SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1648

Length: 1832 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene1648 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	kinesin heavy chain, putative	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	37%
Blast2go	kinesin-related protein	-	-	78%

Source	GOs	Term	Type	e value	Identity
Blast2go	binding	GO:0005488	Molecular Function	0.0	78%

Source	InterPros	Term	Type	e value	Identity
Blast2go	Kinesin, motor domain	IPR001752	-	0.0	78%
Blast2go	Kinesin, motor region, conserved site	IPR019821	-	0.0	78%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACB2

Fln msg: STOP codon was not found. Distance to subject end: 71 aas, No stop codon before M and low homology subject, your sequence is longer than subject: 591 - 509

Fln protein: MHGDLKSPGIIPLAIKDVFNIIQDTPGQEFLLRVSYLEIYNEVINDLLDPTAQNLRVREDSQGTYVEGIKEEVVLSPAHALSLIAAGEEHRHVGSNNFNLLSSRSHTIFTLTIESSVHGSGYDEVTLSQLNLIDLAGSESSKCDTTGLRRKEGSYINKSLLTLGTVIGKLSEGKASHIPYRDSKLTRLLQSSLSGHGHVSLICTITPASSNMEETHNTLKFAQRAKRVEIRAAANRIIDKNSLIKKYQKEISSLKNELDQLKRGILVTPSHEELLTLRQQLEAGQAKMQSRLEEEEQAKAALMGRIQKLTKLILVSTKNTISTNVSDIPTHQHRHSFGEEEKMDAVLESPAACLENETSLNDATSTLPLDLETDIRTGIPKSLSKSVNDHSPYIGSLADSTQAGELFSGTLHGRKPPPTGTTMADQMDLLMEQVKMLAGEVAFSTSSLKRLSEQAAKNPVDIKIQAQIQKMEDEIEEKKRQMRVLERRITGTGEAIANNASPAETQQTIAKLMSQCNEKAFELEIKSADNRILQEQLQAKDSENNELQETILLLRQQLTLSSEKSSQQNNDCSNDFTNRHIDGNLATDESN

Protein Length: 592

Fln nts: GGTGTGAATGGGACTGTGTTTGCTTACGGTGTTACAAGTAGTGGGAAGACTCACACC_-_ATGCATGGTGATCTCAAGTCTCCCGGCATCATACCTTTGGCAATTAAGGATGTTTTTAACATTATTCAAGATACACCTGGTCAAGAGTTTTTGCTTCGTGTTTCTTATCTTGAAATCTACAACGAGGTTATAAATGATTTGCTAGATCCAACAGCACAGAATTTGAGGGTTCGAGAAGATTCCCAGGGTACTTATGTTGAGGGCATTAAGGAGGAAGTTGTCTTGTCCCCTGCACATGCACTTTCATTAATTGCTGCAGGAGAAGAGCATCGCCATGTGGGATCAAACAATTTCAACTTGCTGAGTAGTCGAAGCCACACAATTTTTACTCTGACGATTGAAAGCAGTGTCCATGGCAGTGGATATGACGAAGTCACTTTATCTCAACTTAATCTCATTGATCTGGCTGGATCTGAAAGCTCAAAATGTGACACAACTGGTTTAAGGAGAAAAGAAGGCTCCTACATCAACAAGAGCCTTTTGACACTTGGAACAGTGATAGGAAAGCTAAGTGAGGGAAAGGCTTCTCATATACCTTACCGTGATTCTAAACTCACTCGCCTTTTGCAGTCGTCACTGAGTGGACATGGACATGTATCGCTCATATGCACAATCACACCAGCATCAAGTAACATGGAGGAGACACACAACACATTGAAATTTGCACAAAGAGCCAAACGAGTTGAAATCCGTGCTGCTGCAAATAGGATCATTGATAAAAATTCACTGATCAAGAAGTACCAAAAGGAAATTTCCAGTTTGAAGAATGAGCTTGATCAACTAAAGAGGGGAATTCTTGTGACACCCAGTCACGAGGAGCTTCTTACACTGAGGCAGCAGCTTGAAGCTGGACAAGCGAAAATGCAGTCAAGGTTAGAAGAAGAAGAACAGGCAAAGGCAGCCCTTATGGGTAGAATCCAGAAACTGACAAAGCTAATCCTAGTGTCTACAAAGAATACAATTTCTACAAATGTTTCAGATATTCCTACTCATCAACACAGGCACTCTTTTGGTGAAGAGGAGAAAATGGATGCAGTACTTGAAAGTCCAGCAGCATGTCTAGAAAATGAGACCTCTCTGAATGATGCTACCTCAACGTTGCCTTTGGACTTGGAAACAGATATCAGAACCGGAATTCCAAAATCTTTAAGTAAAAGTGTTAATGACCATTCGCCTTACATTGGCTCACTTGCAGACAGTACACAAGCTGGAGAACTTTTCAGTGGGACTCTTCATGGACGGAAGCCACCTCCTACTGGCACAACTATGGCAGATCAGATGGATCTTCTCATGGAGCAAGTAAAGATGCTGGCTGGTGAGGTTGCTTTTAGTACGAGTTCCCTGAAACGTCTTTCTGAGCAAGCGGCAAAGAATCCTGTGGACATTAAAATTCAAGCTCAAATACAAAAAATGGAGGATGAGATTGAAGAGAAAAAACGCCAAATGAGAGTCCTGGAGCGGCGAATTACTGGAACTGGAGAAGCCATTGCAAATAATGCATCCCCAGCAGAAACACAACAGACAATTGCAAAGTTAATGAGCCAGTGCAATGAGAAAGCTTTTGAGCTAGAGATAAAGTCTGCTGACAACCGTATCCTCCAAGAACAGCTACAAGCAAAGGACTCCGAAAACAATGAGCTGCAGGAGACCATTCTTTTGCTGCGCCAACAGCTTACCTTGTCTTCTGAGAAATCATCTCAGCAAAATAATGATTGTAGCAATGACTTTACAAACAGGCACATTGATGGAAATTTAGCTACCGATGAAAGCAATAG

Fln Alignment:

sp_v1.1_unigene1648___QEFLLRVSYLEIYNEVINDLLDPTAQNLRVREDS-QGTYVEGIKEEVVLSPAHALSLIAAGEEHRHVGSNNFNLLSSRSHTIFTLTIESSVHGSGYDEV---TLSQLNLIDLAGSESSKCDTTGLR--RKEGSYINKSLLTLGTVIGKLSEGKASHIPYRDSKLTRLLQSSLSGHGHVSLICTITPASSNMEETHNTLKFAQRAKRVEIRAAANRIIDKNSLIKKYQKEIS
D5ACB2______________QGFRLWLSFFEIYGGKLYDLLGDR-RKLCMREDARQQVCIVGLQEFEVSDIQCVKEYIEKGNGARSTGSTGANEESSRSHAILQLTIKK--HGEGKELKGGRTVGKLSFIDLAGSERGADTTDNDRQTRMEGAEINKSLLALKECIRALDNDQI-HIPFRGSKLTEVLRDSFVGNSRTVMISCVSPNSGSCEHTLNTLRYADRVKG----------LSKSSNLKKDQSASS

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
1437	80		20		0.995493
1464		40		60	1.0
1527		12		87	0.992554
1566	85		14		0.993533
1568		85	14		0.993533
1570			14	85	0.993533
1571	14	85			0.993533
1573	14			85	0.993533
1574		85	14		0.993533
1577		14		85	0.993533
1582		85	14		0.993533
1583		85		14	0.993533
1585	14			85	0.993533
1587	85		14		0.993533
1590	85		14		0.993533
1737		75	25		0.996473
1738	25	75			0.996473
1739			25	75	0.996473
1741	75		25		0.996473
1743		25		75	0.996473
1744	25			75	0.996473
1756		25		75	0.996473
1759		25		75	0.996473

SSRs (tot: 1)

Start position	End position	Sequence	Length
888	900	TTC TTC TTC TTC T	13

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain