SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1805

Length: 1587 nt

Origin of reads: Biogeco_1 Biogeco_2 Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene1805 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Allene oxide synthase n=1 Tax=Camellia sinensis RepID=C7BBB6_CAMSI	-	-	51%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	60%
Blast2go	allene oxide synthase	-	-	67%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Cytochrome P450	IPR001128	-	0.0	67%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKD1

Fln msg: Overlapping hits, possible frame ERROR between 489 and 485,

Fln protein: METSSAAAPERSPVMEIPGTYGLSFIGAILDRFEYFKGTDKFFERRVEKYKSTVFRVNMPPGPPFFSYNRVIILLDGKSFPVLYDLSKVDKSYLAGSYMPSLKFTGGYRPTIFMDPSCENLPGxxxRWCLDLLCRNRERNFPEFAKVFDELSVALENEMASSGKASFSDQMDQLLCNFLFRSIAGAYPLAPGPASLGTDGPSCIIKWVASVVGPTISTGVLPKLLDEFTIHLFPLPFFLVSKYYHKLHNFLWMNATSALDVAEKQFGLNREEACNNLVFYIAFNAFGGLRVYFTSMLKYIADAGGKLHTELAEEVRGAVEAHGGLNGKALQSMALVRSTAYEVMRIEPPVPLQYGKAKEDFFVESHGGCYAVNKGDMLCGYQPFATRDPKLFYMAEKFVPRRFMGEEGEKMLKHLL*SNGRETEETSADNKQCAGKDIVGTMGCLFLAHFFVRYDSFAIEKSSSSSVTLTMPTKGFLF*

Protein Length: 480

Fln nts: TCATGCCCTGTCTGTGCTATATAGTCGCTACCTTCCACCACAGCACAGCACAACACATTAGGTTCGCAAAGGGTTCTTTACCATATTTCAGTAAGTTTAGAGACCGGCAAGACG_-_ATGGAGACATCAAGCGCGGCCGCACCAGAGCGGTCTCCTGTAATGGAGATCCCTGGAACTTATGGCCTTTCCTTTATTGGAGCCATCTTGGATCGATTCGAATATTTCAAAGGAACAGATAAATTCTTCGAACGTCGTGTGGAGAAATACAAGAGCACAGTGTTCAGAGTGAATATGCCGCCTGGTCCGCCTTTCTTCTCGTACAATCGAGTCATAATATTGCTAGATGGTAAGAGCTTCCCCGTGCTCTACGATTTGAGTAAGGTGGATAAGAGTTATCTGGCGGGCTCTTACATGCCCAGCCTCAAATTCACCGGCGGATACAGGCCTACCATTTTTATGGACCCATCTTGTGAAAACCTTCCCGGGxxxxxxxxxCGCTGGTGCCTTGATTTACTCTGCAGAAATCGAGAGAGAAATTTTCCAGAGTTTGCCAAGGTGTTCGATGAGCTCAGCGTGGCCCTCGAGAATGAAATGGCGAGTAGTGGGAAGGCAAGTTTCAGTGATCAGATGGATCAATTGCTGTGTAATTTTCTTTTCCGGTCCATTGCCGGCGCTTACCCGTTGGCTCCCGGGCCAGCCAGCCTCGGAACCGATGGTCCTTCCTGTATCATAAAGTGGGTCGCTTCTGTGGTCGGTCCTACCATAAGCACAGGCGTCCTTCCGAAATTGTTGGACGAGTTCACCATCCATTTATTTCCTCTGCCCTTCTTCCTCGTGTCCAAGTATTACCACAAACTCCATAATTTCCTGTGGATGAATGCGACTTCCGCGCTGGATGTGGCCGAAAAGCAGTTCGGCCTGAACAGAGAAGAGGCCTGCAATAATCTTGTGTTTTACATCGCTTTCAACGCCTTCGGGGGACTGCGCGTTTATTTTACCAGTATGCTCAAATATATTGCCGACGCCGGCGGCAAATTGCACACAGAACTGGCCGAAGAGGTCCGCGGAGCTGTTGAAGCACACGGGGGACTCAATGGCAAGGCTCTCCAGAGTATGGCCTTGGTTCGGTCTACTGCGTACGAAGTGATGAGGATAGAGCCTCCTGTGCCATTGCAGTACGGGAAAGCCAAGGAAGATTTCTTCGTCGAATCCCACGGCGGGTGTTATGCGGTGAATAAGGGCGATATGTTGTGCGGGTACCAACCGTTTGCAACCCGGGATCCCAAACTGTTCTACATGGCAGAAAAGTTCGTTCCGCGACGATTCATGGGCGAGGAAGGGGAGAAAATGCTGAAGCACCTGCTGTGATCCAATGGCCGTGAAACAGAAGAAACGTCCGCGGACAACAAGCAGTGTGCGGGGAAGGATATCGTTGGCACTATGGGTTGCCTCTTTCTTGCGCACTTCTTCGTGCGATATGATTCATTTGCCATAGAAAAGTCGTCTTCCTCGTCTGTCACACTTACGATGCCCACTAAGGGCTTTCTCTTCTAA___AATTTTTATCATGTAAGTTGCAAAGTAGATTTCAATTT

Fln Alignment:

sp_v1.1_unigene1805___METSSAAAPERSPVMEIPGTYGLSFIGAILDRFEYF--KGTDKFFERRVEKYKSTVFRVNMPPGPPFFSYNRVIILLDGKSFPVLYDLSKVD-KSYLAGSYMPSLKFTGGYRPTIFMDPSCENLPGxxRWCLDLLCRNRERNFPEFAKVFDELSVALENEMASSGKASFSDQMDQLLCNFLFRSIAGAYPLAPGPASLGTDGPSCIIKWVASVVGPTISTGVLPKLLDEFTIHLFPLPFFLVSKYYHKLHNFLWMNATSALDVAEKQFGLNREEACNNLVFYIAFNAFGGLRVYFTSMLKYIADAGGKLHTELAEEVRGAVEAHGGLNGKALQSMALVRSTAYEVMRIEPPVPLQYGKAKEDFFVESHGGCYAVNKGDMLCGYQPFATRDPKLFYMAEKFVPRRFMGEEGEKMLKHLL*SNGRETEETSADNKQCAGKDIVGTMGCLFLAHFFVRYDSFAIEKSSSS
B8LKD1______________MKVLASMASTAGPVKEIPGSYGPPLLGAIADRFEYFVTEGVDKFFKKRIDKYKSTVLKVNMPPGPLIVSDSRVIMLLDGKSFPVLYDLSKVEKKNVLTGTYMPSTSFTGGYRVAVYLDPSEENHSKxxRFCFEVLGSSRDRYFPEFARAFDELSAAVDKEMASRGKASFGEQIEQLIFNFLCRSLTGADPVTQG---LGTDGPSYVIKWLAPQLAPTLSSGFLPKIVDELTIHSIRLPFWPVSGYYDKLFNFLWTHAAPALDVAEKEFGLKRAEACHDLLFNISFNAFGGMSILFPSIVKYIGNAGNQLQRDLAEEIRGAVRAQGNLNGRALESMALVRSTVYEVLRIDPPVPLQYARAKTDFVVESHDGVYGVKKGELLGGYQPFATKDPKLFDRADEFVPRRFMGPEGEKMLKHVLWSNGRETDETSADNKQCGGKDIVVMVARLFVAHFFLQYDSYTIDQSSSS

SNPs (tot: 57)

Position	A %	C %	G %	T %	Probability
62	12		87		0.992554
77	87		12		0.992554
136	80	20			0.999996
141	90		10		0.90298
147	80		20		0.999996
174		90		10	0.90298
199		80		20	0.999996
226		18		81	0.999994
289		16		83	0.999991
400		14		85	0.999981
434	33		66		1.0
511		15	69		0.999985
556		10	90		0.90298
558	90	10			0.90298
751	85	14			0.993533
753		14		85	0.993533
754		14		85	0.993533
755	14		85		0.993533
756			85	14	0.993533
832		25		75	0.996473
833		25	75		0.996473
834		25	75		0.996473
919	80	20			0.995493
970			75	25	1.0
1049	10	90			0.90298
1058	90		10		0.90298
1067		10		90	0.90298
1078	90		10		0.90298
1085	90		10		0.90298
1095	25	75			0.996473
1099			66	33	0.997452
1148	25		75		0.996473
1159		25	75		0.996473
1173		25	75		0.996473
1175		50		50	1.0
1189			40	60	1.0
1252		11		88	0.923564
1261			11	88	0.923564
1363			33	66	1.0
1375		16		83	0.994512
1456			83	16	0.994512
1467	66		33		1.0
1475	83	16			0.994512
1476		16		83	0.994512
1478		16	83		0.994512
1479		16		83	0.994512
1480	16	66	16		0.988813
1515	20		80		0.995493
1524			80	20	0.995493
1537	20	20	60		0.99255
1540		40	60		1.0
1541		40		60	1.0
1542		40	60		1.0
1543	40	60			1.0
1545		33	66		0.997452
1546		33		66	0.997452
1547		33	66		0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain