SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene1032

Length: 2059 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene1032
CGTCGTGACCGTCGTGACCTTGGCTGTCACTCAGTTTTATCTGACCATCTCCCTGAATTCTAGCTTTAGGGCATCATTCCGAGCTTGCAGCAACACGCGTCCTTGAATTTCGTCAATTTGTTCGTCTCTCGAGATTTTCCCTCATTTCTGAAATTACAGCCGTTTTCATGTTTCGAGAGTCATTGGTTGCTGCAGCAATAGAGCATTGCTGTGAATATACAGTGACATTATTTCTCAAGGCAAGCAGGGTGGAGTATCATTCCACAGCATTAGGTTACGATTGTTTGAGCTTCTACAATGAAAAAGACTAACCAGGGACAGTTACATTATGTATTTCTAGTTGCAGGAATGGTCTGCCTTCAAATCTTTGCTCCCAAATGTTCTGCAGCAAAAACTCAGGGAATGTTGGATAGTATTATGGGTTTCCTTCGAGGACGCCCCACTATACAAGCAACAGAGCAGCAACTACGACACCAGAAACAGAATTCTTCTTTTCCAGTACAGAATCCGGAAGAAGTAGTTAAAATGGTTCAAAAAAGTATCAATGATTCTCGAAGACAGCTTACCTATTTGACCTGTGGAACCGGGAATCCCATCGATGACTGTTGGCGCTGTGACCCAAACTGGCAAATGAACAGGCAAAAACTTGCAGATTGTGCTATTGGCTTTGGCCGAGAGGCCATTGGAGGGAAGAATGGTCAATACTATGTGGTAACTGATCCCAGTGACCATGATCCTGTGAATCCCACCCCCGGTACTCTTCGCTACGCCGTTATTCAAAACGAGCCGTTATGGATTATATTCGAGAGCGACATGATCATCCAGCTCAAGGAAGAGCTCCTCATGAACAGCTTCAAGACAATCGATGGCAGAGGCGTTGATGTCCACATTGCTCATGGTGCCTGCATTACGATACAGTATGTGACCAACATCATCATTCATGGCGTTAGCATCCACGACTGCATTCAGACGGGCAATGCTATGGTGAGGGATTCTCCAGAACATTATGGCTGGAGAACGTTGGCAGATGGGGATGGCATTTCAATTTTTGGAGGACGCTACATCTGGATAGACCGCTGTGCTTTTTCCAGCTGTAACCACGGCCTAATAGATGCAATCATGGGATCCACTGCCATCACCATATCAAACAATTACTTCACTCAGCATGACCTGGTCATGCTTCTTGGTCACAGCGACAGCTATGTGCAAGATGTTATAATGCAAGTAACGATCGCCTACAATTACTTCGGGGAAGGGCTTGTCCAAAGGATGCCTAGGTGTCGACATGGGTACTTTCACGTGGTGAACAACCAGTATGTGCACTGGGAAATGTATGCAATCGGTGGGAGCGCTAACCCAACAATTAACAGTGAAGGCAACATATTCATAGCGTCCGATGACGCCAACGTTAAGCAGGTAACTAAACGGGTTACTGCAGGAGACGAGGGAGAGGAGGCAAACGAGGTATGGCAGGACTGGAACTGGAGATCGGTAGGAGACCAAATGCTGAACGGTGCATATTTTGTCCCTTCAGGCTCCATAGCGGACTCTAGCTATACCCTAGCCTCCAGTCTGGGAGCAAAGCCAGCGGTCTTTGTCGAAACCCTTACCGAAGAGGCAGGCGTCTTACAAGGCAGATCCCAAACAAGTAGCCGCAACGGCAACAGTCATCTGTCAGTTTCAGCTGCACCGAGTTCAATCTTGTCAGCGTCTCACCGTCATCAGATAATATTCTTCTTCATGTGCACTGCGTGGCTAGTATTGACAGCGTTGAACGTTTGAAGGTAGTGTTTGTCTTCTTCCTATGGACAATCGTTTAATACGAGCTGGTATTGACAGCGTTGAACGTTTGAAGGTAGTGTTTGTCTTCTTCCTCTGGACAATCGTTTAATACGAGCTGGAAGCCCAATTATCTCTGGTCTTGATGTCAGAACGTAGGTTCCGTGGGCCAATGTACGTTCTGTGAGCTGTGCCATTCCATGGATGATGTTCCAGTTTGTATCAAGTTTTTGTTATAAAAATTATTTCAAAAATATTGTATAGATTTTAGATATTAATA

Ace file of the UniGene sp_v1.1_unigene1032 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Pectate lyase precursor, putative (EC:4.2.2.2)	-	-	70%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	76%
Blast2go	pectate lyase	-	-	81%

Source	InterPros	Term	Type	Identity
Blast2go	Pectate lyase/Amb allergen	IPR002022	-	81%
Blast2go	Pectin lyase fold/virulence factor	IPR011050	-	81%
Blast2go	Pectin lyase fold	IPR012334	-	81%
Blast2go	AmbAllergen	IPR018082	-	81%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: B8LS41

Fln msg:

Fln protein: MKKTNQGQLHYVFLVAGMVCLQIFAPKCSAAKTQGMLDSIMGFLRGRPTIQATEQQLRHQKQNSSFPVQNPEEVVKMVQKSINDSRRQLTYLTCGTGNPIDDCWRCDPNWQMNRQKLADCAIGFGREAIGGKNGQYYVVTDPSDHDPVNPTPGTLRYAVIQNEPLWIIFESDMIIQLKEELLMNSFKTIDGRGVDVHIAHGACITIQYVTNIIIHGVSIHDCIQTGNAMVRDSPEHYGWRTLADGDGISIFGGRYIWIDRCAFSSCNHGLIDAIMGSTAITISNNYFTQHDLVMLLGHSDSYVQDVIMQVTIAYNYFGEGLVQRMPRCRHGYFHVVNNQYVHWEMYAIGGSANPTINSEGNIFIASDDANVKQVTKRVTAGDEGEEANEVWQDWNWRSVGDQMLNGAYFVPSGSIADSSYTLASSLGAKPAVFVETLTEEAGVLQGRSQTSSRNGNSHLSVSAAPSSILSASHRHQIIFFFMCTAWLVLTALNV*

Protein Length: 496

Fln nts: CGTCGTGACCGTCGTGACCTTGGCTGTCACTCAGTTTTATCTGACCATCTCCCTGAATTCTAGCTTTAGGGCATCATTCCGAGCTTGCAGCAACACGCGTCCTTGAATTTCGTCAATTTGTTCGTCTCTCGAGATTTTCCCTCATTTCTGAAATTACAGCCGTTTTCATGTTTCGAGAGTCATTGGTTGCTGCAGCAATAGAGCATTGCTGTGAATATACAGTGACATTATTTCTCAAGGCAAGCAGGGTGGAGTATCATTCCACAGCATTAGGTTACGATTGTTTGAGCTTCTACA_-_ATGAAAAAGACTAACCAGGGACAGTTACATTATGTATTTCTAGTTGCAGGAATGGTCTGCCTTCAAATCTTTGCTCCCAAATGTTCTGCAGCAAAAACTCAGGGAATGTTGGATAGTATTATGGGTTTCCTTCGAGGACGCCCCACTATACAAGCAACAGAGCAGCAACTACGACACCAGAAACAGAATTCTTCTTTTCCAGTACAGAATCCGGAAGAAGTAGTTAAAATGGTTCAAAAAAGTATCAATGATTCTCGAAGACAGCTTACCTATTTGACCTGTGGAACCGGGAATCCCATCGATGACTGTTGGCGCTGTGACCCAAACTGGCAAATGAACAGGCAAAAACTTGCAGATTGTGCTATTGGCTTTGGCCGAGAGGCCATTGGAGGGAAGAATGGTCAATACTATGTGGTAACTGATCCCAGTGACCATGATCCTGTGAATCCCACCCCCGGTACTCTTCGCTACGCCGTTATTCAAAACGAGCCGTTATGGATTATATTCGAGAGCGACATGATCATCCAGCTCAAGGAAGAGCTCCTCATGAACAGCTTCAAGACAATCGATGGCAGAGGCGTTGATGTCCACATTGCTCATGGTGCCTGCATTACGATACAGTATGTGACCAACATCATCATTCATGGCGTTAGCATCCACGACTGCATTCAGACGGGCAATGCTATGGTGAGGGATTCTCCAGAACATTATGGCTGGAGAACGTTGGCAGATGGGGATGGCATTTCAATTTTTGGAGGACGCTACATCTGGATAGACCGCTGTGCTTTTTCCAGCTGTAACCACGGCCTAATAGATGCAATCATGGGATCCACTGCCATCACCATATCAAACAATTACTTCACTCAGCATGACCTGGTCATGCTTCTTGGTCACAGCGACAGCTATGTGCAAGATGTTATAATGCAAGTAACGATCGCCTACAATTACTTCGGGGAAGGGCTTGTCCAAAGGATGCCTAGGTGTCGACATGGGTACTTTCACGTGGTGAACAACCAGTATGTGCACTGGGAAATGTATGCAATCGGTGGGAGCGCTAACCCAACAATTAACAGTGAAGGCAACATATTCATAGCGTCCGATGACGCCAACGTTAAGCAGGTAACTAAACGGGTTACTGCAGGAGACGAGGGAGAGGAGGCAAACGAGGTATGGCAGGACTGGAACTGGAGATCGGTAGGAGACCAAATGCTGAACGGTGCATATTTTGTCCCTTCAGGCTCCATAGCGGACTCTAGCTATACCCTAGCCTCCAGTCTGGGAGCAAAGCCAGCGGTCTTTGTCGAAACCCTTACCGAAGAGGCAGGCGTCTTACAAGGCAGATCCCAAACAAGTAGCCGCAACGGCAACAGTCATCTGTCAGTTTCAGCTGCACCGAGTTCAATCTTGTCAGCGTCTCACCGTCATCAGATAATATTCTTCTTCATGTGCACTGCGTGGCTAGTATTGACAGCGTTGAACGTTTGA___AGGTAGTGTTTGTCTTCTTCCTATGGACAATCGTTTAATACGAGCTGGTATTGACAGCGTTGAACGTTTGAAGGTAGTGTTTGTCTTCTTCCTCTGGACAATCGTTTAATACGAGCTGGAAGCCCAATTATCTCTGGTCTTGATGTCAGAACGTAGGTTCCGTGGGCCAATGTACGTTCTGTGAGCTGTGCCATTCCATGGATGATGTTCCAGTTTGTATCAAGTTTTTGTTATAAAAATTATTTCAAAAATATTGTATAGATTTTAGATATTAATA

Fln Alignment:

sp_v1.1_unigene1032___QGQLHYVFLVAGMVCLQIFAPKCSAAKTQGMLDSIMGFLRGRPTIQATEQQLRHQKQNSSFPVQNPEEVVKMVQKSINDSRRQLTYLTCGTGNPIDDCWRCDPNWQMNRQKLADCAIGFGREAIGGKNGQYYVVTDPSDHDPVNPTPGTLRYAVIQNEPLWIIFESDMIIQLKEELLMNSFKTIDGRGVDVHIAHGACITIQYVTNIIIHGVSIHDCIQTGNAMVRDSPEHYGWRTLADGDGISIFGGRYIWIDRCAFSSCNHGLIDAIMGSTAITISNNYFTQHDLVMLLGHSDSYVQDVIMQVTIAYNYFGEGLVQRMPRCRHGYFHVVNNQYVHWEMYAIGGSANPTINSEGNIFIASDDANVKQVTKRVTAGDEGEEANEVWQDWNWRSVGDQMLNGAYFVPSGSIADSSYTLASSLGAKPAVFVETLTEEAGVLQGRSQTSSRNGNS-HLSVSAAPSSILSASHRHQIIFFFMCTAWLVLTA
B8LS41______________QSQLCYLILFAELICLQILAPKCSAEKTQGMMDSFMGFLRGRPTIQVTEQT---QQQNSSFPVEAPDEVVKMVQKSINDSRRQLSYLSCGTGNPIDDCWRCEPNWQMNRQRLADCAIGFGRDAIGGKNGRYYVVTDSSDEDAVNPKPGTLRHAVIQDEPLWIIFQSDMVIQLKEELMMNSFKTIDGRGADVHIAHGACITIQFVTNIIIHGVSIHDCFQAGNAMVRNTPEHYGWRTVSDGDGISIFGGRYIWIDHCSLSNCKDGLIDAIMGSTAITISNNYFTHHDKVMLLGHSDSYVGDAIMQVTIAFNYFGEGLMQRMPRCRHGYFHIVNNHYSHWEMYAIGGSANPTINSEGNRFIAPDNANAKQVTKRLNAVD------EEWMRWNWRSAGDLMVNGAFFVPSGAGTGNNYALASSVGAKSAFLVKTITEDAGVLQSRTHSSRSNDNTGRMSVSAAPMSILSAAHSHQIIYFFLCALCYVLTA

SNPs (tot: 16)

Position	A %	C %	G %	T %	Probability
1783	85			14	0.993533
1877	75		25		1.0
1972		12		87	0.992554
1988	87		12		0.992554
2032			25	75	1.0
2034	62	25	12		1.0
2035	12	12	62	12	0.962769
2036		28	14		1.0
2041		85	14		0.993533
2043	14		85		0.993533
2044		85		14	0.993533
2045		85		14	0.993533
2046	85		14		0.993533
2050	16			83	0.994512
2054		16	83		0.994512
2056	16	83			0.994512

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain