SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1530

Length: 1427 nt

Origin of reads: Biogeco_1 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene1530 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pseudotzain n=3 Tax=Pinaceae RepID=Q40922_PSEMZ	-	-	64%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	74%
Blast2go	cysteine protease component of protease-inhibitor complex	-	-	81%

Source	GOs	Term	Type	Identity
Blast2go	response to salt stress	GO:0009651	Biological Process	81%
Blast2go	protein binding	GO:0005515	Molecular Function	81%
Blast2go	hydrolase activity	GO:0016787	Molecular Function	81%
Blast2go	vacuole	GO:0005773	Cellular Component	81%
Blast2go	apoplast	GO:0048046	Cellular Component	81%

Source	InterPros	Term	Type	Identity
Blast2go	Granulin	IPR000118	-	81%
Blast2go	Peptidase, cysteine peptidase active site	IPR000169	-	81%
Blast2go	Peptidase C1A, papain C-terminal	IPR000668	-	81%
Blast2go	Peptidase C1A, papain	IPR013128	-	81%
Blast2go	Proteinase inhibitor I29, cathepsin propeptide	IPR013201	-	81%

Full-Lengther Next Prediction

Fln status: C-terminus

Fln database: coniferopsida.fasta

Fln subject: A9NV34

Fln msg: your sequence is shorter than subject: 390 - 452

Fln protein: RRDLGCHSVFKDNLRYIDAQNQRNLSYWLGLNEFADLTHQEFKARYLGTRSDVTRKRKSPSPRYQYNVGDTLPDSVDWRKKGAVAKVKNQAACGSCWAFSAVAAVEGINQIVTNNLITLSEQELVDCDTSYNQGCNGGLMDYAFEFIINNEGIDSDKDYPYKGVDSQCDINRKNAHVVTIDDYEDVPSNNEKALKKAAANQPISVAIEAGGRDFQFYTAGVFTGTCGTNLDHGVTLVGYGWDDGLDYWIVKNSWGSGWGEKGYLRMQRNIDSSNGICGIAMEASYPIKLGPNPPNPGPSPPSPIKPPNKCDDYYLCPESSTCCCIYSYGSYCFGWGCCPLESAVCCNDHYHCCPQEYPICNLDGGTCLKSPNYPIGVAMLKRTPAKFIG*

Protein Length: 391

Fln nts: CGTCGTGACCTTGGCTGTCACTCAGTTTTCAAGGATAACTTGCGTTATATCGATGCGCAGAACCAGAGGAACCTATCATACTGGCTGGGTCTCAATGAATTTGCAGATCTTACACACCAAGAATTCAAGGCGAGATATCTGGGAACCAGATCTGATGTCACAAGAAAGAGGAAGTCTCCGAGCCCTAGATACCAGTACAATGTTGGCGACACTTTGCCAGATAGTGTGGACTGGAGAAAAAAAGGGGCTGTGGCAAAAGTGAAGAATCAAGCCGCTTGCGGAAGTTGTTGGGCATTCTCGGCAGTTGCAGCTGTGGAAGGTATAAACCAAATTGTGACGAATAATTTGATTACTTTGTCTGAACAAGAGTTGGTGGACTGTGATACTTCTTACAACCAGGGATGCAATGGTGGCTTGATGGACTATGCTTTTGAATTCATCATAAACAATGAAGGTATTGACAGCGACAAAGATTATCCTTACAAGGGGGTCGATTCCCAGTGCGACATAAATAGGAAAAATGCACATGTAGTAACCATCGATGACTATGAAGATGTGCCTTCCAATAATGAGAAAGCACTTAAGAAGGCTGCAGCAAACCAACCCATCAGTGTGGCTATTGAAGCTGGTGGTCGTGACTTCCAATTCTATACAGCTGGTGTATTTACAGGCACATGTGGAACCAACCTGGACCATGGTGTGACACTAGTTGGTTATGGGTGGGATGATGGGTTGGACTATTGGATTGTGAAAAACTCATGGGGATCAGGTTGGGGTGAGAAGGGATACTTAAGAATGCAGAGAAACATAGATAGCTCAAATGGTATCTGCGGTATTGCAATGGAAGCATCATACCCAATTAAATTAGGCCCAAATCCTCCAAACCCGGGACCATCTCCACCCTCACCCATCAAGCCACCAAATAAATGTGATGACTACTATTTATGTCCAGAAAGTAGCACTTGCTGCTGTATATATAGCTATGGCAGCTACTGTTTTGGCTGGGGCTGCTGTCCTCTTGAATCTGCTGTCTGCTGTAATGATCACTACCACTGCTGTCCTCAAGAATACCCAATCTGCAATCTTGATGGCGGGACATGCCTAAAGAGTCCAAATTATCCAATTGGAGTTGCGATGCTCAAGCGTACCCCTGCTAAGTTTATTGGGTAG___GTGATGTGGCTGAGGAAGGAGCTGCACATTACACCACTTATTACTAGAGAACGTTGAGTGCCTAATTAATGTGTTCACATCATCAAACTATTGATTCGATCAGTTGAATAAGTTAGGATTTAGTAACGTCTGCAACATAACCCAAGGCATATTTGTCATGGAATCAGAAATCAGTAATTACATTGTAAATACTCGAAGCTACTTTCTTGGATTGTGTAAGTAGCTCAAACAGTAGGCTTAATATCATATATATTTAA

Fln Alignment:

sp_v1.1_unigene1530___SVFKDNLRYIDAQNQRNLSYWLGLNEFADLTHQEFKARYLGTRSDVTRKRKS--PSPRYQYNVGDTLPDSVDWRKKGAVAKVKNQAACGSCWAFSAVAAVEGINQIVTNNLITLSEQELVDCDTSYNQGCNGGLMDYAFEFIINNEGIDSDKDYPYKGVDSQCDINRKNAHVVTIDDYEDVPSXXXXXXXXXXXNQPISVAIEAGGRDFQFYTAGVFTGTCGTNLDHGVTLVGYGWDDGLDYWIVKNSWGSGWGEKGYLRMQRNID-SSNGICGIAMEASYXXXXXXXXXXXXXXXXXXXXXPNKCDDYYLCPESSTCCCIYSYGSYCFGWGCCPLESAVCCNDHYHCCPQEYPICNLDGGTCLKSPNYPIGVAMLKRTPAK
A9NV34______________SVFKDNFLYIHEHNQGNRSYKLGLNQFADLSHEEFKATYLGAKLD-TKKRLSRPPSRRYQYSDGEDLPESIDWREKGAVTSVKDQGSCGSCWAFSTVAAVEGINQIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFEFIINNGGLDSEEDYPYTAYDGSCDSYRKNAHVVTIDDYEDVPENDEKSLKKAAANQPISVAIEASGREFQFYDSGVFTSTCGTQLDHGVTLVGYGSESGTDYWTVKNSWGKSWGEEGFIRLQRNIEVASTGMCGIAMEASYPVKKGANPPNPGPSPPSPIKPPTVCDNYYSCPESNTCCCMYDFGGYCYAWGCCPLDSATCCDDHYSCCPNEYPVCDLDGGTCLKSSKDPFGVKMLKRTPAK

SNPs (tot: 18)

Position	A %	C %	G %	T %	Probability
470	18	81			0.999994
474	18			81	0.999994
475			18	81	0.999994
476		18		81	0.999994
478	18			72	0.999994
481	81			18	0.999994
484		18		81	0.999994
485	81		18		0.999994
487	80	20			0.999996
488	10		20		0.999998
489		12		87	0.992554
855		25		75	0.996473
869	75		25		0.996473
979		25		75	0.996473
1017	25		75		0.996473
1107	25			75	0.996473
1108	75			25	0.996473
1184		66		33	0.997452

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain