SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1554

Length: 2011 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene1554
AACAAATGGAGTTCATGTAGAAGACAAAAGTAAAGGTTTTCAAGAAGATTTATGTGAGAAACAGACAGTTATTACCAACTGTTCGGTTTCCTTAAAGGAAGAGATTGGATCCCACAAATGGCTTAGAACAGTTTTCAAGAAGCTACTGCTTGAGATTGACAGTTGCCTTCAAGCTGTTGAAGATGTTGTGGTTGCTCCAGGGACATCATCAGTTGATGATCATCCCTTCAGCTGGGCTCCATTCCTTGTAGTTCTCAAAGGTTTGCATGCCATTGCTAAGTTATATGATGATGCAATGGAAGAATTGTTATCGAAACTGCAATTGCGTCGCTCAGCTTTAAACATTCTTATTAAACAGTTGCGGTGGCATGATGACCATTTTTGGCTACTTGACTATGGCTTTCTTCTTGATTTTGAGTCTAAGAAGCGACTTGTTATGGCAATGCTGCCTGAACCACAGGATGACCATGATGAACGACAGGAGGTTATTATTCAGAGATCTCAGCTGCTGACAGAATCATTTGAATTACTGGCTTATGCAGAATCTGAAGTTTTGCAGGGAGGGATTTCTGTGGAGTTTATAAATGAGGAGGCAACTGGCCCAGGTGTCTTGCGGGAATGGTTTTCTCTGATATGCCGTGAAATTTTTAATCCTCAGAATGCACTTTTTTTATCATGCCCAAAAGATCGTCGAAGGTTCTTTCCCAATCCTGCATCTGGTGTGAATCCTGGGCATCTTACCTATTTCAAGTTTTGTGGTCGAGTGATAGCTTTGGCTCTAATGCACAAGGTCCAAGTTGATATAGTTTTTGCTCGCATATTCTTCATGCAATTGACAGGACAACCTGTCTCTTGGGAAGATGTTCAGGATGCAGACCCAGATTTGTATTCTAGTTGCAAGAAGATTTTGGACATGGATGCAGCTTTTCTTGACTCAGATGCTCTAGGGCTCACCTTTGTGACTGAAGTTGAAGAACTGGGGTCCCGGAAGGTAGTGGAGCTTTGCCCAGGTGGTAGGGACCTGGTTGTAGGCAGCAAGAATAGGCATAAGTATGTGAAGCTTCTCGTTCAGCGCCGCTATGTGGCATCAATTACAGAGCAAGTTAAATGCTTTGCTCAGGGATTTTCAGATCTTCTTGTCAATTCCACACATCAACAGTTCCTGCGTGCATTAGAACCTGAAGATATGGACCTCATGTTATATGGATATGATCGAGATATTTGTGTGGATGATTGGAAGGTCCACACAGAGTATCATGATTACTCTGAATCTGATAACACCATAATTTGGTTTTGGCAGGTAGTTGAGGGTATGACAATGGAGCAACGGAGGAAGCTTCTCTTCTTCTCAACATCTGTTACCCACCTGCCTGCGGAGGGATTTTCTGGGCTTTCTTCCAAGTTTCACATACACAGAGCCTATACAGACTTGAGTTGGCTGCCCACGGCACATACTTGTTTTTATCAGCTTGTTCTTCCACCCTACCCTTGCTTTGAGGTGATGTATGCTCGTCTTCATGCTATTACTGAAGGACACATTGCAGAAGGTTTTGGATTTGCATAGAGGAAAATCTCTCATACATTTCAGAATCGATATGGATTAAACAGCTGTATAGCTACGTAGGCAATTTTTGTCCAACTTTTGTTATCCCTGGGTAAATGCAAAAAATTATTCATTGTAGCCTTAATGCTAATGTGGGATGTTTGTGTAAGATATTTAGGCAAAATGATTTGGTTATATCCTAATCACTGTGACTGAAATTTATGAAGGTACAAGGTGAGGGTATATAATTGCAATGATGATGTCTAGTAAGCTTTGAAGCTTAAGTGAAACATCAGAGCTCTGATTGGATAACCATGCTGTATCCATGGAGTTGTAAAAGCAAAGGTGTATGTAAATTTTTCCAGTGCAAGATAGGATAAGTGGCAAGAACATATCAAGTATCTGATGGGAATTTGGAAGGTTCAGATTGCTAATATGTCCTTTTTGAAATGCTTGAATTTCGTTGAA

Ace file of the UniGene sp_v1.1_unigene1554 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	UPL5; UPL5 (UBIQUITIN PROTEIN LIGASE 5); acid-amino acid ligase/ binding / ubiquitin-protein ligase; K10591 E3 ubiquitin-protein ligase NEDD4 [EC:6.3.2.19]	-	-	46%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	74%
Blast2go	ubiquitin-protein upl5	-	-	69%

Source	ECs	Term	Type	e value	Identity
AutoFact	Ubiquitin--protein ligase.	EC:6.3.2.19	-	0.0	46%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5AD16

Fln msg: your sequence is longer than subject: 423 - 118

Fln protein: MEELLSKLQLRRSALNILIKQLRWHDDHFWLLDYGFLLDFESKKRLVMAMLPEPQDDHDERQEVIIQRSQLLTESFELLAYAESEVLQGGISVEFINEEATGPGVLREWFSLICREIFNPQNALFLSCPKDRRRFFPNPASGVNPGHLTYFKFCGRVIALALMHKVQVDIVFARIFFMQLTGQPVSWEDVQDADPDLYSSCKKILDMDAAFLDSDALGLTFVTEVEELGSRKVVELCPGGRDLVVGSKNRHKYVKLLVQRRYVASITEQVKCFAQGFSDLLVNSTHQQFLRALEPEDMDLMLYGYDRDICVDDWKVHTEYHDYSESDNTIIWFWQVVEGMTMEQRRKLLFFSTSVTHLPAEGFSGLSSKFHIHRAYTDLSWLPTAHTCFYQLVLPPYPCFEVMYARLHAITEGHIAEGFGFA*

Protein Length: 424

Fln nts: AACAAATGGAGTTCATGTAGAAGACAAAAGTAAAGGTTTTCAAGAAGATTTATGTGAGAAACAGACAGTTATTACCAACTGTTCGGTTTCCTTAAAGGAAGAGATTGGATCCCACAAATGGCTTAGAACAGTTTTCAAGAAGCTACTGCTTGAGATTGACAGTTGCCTTCAAGCTGTTGAAGATGTTGTGGTTGCTCCAGGGACATCATCAGTTGATGATCATCCCTTCAGCTGGGCTCCATTCCTTGTAGTTCTCAAAGGTTTGCATGCCATTGCTAAGTTATATGATGATGCA_-_ATGGAAGAATTGTTATCGAAACTGCAATTGCGTCGCTCAGCTTTAAACATTCTTATTAAACAGTTGCGGTGGCATGATGACCATTTTTGGCTACTTGACTATGGCTTTCTTCTTGATTTTGAGTCTAAGAAGCGACTTGTTATGGCAATGCTGCCTGAACCACAGGATGACCATGATGAACGACAGGAGGTTATTATTCAGAGATCTCAGCTGCTGACAGAATCATTTGAATTACTGGCTTATGCAGAATCTGAAGTTTTGCAGGGAGGGATTTCTGTGGAGTTTATAAATGAGGAGGCAACTGGCCCAGGTGTCTTGCGGGAATGGTTTTCTCTGATATGCCGTGAAATTTTTAATCCTCAGAATGCACTTTTTTTATCATGCCCAAAAGATCGTCGAAGGTTCTTTCCCAATCCTGCATCTGGTGTGAATCCTGGGCATCTTACCTATTTCAAGTTTTGTGGTCGAGTGATAGCTTTGGCTCTAATGCACAAGGTCCAAGTTGATATAGTTTTTGCTCGCATATTCTTCATGCAATTGACAGGACAACCTGTCTCTTGGGAAGATGTTCAGGATGCAGACCCAGATTTGTATTCTAGTTGCAAGAAGATTTTGGACATGGATGCAGCTTTTCTTGACTCAGATGCTCTAGGGCTCACCTTTGTGACTGAAGTTGAAGAACTGGGGTCCCGGAAGGTAGTGGAGCTTTGCCCAGGTGGTAGGGACCTGGTTGTAGGCAGCAAGAATAGGCATAAGTATGTGAAGCTTCTCGTTCAGCGCCGCTATGTGGCATCAATTACAGAGCAAGTTAAATGCTTTGCTCAGGGATTTTCAGATCTTCTTGTCAATTCCACACATCAACAGTTCCTGCGTGCATTAGAACCTGAAGATATGGACCTCATGTTATATGGATATGATCGAGATATTTGTGTGGATGATTGGAAGGTCCACACAGAGTATCATGATTACTCTGAATCTGATAACACCATAATTTGGTTTTGGCAGGTAGTTGAGGGTATGACAATGGAGCAACGGAGGAAGCTTCTCTTCTTCTCAACATCTGTTACCCACCTGCCTGCGGAGGGATTTTCTGGGCTTTCTTCCAAGTTTCACATACACAGAGCCTATACAGACTTGAGTTGGCTGCCCACGGCACATACTTGTTTTTATCAGCTTGTTCTTCCACCCTACCCTTGCTTTGAGGTGATGTATGCTCGTCTTCATGCTATTACTGAAGGACACATTGCAGAAGGTTTTGGATTTGCATAG___AGGAAAATCTCTCATACATTTCAGAATCGATATGGATTAAACAGCTGTATAGCTACGTAGGCAATTTTTGTCCAACTTTTGTTATCCCTGGGTAAATGCAAAAAATTATTCATTGTAGCCTTAATGCTAATGTGGGATGTTTGTGTAAGATATTTAGGCAAAATGATTTGGTTATATCCTAATCACTGTGACTGAAATTTATGAAGGTACAAGGTGAGGGTATATAATTGCAATGATGATGTCTAGTAAGCTTTGAAGCTTAAGTGAAACATCAGAGCTCTGATTGGATAACCATGCTGTATCCATGGAGTTGTAAAAGCAAAGGTGTATGTAAATTTTTCCAGTGCAAGATAGGATAAGTGGCAAGAACATATCAAGTATCTGATGGGAATTTGGAAGGTTCAGATTGCTAATATGTCCTTTTTGAAATGCTTGAATTTCGTTGAA

Fln Alignment:

sp_v1.1_unigene1554___VELCPGGRDLVVGSKNRHKYVKLLVQRRYVASITEQVKCFAQGFSDLLVNSTHQQFLRALEPEDMDLMLYGYDRDICVDDWKVHTEYHDYSESDNTIIWFWQ
D5AD16______________MELCPGGRDLVVNSKNRHRYVKLLIQCHFVTPVAEQVKCFAQGFSDLLGNSTLQQFLRALEPEDLDLMLYGKGHDLCIQDWKDHTEYHEYTEADEQIIWFWQ

SNPs (tot: 63)

Position	A %	C %	G %	T %	Probability
467	14			85	0.999981
541	87		12		0.992554
548		12		87	0.992554
550	87		12		0.992554
551	87	12			0.992554
552	87	12			0.992554
556	87		12		0.992554
560		12		87	0.992554
563	12		87		0.992554
565	25	62	12		1.0
595		14		85	0.993533
635		16		83	0.994512
636		66	33		1.0
637	33	50		16	1.0
638		16	83		0.994512
640	50	16		33	1.0
642	40		60		1.0
647		40	60		1.0
648	33			66	1.0
649	33		66		1.0
653	66	33			1.0
659		33		66	1.0
947		85		14	0.993533
1041			12	87	0.992554
1042	12			87	0.992554
1045	87		12		0.992554
1047		12		87	0.992554
1048	12			87	0.992554
1049	12	87			0.992554
1050	87		12		0.992554
1056	87		12		0.992554
1058	87	12			0.992554
1074	85		14		0.993533
1163	88		11		0.923564
1306	10			90	0.90298
1308	10		90		0.90298
1309	10		90		0.90298
1329		12		87	0.992554
1330		12		87	0.992554
1331	12			87	0.992554
1396			16	83	0.994512
1397	16	83			0.994512
1398		83	16		0.994512
1399		83		16	0.994512
1401	16	83			0.994512
1402	83	16			0.994512
1409	16	83			0.994512
1410	16			83	0.994512
1413			83	16	0.994512
1415	16	83			0.994512
1416	83	16			0.994512
1418	16			83	0.994512
1419		16		83	0.994512
1437		80	20		0.995493
1440		80	20		0.995493
1445	80	20			0.995493
1448			20	80	0.995493
1449		80	20		0.995493
1450		80		20	0.995493
1452	20			80	0.995493
1454		80	20		0.995493
1455	20	80			0.995493
1456		20		80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain