SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1920

Length: 1843 nt

Origin of reads: Biogeco_1 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene1920

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	tRNA delta(2)-isopentenylpyrophosphate transferase, putative (EC:2.5.1.8); K00791 tRNA dimethylallyltransferase [EC:2.5.1.75]	-	-	44%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	66%
Blast2go	trna isopentenyltransferase	-	-	59%

Source	ECs	Term	Type	e value	Identity
Blast2go	Transferred entry: 2.5.1.75.	EC:2.5.1.8	-	0.0	59%
Blast2go	Adenylate dimethylallyltransferase.	EC:2.5.1.27	-	0.0	59%

Source	KEGGs	Term	Type	e value	Identity
Blast2go	Zeatin biosynthesis	00908		0.0	59%
Blast2go	Biosynthesis of plant hormones	01070		0.0	59%

Source	ECs	Term	Type	e value	Identity
AutoFact	tRNA dimethylallyltransferase.	EC:2.5.1.75	-	0.0	44%

Source	KEGGs	Term	Identity
AutoFact	Zeatin biosynthesis	00908	44%
AutoFact	Biosynthesis of plant hormones	01070	44%
AutoFact	Metabolic pathways	01100	44%
AutoFact	Biosynthesis of secondary metabolites	01110	44%

Source	GOs	Term	Type	Identity
Blast2go	cytokinin biosynthetic process	GO:0009691	Biological Process	59%
Blast2go		GO:0004811	Molecular Function	59%
Blast2go	AMP dimethylallyltransferase activity	GO:0009824	Molecular Function	59%
Blast2go	cytosol	GO:0005829	Cellular Component	59%

Source	InterPros	Term	Type	e value	Identity
Blast2go	tRNA isopentenyltransferase	IPR002627	-	0.0	59%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LNK3

Fln msg: Warning!, your query overlaps and the subject is separated,

Fln protein: MEERLSKSPNANHKHKHKPKVVVIMGATGSGKSRLAIDLAQHFPAEILNADCMQVYEGLDIVTNKVPVEERKGVIHHLLGTVSSKVNFTSSDFRDTAIPIIDSIISRKHLPIIVGGSNSYIQALVSTVDDVMKNGDDDLKTSERPFGICSNAGSVNDLHKRCDNDVQNTSNQFDQVEDIDLITTNGLHSIDFSQGSADDLHKRCNDDVQNTSNKFDLIATNGVHPTDFSKGQKGDTADSFRYDCWFLRLDASLPVLDKFVEDRVDFMIEAGLLEEVSAFYDPRANYKHGLWQAxxxxxxxxxxxxxxxxxKAKGQEIEKLDKGSHKILLDAAIERMKINTCQLVRKQKQRISRLKTFSGWNLHQLDSTKALETSGSESLEHWEMMVVNISMDVVKRFLSERTCETSLKENVESRGDSMHPEQAQDLWIHSxxxLAEIKCFREHMNGRNINLVVHIEGKFCDLK*

Protein Length: 465

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATTCTTTGGAGAATTGAATTAGAAATATTTTGCGCAGAAGAAGATATGTGCAAAAAAAAAGTATTTAAAAAGGGGATGTGAATTGGGCTTTTCCGTAAGCATTGCAGTTACCACAGCAATTATCAATAACA_-_ATGGAAGAACGTTTATCTAAATCCCCCAACGCCAACCACAAGCACAAGCACAAGCCCAAGGTGGTGGTCATAATGGGAGCCACGGGGTCCGGAAAATCGCGTCTTGCGATTGATTTGGCTCAGCACTTCCCTGCCGAGATCCTAAATGCCGACTGCATGCAGGTCTACGAAGGTTTGGACATCGTAACCAACAAAGTGCCAGTCGAGGAAAGAAAGGGAGTAATTCATCATCTTCTTGGTACAGTCAGTTCCAAAGTCAACTTCACATCATCGGATTTCCGTGACACAGCTATTCCTATTATTGATTCAATCATCTCTCGGAAACACTTGCCTATCATTGTTGGTGGCTCAAACTCCTACATACAGGCTCTTGTAAGCACGGTAGATGATGTGATGAAGAATGGAGATGATGACCTCAAAACGAGCGAAAGACCTTTTGGCATTTGTAGTAATGCAGGAAGTGTCAATGACCTACATAAAAGATGTGACAATGATGTTCAAAATACAAGTAATCAATTTGATCAAGTTGAAGATATTGATTTAATTACAACCAATGGACTTCATTCTATTGATTTCAGTCAGGGAAGTGCCGATGATTTACATAAAAGATGCAATGATGATGTTCAAAATACAAGTAATAAATTTGATCTGATTGCAACTAATGGAGTTCATCCTACTGATTTTAGTAAGGGGCAGAAAGGGGATACAGCAGATTCTTTCAGATATGATTGCTGGTTTTTGCGGTTAGATGCTTCATTGCCTGTACTTGACAAATTTGTTGAGGACAGAGTGGATTTTATGATTGAAGCCGGTTTACTTGAAGAAGTATCTGCATTTTATGATCCTAGGGCAAATTATAAACATGGCTTGTGGCAAGCTxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxAAAGCTAAAGGCCAAGAAATAGAAAAATTAGATAAGGGGAGTCACAAGATCCTGTTAGATGCTGCCATTGAAAGGATGAAGATAAACACTTGCCAACTTGTACGCAAACAAAAACAACGAATCAGTCGACTGAAAACATTCTCTGGTTGGAACCTGCATCAACTTGATTCAACAAAAGCTCTAGAAACATCTGGCTCTGAATCGCTTGAACATTGGGAAATGATGGTAGTCAATATTAGTATGGACGTGGTGAAAAGGTTTTTGTCTGAGCGCACATGTGAAACAAGCTTAAAAGAAAATGTGGAATCCAGAGGTGACTCTATGCATCCAGAGCAAGCACAAGATTTATGGATACATTCCTxxxxxxGGCTTGCAGAGATCAAGTGCTTCAGGGAGCACATGAATGGGAGAAACATAAACTTGGTCGTTCACATCGAAGGCAAATTCTGTGACTTGAAGTAA___TTCAATGACTCGAAAAATGACTTACAAGATTAAATAGTGAGAATCAAGTAGTGGAGGCACAAATTTGGTTTCTTAACATCTGAACAAACTTTGATGAGCTTGTTTGTTAAGGAAAAATGATTGCAAGCTTGATTATAGAAGGAAAAAAACACTCATAGAGAATTTTCTTTTCTGTGATTGCTCATTTCCGAGCGTTTTTGAACTAAAATGCTTTGGATTGTGAGATGGAAAATTGCAAATGTTTGGATGGTTACCGTACTTATCATCATAGCGTTAATTAATGTGATCTTTATGATTCC

Fln Alignment:

sp_v1.1_unigene1920___MQVYEGLDIVTNKVPVEERKGVIHHLLGTVSSKVNFTSSDFRDTAIPIIDSIISRKHLPIIVGGSNSYIQALVSTVDDVMKNGDDDLKTSERPFGICSNAGSVNDLHKRCDNDVQN------------------------------------------TSNQFDQVEDIDLITTNGLHSIDFSQGSADDLHKRCNDDVQNTSNKFD------LIATNGVHPTDFSKGQKGDTADSFRYDCWFLRLDASLPVLDKFVEDRVDFMIEAGLLEEVSAFYDPRANYKHGLWQAxxxxxxxxxxxxxxxxKAKGQEIEKLDKGSHKILLDAAIERMKINTCQLVRKQKQRISRLKTFSGWNLHQLDSTKALETSGSESLEHWEMMVVNISMDVVKRFLSERTCETSLKENVESRGDSMHPEQAQDLWIHSCExRLAEIKCFREHMNGRNINLVVHIEGKFCDLK*FNDSKNDLQD
B8LNK3______________MQVYEGLDIVTNKVPVEERNGVTHHLLGTINSSINFTPKDFRDMAIPIIDSIISRKHLPIVVGGSNSYIQALACS-------GDDDLKTSKRPFSICSNAGNVNDLHKRCDNDVQNISNQFDEVEEIDPIPTNGFCPNDFIRGSANDLHKKCDDDVRNTSNQFDQVEEIDPIATNGLHPNDFSKASANDLHKICDDDVRNTSNQFDQVEEIDLIAPNGLHPNDFSKGQKWDAADSFRYDCCFLWLDASLPVLDKFVEDRVDFMIEAGLLEEVSAFYDPKADYTHGLWQAxxxxxxxxxxxxxxxxD-NGQEIEKLDKGRHKILLDAAIERMKTNTCQLVRRQKLRIDRLKTFFGWNLHRLDSTKALETSGSESLEHWEMMVVNISMDTVKRFLSEHACEASLKENVETRVDSTHPEHAQEFWTHACExRLAEIKCFREHMNGSNINLVVHIGGKFCDLTKFSDSKNNLQD

SNPs (tot: 17)

Position	A %	C %	G %	T %	Probability
751			87	12	0.992554
884	75		25		0.996473
885			25	75	0.996473
902		25		75	0.996473
1057			40	60	1.0
1058			40	60	1.0
1073		16		83	0.994512
1300	90		10		0.90298
1302		10		90	0.90298
1304			90	10	0.90298
1307	90	10			0.90298
1308	90	10			0.90298
1311	90	10			0.90298
1314			90	10	0.90298
1315		10		90	0.90298
1317		10	90		0.90298
1630	12		87		0.992554

SSRs (tot: 1)

Start position	End position	Sequence	Length
193	211	CACAAG CACAAG CACAAG C	19

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain