SustainPineDB

Assembly Name: SustainPine v1.1

UniGene Name: sp_v1.1_unigene320

Length: 2878 nt

Origin of reads: Biogeco_1 Biogeco_2 Uagpf Uma

UniGene Fasta

>sp_v1.1_unigene320
CGTCGTGACCTTGGCTGTCACTCAAATTGGGGTCATGCAACGGCCAGTGACTCTCACTCTTCAGCAAACGTGCACCTGCAGGCCAGCGAGAAAATGGGGAAGGATAGGGCCCTTGTAGTTCTGGTAACCTTCTGTTGTTAGCAGCCTTGTCTGATACACTCAGAGACTTTCGCATTCGGACTTGCAAAGTTGGTTTACTCAAATAATATTCTTCGTCAAGTTCTTTGAGGGTTTTTAGGGAGGACGCTGGACTCGATCCGGCGACCCTTGCTGGTTTCTTCAGATAAGGCGCAATAATTCATTTATTGGGGTGCAATCGACGCTCTATAGAAGAAATCCCGGGATTCAGTCACTTATAATTACGGGCTATTGTTAGGTATTGCGCGGTTCCTGCGGAGAAAATGGACAGCAGCAGCAGCAGCATTGAAATTACGCGGCGCCGCTTGGAAACGATCGGCGGACATTTAAATTCTGTCGAATCCGAATTAATTGGACAGCTTAAGATAAATTGCACTTCGGAAGAAATTTCTGCGGATCAAGGATACAGTGTCATTCTTCCTGAAAAATTGCGGACAGGAAAATGGAATGTTTATAGATCAGTGCGTGCACCTTTGAAGCTTGTTGAGAGATTTCCAGATCATCCTGAGATTGGAACATTGCATGACAATGTTGTACATGCAGTGGAATCCTATCAAGATCTCAAGTATCTCGGAACAAGAGTTCGTGCGGATGGAACTATTGGAGAATATAAGTGGATGACATATAGAGATGTAGGGGCAGCACGGAGTGCAATTGGTTCTGGGCTTGTCTATCATGGTATTCCAAAGGGAGCTTGCATTGGGCTCTACTTGATCAATAAACCAGAGTGGATTATCACTGAACATGCATGCTGTTCATACTCGTACATCTCAGTCCCTCTATATGACACACTTGGTCCAGATGCTGTGAAATACATCATAAACCATGCTGATATACAAGCTGTATTTTGTGCTCCCGAGAACTTGAATATTTTGCTGGGCTTCTTATCAGATCTTTCCTCTGTTCGTCTTATTGTGGTTGTTGGGGGGACAGAACAAGTTAAGCCTTCTCTTTCTTCTACAACTAGAGTTGAGGTCATCTCTTATTCAAAGTTGGAAGATCAGGGTAGTTGCAATCTTCAACCATTTCGACCCCCATTGCCAGAAGATGTTGCCACAATTTGCTACACAAGTGGAACAACTGGAACTCCAAAGGGAGCTGTATTAACCCATGGCAATCTTATTGCTAGTATCGCTGGTTCCCTTCTTTCCATCAAGCTTTTCTCTACAGATGTCTATATCTCTTATCTTCCGTTGGCACACATTTATGAAAGGTTAATCACCATTGGTGTAACACACAGTGGAGCTGCCATGGGATTTTACCAAGGGGACAATATGAAGCTAATGGATGATATTGCAGTCTTGAGACCGACGTTATTTGCCAGTGTACCCAGACTTTACAATAAAATCTATTCAGGAATTTTGAGTGCTGTGAAGATGTCTGGTTCTCTCCGAGAAAGTCTATTCAATATTGCATTCAATGCTAAGAAGCAGGCAATTACCAATGGGAAAGTCCCATCATCTATATGGGACATGCTGGTTTTTAATAAAATAAAGGCAAAACTTGGGGGCCGTGTTCGCATGATGCTTTCTGGGGCATCACCCATATCTCCTGAAGTTATCGACTTTTTGAGAGTTTGCTTTGGTGGTAGGGTTTCTGAGGGTTATGGGATGACAGAGACAGCATGCATGATCTGCCTTTGTGAGGAGGATGACATTTCATCTGGTCATGTTGGACCACCTAGTCCTTCTTGCGAGGTAAAACTGGAAGATGTTCCTGAAATGAATTACACTTGTGATGACAAACCATATCCTCGGGGAGAAATTTGTGTGAGGGGACCCATTGTTTTTAAGGGATACTTCAAAGATGATGTGCAAACTCAGGAGATATTTGACGAGGAGGGCTGGTTGCACACTGGAGATATAGGGCTTTGGCTTCCAGGAGGACGATTGAGGATTATTGATCGGAAGAAAAATATTTTTAAGCTGGCACAAGGGGAGTATATTGCTCCTGAAAAAATTGAAAATGTTTATGCAACATGCAAATTTGTTGGACAATGCTTCATCTATGGTGATAGCTTGAACTCAAGTTTAGTGGCTGTTGTTGCCGTAGAGCCTGATGCTTTAAAAGCATGGGCCATGTCTGAAGGCATCCAGGTTGAAGATTTGAGTCAACTATGCTCTGACCCAAGAGCAAAAGAAGCCGTGTTAGCGGACATGGATGCTGTTGGACGTGAGGCCCAGCTAAGAGGCTTTGAATTTGCAAAAGCAGTGACATTGGTTGCAGAGCCTTTCAGCTTGGAGAATGGACTACTTACTCCAACATTTAAGATTAAAAGGCCACAAGCGAAGGCTTATTTTGCAAAAGCCATATCGGACATGTATTCTGATCTTGCTATTCAGGAAAGATCTCTTCCTAGAAGTAGTTTGTGAATGCACAGAAGAAATTGTAATTGTACAAGAACCTAACCGTCACACATGTATGATCTGATTCATTTGTTAACTTAAGCATTAACTTGCATGGTTTCATACTAATTTCTTGCCCTTTTTGACTTTATGTTTGTATTATGTTGATTTCTTTAGCCCAAATGTAACTAAACTATTTGAGGAATCTTACCAAATCACCATGGACATGCTTCTAGTCATATAATGAGGGCTCCTTTCAATGAACTTTGATGCAAGGATAAAATATCCACCAAGGAAATTCATTAAATGTTTGTAAACTCTATGAATTATTGTCGGAGCCAAGCAATGATTCTATATACGCTAATTTAAGGAAATGATAAATCGAGTTTTGTATT

Ace file of the UniGene sp_v1.1_unigene320

Annotations

Source	Descriptions	Term	Type	Identity
AutoFact	Acyl-CoA synthetase (EC:6.2.1.3); K01897 long-chain acyl-CoA synthetase [EC:6.2.1.3]	-	-	69%
FL-Next	tr=Putative uncharacterized protein; Picea sitchensis (Sitka spruce) (Pinus sitchensis).	-	-	40%
Blast2go	amp-binding protein	-	-	82%

Source	ECs	Term	Type	e value	Identity
AutoFact	Long-chain-fatty-acid--CoA ligase.	EC:6.2.1.3	-	0.0	69%

Source	KEGGs	Term	Type	e value	Identity
AutoFact	Fatty acid metabolism	00071		0.0	69%
AutoFact	Metabolic pathways	01100		0.0	69%

Source	InterPros	Term	Type	e value	Identity
Blast2go	AMP-dependent synthetase/ligase	IPR000873	-	0.0	82%
Blast2go	AMP-binding, conserved site	IPR020845	-	0.0	82%

Full-Lengther Next Prediction

Fln status: Complete

Fln database: coniferopsida.fasta

Fln subject: D5ACR7

Fln msg: your sequence is longer than subject: 703 - 197

Fln protein: MDSSSSSIEITRRRLETIGGHLNSVESELIGQLKINCTSEEISADQGYSVILPEKLRTGKWNVYRSVRAPLKLVERFPDHPEIGTLHDNVVHAVESYQDLKYLGTRVRADGTIGEYKWMTYRDVGAARSAIGSGLVYHGIPKGACIGLYLINKPEWIITEHACCSYSYISVPLYDTLGPDAVKYIINHADIQAVFCAPENLNILLGFLSDLSSVRLIVVVGGTEQVKPSLSSTTRVEVISYSKLEDQGSCNLQPFRPPLPEDVATICYTSGTTGTPKGAVLTHGNLIASIAGSLLSIKLFSTDVYISYLPLAHIYERLITIGVTHSGAAMGFYQGDNMKLMDDIAVLRPTLFASVPRLYNKIYSGILSAVKMSGSLRESLFNIAFNAKKQAITNGKVPSSIWDMLVFNKIKAKLGGRVRMMLSGASPISPEVIDFLRVCFGGRVSEGYGMTETACMICLCEEDDISSGHVGPPSPSCEVKLEDVPEMNYTCDDKPYPRGEICVRGPIVFKGYFKDDVQTQEIFDEEGWLHTGDIGLWLPGGRLRIIDRKKNIFKLAQGEYIAPEKIENVYATCKFVGQCFIYGDSLNSSLVAVVAVEPDALKAWAMSEGIQVEDLSQLCSDPRAKEAVLADMDAVGREAQLRGFEFAKAVTLVAEPFSLENGLLTPTFKIKRPQAKAYFAKAISDMYSDLAIQERSLPRSSL*

Protein Length: 704

Fln nts: CGTCGTGACCTTGGCTGTCACTCAAATTGGGGTCATGCAACGGCCAGTGACTCTCACTCTTCAGCAAACGTGCACCTGCAGGCCAGCGAGAAAATGGGGAAGGATAGGGCCCTTGTAGTTCTGGTAACCTTCTGTTGTTAGCAGCCTTGTCTGATACACTCAGAGACTTTCGCATTCGGACTTGCAAAGTTGGTTTACTCAAATAATATTCTTCGTCAAGTTCTTTGAGGGTTTTTAGGGAGGACGCTGGACTCGATCCGGCGACCCTTGCTGGTTTCTTCAGATAAGGCGCAATAATTCATTTATTGGGGTGCAATCGACGCTCTATAGAAGAAATCCCGGGATTCAGTCACTTATAATTACGGGCTATTGTTAGGTATTGCGCGGTTCCTGCGGAGAAA_-_ATGGACAGCAGCAGCAGCAGCATTGAAATTACGCGGCGCCGCTTGGAAACGATCGGCGGACATTTAAATTCTGTCGAATCCGAATTAATTGGACAGCTTAAGATAAATTGCACTTCGGAAGAAATTTCTGCGGATCAAGGATACAGTGTCATTCTTCCTGAAAAATTGCGGACAGGAAAATGGAATGTTTATAGATCAGTGCGTGCACCTTTGAAGCTTGTTGAGAGATTTCCAGATCATCCTGAGATTGGAACATTGCATGACAATGTTGTACATGCAGTGGAATCCTATCAAGATCTCAAGTATCTCGGAACAAGAGTTCGTGCGGATGGAACTATTGGAGAATATAAGTGGATGACATATAGAGATGTAGGGGCAGCACGGAGTGCAATTGGTTCTGGGCTTGTCTATCATGGTATTCCAAAGGGAGCTTGCATTGGGCTCTACTTGATCAATAAACCAGAGTGGATTATCACTGAACATGCATGCTGTTCATACTCGTACATCTCAGTCCCTCTATATGACACACTTGGTCCAGATGCTGTGAAATACATCATAAACCATGCTGATATACAAGCTGTATTTTGTGCTCCCGAGAACTTGAATATTTTGCTGGGCTTCTTATCAGATCTTTCCTCTGTTCGTCTTATTGTGGTTGTTGGGGGGACAGAACAAGTTAAGCCTTCTCTTTCTTCTACAACTAGAGTTGAGGTCATCTCTTATTCAAAGTTGGAAGATCAGGGTAGTTGCAATCTTCAACCATTTCGACCCCCATTGCCAGAAGATGTTGCCACAATTTGCTACACAAGTGGAACAACTGGAACTCCAAAGGGAGCTGTATTAACCCATGGCAATCTTATTGCTAGTATCGCTGGTTCCCTTCTTTCCATCAAGCTTTTCTCTACAGATGTCTATATCTCTTATCTTCCGTTGGCACACATTTATGAAAGGTTAATCACCATTGGTGTAACACACAGTGGAGCTGCCATGGGATTTTACCAAGGGGACAATATGAAGCTAATGGATGATATTGCAGTCTTGAGACCGACGTTATTTGCCAGTGTACCCAGACTTTACAATAAAATCTATTCAGGAATTTTGAGTGCTGTGAAGATGTCTGGTTCTCTCCGAGAAAGTCTATTCAATATTGCATTCAATGCTAAGAAGCAGGCAATTACCAATGGGAAAGTCCCATCATCTATATGGGACATGCTGGTTTTTAATAAAATAAAGGCAAAACTTGGGGGCCGTGTTCGCATGATGCTTTCTGGGGCATCACCCATATCTCCTGAAGTTATCGACTTTTTGAGAGTTTGCTTTGGTGGTAGGGTTTCTGAGGGTTATGGGATGACAGAGACAGCATGCATGATCTGCCTTTGTGAGGAGGATGACATTTCATCTGGTCATGTTGGACCACCTAGTCCTTCTTGCGAGGTAAAACTGGAAGATGTTCCTGAAATGAATTACACTTGTGATGACAAACCATATCCTCGGGGAGAAATTTGTGTGAGGGGACCCATTGTTTTTAAGGGATACTTCAAAGATGATGTGCAAACTCAGGAGATATTTGACGAGGAGGGCTGGTTGCACACTGGAGATATAGGGCTTTGGCTTCCAGGAGGACGATTGAGGATTATTGATCGGAAGAAAAATATTTTTAAGCTGGCACAAGGGGAGTATATTGCTCCTGAAAAAATTGAAAATGTTTATGCAACATGCAAATTTGTTGGACAATGCTTCATCTATGGTGATAGCTTGAACTCAAGTTTAGTGGCTGTTGTTGCCGTAGAGCCTGATGCTTTAAAAGCATGGGCCATGTCTGAAGGCATCCAGGTTGAAGATTTGAGTCAACTATGCTCTGACCCAAGAGCAAAAGAAGCCGTGTTAGCGGACATGGATGCTGTTGGACGTGAGGCCCAGCTAAGAGGCTTTGAATTTGCAAAAGCAGTGACATTGGTTGCAGAGCCTTTCAGCTTGGAGAATGGACTACTTACTCCAACATTTAAGATTAAAAGGCCACAAGCGAAGGCTTATTTTGCAAAAGCCATATCGGACATGTATTCTGATCTTGCTATTCAGGAAAGATCTCTTCCTAGAAGTAGTTTGTGA___ATGCACAGAAGAAATTGTAATTGTACAAGAACCTAACCGTCACACATGTATGATCTGATTCATTTGTTAACTTAAGCATTAACTTGCATGGTTTCATACTAATTTCTTGCCCTTTTTGACTTTATGTTTGTATTATGTTGATTTCTTTAGCCCAAATGTAACTAAACTATTTGAGGAATCTTACCAAATCACCATGGACATGCTTCTAGTCATATAATGAGGGCTCCTTTCAATGAACTTTGATGCAAGGATAAAATATCCACCAAGGAAATTCATTAAATGTTTGTAAACTCTATGAATTATTGTCGGAGCCAAGCAATGATTCTATATACGCTAATTTAAGGAAATGATAAATCGAGTTTTGTATT

Fln Alignment:

sp_v1.1_unigene320___PRGEICVRGPIVFKGYFKDDVQTQEIF--DEEG--WLHTGDIGLWLPGGRLRIIDRKKNIFKLAQGEYIAPEKIENVYATCKFVGQCFIYGDSLNSSLXXXXXXEPDALKAWAMSEGIQVEDLSQLCSDPRAKEAVLADMDAVGREAQLRGFEFAKAVTLVAEPFSLENGLLTPTFKIKRPQAKAYFAKAISDMY
D5ACR7_____________PRGEIVIGGPHVTLGYFKNKEKTDESYKVDERGMRWFYTGDIGRFHPDGCLEIIDRKKDIVKLQHGEYVSLGKVEAALSVCHYLDNFMLHADPFHSYCVALVVPSRQALESWAQKEGIIYKDFSDLCQKSEAIKEVQSSILEVGKSAKLDKFEIPSKIKLLPDPWTPETGLVTAALKLKREVIKKTFAEELNKLY

SNPs (tot: 23)

Position	A %	C %	G %	T %	Probability
957	62		37		1.0
1297	76		23		1.0
1300	84		15		0.999986
1301	76	7		15	0.999983
1306			15	84	0.999986
1307			15	84	0.999986
1310		84	15		0.999986
1314	84			15	0.999986
1315		15	84		0.999986
1410	87		12		0.992554
1455	22		77		0.999999
1934	11			88	0.923564
1936	88		11		0.923564
1937	88		11		0.923564
1955	88			11	0.923564
2047			80	20	0.999996
2123		90		10	0.90298
2124	90		10		0.90298
2125			10	90	0.90298
2574	62		37		1.0
2651	22		77		0.999999
2741	87		12		0.992554
2830	50		50		1.0

SSRs (tot: 1)

Start position	End position	Sequence	Length
407	423	CAG CAG CAG CAG CAG CA	17

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Biología Molecular y Biotecnología de Plantas, Facultad de Ciencias y Plataforma Andaluza de Bioinformática, Universidad de Málaga, E-29071 Málaga, Spain