SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene819

Length: 3079 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

>sp_v1.1_unigene819
GTGTGCAACCTCTCTGCGCGAGTTCTTCAGACATCTTCGTGAAATTGAGAGGCCAAATCCTGAGAGGGAAGATGATGGCATCAACAGGCAAAATGGGGAAAGAGCAGGAAGGCGTCTACCTGTACCAGATGGAGCTCATCTTGTTGGGGGAAATGGTGAGGGTGGTGGAGCTGGCCAGGAAAACATTGGTGCAGGGCAATTGATCAGGAGAAATGCACAAAATGTAGCGGCTCGTGTGGAAATGCAAGCGGCACGACTTGAGGCACATGTTGAGCAAATGTTTGATGGTGGAGATGATGCGGATGGTATTGAGGATGTGCCATTTGATGAACTTGTGGGGATGCAAGGTCCTGTGTTTCATTTGATTGAAAATGCAATAACGGTGCTGGCTAGTAACATGATATTTCTTGGTGTTGTAATCTTTCTACCTTTCCAACTAGGTCGGATACTTCTTTCCATTGCATCTCAACTTGCTGTCACCACATCTAGTGCATTACTTACAGCAGGAATGTCTAGTTCTTCTGGGTCAGATTTGGCTCTCTCTGACATTACTTTAAAGAATAGTCTCGAGTCAGCTGCAAATATGTCAGCAAGTGCCCTGACCAAGGAGCTACTTCAAGGGAATATGAGCACTGGTGTGCTTCACTCTGATGGTGGTTCAAATGTTACTAAATCTGGGGGATCTGTGGAAGTATTGACAGAAGCCTTGCTTGCTTCGTTGCGGGTTTCTGATGCTGCAACTCTTTCTATCGGTTACATGGTTATTTTGCTTTTTGTTTTATGTTATCTTGGCTTAATAGCCTTGATACGCTACAGCAGAGGTGAGCCTTTGACAGTGGGTAGGATCTATGGTATAGCTTCGGTGGCAGAGGCTGCTCCTTCTCTTGCAAGGTCGTTGCTGTCTGGAGTAAAATACTTTTTCACCATGGTGAAGGTTGCTTTTCTTTTGCTAGTGGAGATTGGGTTTTTCTCCTTTATTATGTGGTTGGTGGCTTGATGTGTGCACCATTAGCATGCTTGGAACAACATTGTCTCAGAGGGTGGGCTTTTTCTGGGAGTCACCTTTAACAAGTTCATTCCTTCACTGGCTTGTTGGGATTGTGTATATGTTACAAATAAGCATCTTTGTCAGCCTTCTTCGAGAGGTACTTCGCCCTGGTGTCCTATACTTTCTCAGAGATCCTGCTGATCCAAATTATAATCCATTCCGAGATCTCATTGATGATCCGATACATAAACATGCACGAAGGGTCTTGCTGTCTGTTGTGGTTTATGGAAGCTTGATTGTCTTGCTGATATTCCTACCAGTCAAACTTGCAATGTTTTTGGCACCTTCACTATACCCTTTGGATACATCGATGTCAGATCCATTTACAGAGATCCCAGCAGACATGCTTTTGTTTCATATCTGTATTCCATTTGCCATTGATCACTTCAGGCCACGGGCAACTTTTAAGAATGTTCTTTTCCACTGGTTTTCTGTTACTGGTTGGATGCTTGGATTGTCTGACTTCCTCTTGCCAAACCCCGAGGACGGGAATGGTCAAGATGGTTTGAATGGTCATCAAAGAATACAGGGAAGGTTACAAGATGTTCGTCGCCATGGTGTCCAAGAACAGAATCGCCATAGATTAGCACTTCCTGCTGCTGGGGTTGCTGGGAGGGTGGTGCATGAAAATGAGGGTGCGACGTTTGAAGAGAATGATGGAGATGAACAAGATGATTCAGAAGAATATAAATTTGCTATCTGCATTGTATTGCTGCTATTTGGGGCATGGTTAACCATGCTCTGCTTCAACTCAGCAATGTTTGTGATACCTATTTCGTTAGGGCGGGCAATGTTTGCTTCAATTCCACGGCTTCCAATTACCCATGGCATTAAGTGCAACGATCTTTATGCCTTCAATATCGGATGTTATGTCATTTGGGCTGCGGCAGCTGGAGTTGGATATTTAGTAGACTATTTGAGAGTTCACAGCTTCAGAGTTCTTTTCATGCAGTTCATAAAATGGTCTTTAATAGTTCTGAAAAGTTGTATTCTTCTATCATTATGGATCATCGTGATACCTGTTTTCATCGGACTTTTGTTTGATCTTCTGGTGGTAGTCCCTTTGCGGGTTCCTGTGGATGAAAGTCCCGTGTTTCTTCTCTATCAGGATTGGGCTTTTGGGCTTATATTTCTCAAGATATGGACAAAATTGGTATTGCTGGGTCAAATGGCACCCCTTACAGATGACAGCTGGCGAATAAAGTTCGAGCAAGTCAAAGCAGATGGGTTTTCTCGGTTACGTGGATTATGGGTGCTTCGCGAAATTCTGTCACCAATACTCGTAAAGCTTTTGACAGCACTCTGTGTGCCATATGTCTTTGCTCGTGGTTTGTTCCCTCTTTTGGGATACTCTCTTATTGTAAATTCTGCAGTTTATCGATTTGCCTGGTCTGGATGCCTTGTGCTGGGATTGTTATGGTATTGGATTAGAAAGCTTCATCAGTGGTTTATGGATCTTCACAACTCTATCCGAGATGATAGATATCTGATAGGGAGGAGATTACACAACTTTAGAGAAGAGAAGAAGATAGAGGAGGGAAGCAGCGGTAATCCGTCACAAGCTGGGGGAGAGGTGTCAACAGATGAGCCTTTAATAGCTGCTGACGATGGCAATTTTGCTTCTGAAAATACTGAAGAAACTTTCTTGCCAATGATTGAGAGTTCCAGTTCCAGTTCTTTAGGGAGACATGGAGAAGCTGGAGCAAGTTTAAGGCAAAGACTCCCGAGTACCACTTCTGAGTAGTTTTCCTGGTGGGTCCTTCCAAGATATAATTCACTTTGAATGTTGCTAGATCGTTATCCTCCCAACGATCACCAAATTATTGGTTGTATCATGTAACTGTCATAATCAGCTTTCTGGGTGTTGTGCAAATTTTACAGCTCCAATGGAGTGCAATCTTGTGAATAACAGGAACATTTGAAAATAGATAATTATACATTGTTCCATCACCACAGTCGCAAAAGGCTTCTGTTAGCTTTGCTGTCATATTTATTCATGTTATGCTACAAAAAGCATTTTGCATTTGCTGA

Ace file of the UniGene sp_v1.1_unigene819 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	protein binding / zinc ion binding; K10661 E3 ubiquitin-protein ligase MARCH6 [EC:6.3.2.19]	-	-	61%
FL-Next	tr=Putative uncharacterized protein Sb10g025050; Sorghum bicolor (Sorghum) (Sorghum vulgare).	-	-	39%
Blast2go	protein	-	-	75%

Source	ECs	Term	Type	e value	Identity
AutoFact	Ubiquitin--protein ligase.	EC:6.3.2.19	-	0.0	61%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: tr_plants

Fln subject: C5Z6M1

Fln msg: Overlapping hits, possible frame ERROR between 988 and 960, No stop codon before M and low homology subject, your sequence is longer than subject: 851 - 743

Fln protein: MQAARLEAHVEQMFDGGDDADGIEDVPFDELVGMQGPVFHLIENAITVLASNMIFLGVVIFLPFQLGRILLSIASQLAVTTSSALLTAGMSSSSGSDLALSDITLKNSLESAANMSASALTKELLQGNMSTGVLHSDGGSNVTKSGGSVEVLTEALLASLRVSDAATLSIGYMVILLFVLCYLGLIALIRYSRGEPLTVGRIYGIASVAEAAPSLARSLLSGVKYFFTMVKVAFLLLVExxxxxxxxxxxWLDVCTISMLGTTLSQRVGFFWESPLTSSFLHWLVGIVYMLQISIFVSLLREVLRPGVLYFLRDPADPNYNPFRDLIDDPIHKHARRVLLSVVVYGSLIVLLIFLPVKLAMFLAPSLYPLDTSMSDPFTEIPADMLLFHICIPFAIDHFRPRATFKNVLFHWFSVTGWMLGLSDFLLPNPEDGNGQDGLNGHQRIQGRLQDVRRHGVQEQNRHRLALPAAGVAGRVVHENEGATFEENDGDEQDDSEEYKFAICIVLLLFGAWLTMLCFNSAMFVIPISLGRAMFASIPRLPITHGIKCNDLYAFNIGCYVIWAAAAGVGYLVDYLRVHSFRVLFMQFIKWSLIVLKSCILLSLWIIVIPVFIGLLFDLLVVVPLRVPVDESPVFLLYQDWAFGLIFLKIWTKLVLLGQMAPLTDDSWRIKFEQVKADGFSRLRGLWVLREILSPILVKLLTALCVPYVFARGLFPLLGYSLIVNSAVYRFAWSGCLVLGLLWYWIRKLHQWFMDLHNSIRDDRYLIGRRLHNFREEKKIEEGSSGNPSQAGGEVSTDEPLIAADDGNFASENTEETFLPMIESSSSSSLGRHGEAGASLRQRLPSTTSE*

Protein Length: 852

Fln nts: GTGTGCAACCTCTCTGCGCGAGTTCTTCAGACATCTTCGTGAAATTGAGAGGCCAAATCCTGAGAGGGAAGATGATGGCATCAACAGGCAAAATGGGGAAAGAGCAGGAAGGCGTCTACCTGTACCAGATGGAGCTCATCTTGTTGGGGGAAATGGTGAGGGTGGTGGAGCTGGCCAGGAAAACATTGGTGCAGGGCAATTGATCAGGAGAAATGCACAAAATGTAGCGGCTCGTGTGGAA_-_ATGCAAGCGGCACGACTTGAGGCACATGTTGAGCAAATGTTTGATGGTGGAGATGATGCGGATGGTATTGAGGATGTGCCATTTGATGAACTTGTGGGGATGCAAGGTCCTGTGTTTCATTTGATTGAAAATGCAATAACGGTGCTGGCTAGTAACATGATATTTCTTGGTGTTGTAATCTTTCTACCTTTCCAACTAGGTCGGATACTTCTTTCCATTGCATCTCAACTTGCTGTCACCACATCTAGTGCATTACTTACAGCAGGAATGTCTAGTTCTTCTGGGTCAGATTTGGCTCTCTCTGACATTACTTTAAAGAATAGTCTCGAGTCAGCTGCAAATATGTCAGCAAGTGCCCTGACCAAGGAGCTACTTCAAGGGAATATGAGCACTGGTGTGCTTCACTCTGATGGTGGTTCAAATGTTACTAAATCTGGGGGATCTGTGGAAGTATTGACAGAAGCCTTGCTTGCTTCGTTGCGGGTTTCTGATGCTGCAACTCTTTCTATCGGTTACATGGTTATTTTGCTTTTTGTTTTATGTTATCTTGGCTTAATAGCCTTGATACGCTACAGCAGAGGTGAGCCTTTGACAGTGGGTAGGATCTATGGTATAGCTTCGGTGGCAGAGGCTGCTCCTTCTCTTGCAAGGTCGTTGCTGTCTGGAGTAAAATACTTTTTCACCATGGTGAAGGTTGCTTTTCTTTTGCTAGTGGAGxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxTGGCTTGATGTGTGCACCATTAGCATGCTTGGAACAACATTGTCTCAGAGGGTGGGCTTTTTCTGGGAGTCACCTTTAACAAGTTCATTCCTTCACTGGCTTGTTGGGATTGTGTATATGTTACAAATAAGCATCTTTGTCAGCCTTCTTCGAGAGGTACTTCGCCCTGGTGTCCTATACTTTCTCAGAGATCCTGCTGATCCAAATTATAATCCATTCCGAGATCTCATTGATGATCCGATACATAAACATGCACGAAGGGTCTTGCTGTCTGTTGTGGTTTATGGAAGCTTGATTGTCTTGCTGATATTCCTACCAGTCAAACTTGCAATGTTTTTGGCACCTTCACTATACCCTTTGGATACATCGATGTCAGATCCATTTACAGAGATCCCAGCAGACATGCTTTTGTTTCATATCTGTATTCCATTTGCCATTGATCACTTCAGGCCACGGGCAACTTTTAAGAATGTTCTTTTCCACTGGTTTTCTGTTACTGGTTGGATGCTTGGATTGTCTGACTTCCTCTTGCCAAACCCCGAGGACGGGAATGGTCAAGATGGTTTGAATGGTCATCAAAGAATACAGGGAAGGTTACAAGATGTTCGTCGCCATGGTGTCCAAGAACAGAATCGCCATAGATTAGCACTTCCTGCTGCTGGGGTTGCTGGGAGGGTGGTGCATGAAAATGAGGGTGCGACGTTTGAAGAGAATGATGGAGATGAACAAGATGATTCAGAAGAATATAAATTTGCTATCTGCATTGTATTGCTGCTATTTGGGGCATGGTTAACCATGCTCTGCTTCAACTCAGCAATGTTTGTGATACCTATTTCGTTAGGGCGGGCAATGTTTGCTTCAATTCCACGGCTTCCAATTACCCATGGCATTAAGTGCAACGATCTTTATGCCTTCAATATCGGATGTTATGTCATTTGGGCTGCGGCAGCTGGAGTTGGATATTTAGTAGACTATTTGAGAGTTCACAGCTTCAGAGTTCTTTTCATGCAGTTCATAAAATGGTCTTTAATAGTTCTGAAAAGTTGTATTCTTCTATCATTATGGATCATCGTGATACCTGTTTTCATCGGACTTTTGTTTGATCTTCTGGTGGTAGTCCCTTTGCGGGTTCCTGTGGATGAAAGTCCCGTGTTTCTTCTCTATCAGGATTGGGCTTTTGGGCTTATATTTCTCAAGATATGGACAAAATTGGTATTGCTGGGTCAAATGGCACCCCTTACAGATGACAGCTGGCGAATAAAGTTCGAGCAAGTCAAAGCAGATGGGTTTTCTCGGTTACGTGGATTATGGGTGCTTCGCGAAATTCTGTCACCAATACTCGTAAAGCTTTTGACAGCACTCTGTGTGCCATATGTCTTTGCTCGTGGTTTGTTCCCTCTTTTGGGATACTCTCTTATTGTAAATTCTGCAGTTTATCGATTTGCCTGGTCTGGATGCCTTGTGCTGGGATTGTTATGGTATTGGATTAGAAAGCTTCATCAGTGGTTTATGGATCTTCACAACTCTATCCGAGATGATAGATATCTGATAGGGAGGAGATTACACAACTTTAGAGAAGAGAAGAAGATAGAGGAGGGAAGCAGCGGTAATCCGTCACAAGCTGGGGGAGAGGTGTCAACAGATGAGCCTTTAATAGCTGCTGACGATGGCAATTTTGCTTCTGAAAATACTGAAGAAACTTTCTTGCCAATGATTGAGAGTTCCAGTTCCAGTTCTTTAGGGAGACATGGAGAAGCTGGAGCAAGTTTAAGGCAAAGACTCCCGAGTACCACTTCTGAGTAG___TTTTCCTGGTGGGTCCTTCCAAGATATAATTCACTTTGAATGTTGCTAGATCGTTATCCTCCCAACGATCACCAAATTATTGGTTGTATCATGTAACTGTCATAATCAGCTTTCTGGGTGTTGTGCAAATTTTACAGCTCCAATGGAGTGCAATCTTGTGAATAACAGGAACATTTGAAAATAGATAATTATACATTGTTCCATCACCACAGTCGCAAAAGGCTTCTGTTAGCTTTGCTGTCATATTTATTCATGTTATGCTACAAAAAGCATTTTGCATTTGCTGA

Fln Alignment:

sp_v1.1_unigene819___TLKNSLESAANMSASALTKELLQGNMSTGVLHS----DGGSNVTKSGGSVEVLTEALLASLRVSDAATLSIGYMVILLFVLCYLGLIALIRYSRGEPLTVGRIYGIASVAEAAPSLARSLLSGVKYFFTMVKVAFLLLVExxxxxxxxxxxxDVCTISMLGTTLSQRVGFFWESPLTSSFLHWLVGIVYMLQISIFVSLLREVLRPGVLYFLRDPADPNYNPFRDLIDDPIHKHARRVLLSVVVYGSLIVLLIFLPVKLAMFLAPSLYPLDTSMSDPFTEIPADMLLFHICIPFAIDHFRPRATFKNVLFHWFSVTGWMLGLSDFLLPNPEDGNGQDGLNGHQRIQGRLQDVRRHGVQEQNRHRLALPAAGVAGRVVHENEGATFEENDGDEQDDSEEYKFAICIVLLLFGAWLTMLCFNSAMFVIPISLGRAMFASIPRLPITHGIKCNDLYAFNIGCYVIWAAAAGVGYLVDYLRVHSFRVLFMQFIKWSLIVLKSCILLSLWIIVIPVFIGXXXXXXXXXXXXXXXDESPVFLLYQDWAFGLIFLKIWTKLVLLGQMAPLTDDSWRIKFEQVKADGFSRLRGLWVLREILSPILVKLLTALCVPYVFARGLFPLLGYSLIVNSAVYRFAWSGCLVLGLLWYWIRKLHQWFMDLHNSIRDDRYLIGRRLHNFREEKKIEEGSSGNPSQAGGEVSTDE
C5Z6M1_____________TLKSALNVVKNFSSDSNNEGVI-GHVIEVVTQSLKINATGLSVIQGTGKSSLMKGTTIGSSYLSDLTTLAVGYMFIFCLVFLYIGSLALLRYARGERFTIGRLYGIATILEAIPSLCRQFFAGMKHLMTMVKVAFLLVIExxxxxxxxxxxxDVCTLKMLGTTIAQRVEFFTMSPLASSSIHWLVGIVYMLQISIFVSLLRGVLRNGVLYFLRDPADPNYNPFRDLIDDPVHKHARRVLLSVAVYGSLIVMLVFLPVKLAMRVAPSIFPLDITIFDPFTEIPVDVLLFQICIPFAIEHFKPRATIKSLLHHWFAAVGWALGLTDFLLPKPEENGGQENWNGRAERRDRGHGGREMVAPQVEQRMIQHVAAEDNGRGNANEANDATEESDVDDQGDS-EYGFVLRIVLLLVLAWVTLLIFNAGMIVIPISLGRLVFEAVPRLPITHGIKCNDLFSFSIGCYILWSAAAGTRYAIDYIRSRQLGFLVQQICKWCSIVLKSSFLLSIWIFVIPVLIGLLFELLVIVPMRVPIDESPVFLLYQDWALGLIFLKIWTRLVMLDQMAPLVDESWRSKFERVRDDGFSRLRGLWVLHEIIMPIVTKLLTALCVPYVLARGIFPVLGYPLIVNSAVYRFAWLGCLIFSALFFCGKRFHVWFTNLHNSIRDDRYLIGRRLHNFG-----EDSHSPEPSESGATIGSDD

SNPs (tot: 29)

Position	A %	C %	G %	T %	Probability
1195		10		90	0.999898
1197	10		90		0.999898
1352		88	11		0.923564
1361		88		11	0.923564
1364	11	55		33	1.0
1365		11		88	0.923564
1505		83	16		0.994512
1703			12	87	0.992554
1720		14	85		0.993533
1959	12		87		0.992554
2110			60	40	1.0
2114	60			40	1.0
2115	60		40		1.0
2117	60	40			1.0
2119		60	40		1.0
2120		80		20	0.995493
2124			40	60	1.0
2125		60	40		1.0
2131			40	60	1.0
2199	87	12			0.992554
2200		12	87		0.992554
2202			87	12	0.992554
2249		20		80	0.995493
2250		20	80		0.995493
2254	80			20	0.995493
2868		16		83	0.994512
2871	66		16	16	0.988813
2872		16		83	0.994512
3035		20		80	0.995493

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain