SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene488

Length: 2069 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene488
CGTCGTGACCTTGGCTGTCACTCAAAGCAATTGAATCGAAGGGCAAAATCAGTTACAGTTGGGCGACTTCAAATAGTGTCTACATTGCACAACGTTGGACTTTGAAGGAGCATGATATCATCTGACGGCGATGCATTGACTGCATCATCGGACAAACCTGGATTCATCTCAATCCCAATACACAAACGCCAACGTTGTTCTCTGAGAAGGCTCACGAAATCTCTAGACTTTCTTAGGGTATTCAGGAGCCAATTGTTTGAAGAGACAAAACAATTCTGTCATCGATGGTTCGTTCAAATGGTTTCATATATGCATGGTGGATCGCACTTCGATTGGGCAAAGGTTCAGTTAAGTCAGTTGGACGCCAGGAGAAATTGCGGCAAGCGAGTTTGTACGTTCCAGCTTCTGGCCTCTTTAATAATCCTGACCTTGGCTGACAATCAATTGGGACAAATGCCACTCAGTTCGTCATGGCAATATATTTTGCAGTAAAGGGCCTGGAAATGATTTTATAAGCAGGGGCTTTCATTCAACAGCTACATATTATATGGCTGCTAAGGACTACTATGAAATTCTTGGAGTAAATAAGAGTGCTAGTGCATCAGAAATCAAGAAGGCTTACTATGCGCTTGCAAAACAACATCATCCAGATGTTAATAAGAGTGATCCAGAAGCAGAGAAAAAATTTCAGGATATTCAACGTGCTTATGAGGTATTGAAAGATGATGAAAAACGATCATTATATGACCAGATGGGTCCTGAGGCCTTTGAACAGGCATCAGCAGGTGGTGCACCAGGTGGTCAGGGAGGTCCAGGATTTGGTGGGTTTGGCGGATTTGGTGGATTTGACTTTGGTGATATTTTTGGAGGTGGTCTAAATGAGGCCTTGAAGAACATGTTTCATCAAAAGTCCTTTGGAGGCCAGGATATCAAGATAACAATGGAATTGTCTTTCATGGAAGCAGTTCAAGGATGCACTAAGAACCTTTCTTATGACGCTGATGTGCGTTGTTCAGCATGCGATGGGACTGGAATGCCTGCTGGAACTAAACCTCAGACATGCAAAGTTTGTAGAGGTTCAGGAACGATATCTATGCAGCGTGGCCCTTTTAGAATGCAAAGTACGTGTTCAGAGTGCGGTGGAATTGGAAAGGTTATAAAGGAGTTTTGTAAGTCATGTCGTGGCAAAAGGGTTGTTAGAGGATCGAAAAGAGTAACTTTGGATGTAATACCAGGTGTTGATAATGGAGAAACATTGAGGATGCATGGATATGGTGGGGAAGACCCAGAAGGTGCCCAACCAGGAGACCTCTATGTTGCTCTTAAGGTCCGTGATGATCCAATATTCCGTAGAGAAGGAGCTGACATTCATGTGGAGGCAGTCATAAGCTTCACTCAGGCAATACTTGGTGGGACTATCCAGGTGCCAACTCTAACAGGAGACATTGTGTTGAAGGTTCGTGAAGGGACACAGCATGGTCAGAAGGTTGTGTTGAAAGGAAAGGGCATTAGATTACGGAATTCTACCCAGTATGGCAATCAGTATGTACATTTTCGAGTCATTATTCCAACGAATTTGACACAAAGACAACGGATGCTTATAGAGGAGTTTGCAAAAGAGGAGAACAGTGAAGATGACAAAAGTGCTGCCGGGGCATCTGGCTGAACCCTGATGTTTCCTTTTTAATGAGGCGATTCTGTCACCCATTTGAAAGGCAAGCTCTGTCTTTGGGATTGTGAGGTGGTTGTCATAATGAAATACACTCTCTTGTCCTCATTTCATGTATAGCTGTCAAGGTTAACTTTTATTTGAATGGAACTTACGTGATTTGATTTTGTATTAATAGCATTCTTCTTGAATCTCTATTTCTATAAAATGACGATGAATTAAAATGCATGATATAGTGTTATCTGGTCAAACAATTGGCCAATAACTCACTCATTCTTGTAGTTACGATGGACTTTCCGTACATGATTAGCACTTGGTTCATTATTTTGTTTCAATGGGGTCATACTGAAGAATGCACTGTATTACAATTTTATGGCTGAAATGTAGCAATATATATTTT

Ace file of the UniGene sp_v1.1_unigene488

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	pco077147; LOC100282679; K03686 molecular chaperone DnaJ	-	-	56%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	67%
Blast2go	chaperone protein	-	-	74%

Source	GOs	Term	Type	Identity
Blast2go	embryo sac cellularization	GO:0009558	Biological Process	74%
Blast2go	chaperone mediated protein folding requiring cofactor	GO:0051085	Biological Process	74%
Blast2go	polar nucleus fusion	GO:0010197	Biological Process	74%
Blast2go	nuclear membrane fusion	GO:0000740	Biological Process	74%
Blast2go	synergid death	GO:0010198	Biological Process	74%
Blast2go	mitochondrion	GO:0005739	Cellular Component	74%

Source	InterPros	Term	Type	Identity
Blast2go	Heat shock protein DnaJ, cysteine-rich domain	IPR001305	-	74%
Blast2go	Heat shock protein DnaJ, N-terminal	IPR001623	-	74%
Blast2go	Chaperone DnaJ, C-terminal	IPR002939	-	74%
Blast2go	Heat shock protein DnaJ	IPR003095	-	74%
Blast2go	HSP40/DnaJ peptide-binding	IPR008971	-	74%
Blast2go	Chaperone DnaJ	IPR012724	-	74%
Blast2go	Heat shock protein DnaJ, conserved site	IPR018253	-	74%

Full-Lengther Next Prediction

Fln status: Putative Complete

Fln database: coniferopsida.fasta

Fln subject: A9NVQ6

Fln msg: Unexpected STOP codon in 5 prime region, your sequence is shorter than subject: 413 - 463

Fln protein: PWLTINWDKCHSVRHGNIFCSKGPGNDFISRGFHSTATYYMAAKDYYEILGVNKSASASEIKKAYYALAKQHHPDVNKSDPEAEKKFQDIQRAYEVLKDDEKRSLYDQMGPEAFEQASAGGAPGGQGGPGFGGFGGFGGFDFGDIFGGGLNEALKNMFHQKSFGGQDIKITMELSFMEAVQGCTKNLSYDADVRCSACDGTGMPAGTKPQTCKVCRGSGTISMQRGPFRMQSTCSECGGIGKVIKEFCKSCRGKRVVRGSKRVTLDVIPGVDNGETLRMHGYGGEDPEGAQPGDLYVALKVRDDPIFRREGADIHVEAVISFTQAILGGTIQVPTLTGDIVLKVREGTQHGQKVVLKGKGIRLRNSTQYGNQYVHFRVIIPTNLTQRQRMLIEEFAKEENSEDDKSAAGASG*

Protein Length: 414

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAAGCAATTGAATCGAAGGGCAAAATCAGTTACAGTTGGGCGACTTCAAATAGTGTCTACATTGCACAACGTTGGACTTTGAAGGAGCATGATATCATCTGACGGCGATGCATTGACTGCATCATCGGACAAACCTGGATTCATCTCAATCCCAATACACAAACGCCAACGTTGTTCTCTGAGAAGGCTCACGAAATCTCTAGACTTTCTTAGGGTATTCAGGAGCCAATTGTTTGAAGAGACAAAACAATTCTGTCATCGATGGTTCGTTCAAATGGTTTCATATATGCATGGTGGATCGCACTTCGATTGGGCAAAGGTTCAGTTAAGTCAGTTGGACGCCAGGAGAAATTGCGGCAAGCGAGTTTGTACGTTCCAGCTTCTGGCCTCTTTAATAATCCTGACCTTGGCTGACAATCAATTGGGACAAATGCCACTCAGTTCGTCATGGCAATATATTTTGCAGTAAAGGGCCTGGAAATGATTTTATAAGCAGGGGCTTTCATTCAACAGCTACATATTATATGGCTGCTAAGGACTACTATGAAATTCTTGGAGTAAATAAGAGTGCTAGTGCATCAGAAATCAAGAAGGCTTACTATGCGCTTGCAAAACAACATCATCCAGATGTTAATAAGAGTGATCCAGAAGCAGAGAAAAAATTTCAGGATATTCAACGTGCTTATGAGGTATTGAAAGATGATGAAAAACGATCATTATATGACCAGATGGGTCCTGAGGCCTTTGAACAGGCATCAGCAGGTGGTGCACCAGGTGGTCAGGGAGGTCCAGGATTTGGTGGGTTTGGCGGATTTGGTGGATTTGACTTTGGTGATATTTTTGGAGGTGGTCTAAATGAGGCCTTGAAGAACATGTTTCATCAAAAGTCCTTTGGAGGCCAGGATATCAAGATAACAATGGAATTGTCTTTCATGGAAGCAGTTCAAGGATGCACTAAGAACCTTTCTTATGACGCTGATGTGCGTTGTTCAGCATGCGATGGGACTGGAATGCCTGCTGGAACTAAACCTCAGACATGCAAAGTTTGTAGAGGTTCAGGAACGATATCTATGCAGCGTGGCCCTTTTAGAATGCAAAGTACGTGTTCAGAGTGCGGTGGAATTGGAAAGGTTATAAAGGAGTTTTGTAAGTCATGTCGTGGCAAAAGGGTTGTTAGAGGATCGAAAAGAGTAACTTTGGATGTAATACCAGGTGTTGATAATGGAGAAACATTGAGGATGCATGGATATGGTGGGGAAGACCCAGAAGGTGCCCAACCAGGAGACCTCTATGTTGCTCTTAAGGTCCGTGATGATCCAATATTCCGTAGAGAAGGAGCTGACATTCATGTGGAGGCAGTCATAAGCTTCACTCAGGCAATACTTGGTGGGACTATCCAGGTGCCAACTCTAACAGGAGACATTGTGTTGAAGGTTCGTGAAGGGACACAGCATGGTCAGAAGGTTGTGTTGAAAGGAAAGGGCATTAGATTACGGAATTCTACCCAGTATGGCAATCAGTATGTACATTTTCGAGTCATTATTCCAACGAATTTGACACAAAGACAACGGATGCTTATAGAGGAGTTTGCAAAAGAGGAGAACAGTGAAGATGACAAAAGTGCTGCCGGGGCATCTGGCTGA___ACCCTGATGTTTCCTTTTTAATGAGGCGATTCTGTCACCCATTTGAAAGGCAAGCTCTGTCTTTGGGATTGTGAGGTGGTTGTCATAATGAAATACACTCTCTTGTCCTCATTTCATGTATAGCTGTCAAGGTTAACTTTTATTTGAATGGAACTTACGTGATTTGATTTTGTATTAATAGCATTCTTCTTGAATCTCTATTTCTATAAAATGACGATGAATTAAAATGCATGATATAGTGTTATCTGGTCAAACAATTGGCCAATAACTCACTCATTCTTGTAGTTACGATGGACTTTCCGTACATGATTAGCACTTGGTTCATTATTTTGTTTCAATGGGGTCATACTGAAGAATGCACTGTATTACAATTTTATGGCTGAAATGTAGCAATATATATTTT

Fln Alignment:

sp_v1.1_unigene488___CSKGPGNDFISRGFHSTATYYMAAKDYYEILGVNKSASASEIKKAYYALAKQHHPDVNKSDPEAEKKFQDIQRAYEVLKDDEKRSLYDQMGPEAFEQASXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLNEALKNMFHQKSFGGQDIKITMELSFMEAVQGCTKNLSYDADVRCSACDGTGMPAGTKPQTCKVCRGSGTISMQRGPFRMQSTCSECGGIGKVIKEFCKSCXXXXXXXXXXXXTLDVIPGVDNGETLRMHGYGGEDPEGAQPGDLYVALKVRDDPIFRREGADIHVEAVISFTQAILGGTIQVPTLTGDIVLKVREGTQHGQKVVLKGKGIRLRNSTQYGNQYVHFRVIIPTNLTQRQRMLIEEFAKEENSEDDKSAAGASG
A9NVQ6_____________CNKIFRFESAKRRFHATGTLGMAARDLYDILGISKDAGQGEIKKAYYALAKKHHPDVNKGDPDAEKKFQEIQRAYEVLKDDEKRSLYDRVGPEGFAQAEAGGGAGGPGSGDFGFGYGGFGFEDMFGGG--MNEALRNMFNQGSFGGGDVKMTLELSFMEAVQGCTKNLSFQTSVTCTSCNGSGVPAGTKPQTCRVCKGSGTIKMQRGAFRLESTCSTCGGSGTLVKEFCKTCGGDRVVKGQKKCTLDIMAGIDNDETLRVLGRGGADPEGGHPGDLLVTIKVREDPVFRRDGADIHVDENISISQAILGGTIQVPTLTGDVVLKVRQGTQHGQKVALKGKGIKSRHSRHYGNQYVHFRVSIPTNITQRQRSLIEEFAKEENRDEEKRAAEASG

SNPs (tot: 29)

Position	A %	C %	G %	T %	Probability
518	85		14		0.993533
519	85		14		0.993533
708	16		83		0.994512
918			16	83	0.994512
920		16		83	0.994512
922			83	16	0.994512
925	16		83		0.994512
928	83			16	0.994512
931	83	16			0.994512
933	83		16		0.994512
937	83	16			0.994512
941	83	16			0.994512
998		16		83	0.994512
1000	83			16	0.994512
1007		16		83	0.994512
1008		16	83		0.994512
1010			83	16	0.994512
1011		83	16		0.994512
1013		16		83	0.994512
1024		20	80		0.995493
1025	20	80			0.995493
1143		80		20	0.995493
1370	80		20		0.995493
1446	87	12			0.992554
1477	87		12		0.992554
1768	91			8	0.999766
1926	88			11	0.923564
1927	88		11		0.923564
1928		88	11		0.923564

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain