SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1256

Length: 2257 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene1256
CTGATGCGAGGTCAACTATTAAGCTATTTGAAATGCTTTGATTGGTCAGAAGCAGAAAAGACTGGCCGTATTCTGCCTTGTGAAGGTGTGGATAAGGACTATGATGCGGCTAATAGAACTGTGAGAGAAATTGAAATAGAGCTAGAAAAACACTTGGAAGTGCAGCGGAAGTATTTTGGAAGTGATATGATAAATTATGTTACTGTTGGAAAAGAGCACTATCAAATAGAGATACCTGAAAGTCTGCTTGCAAAAGTTCCTCAGGATTATGAAGCTCGGTCATCAAGAAAGGGTTTTCGCAGGTTTTGGACACCAAAGGTTAAACACCTATTACAAGACCTCGCTGATGCACAAGGACAACGAGAAGCATCCTTGAGAGGAATATTGCAAGGTTTGATTAGACAATTTTGCGAGCACCATTGCATATGGCATGGGATTGTTAATGTGGTTGCAGAGATGGACGTGTTAATAAGTCTAGCACTTGCTAGTAATTACTTTGTAGGACCTACATGCAGGCCATCTATCAAAGACACCACTAAGCTATTGAGTGATGAAGCTGACACCCCGTACTTACGTGCTAAAGAGTTAGGACATCCAGTTTTATCTAGCCTAAGTGGAAGTGGGTGCTTTGTTCCGAATGATGTGAATATAGGTGGGCACAGAAATGCTTGTTTCATGCTTCTTACAGGACCCAACATGGGTGGAAAATCAACTCTTCTTCGGCAAGTATGCTTGGCAGTTGTCTTGGCACAGCTTGGGGCAGATGTTCCTGCTGAAGAGTTTGAATTTTCACCAGTTGACCGCATATTTGTTCGTATGGGAGCAAAGGACCATATCATGGCTGGGCAAAGTACTTTTCTTGTTGAACTCTTAGAGACGGCATCTATGCTGTCATCTGCAACACGAGATTCACTGGTAGTATTAGATGAACTTGGACGTGGAACTGCAACTTCTGATGGACAGGCAATTGCGCATGCAGTGCTAGAACATCTTGCCCATGAAACTGGATGTCGTGGCTTGTTTTCTACTCACTATCATCACCTAGCTACGGACTTCGCGAGTGATCCTTCCGTTGGTTTGTACCATATGGCCTGTAAGGTTGGCACACAAATTGGAGGTTTGGAGGAGGTGACATTTCTTTATAAACTGGCTCAGGGTACATGCCCGAAGAGTTATGGTGTGAATGTTGCCCGACTTGCAGGAATGCCAGACACCATTCTTGAAAGGGCTGCTAAAAAATCAGCTGAGTTTGAGAGTCAATATGGACAGAGGAGCATCAAATGTGATGGAAGACCTTTGGCATCCGTAAAACTAAAAGAGAGGAACCTCATCCAAGAGGTTTTGAACATTGTAACGGGATTGCACTCCTCGGGAACTACAGCAGGTAGTGACATGAGATGTTTGGTTGAAGTGTGGCACAGAGTAGCGTCACATTCTTTATCGGATTGTTCTCATTCTAAGCACTCCTTGGTAACAAGGCTTTTTGATTGCCACCCTCTTCATAGCACAATTCCACCTGGGATGTAAATAATTGTGAATGAAGCATTTACTTGGCATGTCAAAGTGGTTGATGCATGTAAAAGTTGGTTGTTGAAATAACCTGTGGCTTGATAGAGTGTATGCAATGGATATGCATTCACTGGACTTGTATTATCTGCATGCCCCAGATTGTTTACTCCAGGGAGTTTTTTGCTAATGAAGACTGAATCACAATGAAGTAGCATATGTGCTGGTAGGGTTTTGAAGCAGTTCCTGTGCTGAAACAGGATTTAAGTTTCGAGGAAGCATTTTTGCACATGCTCATGCAGGACTTGTTGAAATAATGCCATCTGCTGTGAAGTTTTGAAATTAATTTGTCAAATATTATTTCATTCGAGTTCCAGGCAGTGTTAGCATTATGGCCATCCCACACTAGTATATTGGGCCTGAATATATCTGATAAAAATCTGTTTACAGAATGACATGTGGTTACATCGGTTACATCCATCATGTCTTGTACTATTTAGATATTAATGATTGTTGGGCATTGTATCTGCTTTTTGTAATCAAAGTTACACAGCAAGGGCATTTTGAATTTCTTTGGAGAACTTTTGCTACAAGGCATACAGGGGCTTCATGCCTAATTGCATATGAATTTAATTGTTGTGTTAAAGCGAGGACCATGCACTGTTCAAGTCTGTTTGCAGATCTTGGTGGCAGTTTTCCACATTGAAAGGGAACATGAATGAACGGTAGACTAGAATGACCACCAGATTTC

Ace file of the UniGene sp_v1.1_unigene1256

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	ATP binding protein, putative; K08737 DNA mismatch repair protein MSH6	-	-	57%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	81%
Blast2go	atp binding	-	-	71%

Source	GOs	Term	Type	e value	Identity
Blast2go	damaged DNA binding	GO:0003684	Molecular Function	0.0	71%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: D5A8M4

Fln msg: Overlapping hits, possible frame ERROR between 1688 and 1663, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 48 - 73

Fln protein: MQWICIHWTCIICMxxxxxxxxxxLLMKTESQ*SSICAGRVLKQFLC*

Protein Length: 49

Fln nts: CTGATGCGAGGTCAACTATTAAGCTATTTGAAATGCTTTGATTGGTCAGAAGCAGAAAAGACTGGCCGTATTCTGCCTTGTGAAGGTGTGGATAAGGACTATGATGCGGCTAATAGAACTGTGAGAGAAATTGAAATAGAGCTAGAAAAACACTTGGAAGTGCAGCGGAAGTATTTTGGAAGTGATATGATAAATTATGTTACTGTTGGAAAAGAGCACTATCAAATAGAGATACCTGAAAGTCTGCTTGCAAAAGTTCCTCAGGATTATGAAGCTCGGTCATCAAGAAAGGGTTTTCGCAGGTTTTGGACACCAAAGGTTAAACACCTATTACAAGACCTCGCTGATGCACAAGGACAACGAGAAGCATCCTTGAGAGGAATATTGCAAGGTTTGATTAGACAATTTTGCGAGCACCATTGCATATGGCATGGGATTGTTAATGTGGTTGCAGAGATGGACGTGTTAATAAGTCTAGCACTTGCTAGTAATTACTTTGTAGGACCTACATGCAGGCCATCTATCAAAGACACCACTAAGCTATTGAGTGATGAAGCTGACACCCCGTACTTACGTGCTAAAGAGTTAGGACATCCAGTTTTATCTAGCCTAAGTGGAAGTGGGTGCTTTGTTCCGAATGATGTGAATATAGGTGGGCACAGAAATGCTTGTTTCATGCTTCTTACAGGACCCAACATGGGTGGAAAATCAACTCTTCTTCGGCAAGTATGCTTGGCAGTTGTCTTGGCACAGCTTGGGGCAGATGTTCCTGCTGAAGAGTTTGAATTTTCACCAGTTGACCGCATATTTGTTCGTATGGGAGCAAAGGACCATATCATGGCTGGGCAAAGTACTTTTCTTGTTGAACTCTTAGAGACGGCATCTATGCTGTCATCTGCAACACGAGATTCACTGGTAGTATTAGATGAACTTGGACGTGGAACTGCAACTTCTGATGGACAGGCAATTGCGCATGCAGTGCTAGAACATCTTGCCCATGAAACTGGATGTCGTGGCTTGTTTTCTACTCACTATCATCACCTAGCTACGGACTTCGCGAGTGATCCTTCCGTTGGTTTGTACCATATGGCCTGTAAGGTTGGCACACAAATTGGAGGTTTGGAGGAGGTGACATTTCTTTATAAACTGGCTCAGGGTACATGCCCGAAGAGTTATGGTGTGAATGTTGCCCGACTTGCAGGAATGCCAGACACCATTCTTGAAAGGGCTGCTAAAAAATCAGCTGAGTTTGAGAGTCAATATGGACAGAGGAGCATCAAATGTGATGGAAGACCTTTGGCATCCGTAAAACTAAAAGAGAGGAACCTCATCCAAGAGGTTTTGAACATTGTAACGGGATTGCACTCCTCGGGAACTACAGCAGGTAGTGACATGAGATGTTTGGTTGAAGTGTGGCACAGAGTAGCGTCACATTCTTTATCGGATTGTTCTCATTCTAAGCACTCCTTGGTAACAAGGCTTTTTGATTGCCACCCTCTTCATAGCACAATTCCACCTGGGATGTAAATAATTGTGAATGAAGCATTTACTTGGCATGTCAAAGTGGTTGATGCATGTAAAAGTTGGTTGTTGAAATAACCTGTGGCTTGATAGAGTGT_-_ATGCAATGGATATGCATTCACTGGACTTGTATTATCTGCATGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTTGCTAATGAAGACTGAATCACAATGAAGTAGCATATGTGCTGGTAGGGTTTTGAAGCAGTTCCTGTGCTGA___AACAGGATTTAAGTTTCGAGGAAGCATTTTTGCACATGCTCATGCAGGACTTGTTGAAATAATGCCATCTGCTGTGAAGTTTTGAAATTAATTTGTCAAATATTATTTCATTCGAGTTCCAGGCAGTGTTAGCATTATGGCCATCCCACACTAGTATATTGGGCCTGAATATATCTGATAAAAATCTGTTTACAGAATGACATGTGGTTACATCGGTTACATCCATCATGTCTTGTACTATTTAGATATTAATGATTGTTGGGCATTGTATCTGCTTTTTGTAATCAAAGTTACACAGCAAGGGCATTTTGAATTTCTTTGGAGAACTTTTGCTACAAGGCATACAGGGGCTTCATGCCTAATTGCATATGAATTTAATTGTTGTGTTAAAGCGAGGACCATGCACTGTTCAAGTCTGTTTGCAGATCTTGGTGGCAGTTTTCCACATTGAAAGGGAACATGAATGAACGGTAGACTAGAATGACCACCAGATTTC

Fln Alignment:

sp_v1.1_unigene1256___LIECMQWICIHWTCIICMxxxxxxxxxLLMKTESQ*SSICAGRVLKQFLC*NRI
D5A8M4______________LVECMQWVCIHWMCIICMxxxxxxxxxLLMKPELRSSSTHAGRVLKQFLCWNRM

SNPs (tot: 60)

Position	A %	C %	G %	T %	Probability
292	75		25		0.996473
293	75		25		0.996473
452		14		85	0.993533
500	10			90	0.90298
503			10	90	0.90298
504	90		10		0.90298
678		85	14		0.993533
686	14			85	0.993533
688	85		14		0.993533
689		85	14		0.993533
690	85		14		0.993533
777		20		80	0.995493
956		80	20		0.995493
957	20			80	0.995493
1215		80	20		0.995493
1216	80		20		0.995493
1217		80	20		0.995493
1286	14		85		0.993533
1289	71		14		0.984337
1290			14	85	0.993533
1330		77	22		0.999999
1333	77		22		0.999999
1335	11	88			0.923564
1337	77			22	0.999999
1338	77		11	11	0.968132
1340	77	11			0.968132
1341			88	11	0.923564
1342		11	88		0.923564
1343		10		90	0.90298
1344	10		20	70	0.999998
1345		10		90	0.90298
1346		20		80	0.999996
1349	90			10	0.90298
1351	90	10			0.90298
1356	90		10		0.90298
1357	90	10			0.90298
1358		90		10	0.90298
1360			90	10	0.90298
1361		10	90		0.90298
1363		10		90	0.90298
1461			14	85	1.0
1462	76			23	1.0
1463	76	14	9		1.0
1466	76			23	1.0
1467		76	23		1.0
1468	14			85	1.0
1470	9	90			0.999872
1471		9		90	0.999872
1473		9	90		0.999872
1474	9		90		0.999872
1476	85		14		1.0
1477	85			15	1.0
1480	90			10	0.999898
1481		15	85		1.0
1482	10		85	5	0.999793
1495		79	20		1.0
1717	12			87	0.992554
1718			87	12	0.992554
1750		57		42	1.0
1970	66		33		1.0

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain