SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene773

Length: 1153 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

This UniGene was originaly assembled in antisense
ACE File: antisense
Fasta: sense

UniGene Fasta (sense)

Ace file of the UniGene sp_v1.1_unigene773 (antisense)

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	E2Fa2-2; transcription factor E2F	-	-	68%
FL-Next	Isoform 2 of Transcription factor E2FB OS=Arabidopsis thaliana GN=E2FB	-	-	68%
Blast2go	transcription factor e2f	-	-	81%

Source	InterPros	Term	Type	Identity
Blast2go	Transcription factor E2F/dimerisation partner (TDP)	IPR003316	-	81%
Blast2go	Winged helix-turn-helix transcription repressor DNA-binding	IPR011991	-	81%
Blast2go	E2F Family	IPR015633	-	81%

Full-Lengther Next Prediction

Fln status: Putative N-terminus

Fln database: sp_plants

Fln subject: Q9FV71-2

Fln msg: Distance to subject end: 144 aas, Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 272 - 466

Fln protein: MKWKTETEEHEMESNELPSGQGYVEADTGAGNMTVSGTGGRRYNRSKIAKRAKIGPQTPGSNIGSPLGNAPTPISSCRYDSSLGLLTKKFISLVKQADDGILDLNKAADTLEVQKRRIYDITNVLEGIGLIEKRLKNRICWKGLNVSRPGEVEDESTVLQAEVDSLNLEECKLDDCIRDMQERLRILSEDDRNRRWLYVTDEDIKKLPCFQNDTLIAIKAPHGTTLEVPDPDEAVEYPQRRYQILLRSTMGPIDVYLVSQFEENIEEMNPVD

Protein Length: 273

Fln nts: CGTCGTGACCTTGGCTGTCACTCATCCCTCGCCCGCCTACTCCACATATATCCGAACTCAATTCAGACATTCAAACCCCATAACCTCCCACTTCTCACGAGCGGTCTTTCAATACCGTTGAAGGCAAGGCGGGTGTAATTATGGTAATGCCAGAGCTGGATTGCAGTGACACCCAAATGCTTTTCAGAAGGCCTCCTCTGATGGGTATCTGACATTTCTTCAAAACCTGGTTTAAGATTGGGTATGTGAAAGTGACAATGATCAAACATGGATAAAGCTGGTTTTATGGGTTCAGCCAGACGAGTCGATGTACTATCTGCATTCGGGCTGAAGACAG_-_ATGAAGTGGAAGACTGAAACGGAGGAACATGAGATGGAGTCCAATGAATTGCCATCAGGGCAAGGATATGTAGAAGCAGATACAGGTGCTGGAAATATGACTGTTTCAGGCACAGGTGGCAGGCGTTATAATCGGTCTAAGATTGCAAAGCGTGCAAAGATTGGTCCTCAGACACCTGGATCAAACATTGGTTCTCCTTTGGGCAATGCTCCTACTCCAATCAGCAGCTGTCGTTATGATAGCTCTCTTGGGTTGTTAACAAAGAAGTTTATTAGTCTAGTCAAGCAGGCTGATGATGGTATTCTTGATCTTAACAAGGCTGCAGATACTTTGGAAGTCCAAAAGCGCAGAATATATGACATAACCAATGTGCTTGAGGGGATAGGACTAATTGAGAAACGCCTGAAAAATAGAATTTGCTGGAAGGGTCTTAATGTGTCGAGACCTGGAGAGGTTGAAGATGAATCAACAGTTCTCCAGGCTGAAGTTGACAGTCTCAATCTTGAAGAATGCAAATTAGATGATTGCATAAGAGATATGCAGGAGCGATTAAGGATCTTGAGTGAAGATGACAGGAATAGAAGATGGCTTTATGTTACTGATGAAGACATTAAGAAGCTACCATGTTTTCAGAATGATACGCTTATAGCAATAAAGGCTCCTCATGGAACCACACTTGAAGTTCCAGATCCAGATGAGGCAGTAGAATACCCACAAAGGCGGTACCAGATTCTTTTACGAAGTACCATGGGGCCAATTGATGTTTATCTTGTAAGCCAATTTGAGGAGAATATTGAAGAAATGAATCCAGTTGAT

Fln Alignment:

sp_v1.1_unigene773___EADTGAGNMTVSGTGGRRYNRSKIAKRAKIGPQTPGSNIGSPLGNAPTPISSCRYDSSLGLLTKKFISLVKQADDGILDLNKAADTLEVQKRRIYDITNVLEGIGLIEKRLKNRICWKGLNVSRPGEVEDESTVLQAEVDSLNLEECKLDDCIRDMQERLRILSEDDRNRRWLYVTDEDIKKLPCFQNDTLIAIKAPHGTTLEVPDPDEAVEYPQRRYQILLRSTMGPIDVYLVSQFEENIEEMNPVD
Q9FV71-2___________ELNTGVLQTPVSGKGGKAKKTSRSAKSNKSGTLASGSNAGSP-GNNFAQAGTCRYDSSLGLLTKKFINLIKQAEDGILDLNKAADTLEVQKRRIYDITNVLEGIGLIEKTLKNRIQWKGLDVSKPGETIESIANLQDEVQNLAAEEARLDDQIRESQERLTSLSEDENNKRLLFVTENDIKNLPCFQNKTLIAVKAPHGTTLEVPDPDEAGGY-QRRYRIILRSTMGPIDVYLVSQFEESFEDIPQAD

SNPs (tot: 37)

Position	A %	C %	G %	T %	Probability
573	80			20	0.995493
575	20	80			0.995493
576		20		80	0.995493
578			20	80	0.995493
581		20		80	0.995493
584		80	20		0.995493
586	80			20	0.995493
590	20	80			0.995493
591		20		80	0.995493
701		81		18	0.999994
702		81	18		0.999994
703	20			80	0.999996
707	20	80			0.999996
709	20			80	0.999996
711	60	20			0.999999
712	80	20			0.999996
714	22	77			0.999999
715	77		22		0.999999
721			22	77	0.999999
723		20		80	0.999996
724			20	80	0.999996
797		66		33	1.0
971	14		85		0.993533
972	85		14		0.993533
1048	66		33		1.0
1077			75	25	0.996473
1079	25		75		0.996473
1086	75	25			0.996473
1087	25			75	0.996473
1091		25		75	0.996473
1092	25		75		0.996473
1094	75		25		0.996473
1095			25	75	0.996473
1097		25	75		0.996473
1099		25	75		0.996473
1100			25	75	0.996473
1101	25			75	0.996473

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain