SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1941

Length: 1725 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene1941

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	ATP binding / ATP-dependent DNA helicase/ ATP-dependent helicase/ helicase/ nucleic acid binding n=1 Tax=Arabidopsis thaliana RepID=UPI00017390E4	-	-	53%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	82%
Blast2go	atp-dependent dna helicase	-	-	72%

Source	InterPros	Term	Type	e value	Identity
Blast2go	DNA/RNA helicase, DEAD/DEAH box type, N-terminal	IPR011545	-	0.0	72%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AC88

Fln msg: Overlapping hits, possible frame ERROR between 284 and 277, your sequence is longer than subject: 434 - 394

Fln protein: MSRLLAGDVGCGKTIVALLAALEVIDSGYQVAFMVPTELLAMQHHGNIISILERINKDDRPSVALLTGSTPLKEARLIQKGLEMGDxxxxVVGTHKLFSETVNFASLCFSVIDEQHRFGVEQKGRFSSKACTVMNKSVANGNKLEDGQLVANKTFFAPHVLVMSATPIPRTMALALHGNMSLSKINELPPGRIPVKTLALHGNKVGLTKTYQMVQKDLDKGGRVFLVYSAIDESEQLPELHAAAAEINKISNVFKGYKCGLVHGRMNHTEKEAAIQQFKEGKTQVLLSTQVVEVGIDIPEATMMVIMNAERFGIAQLHQLRGRVGRGTGKSTCILVSSSAIGLPRLKILQQSMDGFELAEADLRLRGPGSLLGTRQSGYLPEFSIARLQTDGDVLEQAHFAAVDILQQHTRLDSLPMLKAEISMRYPLNSLGA*

Protein Length: 435

Fln nts: TCAGAAGGCCAGTACCA_-_ATGAGTCGTCTTCTAGCGGGTGATGTTGGTTGTGGAAAAACTATTGTAGCACTTTTGGCTGCTCTAGAGGTTATCGATTCAGGGTATCAGGTAGCATTTATGGTGCCAACAGAATTATTAGCAATGCAGCATCATGGGAACATTATTTCTATTCTAGAGAGGATAAACAAAGATGACAGGCCTTCAGTAGCTCTTTTAACTGGTTCCACTCCATTAAAGGAAGCTCGTTTAATACAGAAGGGATTGGAGATGGGAGATxxxxxxxxxxxxGTAGTAGGCACACACAAATTGTTTTCAGAAACTGTGAATTTTGCCTCTCTTTGCTTTTCAGTAATAGATGAACAACATCGCTTTGGAGTGGAACAAAAGGGAAGGTTTAGTAGCAAGGCTTGCACAGTTATGAACAAGTCAGTTGCAAATGGCAATAAGTTAGAAGATGGGCAGTTGGTTGCAAATAAGACATTCTTTGCTCCACATGTTCTTGTCATGTCTGCCACTCCAATTCCTAGGACCATGGCTTTAGCTCTTCATGGAAATATGTCACTCAGTAAGATAAATGAACTTCCACCAGGTCGAATTCCTGTGAAAACACTTGCACTCCATGGAAACAAAGTGGGGCTCACTAAAACATACCAGATGGTCCAGAAAGATCTTGACAAAGGAGGAAGAGTTTTTCTTGTTTACTCAGCAATAGATGAAAGTGAACAGCTTCCAGAACTTCATGCAGCTGCTGCAGAAATCAATAAAATATCTAATGTCTTTAAGGGTTACAAATGTGGTCTCGTTCATGGTCGTATGAATCATACTGAAAAAGAAGCAGCAATTCAACAGTTCAAAGAAGGAAAGACACAAGTTTTGCTCTCAACCCAGGTGGTTGAAGTTGGAATTGACATTCCTGAGGCAACAATGATGGTAATTATGAATGCTGAGAGGTTTGGAATAGCACAGTTGCATCAGCTCAGAGGTCGTGTTGGAAGGGGCACTGGAAAATCTACTTGTATACTTGTCTCATCCTCTGCAATTGGATTACCACGGCTGAAAATATTGCAGCAGTCAATGGATGGATTTGAATTGGCAGAAGCAGATCTCCGTCTTCGCGGCCCTGGAAGCTTGCTTGGCACGCGACAGTCAGGATACCTTCCAGAATTTTCTATTGCTCGGCTTCAGACTGACGGTGATGTTCTTGAGCAAGCTCATTTTGCAGCAGTGGACATTCTTCAACAGCACACTCGCTTGGACAGCCTGCCGATGCTCAAAGCCGAAATCAGTATGCGGTATCCTCTCAATTCACTTGGTGCATAG___GCTTCCTAGGAGCGAGTTTGTAAATCTGTAGTTGCTTACAAAGTGAGATGACAACTATGAAGTAGTTTCTCTACATCATATTCCAATTGTAATGGTTTTTGGGAAAATCGTTTTGTAGCCCATGTACAGCTTTATACCGTGTTTAGAGCCATATTTTAAAGAAAATTGTTTATTGTTCATTATTCTTCAAGTAATGGAAAATGTCGTTTCAATATTCAAAGGGAAATGAATGCAGGTCTTCTCCATATTCCTTGTTTAGGTTCTTTAAATTTGATATTCTAAGCTTAAACTTTTGGACCATGTATTCATGCTTTATGGAAAGTATCAGACATGCAGCAGATAAAGATTGCCGTCTATTTTAATCTGATTTTGACCAACTTTTCATGCTTCCTAGAACTCAGAGGTAGA

Fln Alignment:

sp_v1.1_unigene1941___MVPTELLAMQHHGNIISILERINKDDRPSVALLTGSTPLKEARLIQKGLEMGDxxxVVGTHKLFSETVNFASLCFSVIDEQHRFGVEQKGRFSSKACTVMNKSVANGNKLEDGQLVANKTFFAPHVLVMSATPIPRTMALALHGNMSLSKINELPPGRIPVKTLALHGNKVGLTKTYQMVQKDLDKGGRVFLVYSAIDESEQLPELHAAAAEINKISNVFKGYKCGLVHGRMNHTEKEAAIQQFKEGKTQVLLSTQVVEVGIDIPEATMMVIMNAERFGIAQLHQLRGRVGRGTGKSTCILVSSSAIGLPRLKILQQSMDGFELAEADLRLRGPGSLLGTRQSGYLPEFSIARLQTDGDVLEQAHFAAVDILQQHTRLDSLPMLKAEISMRYPLNSLGA
D5AC88______________MVPTELLATQHYENIISMLERINKDDRPSVAFLTGSTPLKETRLIRKGLEMGDxxxVVGTHKLFSESVNFASLCFAVIDEQHRFGVEQKGRFSSKACTVMDKSGANGIKLEDGQLVANKTFFAPHVLVMSATPIPRTLALTLHGNMSLSKINELPPGRIPVKTLALHGNTKGLTKTYQMVQKELDSGGRVFLVYPAIDESEQLPELHAAAAEIKKISKVFKGYKCGLLHGRMKTTEKEKAIQQFKEGKTQILLSTQVVEVGIDIPEATMMVIMNAERFGISQLHQFRGRVGRGTGKSTCILVSSSAVGLPRLKILQQSMDGFELAEADLHLRGPGSLLGTRQSGHLPEFSIARLQTDGDVLEKAHFAAL-----HPCLDSLPMLKAEISMRHPLNSLGA

SNPs (tot: 21)

Position	A %	C %	G %	T %	Probability
380	80		20		0.995493
456	16		83		0.994512
822	60			40	1.0
990	20		80		0.995493
993			20	80	0.995493
1120		75		25	0.996473
1336	16		83		0.994512
1388	66	33			1.0
1399	85		14		0.993533
1402	83		16		0.994512
1404	83		16		0.994512
1405	20			80	0.995493
1406		20		80	0.995493
1408		80		20	0.995493
1409	80			20	0.995493
1410	80		20		0.995493
1411			20	80	0.995493
1416	80	20			0.995493
1420		33	66		0.997452
1421	33		66		0.997452
1422			33	66	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain