SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene2014

Length: 2048 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene2014
CCTACGTTCCATGGGGATTGGGAAGAACGAACCTGCGGTAATTCCTAAACTTCTTGGGCGTTTCCGAGCCATAATATTTCTTGAATACCTAAACTTCTCGGGCGTCTCCCCGGCTCGACGCTATTTTCAGGCGGTGGTGATTCTCATACATCGGAGGATTCCATACCGAGATATTCTGAAGTTTCGTTTCTCATAGCACTTCGGTACCTTACAGAAAAGCAGTTTGGAATCAGTAGAGGAAGTTGAAGGTTGACAAAATGGTTGGCACCACAGAAACGAGGATGGAGCAGCAATTTCCTCCTCATGCTCCAGTTAACAATATTATTTGTCTGATGACGGACCCAGACGGTAACGCCCTAGGAGGTCCGGTTGATCTTCCCCAGGACGCAGGGCCGCTTCAGCTGCAGTCTCTCGTCAATGCTCTGCTAAATAACGAAGAGAAGCTTCCTTATGCCTTTTATATTGCTGATCAGGAACTTGTTGGTCAACTTGGTGTATATTTGTTGAAGAATAAAGTATCTGTGGAACAAGTATTACGGATAGTGTATCAGCCTCAAGCCATTTTTCGAATTCGCCCAGTTAGTCGCTGCTCATCTACAATTGCTGGGCATACAGAGGCTGTTCTTTCAGTAGCATTCAGCCCAGATGGACGACATCTGGCGAGTGGTTCTGGTGATACTACTGTTCGATTCTGGGACCTTAATACTCAATCTCCATTATACACATGCACAGGGCACAAAAACTGGGTTCTTTGCATTTCATGGTCACCGGATGGGAAACAACTTGTCAGTGGAAGCAAAGATGGAGACCTACGAATTTGGGACCCTGAGACTGGGAGGGCATTAGGCAATCCACTTAATGGCCACAAGAAGTGGATTACTGGGCTTGCTTGGGAACCTGTACATCTGCAAGCCCCTTGTCGCCGCTTTGCCAGTTCAAGTAAAGATGGAGATGTTCGTGTTTGGGATACTGTCCTAAGGAAGTGTGTGATGTCCCTAACTGGCCATACACTTGCAGTGACTTGTGTGAAATGGGGAGGTGATGGGATGATCTATTCCAGTTCGCAGGACTGCACAATCAAAGTTTGGGAAACTTCTCAGGGGAAGCTTATTCGTGAATTAAGGGGGCATGGACATTGGGTTAATACTCTTGCTTTGAGCACAGAGTATGCACTTCGAACAGGGCCCTATGATCATAAAGGGAAGCAGTATTCTTCAGCTGAAGAAATGAAAAAGGCTGCATTGGAAAAATACAATGCTGCCAAAAGGGATGCTCCAGAGAGACTGGTTTCTGGGTCTGATGATTTTACAATGTTTCTTTGGGAGCCTGCATCTAGTAAACAACCAAAGGCACGAATGACAGGGCATCAGCAGCTTGTCAACCATGTTTACTTCTCACCTGATGGGCAATGGGTGGCAAGTGCCTCATTTGATAAATCTGTAAAGTTATGGAATGGCATTACAGGGAAGTTTGTTACAACATTCCGTGGGCATGTCGGCCCAGTGTATCAAATCAGTTGGTCTGCGGATAGTAGACTGTTGCTAAGTGGAAGCAAGGATTCAACACTTAAGCTTTGGGACATGCGCACTCTGAAGTTGAGAGAAGATCTTCCGGGTCATGCAGACGAGGTGTTTTCCGTGGACTGGAGTCCTGATGGTGAGAAGGTAGCATCTGGTGGGAAAGACCGTTTGCTGAAACTATGGATGAGTTGATAACGTGGAATGGGGGAGTTACATATTGGATTCTGGAACTCTGATCAGCCTATGGCATAGAAGGTTACATTTACAATTGCGGTTCTTTGTTCAAACAGTGGGCTAGGTCTGTGCTGGGATTATATTCATTTTGTCTGTTCATTGATTGAGAAATTCTTTAACATGACTATTGAAATATAAGCAGGGCATTTATTTTTAGGGCAGCGTGCTTCAGTTTATATTACAATGGAAAGGCATGTAGAAATATCATTGACAAGCAATTCTCAATGTTCATGAAATTCACAATGTGCATCTTTTTGTAAAAGGATTTATTTATTGAAGTAAATTACAACCTAG

Ace file of the UniGene sp_v1.1_unigene2014 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	NLE multi-domain protein	-	-	6.0e-13	32%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	27%
Blast2go	protein	-	-	0.0	86%

Source	GOs	Term	Type	e value	Identity
Blast2go	transferase activity	GO:0016740	Molecular Function	0.0	86%

Source	InterPros	Term	Type	Identity
Blast2go	G-protein, beta subunit	IPR001632	-	86%
Blast2go	WD40 repeat	IPR001680	-	86%
Blast2go	WD40 repeat-like-containing domain	IPR011046	-	86%
Blast2go	NLE	IPR012972	-	86%
Blast2go	WD40/YVTN repeat-like-containing domain	IPR015943	-	86%
Blast2go	WD40-repeat-containing domain	IPR017986	-	86%
Blast2go	WD40 repeat, conserved site	IPR019775	-	86%
Blast2go	WD40 repeat, subgroup	IPR019781	-	86%
Blast2go	WD40 repeat 2	IPR019782	-	86%
Blast2go	G-protein beta WD-40 repeat	IPR020472	-	86%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: A9NPC1

Fln msg: your sequence is longer than subject: 485 - 314

Fln protein: MVGTTETRMEQQFPPHAPVNNIICLMTDPDGNALGGPVDLPQDAGPLQLQSLVNALLNNEEKLPYAFYIADQELVGQLGVYLLKNKVSVEQVLRIVYQPQAIFRIRPVSRCSSTIAGHTEAVLSVAFSPDGRHLASGSGDTTVRFWDLNTQSPLYTCTGHKNWVLCISWSPDGKQLVSGSKDGDLRIWDPETGRALGNPLNGHKKWITGLAWEPVHLQAPCRRFASSSKDGDVRVWDTVLRKCVMSLTGHTLAVTCVKWGGDGMIYSSSQDCTIKVWETSQGKLIRELRGHGHWVNTLALSTEYALRTGPYDHKGKQYSSAEEMKKAALEKYNAAKRDAPERLVSGSDDFTMFLWEPASSKQPKARMTGHQQLVNHVYFSPDGQWVASASFDKSVKLWNGITGKFVTTFRGHVGPVYQISWSADSRLLLSGSKDSTLKLWDMRTLKLREDLPGHADEVFSVDWSPDGEKVASGGKDRLLKLWMS*

Protein Length: 486

Fln nts: CCTACGTTCCATGGGGATTGGGAAGAACGAACCTGCGGTAATTCCTAAACTTCTTGGGCGTTTCCGAGCCATAATATTTCTTGAATACCTAAACTTCTCGGGCGTCTCCCCGGCTCGACGCTATTTTCAGGCGGTGGTGATTCTCATACATCGGAGGATTCCATACCGAGATATTCTGAAGTTTCGTTTCTCATAGCACTTCGGTACCTTACAGAAAAGCAGTTTGGAATCAGTAGAGGAAGTTGAAGGTTGACAAA_-_ATGGTTGGCACCACAGAAACGAGGATGGAGCAGCAATTTCCTCCTCATGCTCCAGTTAACAATATTATTTGTCTGATGACGGACCCAGACGGTAACGCCCTAGGAGGTCCGGTTGATCTTCCCCAGGACGCAGGGCCGCTTCAGCTGCAGTCTCTCGTCAATGCTCTGCTAAATAACGAAGAGAAGCTTCCTTATGCCTTTTATATTGCTGATCAGGAACTTGTTGGTCAACTTGGTGTATATTTGTTGAAGAATAAAGTATCTGTGGAACAAGTATTACGGATAGTGTATCAGCCTCAAGCCATTTTTCGAATTCGCCCAGTTAGTCGCTGCTCATCTACAATTGCTGGGCATACAGAGGCTGTTCTTTCAGTAGCATTCAGCCCAGATGGACGACATCTGGCGAGTGGTTCTGGTGATACTACTGTTCGATTCTGGGACCTTAATACTCAATCTCCATTATACACATGCACAGGGCACAAAAACTGGGTTCTTTGCATTTCATGGTCACCGGATGGGAAACAACTTGTCAGTGGAAGCAAAGATGGAGACCTACGAATTTGGGACCCTGAGACTGGGAGGGCATTAGGCAATCCACTTAATGGCCACAAGAAGTGGATTACTGGGCTTGCTTGGGAACCTGTACATCTGCAAGCCCCTTGTCGCCGCTTTGCCAGTTCAAGTAAAGATGGAGATGTTCGTGTTTGGGATACTGTCCTAAGGAAGTGTGTGATGTCCCTAACTGGCCATACACTTGCAGTGACTTGTGTGAAATGGGGAGGTGATGGGATGATCTATTCCAGTTCGCAGGACTGCACAATCAAAGTTTGGGAAACTTCTCAGGGGAAGCTTATTCGTGAATTAAGGGGGCATGGACATTGGGTTAATACTCTTGCTTTGAGCACAGAGTATGCACTTCGAACAGGGCCCTATGATCATAAAGGGAAGCAGTATTCTTCAGCTGAAGAAATGAAAAAGGCTGCATTGGAAAAATACAATGCTGCCAAAAGGGATGCTCCAGAGAGACTGGTTTCTGGGTCTGATGATTTTACAATGTTTCTTTGGGAGCCTGCATCTAGTAAACAACCAAAGGCACGAATGACAGGGCATCAGCAGCTTGTCAACCATGTTTACTTCTCACCTGATGGGCAATGGGTGGCAAGTGCCTCATTTGATAAATCTGTAAAGTTATGGAATGGCATTACAGGGAAGTTTGTTACAACATTCCGTGGGCATGTCGGCCCAGTGTATCAAATCAGTTGGTCTGCGGATAGTAGACTGTTGCTAAGTGGAAGCAAGGATTCAACACTTAAGCTTTGGGACATGCGCACTCTGAAGTTGAGAGAAGATCTTCCGGGTCATGCAGACGAGGTGTTTTCCGTGGACTGGAGTCCTGATGGTGAGAAGGTAGCATCTGGTGGGAAAGACCGTTTGCTGAAACTATGGATGAGTTGA___TAACGTGGAATGGGGGAGTTACATATTGGATTCTGGAACTCTGATCAGCCTATGGCATAGAAGGTTACATTTACAATTGCGGTTCTTTGTTCAAACAGTGGGCTAGGTCTGTGCTGGGATTATATTCATTTTGTCTGTTCATTGATTGAGAAATTCTTTAACATGACTATTGAAATATAAGCAGGGCATTTATTTTTAGGGCAGCGTGCTTCAGTTTATATTACAATGGAAAGGCATGTAGAAATATCATTGACAAGCAATTCTCAATGTTCATGAAATTCACAATGTGCATCTTTTTGTAAAAGGATTTATTTATTGAAGTAAATTACAACCTAG

Fln Alignment:

sp_v1.1_unigene2014___CSSTIAGHTEAVLSVAFSPDGRHLASGSGDTTVRFWDLNTQSPLYTCTGHKNWVLCISWSPDGKQLVSGSKDGDLRIWDPETGRALGNPLNGHKKWITGLAWEPVHLQAPCRRFASSSKDGDVRVWDTVLRKCVMSLTGHTLAVTCV----KWGGDGMIYSSSQDCTIKVWETSQGKLIRELRGHGHWVNTLAL-STEYALRTGPYDH
A9NPC1______________CIKTLKGHTNYVFCVNFNPQSNLIVSGSFDETVRIWDVRTGKCLKVLPAHSDPVTAVHFNRDGSLIVSSSYDGLCRIWDSATGHCLKTLIDDENPPVSFVNFSP-----NGKFILAGTLDNTLRLWNFATGKFLKTYTGHVNSKYCISSTFSVTNGKYIVSGSEDNCVYLWDLQARNIVQKLEGHTDTVISVSCHPTENKIASGALEN

SNPs (tot: 19)

Position	A %	C %	G %	T %	Probability
1649	37		62		1.0
1650	12	81	6		0.999943
1651	18			81	1.0
1652	18		81		1.0
1714	18		81		0.999994
1930	90			10	0.90298
1932	10	90			0.90298
1935		90	10		0.90298
1936	10		90		0.90298
1941	10		90		0.90298
1942	10		90		0.90298
1945	90			10	0.90298
1946		11	88		0.923564
1950	11	88			0.923564
1952		88	11		0.923564
2014		66		33	1.0
2023		83		16	0.994512
2031		40	60		1.0
2033	60	40			1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain