SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1288

Length: 1340 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

Ace file of the UniGene sp_v1.1_unigene1288

Annotations

Source	Descriptions	Term	Type	e value	Identity
AutoFact	[TZDR] COG5126 Ca2+-binding protein (EF-Hand superfamily)	-	-	1.0e-17	38%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	0.0	77%
Blast2go	protein	-	-	0.0	68%

Source	InterPros	Term	Type	Identity
Blast2go	Calcium-binding EF-hand	IPR002048	-	68%
Blast2go	EF-hand-like domain	IPR011992	-	68%
Blast2go	EF-Hand 1, calcium-binding site	IPR018247	-	68%
Blast2go	EF-hand	IPR018248	-	68%
Blast2go	EF-HAND 2	IPR018249	-	68%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LKN2

Fln msg: your sequence is longer than subject: 243 - 183

Fln protein: MGFGDSDLVAKIQGFFGSPADHGTVGFILRTLFLYGLLLSLFRYFLPKKFKLWFVAKPFVKIAENSAADREISGNAVGREELRRVFSTFDKNGDGLICKQEMRESFEKLRLCVGEEELLHTIRTVDVNGDGYVDFEEFVTLYESMNCRGGGEEKKETKGADQEDADLAEAFGVFDENGDGLITVEELQAVLKSLGLKEGRAIGECKKMIQKVDKDGDGMVNYLEFKEMMSAGFGRSERNGFS*

Protein Length: 244

Fln nts: ATGGGGACTCACTCCAACAACTTATCCTTCTGGGCGGCTGTGTGAATGAATGTCAAGCGCATGGGCAGCAGCCAAGCCTTGAAATCATGGCCGATAATAGCAGTGAACATTGAAGTTCGGCCGCCAAGAGTCAACTCCAAGGTTACGTTGTGGGCTGTAGTCAATTAGTTCGTTATGTCCGTATTATATAGAGCAATAGTGTGATTCTGACC_-_ATGGGGTTCGGGGATTCAGATTTGGTGGCGAAAATCCAAGGATTTTTCGGCTCTCCGGCGGATCATGGCACGGTGGGCTTCATTCTGAGGACACTCTTTCTGTACGGGCTTTTGCTTTCGCTGTTCAGGTATTTTTTACCCAAGAAGTTTAAGCTCTGGTTCGTCGCCAAGCCCTTTGTGAAAATAGCGGAGAATTCTGCTGCCGACAGGGAAATTTCCGGGAACGCTGTCGGGCGAGAGGAACTGAGGAGGGTTTTCTCGACCTTTGATAAAAACGGCGACGGCCTGATCTGCAAACAGGAGATGAGAGAGTCTTTTGAAAAGCTCAGGCTTTGCGTTGGCGAAGAGGAATTGCTGCACACGATTCGAACCGTGGACGTCAATGGCGACGGGTACGTGGATTTCGAGGAGTTCGTGACGTTGTATGAATCGATGAACTGTAGAGGAGGAGGTGAAGAAAAAAAAGAAACGAAAGGCGCGGATCAGGAAGACGCGGATCTGGCCGAGGCTTTTGGCGTTTTCGACGAGAATGGCGACGGTTTGATAACGGTGGAAGAGCTCCAGGCTGTGCTCAAGTCGCTGGGTCTCAAGGAAGGCCGAGCGATTGGGGAGTGCAAGAAGATGATACAGAAAGTTGATAAAGATGGCGATGGTATGGTCAATTATTTGGAGTTTAAGGAAATGATGAGTGCAGGGTTTGGAAGATCCGAGAGAAATGGATTTTCTTGA___GGGTTTTGGGTCTAGGGTTTTGTAACTTCATACTCTTTATGCAGAAAACTGCATGGTGCTACGTAGAAGGCAGGGCTGTTACTACACCTAATAAAAGCAGATATTCTTCCGCAGCTGGTTGTAAAATTTTAGTTCACGGAACTAACTGTACATTAGCTTAGTTCACTGTCATGACTGTATTTGCTTTGTACTTGGTGGTGAGAGATTTGCTTGGTACTTGGTAGTTTGTGAGAAAACTTGAAAAATATTTGTGTACAATTTTGTGAGAGATGGGGAGACATGCTTGATGGCTTGTGGGAAAACTTGTAAAAGATTGTGTACAAATTTGTGAGATATTACTGAAGGATTTTCTGTTGAACCTGTGTGTGGGTATTATTAATAAAAACGGAGTTTCATGTT

Fln Alignment:

sp_v1.1_unigene1288___IAENSAADREISGNAVGREELRRVFSTFDKNGDGLICKQEMRESFEKLRLCVGEEELLHTIRTVDVNGDGYVDFEEFVTLYESMNCRXXXXXXXXXXXADQEDADLAEAFGVFDENGDGLITVEELQAVLKSLGLKEGRAIGECKKMIQKVDKDGDGMVNYLEFKEMMSAGFGRS
B8LKN2______________MADNSAEG--VSNNAVGRDELRRVFAIFDKNGDGLISKQEMRESFDKLRLCIGEEELASTIRTVDVNGDGYVDFDEFVTLYESMSGKRGEGGDAK---AEHEDADLAEAFGVFDENGDGLITVEELQSVLKSLCFEEGRTIGDCKKMIQKVDKDGDGMVNYMEFKEMMSAGFGRN

SNPs (tot: 40)

Position	A %	C %	G %	T %	Probability
21	80		20		0.995493
22		80	20		0.995493
23			20	80	0.995493
24			20	80	0.995493
26		20		80	0.995493
54			16	83	0.994512
55		83	16		0.994512
137			14	85	0.993533
195		85	14		0.993533
215		14		85	0.993533
235	14			85	0.993533
240			85	14	0.993533
315	11		88		0.923564
322		88		11	0.923564
328	22	77			0.999999
389		20		80	0.999996
414		18		81	0.999994
420		81	18		0.999994
468		12		87	0.992554
480		25		75	1.0
576			88	11	0.923564
597	11			88	0.923564
606			87	12	0.992554
609		25		75	1.0
634	11			88	0.923564
638	88		11		0.923564
639			11	88	0.923564
651	12	87			0.992554
654			14	85	0.993533
655	83		16		0.994512
969		12		87	0.992554
1014		12		87	0.992554
1030		41		58	1.0
1033	45		54		1.0
1043			45	54	1.0
1053		18		81	0.999994
1111		26	73		1.0
1189			18	81	0.999994
1194	18		81		0.999994
1202	18			81	0.999994

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain