCTCAAGTCTCCACGTTGTCCGCAGATCGACAGCTTGGGGAAGCCCTGCACTGTTGGGTTGGAGAGTGAGTTTGAAGTTAGCTACTAATCCACAATGGCGGGACGTCTTGGAGGAGCGCTTTTGCTGTACCCTACAGTTTCAACCATCAACAACATTTCGTGTAAAAATCTCAATCCTTGCTTCGCCTCTCAAGCATTCACGCTTTCTCCGAGTGCTTTTTCTTTAAATTTCCATATGTTTAGTATTGTAAGGAACGCCAAATTTACCCTGAGGCATTTCAATCCAAGTTCCAGAAAGAATAATTCAGTTGTTTTGGGTACCAGAAATCACTCAAGCCGCTCGTTTCGTTCTGAACGGGGTCGGGACGACGAAACTATCCCAGCTTGGCGAAGAACCCCTAAAACAACGACTTTTAACGCAAAAAGTACAAATAGACCAGAATCCAAAAGTCTGGTTGAAGAGGAACTGGATATGGATAACTTGTTTGGAAAACGGGGTGCATCTATGTCTGGTCGGTCGAATCCTGGTAGAAATGATTATAATGATGGATCGTCGAGGAATTATAGAGGCAATGATGTCTCTGCAAGGGGCAGACAAGGAGGGAAAGGCTCTTTTAATGGGGTCTCTCCAAGGGGCAGGCGAGGAGGGAATAGCTCGTTTAATGGGGTCTCTGCAAGGGGCAGACGAGGTGGGGGTGTCTTGTCGGACAGAAGGGGCTCTAATCCATCTGGTAGGCAAGCACTGGAAAGTAGTGATGACGAGGATGAAGATGAATCTGAAGATGTCTGGGAGAACGAAACTTCTTCGTCTCCTCGAAGATACCACAAAACAAATGATTTTGACAAAATAAATAGTAATAGAAGGCAGACCCAATCCAAACGTCCGGCTGAAGAGGAACTGGATATGGATAACTCGTCGTAACCGGGGTATAACTAAGTCTGGTCGGCCGAAGCGCGGCAGAAATAATGATAATGATGGATTATCAAGGACTTCTAGAGGTAATGGGGTCTCTGCAAGAGGCGGACGAGGTAGTGGGGTCTCTGCAAGAGGCTCTAAATCCTCTGGTAGGCAAGTACTGAAGATCAGTGATGACGAAGACGAAGACGAGTCTGAAGATGAAGATAAAGATGATGAATTTGATGCTGTTACGGATGTTACATCGAGTAGCGAAGATGATGATGAATTTGATTCAGCGAAGCCTGTGGGGCACAAGCAGCCA_-_ATGTTGAGTGCTGTTCAAAAAGCGGATCCAAAGACAAGCAGTGTGAAGGTTACTGGGGATGCTAATTATTTGAGCCAGTCAAGATTTGACCAGCACAATATCTCACCATTGACACTCAAGGCAACCAATGCAGTGGGGTATGAGCGCATGACCATTGTGCAAGAAGCAACTCTTCCATCTATTCTCAAAGGCAAGGATGTGTTGGCCAAGGCTAAAACTGGCACCGGCAAGACAGTAGCATTTCTGCTTCCTGCTATTGAGGTGATAGTGAAGTCACCACCGGTCCTCCGAGATGAGAAAAGTCCGCCTATTCTTGTTCTTGTCATTTGTCCCACTCGGGAACTTGCAAGTCAAGCTGCTGCCGAGGCCAATGTTTTGTTGAAATTCCATGCAAATATTGGTGTACAAGTAGTTACTGGGGGCACTAACTTACCAAAAGAACAGAAACGACTACAAACAAATCCTTGCCAGATATTAGTGGCCACTCCTGGAAGGCTTCGGGACCATATTGAGAATACCCCTGGTTTCTCAAGGCGTTTGTTGGGTGTGAAAGTGCTTGTACTTGATGAAGCTGACCATTTGCTAGATATGGGGTTCCGTAAGGATCTTGATAAGATAATTGCTGCTGTGCCTAAAGAGAGGCAGACACTCCTATTTTCAGCTACAGTTCCAGAAGAGGTTCACAAAGTATCCCAGATTGCTTTAAAACGGGATCATGAGTTTATTAATACAGTTGAAGAGGGCAGTGAGGAAACACACTCAAAGGTTTGTCAGTCATCAATTATTGCTTCACATGAGAGGCATTTCAACATTGTTTATGCTGTTCTGAAAGGGCACATCTCACAGGATCCTGACTACAAGGTCCTTATTTTTTGTACAACTGCAAGGGTTACTAGACTTGTGGCTGAACTTCTGGCGGAGTTGAAATTTAATGTCCGTGAGATTCACTCAAGAAAGTCTCAGGGTTATCGGACACGTATCTCTAATGAGTTTAGGATTTCAAAAGGTCTTATACTTGTTACTTCAGATGTCTCAGCTCGTGGAGTTGATTATCCTGATGTCACTCTTGTTGTTCAGATGGGAATACCTTCTGACCGACAACAGTACATGACACCGACTTGGCAGAACTGGACGCAAGGGTAA___GGAGGGACAGGGTATTTTGTTACTTGCGCCATGGGAGGAGTACTTCTTAAATAGTGTGAAAGATTTACCCATCACAAAGGATCCAGTACCTCTTCTAGATTCAGAAACGAATTTGAAGGTGCAGCGAGCACTATCCCATATTGACTTGAACGATAAAGAATCTGCATATCAAGCATGGCTAGGATATTACAATTCTGTGAAACAGATTGGACGAGATAAGAGTAGGCTTGTTGAACTTGCAAATGAATTTAGTCGTTCTATGGGATTGGACAAACCACCAGCAATTGCAACATCAACCCTGTCCAAAATGGGCCTGAAGAATGTTCCAGGAATACGGACAAAATAGTGGGCAAGTTTGGATGGTGCATTATTCTGCTACAGTCATGATAATGGCCTTCTGAGAGCAGTCTGCTTCACACCGATCATTGGGCATGGAATCTAGCCATGGAATTTCCCTCACTCTACCAAGTCAGCTTGTCTCAGTGCTAAGCTATCACAAGTGTCCCTCGGATTTCATTTGCACTTATTATATGGGGCAAGATGCGGAAGTGTGTACATTCATAGAGGCTTTTGTAATGTGTCAATGTTCCCATAGAGAAGCGGGGATTGTTTGAATCAATCCTTACTATTTCAAGAATGTAATGTTTGGAGAAATACGATGCAGTCAAACCAGACCTTTGCAATTTTGGTGTTCCAAAACTATAATTTGAATTTTGAAGCTCCAATTCCAGTGCAGAACCTTTGGGCTGTTGCACATTAGTATTCTTTAATAGGACCTTGTTTAGTGGACTTGAACATTGTCAGTCAGGTAATCTATTTTGTTTTACTATCTGTAGTTATTATTATTGTCCAATTCAAATTGCTCTGTATTTCCTCGTCTTCAC

Fln Alignment:

sp_v1.1_unigene2208___KADPKTSSVKVTGDANYLSQSRFDQHNISPLTLKATNAVGYERMTIVQEATLPSILKGKDVLAKAKTGTGKTVAFLLPAIEVIVKSPPVLRDEKSPPILVLVICPTRELASQAAAEANVLLKFHANIGVQVVTGGTNLPKEQKRLQTNPCQILVATPGRLRDHIENTPGFSRRLLGVKVLVLDEADHLLDMGFRKDLDKIIAAVPKERQTLLFSATVPEEVHKVSQIALKRDHEFINTVEEGSEETHSKVCQSSIIASHERHFNIVYAVL-KGHISQDPDYKVLIFCTTARVTRLVAELLAELKFNVREIHSRKSQGYRTRISNEFRISKGLILVTSDVSARGVDYPDVTLVVQMGIPSDRQQYM
B8LKI3______________KIPPPDSRYK-TEDVTATKGNEFEDYFLKRELLMGIYEKGFERPSPIQEESIPIALTGSDILARAKNGTGKTAAFCIPALEKI--------DPKKNSIQVLLLVPTRELALQTSQVCKELAK-HLKIQIMVTTGGTSLKDDIMRLY-QPVHILVATPGRV---LDLTKKGVCNLKDCAMLVMDEADKLLSPEFQPLVEQLIGFLPENRQILLYSATFPVTVKSFKDKYLRKPY-VINLMD---ELTLKGITQFYAFVEERQKVHCLNTLFSKLQINQS-----IIFCNSVNRVELLAKKITELGYSCFYIHAKMLQSHRNRVFHDFRNGACRNLVCSDLFTRGIDIQAVNVVINFDFPKNSETYL

SNPs (tot: 43)

Position	A %	C %	G %	T %	Probability
364	33		66		1.0
376		12		87	0.992554
379	85			14	0.993533
399	12		87		0.992554
403	87			12	0.992554
404	87	12			0.992554
412			12	87	0.992554
421		42		57	1.0
615	66	33			0.997452
697		20	80		0.995493
703	80	20			0.995493
704		20	80		0.995493
705	80			20	0.995493
706			80	20	0.995493
708	80		20		0.995493
713			80	20	0.995493
718	80	20			0.995493
719	20			80	0.995493
764		80		20	0.995493
770	20	80			0.995493
825		85	14		0.993533
826	14			85	0.993533
829		14	85		0.993533
830	16	83			0.994512
852	75			25	0.996473
1062	66		33		0.997452
1603	16		83		0.994512
1607			33	66	1.0
1773		83		16	0.994512
2322	71		28		1.0
2643	33		66		1.0
3007		12		87	0.992554
3048	83	16			0.999991
3049	83		16		0.999991
3050	75			25	1.0
3053	83		16		0.999991
3054	16		83		0.999991
3057	76		7	15	0.999983
3061	78	7			0.999974
3062	71	21	7		1.0
3064		7	14	78	0.999974
3067	14	78		7	0.999974
3460	40		60		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain