SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1972

Length: 1598 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene1972 (antisense)

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[R] COG5038 Ca2+-dependent lipid-binding protein, contains C2 domain	-	-	3.0e-07	27%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	27%
Blast2go	plant synaptotagmin	-	-	0.0	71%

Source	InterPros	Term	Type	Identity
Blast2go	C2 calcium-dependent membrane targeting	IPR000008	-	71%
Blast2go	C2 calcium/lipid-binding domain, CaLB	IPR008973	-	71%
Blast2go	C2 membrane targeting protein	IPR018029	-	71%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: B8LRA0

Fln msg: Unexpected stop codon in the beginning of your sequence, your sequence is shorter than subject: 450 - 536

Fln protein: WAQAP*N*IFMNKVQAMSNMIIWPAVKSR*IMLMMTPLYFGRLEGIQVCWLHVLNIVERKC*WLSPLRAQIQQRLKLS*YLENGSLLMVPEAFTVITHQVLTNLYLDPQIFIHQLAESLKITVVEGSNLAGKDKSRKNDPYIKLQYGKKIRKTRTISRAISPIWDEEFEFNEIEGDQCLKIKCYNGEFLGDENMGSARVNLEGLEEGETKDVWIPLEKVTTGEVRLKIEKQKQEQDTENSLAESGNGWIELVLVEARDLIAADWRGTSDPYVRVQYGNLKKRTKVVYKTLNPKWNQTLEFPDTGSRLVLHVKDHNAVLPTYSIGDCFVEYERLPPNEMVDKWIPLEGVTKGEIHVQVTRRVPEMSKKSSLSHQRSSMSKVHGLSEKVREIIEKVKSLAEDGDTENMSPYLDELESVEEEQEMCMFQLHLDKTTLLEKIHELDQVMKGVK*

Protein Length: 451

Fln nts: ATGGGCACAGGCACCCTAAAACTAAATCTTTATGAACAAGGTTCAAGCAATGTCAAATATGATTATTTGGCCAGCTGTGAAATCAAGATGAATTATGTTGATGATGACTCCACTATATTTTGGGCGATTGGAAGGGATTCAAGTATGTTGGCTGCACGTGCTGAATATTGTGGAAAGGAAGTGCTGATGGTTGTCCCCTTTGAGGGCACAGATTCAGCAGAGATTAAAGTTAAGCTAGTACTTAGAGAATGGCAGTTTGCTGATGGTTCCAGAAGCCTTCACAGTCATAACACATCAAGTTCTGACCAACCTTTACTTGGATCCTCAAATTTTCATTCACCAACTGGCAGAAAGCCTTAAAATAACAGTAGTGGAAGGCAGCAACCTTGCTGGAAAGGACAAATCTCGAAAGAATGATCCTTACATCAAGCTTCAGTATGGGAAGAAAATTCGCAAAACAAGAACGATTTCACGAGCGATCAGCCCCATATGGGATGAGGAATTTGAGTTTAACGAGATTGAAGGTGATCAGTGTTTGAAAATAAAATGTTACAATGGAGAATTTCTTGGGGATGAGAACATGGGAAGTGCCCGTGTGAATTTGGAAGGACTGGAAGAAGGAGAAACTAAGGATGTTTGGATCCCTCTTGAAAAAGTAACGACAGGTGAAGTAAGACTCAAAATTGAGAAGCAAAAGCAAGAACAAGACACTGAGAACTCCCTTGCAGAATCAGGGAATGGATGGATTGAGCTTGTTCTTGTAGAAGCAAGGGATCTGATTGCAGCAGACTGGCGTGGAACCAGTGACCCATATGTTAGGGTACAGTATGGCAATTTGAAAAAAAGAACAAAGGTTGTCTATAAGACACTCAATCCTAAATGGAATCAAACCTTGGAGTTCCCTGACACTGGTAGCCGCTTGGTGCTTCATGTAAAGGATCATAATGCTGTATTACCAACATACAGCATCGGTGATTGTTTTGTAGAGTATGAAAGACTACCTCCAAATGAAATGGTTGACAAATGGATTCCTTTAGAAGGAGTTACGAAAGGAGAAATACATGTTCAAGTGACAAGAAGAGTGCCAGAAATGTCGAAAAAATCAAGTTTGTCACACCAAAGATCCAGCATGTCAAAAGTGCACGGGTTATCTGAAAAGGTCCGCGAAATTATAGAAAAAGTTAAATCCTTGGCAGAAGATGGGGATACTGAAAATATGTCACCATATTTGGATGAATTAGAAAGTGTTGAAGAAGAACAAGAAATGTGCATGTTCCAACTCCACTTAGACAAGACTACATTACTGGAAAAAATTCATGAACTGGACCAAGTGATGAAGGGAGTTAAATAG___GGCTTATGTACTGCAGTAAAATTAACACAACATTGATCTTACGTGTTACTTAGAAACATGGATACAGAGAATTTACGTGATATACATTCATTTTATGTACTTTAGCACAGGTTCCAAAGGATACCAGGCTGGTCTCTTCTTTGCTGTCTGTGTTTCAGAGTACTATTAAGAAATGTATAATATTCATAGTTAAGGAACAATGTAATCGTTTCTTCTCTTTTGTAGTGTAGAATGTAAATCAAGGATT

Fln Alignment:

sp_v1.1_unigene1972___LENGSLLMVPEAFTVITHQV---LTNLYLDPQIF---------IHQLAESLKITVVEGSNLAGKDKSRKNDPYIKLQY-GKKI--RKTRTISRAISPIWDEEFEFNEIEGD-QCLKIKCYNGEFLGD-ENMGSARVNLEGLEEGETKDVWIPLEKV----------TTGEVRLKI------EKQKQEQDTENSLAE-------SGNGWIELVLVEARDLIAADWRGTSDPYVRVQYGNLKKRTKVVYKTLNPKWNQTLEF----PDTGSRLVLHVKDHNAVLPTYS
B8LRA0______________LLGGDLMAIPGLYQFAQEMIKEQVANLYLWPKTLEVPILDQRATHKPVGMLHVKVVRAINLKKKDMLGKSDPYVKLKMTGEKLPSKKTTVKSSNLNPEWNEEFKFVVKDPESQALELSVYDWEKVGSHEKMGIQTYDLKELTPSETKSVTLNLLKSLDPNDPQNAKARGQITIEMTYNPFKEDENSPADDEDSVVEKAPEGTPAGGGLLVVRVLEAEDV---EGKHHTNPYVRLLFKGEEKKTKPVKKNRDPRWDQEFEFMLEDPPVNDKIHVEVMSKGSSLALHS

SNPs (tot: 3)

Position	A %	C %	T %	Probability
1236	80	20		0.995493
1238		20	80	0.995493
1381		25	75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain